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21. 发明申请

US20080070285A1 Process for Producing Cmp-N-Acetylneuraminic Acid 失效
标题翻译：生产Cmp-N-乙酰神经氨酸的方法
公开(公告)号：US20080070285A1
公开(公告)日：2008-03-20
申请号：US10573385
申请日：2004-09-21
申请人： Tomoki Hamamoto , Kuniaki Nagaoka , Toshitada Noguchi
发明人： Tomoki Hamamoto , Kuniaki Nagaoka , Toshitada Noguchi
IPC分类号： C12P19/02
CPC分类号： C12P19/385 , C12P19/305
摘要： A method by which high-purity CMP-N-acetylneuraminic acid (HPLC purity, 95% or higher), which has been difficult to obtain with any technique other than chromatography, can be easily obtained in satisfactory yield by a simple operation without the need of chromatography. The process, which is for producing high-purity CMP-N-acetylneuraminic acid (CMP-NeuAc), is characterized by conducting a suitable combination of the following steps (1) to (4). Step 1: a step in which divalent cations are added to a solution containing CMP-NeuAc to thereby precipitate the phosphoric acid, pyrophosphoric acid, and nucleotide which coexist; Step 2: a step in which a phosphatase is added to a solution containing CMP-NeuAc to thereby convert the coexistent nucleotide into nucleoside; Step 3: a step in which an organic solvent is added to precipitate the CMP-NeuAc; and Step 4: a step in which the CMP-NeuAc precipitated is recovered.
摘要翻译：可以通过简单的操作容易地以令人满意的产率获得用色谱法以外的任何技术难以获得的高纯度CMP-N-乙酰神经氨酸（HPLC纯度，95％或更高）的方法，而不需要的色谱。用于生产高纯度CMP-N-乙酰神经氨酸（CMP-NeuAc）的方法的特征在于进行以下步骤（1）至（4）的适当组合。步骤1：将二价阳离子加入到含有CMP-NeuAc的溶液中以沉淀磷酸，焦磷酸和共存的核苷酸的步骤; 步骤2：将含有CMP-NeuAc的溶液中加入磷酸酶，从而将共存核苷酸转化为核苷的步骤; 步骤3：加入有机溶剂沉淀CMP-NeuAc的步骤; 步骤4：回收沉淀的CMP-NeuAc的步骤。

22. 发明授权

US07329522B2 Polyphosphate:AMP phosphotransferase 有权
标题翻译：多磷酸盐：AMP磷酸转移酶
公开(公告)号：US07329522B2
公开(公告)日：2008-02-12
申请号：US10514726
申请日：2003-05-28
申请人： Toshikazu Shiba , Toshitada Noguchi
发明人： Toshikazu Shiba , Toshitada Noguchi
IPC分类号： C12N9/12 , C12N1/20 , C12P19/30 , C12P21/06 , C07H21/04
CPC分类号： C12N9/1229 , C12N9/12 , C12P19/30 , C12P19/32 , C12P19/40
摘要： This invention relates to a novel polyphosphate: AMP phosphotransferase (PAP), a gene coding this PAP, and their use. The PAP has the following properties:(A) action: catalyzing of the following two reactions: NMP+PolyP(n)→NDP+PolyP(n-1) dNMP+POlyP(n)→dNDP+PolyP(n-1) (wherein NMP represents nucleoside monophosphate, NDP represents nucleoside diphosphate, dNMP represents deoxynucleoside monophosphate, dNDP represents deoxynucleoside diphosphate, n represents degree of polymerization of the polyphosphate which is an integer of up to 100); (B) substrate specificity: specific to AMP, GMP, IMP, dAMP, and dGMP, also acting with CMP, UMP, dCMP, and TMP;(C) molecular weight: about 55 to 56 Kd (kilodalton); and(D) specific activity: at least 70 units per 1 mg of enzyme protein.
摘要翻译：本发明涉及新型多磷酸盐：AMP磷酸转移酶（PAP），编码该PAP的基因及其用途。 PAP具有以下性质：（A）作用：催化以下两个反应：<？in-line-formula description =“In-line Formulas”end =“lead”？> NMP + PolyP（n） - > NDP + PolyP（n-1） <？in-line-formula description =“In-line Formulas”end =“tail”？> <？in-line-formula description =“In-Line Formulas”end =“lead”？> dNMP + POlyP（n） - > dNDP + PolyP（n-1）（其中NMP表示核苷单磷酸，NDP表示核苷二磷酸，dNMP表示脱氧核苷单磷酸，dNDP表示脱氧核苷二磷酸，n表示多磷酸酯的聚合度，整数高达100）; （B）底物特异性：特异于AMP，GMP，IMP，dAMP和dGMP，也与CMP，UMP，dCMP和TMP起作用; （C）分子量：约55至56Kd（千道尔顿）; 和（D）比活性：每1mg酶蛋白至少70个单位。

23. 发明授权

US08148112B2 Sugar chain containing 4-position halogenated galactose and application thereof 失效
标题翻译：含有4-位卤代半乳糖的糖链及其应用
公开(公告)号：US08148112B2
公开(公告)日：2012-04-03
申请号：US11815329
申请日：2006-02-14
申请人： Shin-Ichiro Nishimura , Noriko Nagahori , Tomoki Hamamoto , Kiyoshi Okuyama , Toshitada Noguchi
发明人： Shin-Ichiro Nishimura , Noriko Nagahori , Tomoki Hamamoto , Kiyoshi Okuyama , Toshitada Noguchi
IPC分类号： C12P19/50 , C12P19/52 , C12P19/26 , C07H15/00 , C07H5/04 , C07H5/06 , C08B37/00
CPC分类号： C12P19/32 , C07H15/08
摘要： The present invention is directed to, for example, an oligosaccharide having at an end thereof a 4-position halogenated galactose residue represented by formula (I): (wherein X represents a halogen atom, and R represents a monosaccharide, an oligosaccharide, or a carrier), a transferase inhibitor containing the oligosaccharide, and a method for inhibiting sugar chain elongation reaction in the presence of glycosyltransferase, the method including employing the inhibitor. The invention also provides a method for producing a 4-position halogenated galactose sugar nucleotide represented by formula (II): (wherein each of R1 to R3 represents a hydroxyl group, an acetyl group, a halogen atom, or a hydrogen atom; X represents a halogen atom; and M represents a hydrogen ion or a metal ion), wherein the method employs bacterium-derived galactokinase and bacterium-derived hexose-1-phosphate uridylyltransferase. The invention is also directed to a sugar chain containing 4-position halogenated galactose envisaged to be employed as drugs and other materials, and to applications of the compound.
摘要翻译：本发明涉及例如末端具有由式（I）表示的4位卤代半乳糖残基的寡糖：（其中X表示卤素原子，R表示单糖，寡糖或载体），含有寡糖的转移酶抑制剂，以及在糖基转移酶存在下抑制糖链延长反应的方法，该方法包括使用该抑制剂。本发明还提供了由式（II）表示的4-位卤代半乳糖糖核苷酸的制备方法：（式中，R 1〜R 3各自表示羟基，乙酰基，卤素原子或氢原子; X表示卤素原子; M表示氢离子或金属离子），其中该方法采用细菌来源的半乳糖激酶和细菌来源的己糖-1-磷酸尿苷酰转移酶。本发明还涉及设想用作药物和其它材料的4-位卤代半乳糖的糖链以及该化合物的应用。

24. 发明授权

US07901912B1 Method of producing uridine 5′-diphospho-N-acetylgalactosamine 有权
标题翻译：尿苷5'-二磷酸-N-乙酰半乳糖胺的制备方法
公开(公告)号：US07901912B1
公开(公告)日：2011-03-08
申请号：US11576837
申请日：2005-10-18
申请人： Kiyoshi Okuyama , Toshitada Noguchi
发明人： Kiyoshi Okuyama , Toshitada Noguchi
IPC分类号： C12P19/00 , C12P19/26 , C12P19/30 , C12P21/06 , C12N9/10 , C12N9/12 , C12N9/00 , C07H21/04 , C08B37/00
CPC分类号： C12P19/305 , C12N9/1241
摘要： The present invention provides a method for enzymatically producing uridine 5′-diphospho-N-acetylgalactosamine (UDP-GalNAc) (which is an important substrate for oligosaccharide synthesis) from uridine 5′-triphosphate (UTP) and N-acetylgalactosamine 1-phosphate (GalNAc 1-P), the method including using, as an enzyme, uridine 5′-diphospho-N-acetylglucosamine pyrophosphorylase (UDP-GlcNAc pyrophosphorylase) derived from a microorganism (exclusive of a pathogenic microorganism). The GalNAc 1-P employed can be prepared from N-acetylgalactosamine and a phosphate donor in a reaction system by use of N-acetylgalactosamine kinase. According to the present invention, uridine 5′-diphospho-N-acetylgalactosamine can be efficiently produced by use of a relatively inexpensive substrate.
摘要翻译：本发明提供了一种从尿苷5'-三磷酸（UTP）和N-乙酰半乳糖胺-1-磷酸酯（UTP）酶促产生尿苷5'-二磷酸-N-乙酰半乳糖胺（UDP-GalNAc）（其是寡糖合成的重要底物） GalNAc 1-P），该方法包括使用来自微生物（不包括病原微生物）的尿苷5'-二磷酸-N-乙酰葡糖胺焦磷酸化酶（UDP-GlcNAc焦磷酸化酶）作为酶。使用的GalNAc 1-P可以通过使用N-乙酰半乳糖胺激酶在反应体系中由N-乙酰半乳糖胺和磷酸供体制备。根据本发明，可以通过使用相对便宜的底物有效地制备尿苷5'-二磷酸-N-乙酰半乳糖胺。

25. 发明授权

US6022713A Process for producing nucleoside 5'-triphosphates and application of the same 失效
标题翻译：制备核苷5'-三磷酸的方法及其应用
公开(公告)号：US6022713A
公开(公告)日：2000-02-08
申请号：US101683
申请日：1998-07-15
申请人： Toshitada Noguchi , Toshikazu Shiba
发明人： Toshitada Noguchi , Toshikazu Shiba
IPC分类号： C12N9/12 , C12N9/90 , C12P19/30 , C12P19/00 , C12P19/18 , C12P19/26
CPC分类号： C12N9/1229 , C12N9/1241 , C12N9/90 , C12P19/30 , C12Y207/04001 , C12Y207/07009 , C12Y501/03002 , Y02P20/582
摘要： The present invention relates to a process for producing nucleoside 5'-triphosphates (NTP) from nucleoside 5'-diphosphates (NDP) other than adenosine 5'-diphosphate (ADP), characterized by using a polyphosphate kinase as an enzyme and polyphosphate as the phosphate donor; and application of this process to various glycosylation reactions.This process makes it possible to conveniently and economically synthesize NTP from NDP enzymatically. Also, it becomes possible thereby to economically recycle and synthesize sugar nucleotides and synthesize, for example, oligosaccharides associating therewith without resort to expensive phosphoenol pyruvate, ATP, etc. in the reactions for reproducing or converting NDP into NTP in the systems for enzymatically synthesizing oligosaccharides by combining, for example, with the synthesis of sugar nucleotides.
摘要翻译： PCT No.PCT / JP97 / 04159 Sec。 371日期：1998年7月15日 102（e）日期1998年7月15日PCT 1997年11月14日PCT PCT。第WO98 / 22614号公报日期1998年5月28日本发明涉及一种由5'-二磷酸腺苷（ADP）以外的核苷5'-二磷酸（NDP）生产核苷5'-三磷酸（NTP）的方法，其特征在于使用多磷酸激酶作为酶和多磷酸盐作为磷酸盐供体; 并将该方法应用于各种糖基化反应。该方法使得可以方便和经济地从NDP酶合成NTP。另外，可以经济地循环和合成糖核苷酸，并合成例如在与低聚糖合成的系统中用于再生或将NDP再生或转化成NTP的反应中，与昂贵的磷酸烯醇丙酮酸，ATP等缔合而成的寡糖通过例如与糖核苷酸的合成组合。

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