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21. 发明申请

US20060019313A1 Method for detecting molecular surface interactions 审中-公开
标题翻译：检测分子表面相互作用的方法
公开(公告)号：US20060019313A1
公开(公告)日：2006-01-26
申请号：US11158898
申请日：2005-06-22
申请人： Karl Andersson , Anna Moberg
发明人： Karl Andersson , Anna Moberg
IPC分类号： G01N33/53
CPC分类号： G01N33/54393 , G01N33/54373
摘要： A method for determining interaction of an analyte with a binding agent immobilized to a solid support surface by contacting the surface with a fluid sample containing the analyte, and detecting binding events at the solid support surface, wherein the sample is based on a complex medium containing at least one species other than analyte that may interact with the solid support surface, is disclosed. The method comprises contacting the solid support surface with sample medium free from analyte prior to contacting the surface with the analyte-containing sample.
摘要翻译：一种用于通过使表面与含有分析物的流体样品接触并检测固体支持物表面的结合事件来确定分析物与固定在固体支持物表面上的结合剂的相互作用的方法，其中所述样品基于含有公开了至少一种可与固体支持物表面相互作用的分析物以外的物质。该方法包括在将表面与含分析物的样品接触之前使固体支持体表面与不含分析物的样品介质接触。

22. 发明申请

US20060014232A1 Immobilization method 审中-公开
标题翻译：固定方法
公开(公告)号：US20060014232A1
公开(公告)日：2006-01-19
申请号：US10535736
申请日：2003-09-22
申请人： Junichi Inagawa , Noriyuki Inomata
发明人： Junichi Inagawa , Noriyuki Inomata
IPC分类号： C12Q1/37 , C12N11/04
CPC分类号： G01N33/54353 , C12Q1/6825 , C12Q1/6834 , C12Q2565/628
摘要： Various biomolecules can be firmly immobilized to a substrate by a method which comprises contacting a solution containing biomolecules provided with at least one tag with an immobilization substrate which has (i) binding sites for the biomolecule tag or tags, and (ii) activated reactive groups which are capable of forming covalent bonds with the biomolecules, to covalently bind the biomolecules interacting with the tag binding sites to the immobilization substrate.
摘要翻译：通过包括将含有至少一个标签的生物分子的溶液与具有（i）生物分子标签或标签的结合位点的固定化底物和（ii）活化的反应性基团的固定化底物的方法将各种生物分子牢固地固定在底物上其能够与生物分子形成共价键，以将与标签结合位点相互作用的生物分子共价结合至固定化底物。

23. 发明申请

US20050062974A1 Apparatus and method for total internal reflection spectroscopy 失效
标题翻译：全内反射光谱仪的装置和方法
公开(公告)号：US20050062974A1
公开(公告)日：2005-03-24
申请号：US10869824
申请日：2004-06-16
申请人： Bengt Ivarsson
发明人： Bengt Ivarsson
IPC分类号： G01J20060101 , G01N21/55
CPC分类号： G01N21/552 , G01N21/553 , G01N21/648
摘要： An optical apparatus for total internal reflection spectroscopy comprises: a transparent body having an internally reflective surface; at least one source of electromagnetic radiation for providing at least one beam of collimated electromagnetic radiation; optical scanning means for directing the beam or beams to the transparent body so that the radiation is internally reflected at the reflective surface, and sequentially or continuously scanning the incident angle of the radiation over an angular range; at least one detector for detecting electromagnetic radiation exiting the transparent body, and means for counteracting variation of the irradiance in the illuminated area of the surface during the angular scan, or the effect of such variation on the reflected beam or beams. An optical apparatus for examining thin layer structures on a surface for differences in respect of optical thickness and/or refractive index, and a method for total internal reflection spectroscopy are also disclosed.
摘要翻译：用于全内反射光谱的光学装置包括：具有内部反射表面的透明体; 用于提供至少一个准直电磁辐射束的至少一个电磁辐射源; 用于将光束或光束引导到透明体的光学扫描装置，使得辐射在反射表面处被内部反射，并且在角度范围内依次或连续地扫描辐射的入射角; 用于检测离开透明体的电磁辐射的至少一个检测器，以及用于在角扫描期间抵消表面的照射区域中的辐照度变化的装置，或这种变化对反射光束或束的影响的装置。还公开了一种用于检查表面上关于光学厚度和/或折射率的差异的薄层结构的光学装置，以及全内反射光谱法的方法。

24. 发明授权

US06844201B2 Method for capturing analytes eluted from surface-bound ligands 有权
标题翻译：用于捕获从表面结合配体洗脱的分析物的方法
公开(公告)号：US06844201B2
公开(公告)日：2005-01-18
申请号：US10295709
申请日：2002-11-15
申请人： Magnus Malmqvist , Östen Jansson
发明人： Magnus Malmqvist , Östen Jansson
IPC分类号： G01N33/531 , B01J20/281 , B01J20/283 , B01J20/291 , G01N21/55 , G01N30/26 , G01N30/88 , G01N33/537 , G01N33/543 , G01N33/548 , G01N33/552 , G01N33/553 , G01N33/566
CPC分类号： G01N33/537 , G01N21/553 , G01N21/648 , G01N33/543 , G01N33/54313
摘要： Methods for capturing analytes associated with surface-bound ligands are disclosed. The methods involve eluting analytes from surface-bound ligands with a first liquid to generate free analytes, and capturing the free analytes with a solid capturing material within the first liquid to generate a first liquid containing captured analytes. The first liquid may be a flowing liquid or a non-flowing liquid, and the surface to which the surface-bound ligand is attached may be a sensing surface, such as a biosensor, or a non-sensing surface. The captured analytes may be further consolidated at a location removed from the surface-bound ligand, eluted from the solid capturing material with a second liquid, and used for subsequent analysis or procedures.
摘要翻译：公开了捕获与表面结合配体相关的分析物的方法。该方法包括用第一液体洗脱来自表面结合的配体的分析物以产生游离的分析物，以及用第一液体内的固体捕获材料捕获游离的分析物，以产生含有捕获的分析物的第一液体。第一液体可以是流动的液体或不流动的液体，并且表面结合的配体附着的表面可以是感测表面，例如生物传感器或非感测表面。捕获的分析物可以在从表面结合的配体去除的位置进一步固结，用固体捕获材料用第二液体洗脱，并用于随后的分析或程序。

25. 发明申请

US20050007605A1 Analytical method and apparatus 失效
标题翻译：分析方法和装置
公开(公告)号：US20050007605A1
公开(公告)日：2005-01-13
申请号：US10766696
申请日：2004-01-27
申请人： Bengt Ivarsson
发明人： Bengt Ivarsson
IPC分类号： G01N33/543 , G01B11/06 , G01N21/27 , G01N21/55 , G01B11/28
CPC分类号： G01B11/0625 , G01N21/552 , G01N21/553 , G01N2021/212 , G01N2021/215
摘要： A method of examining thin layer structures on a surface for differences in respect of optical thickness, which method comprises the steps of: irradiating the surface with light so that the light is internally or externally reflected at the surface; imaging the reflected light on a first two-dimensional detector; sequentially or continuously scanning the incident angle and/or wavelength of the light over an angular and/or wavelength range; measuring the intensities of light reflected from different parts of the surface and impinging on different parts of the detector, at at least a number of incident angles and/or wavelengths, the intensity of light reflected from each part of the surface for each angle and/or wavelength depending on the optical thickness of the thin layer structure thereon; and determining from the detected light intensities at the different light incident angles and/or wavelengths an optical thickness image of the thin layer structures on the surface. According to the invention, part of the light reflected at said surface is detected on a second detector to determine the incident angle or wavelength of the polarized light irradiating the surface. An apparatus for carrying out the method is also disclosed.
摘要翻译：一种检查表面上薄层结构相对于光学厚度差异的方法，该方法包括以下步骤：用光照射表面，使得光在表面内部或外部反射; 对第一二维检测器上的反射光进行成像; 在角度和/或波长范围内顺序地或连续地扫描光的入射角和/或波长; 测量从表面的不同部分反射的光的强度，并且以至少多个入射角和/或波长在入射角和/或波长的至少多个入射角度和/或波长处撞击从表面的每个部分反射的光的强度和/ 或波长取决于其上的薄层结构的光学厚度; 并且根据检测到的不同光入射角的光强度和/或波长确定表面上的薄层结构的光学厚度图像。根据本发明，在第二检测器上检测在所述表面反射的光的一部分，以确定照射表面的偏振光的入射角或波长。还公开了一种用于执行该方法的装置。

26. 发明申请

US20040002167A1 Method and system for curve quality control 有权
标题翻译：曲线质量控制方法和系统
公开(公告)号：US20040002167A1
公开(公告)日：2004-01-01
申请号：US10400158
申请日：2003-03-26
申请人： Biacore AB
发明人： Karl Andersson , Peter Borg
IPC分类号： G01N033/543
CPC分类号： G01N33/54373 , G06F19/18 , G06F19/24
摘要： A method of analysis wherein molecular interactions at one or more sensing surface areas are detected and respective response curves representing the progress of each interaction with time are produced, and wherein a resulting set of response curves is subjected to a quality assessment procedure which comprises representing the response curves with one or more quality descriptors, applying a quality classification method to the descriptors to find outliers, and removing the outliers. The invention also relates to an analytical system including means for classifying the response curves with regard to quality, a computer program for performing the classification, and a computer program product containing the program.
摘要翻译：一种分析方法，其中检测一个或多个感测表面区域处的分子相互作用，并且产生表示每次与时间相互作用的进展的各自的响应曲线，并且其中对所得到的一组响应曲线进行质量评估程序，其包括表示响应曲线与一个或多个质量描述符，向描述符应用质量分类方法以找出异常值，并移除异常值。本发明还涉及一种分析系统，包括用于对质量的响应曲线进行分类的装置，用于执行分类的计算机程序以及包含该程序的计算机程序产品。

27. 发明授权

US5955729A Surface plasmon resonance-mass spectrometry 失效
标题翻译：表面等离子体共振质谱
公开(公告)号：US5955729A
公开(公告)日：1999-09-21
申请号：US708341
申请日：1996-09-06
申请人： Randall W. Nelson , Jennifer R. Krone , Russell Granzow , Osten Jansson , Stefan Sjolander
发明人： Randall W. Nelson , Jennifer R. Krone , Russell Granzow , Osten Jansson , Stefan Sjolander
IPC分类号： G01N27/62 , G01N21/27 , G01N21/55 , G01N33/543 , B01D59/44 , H01J49/00
CPC分类号： H01J49/0418 , G01N21/553 , H01J49/164
摘要： The invention provides surface plasmon resonance-mass spectroscopy for the rapid, sensitive and accurate investigation of molecular interactions coupled with the identification and quantification of the same. Methods of the invention include capturing an analyte present within a sample by an interactive surface layer on a real-time interaction analysis sensor, analyzing the analyte by surface plasmon resonance while the analyte is captured by the interactive surface layer, and identifying the captured analyte by desorbing/ionizing the analyte from the interactive surface layer while under vacuum within a mass spectrometer. Devices of the invention include a transparent material, a conductive material capable of supporting surface plasmon resonance affixed to the transparent material, an interactive surface affixed to the conductive material, and a means for exposing the interactive surface to the interior of a mass spectrometer without breaking the vacuum therein.
摘要翻译：本发明提供表面等离子体共振 - 质谱法，用于快速，灵敏和准确地研究分子相互作用以及其鉴定和定量。本发明的方法包括通过实时相互作用分析传感器上的交互表面层捕获样品中存在的分析物，通过表面等离子体共振分析分析物，同时通过交互表面层捕获分析物，并通过在质谱仪内真空下从交互表面层解离/电离分析物。本发明的装置包括透明材料，能够支撑固定在透明材料上的表面等离子体共振的导电材料，固定在导电材料上的相互作用表面，以及用于将相互作用表面暴露于质谱仪内部而不断裂的装置其中的真空。

28. 发明授权

US07373255B2 Method and system for determination of molecular interaction parameters 有权
标题翻译：测定分子相互作用参数的方法和系统
公开(公告)号：US07373255B2
公开(公告)日：2008-05-13
申请号：US10861098
申请日：2004-06-04
申请人： Robert Karlsson , Karl Andersson , Christina Wass
发明人： Robert Karlsson , Karl Andersson , Christina Wass
IPC分类号： G01N7/00
CPC分类号： G01N33/6854 , B01L3/5027 , G01N33/53 , G01N33/543 , G01N35/08 , G01N2333/912 , G01N2333/936 , G01N2333/974 , G01N2333/988 , G01N2500/04 , G01N2500/20 , Y10S707/99943
摘要： A method of determining kinetic parameters for a reversible molecular interaction between a ligand immobilized to a solid support surface and a binding partner to the ligand in solution, comprises sequentially, without intermediate regeneration or renewal of the immobilized ligand, flowing a plurality of fluid volumes containing different known concentrations of the binding partner over the solid support surface, monitoring the momentary amount of binding partner bound to the solid support surface related to time and solution concentration of binding partner and collecting the binding data, and determining the kinetic parameters by globally fitting a predetermined kinetic model for the interaction between the binding partner and the immobilized ligand to the collected binding data, which model allows for mass transport limitation at the solid support surface. An analytical system for carrying out the method, a computer program, a computer program product and a computer system for performing the method are also disclosed.
摘要翻译：确定固定在固体支持物表面的配体与溶液中配体的结合配偶体之间的可逆分子相互作用的动力学参数的方法包括顺序地，不经过固定配体的中间再生或更新，流过多个含有在固体支持物表面上结合配偶体的不同已知浓度，监测与固体支持物表面结合的结合配偶体的瞬时量与结合配偶体的时间和溶液浓度相关并收集结合数据，并通过全局拟合来确定动力学参数用于结合配偶体和固定的配体与收集的结合数据之间相互作用的预定动力学模型，该模型允许在固体支持物表面的质量传递限制。还公开了用于执行该方法的分析系统，计算机程序，计算机程序产品和用于执行该方法的计算机系统。

29. 发明授权

US07219528B2 Sample flow positioning method and analytical system using the method 有权
标题翻译：采样流定位方法及分析系统采用该方法
公开(公告)号：US07219528B2
公开(公告)日：2007-05-22
申请号：US11005492
申请日：2004-12-06
申请人： Mattias Tidare , Roos Håkan
发明人： Mattias Tidare , Roos Håkan
IPC分类号： G01N21/00
CPC分类号： G05D7/0682 , G01N21/553 , Y10T436/25
摘要： A method of positioning a laminar flow of a sample fluid on a surface within a flow cell is disclosed. The method comprises providing a laminar flow of a first guiding fluid adjacent to a laminar flow of a second guiding fluid different from the first guiding fluid such that the two fluids flow together over the surface with a detectable interface to each other; detecting the interface between the two different guiding fluids; optionally adjusting the interface laterally to a desired position by adjusting the relative flow rates of the two guiding fluids; and introducing a laminar flow of the sample fluid between the laminar flows of the first and second guiding fluids such that the flow of the sample fluid is sandwiched between the guiding fluids. An analytical system for carrying out the method, a computer program, a computer program product and a computer system for performing the method are also disclosed.
摘要翻译：公开了一种将样品流体的层流定位在流动池内的表面上的方法。该方法包括提供与第一引导流体不同的第二引导流体的层流相邻的第一引导流体的层流，使得两个流体在表面上一起流动，彼此具有可检测的界面; 检测两种不同导向流体之间的界面; 可选地通过调节两个引导流体的相对流速来将界面侧向调节到所需位置; 以及在第一和第二引导流体的层流之间引入样品流体的层流，使得样品流体的流动被夹在引导流体之间。还公开了用于执行该方法的分析系统，计算机程序，计算机程序产品和用于执行该方法的计算机系统。

30. 发明授权

US07081958B2 Analytical method and apparatus 失效
公开(公告)号：US07081958B2
公开(公告)日：2006-07-25
申请号：US11234829
申请日：2005-09-23
申请人： Bengt Ivarsson
发明人： Bengt Ivarsson
IPC分类号： G01N21/55 , G01B11/28
CPC分类号： G01B11/0625 , G01N21/552 , G01N21/553 , G01N2021/212 , G01N2021/215
摘要： A method of examining thin layer structures on a surface for differences in respect of optical thickness, which method comprises the steps of: irradiating the surface with light so that the light is internally or externally reflected at the surface; imaging the reflected light on a first two-dimensional detector; sequentially or continuously scanning the incident angle and/or wavelength of the light over an angular and/or wavelength range; measuring the intensities of light reflected from different parts of the surface and impinging on different parts of the detector, at at least a number of incident angles and/or wavelengths, the intensity of light reflected from each part of the surface for each angle and/or wavelength depending on the optical thickness of the thin layer structure thereon; and determining from the detected light intensities at the different light incident angles and/or wavelengths an optical thickness image of the thin layer structures on the surface. According to the invention, part of the light reflected at said surface is detected on a second detector to determine the incident angle or wavelength of the polarized light irradiating the surface. An apparatus for carrying out the method is also disclosed.

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