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21. 发明授权

US07915230B2 Reagents for transfection of eukaryotic cells 有权
标题翻译：用于转染真核细胞的试剂
公开(公告)号：US07915230B2
公开(公告)日：2011-03-29
申请号：US11434765
申请日：2006-05-17
申请人： Joel Jessee , Gulilat Gebeyehu
发明人： Joel Jessee , Gulilat Gebeyehu
IPC分类号： A61K48/00 , A61K38/16 , C12N15/00
CPC分类号： C07K14/005 , A61K47/6455 , C12N15/87 , C12N2720/12022
摘要： Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.
摘要翻译：提供了将大分子转运到真核细胞中的组合物和方法。来自非包膜病毒融合蛋白的融合肽提高了转染试剂如阳离子脂质，聚阳离子聚合物如PEI和树枝状大分子介导的真核细胞的转染效率。这些融合肽用作转染复合物的一部分，其将大分子例如核酸有效地递送到真核细胞中。还提供了新的阳离子脂质和阳离子脂质的组合物，其可用于将大分子如核酸，蛋白质和肽引入多种细胞和组织中。脂质可以单独使用，与其他脂质组合使用和/或与融合肽组合使用以制备转染复合物。

22. 发明授权

US12043639B2 Polycationic methyl phospholipids for improved delivery of nucleic acids to eukaryotic cells 有权
公开(公告)号：US12043639B2
公开(公告)日：2024-07-23
申请号：US17222442
申请日：2021-04-05
申请人： MOLECULAR TRANSFER, INC.
发明人： Gulilat Gebeyehu , Joel Jessee
IPC分类号： C07F9/10 , C07F9/6506 , C12N15/113 , C12N15/88 , C12P21/00
CPC分类号： C07F9/10 , C07F9/106 , C07F9/6506 , C12N15/113 , C12N15/88 , C12P21/00 , C12N2310/14
摘要： New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.

23. 发明授权

US11085056B2 Transfection complexes and methods of using the same 有权
公开(公告)号：US11085056B2
公开(公告)日：2021-08-10
申请号：US15756006
申请日：2016-08-26
申请人： MOLECULAR TRANSFER, INC.
发明人： Gulilat Gebeyehu , Joel Jessee
IPC分类号： A61K38/00 , C12N15/87 , C07K7/06 , C07K7/08 , C07K14/00 , C07K5/097 , C07K5/00 , C07K5/072 , C07K5/09 , C07K7/04 , C07K5/083 , C07K5/068 , C07K5/087 , C07K5/11
摘要： Disclosed herein are transfection complexes comprising at least one cell surface ligand; at least one helper lipid component; and a transfection enhancer. Also disclosed are pharmaceutical compositions comprising the disclosed transfection complexes, and a pharmaceutically acceptable carrier. Further, disclosed are methods of transfecting a cell, the method comprising the steps of: obtaining a transfection complex as disclosed; and contacting a cell with the transfection complex.

24. 发明授权

US10883118B2 Agents for improved delivery of nucleic acids to eukaryotic cells 有权
公开(公告)号：US10883118B2
公开(公告)日：2021-01-05
申请号：US16197989
申请日：2018-11-21
申请人： Molecular Transfer Inc.
发明人： Joel Jessee , Gulilat Gebeyehu
IPC分类号： C12N15/88 , A61K31/195 , C07C217/08 , A61K9/127 , A61K31/713 , C12N15/113 , C07C237/10 , C07D233/64 , C07C237/12
摘要： New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.

25. 发明授权

US10155780B2 Polycationic methyl phospholipids for improved delivery of nucleic acids to eukaryotic cells 有权
公开(公告)号：US10155780B2
公开(公告)日：2018-12-18
申请号：US15107889
申请日：2013-10-28
申请人： MOLECULAR TRANSFER, INC.
发明人： Gulilat Gebeyehu , Joel Jessee
IPC分类号： C07F9/10 , C12N15/113 , C12N15/88 , C12P21/00 , C07F9/6506
摘要： New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes. The present invention is in the field of molecular biology, and more particularly in the field of transfection, which utilizes new compounds and methods for the introduction of nucleic acids into eukaryotic cells.

26. 发明申请

US20180340188A1 TRANSFECTION COMPLEXES AND METHODS OF USING THE SAME 审中-公开
公开(公告)号：US20180340188A1
公开(公告)日：2018-11-29
申请号：US15756006
申请日：2016-08-26
申请人： MOLECULAR TRANSFER, INC.
发明人： Gulilat GEBEYEHU , Joel JESSE
IPC分类号： C12N15/87 , C07K7/08 , C07K7/06 , C07K14/00
摘要： Disclosed herein are transfection complexes comprising at least one cell surface ligand; at least one helper lipid component; and a transfection enhancer. Also disclosed are pharmaceutical compositions comprising the disclosed transfection complexes, and a pharmaceutically acceptable carrier. Further, disclosed are methods of transfecting a cell, the method comprising the steps of: obtaining a transfection complex as disclosed; and contacting a cell with the transfection complex.

27. 发明授权

US09259475B2 Agents for improved delivery of nucleic acids to eukaryotic cells 有权
标题翻译：用于将核酸转运至真核细胞的药剂
公开(公告)号：US09259475B2
公开(公告)日：2016-02-16
申请号：US14054825
申请日：2013-10-16
申请人： Molecular Transfer Inc.
发明人： Joel Jessee , Gulilat Gebeyehu
IPC分类号： A61K9/127 , C12N15/00 , C07H21/04 , C07H21/02 , A61K47/48 , A61K31/195 , C07C237/10 , C07D233/64 , C07C217/08 , C12N15/88
CPC分类号： C12N15/88 , A61K9/1272 , A61K31/195 , A61K31/713 , C07C217/08 , C07C237/10 , C07C237/12 , C07D233/64 , C12N15/1137 , C12N2310/14 , C12N2310/3515 , C12N2320/32
摘要： New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
摘要翻译：提供了新的阳离子脂质，其可用于将大分子如核酸递送到真核细胞中。脂质可以单独使用，与其他脂质组合使用，和/或与其他转染增强试剂组合使用以制备转染复合物。

28. 发明申请

US20120156786A1 READY-TO-USE ELECTROPORATION CUVETTE INCLUDING FROZEN ELECTROCOMPETENT CELLS 审中-公开
标题翻译：准备使用电冰箱，包括冷冻电解质电池
公开(公告)号：US20120156786A1
公开(公告)日：2012-06-21
申请号：US13353052
申请日：2012-01-18
申请人： Robert L. Bebee
发明人： Robert L. Bebee
IPC分类号： C12N15/63 , C12M1/42
CPC分类号： C12M35/02
摘要： A ready-to-use electroporation cuvette is provided that includes a cuvette, first and second electrodes positioned within the cuvette and electroporation competent cells frozen in a suspension solution within the cuvette, wherein the electroporation cuvette is configured to permit electroporation of the cells when the cells are thawed. The electroporation cuvette may be sealed with a cap that may be color coded to aid the user.
摘要翻译：提供了一种现成的电穿孔试管，其包括比色皿，位于比色皿内的第一和第二电极以及冷藏在比色杯内的悬浮溶液中的电穿孔感受态细胞，其中电穿孔比色皿构造成允许电穿孔细胞解冻。电穿孔试管可以用可以用颜色编码的帽来密封以帮助使用者。

29. 发明申请

US20120052574A1 NOVEL REAGENTS FOR TRANSFECTION OF EUKARYOTIC CELLS 有权
标题翻译：用于转染真核细胞的新型试剂
公开(公告)号：US20120052574A1
公开(公告)日：2012-03-01
申请号：US13073972
申请日：2011-03-28
申请人： Joel Jessee , Gulilat Gebeyehu
发明人： Joel Jessee , Gulilat Gebeyehu
IPC分类号： C12N5/02 , C07C229/04 , C07K14/14 , C07D241/04 , C07K7/06 , C07K7/08
CPC分类号： C07K14/005 , A61K47/6455 , C12N15/87 , C12N2720/12022
摘要： Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.
摘要翻译：提供了将大分子转运到真核细胞中的组合物和方法。来自非包膜病毒融合蛋白的融合肽提高了转染试剂如阳离子脂质，聚阳离子聚合物如PEI和树枝状大分子介导的真核细胞的转染效率。这些融合肽用作转染复合物的一部分，其将大分子例如核酸有效地递送到真核细胞中。还提供了新的阳离子脂质和阳离子脂质的组合物，其可用于将大分子如核酸，蛋白质和肽引入多种细胞和组织中。脂质可以单独使用，与其他脂质组合使用和/或与融合肽组合使用以制备转染复合物。

30. 发明授权

US08034977B2 Lipids for transfection of nucleic acids 有权
标题翻译：用于转染核酸的脂质
公开(公告)号：US08034977B2
公开(公告)日：2011-10-11
申请号：US12430747
申请日：2009-04-27
申请人： Gulilat Gebeyehu , Taysir M. Jaouni , Joel Jessee
发明人： Gulilat Gebeyehu , Taysir M. Jaouni , Joel Jessee
IPC分类号： C07C211/63 , A61K31/14 , C12N15/88
CPC分类号： C07C217/28 , C07C215/14
摘要： Cationic lipid compositions are provided that are useful for efficient delivery of macromolecules, such as nucleic acids, into a wide variety of eukaryotic cell types. Methods for using the compositions also are provided.
摘要翻译：提供了阳离子脂质组合物，其可用于将大分子如核酸有效递送到各种真核细胞类型中。还提供了使用组合物的方法。

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