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11. 发明申请

US20120084884A1 STABLY TRANSFORMED FERNS AND RELATED METHODS 审中-公开
标题翻译： STABLY变压器及相关方法
公开(公告)号：US20120084884A1
公开(公告)日：2012-04-05
申请号：US13253659
申请日：2011-10-05
申请人： Neal Stewart , Muthukumar Balasubramaniam , Blake Joyce
发明人： Neal Stewart , Muthukumar Balasubramaniam , Blake Joyce
IPC分类号： A01H9/00 , C12N15/82
CPC分类号： C12N15/8205 , C12N15/8207 , C12N15/8216
摘要： Methods that provide for stable transformation of fern spores and/or protonemata by Agrobacterium- and/or by particle-mediated transformation are disclosed. Also provided are stably transformed, non-chimeric ferns.
摘要翻译：公开了通过农杆菌和/或通过颗粒介导的转化提供蕨类孢子和/或质子稳定转化的方法。还提供了稳定转化的非嵌合蕨类植物。

12. 发明授权

US08008061B2 Trophic conversion of obligate phototrophic algae through metabolic engineering 失效
标题翻译：通过代谢工程营养转化专性光营养藻类
公开(公告)号：US08008061B2
公开(公告)日：2011-08-30
申请号：US11842898
申请日：2007-08-21
申请人： Kirk Emil Apt , F. C. Thomas Allnutt , David J. Kyle , James Casy Lippmeier
发明人： Kirk Emil Apt , F. C. Thomas Allnutt , David J. Kyle , James Casy Lippmeier
IPC分类号： C12N1/12
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
摘要翻译：大多数微藻是专性光自养体，其生长严格依赖于光合作用衍生能量的产生。在这项研究中，显示微藻Phaeodaclylurn三角褐豆可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。人和小球藻凯斯勒葡萄糖转运蛋白都促进了三角褐指藻的葡萄糖摄取，使得细胞代谢外源有机碳，并且独立于光。这是通过代谢工程首次成功地营养专一性自养型营养转化，并且表明通过引入单一基因可以从根本上改变细胞营养的方法。由于用葡萄糖转运基因转化的菌株能够非光合作用生长，因此可以通过突变体的产生和表征来开发光合作用的分析。最后，这项工作也是通过使用微生物发酵技术大规模商业开发专利性光营养藻类的重要进展，消除了光依赖性生长造成的重大限制。

13. 发明申请

US20100285591A1 CYCLIN D POLYNUCLEOTIDES, POLYPEPTIDES AND USES THEREOF 审中-公开
标题翻译： CYCLIN D多核苷酸，多肽及其用途
公开(公告)号：US20100285591A1
公开(公告)日：2010-11-11
申请号：US12844220
申请日：2010-07-27
申请人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , John A. McElver , George J. Hoerster , Sheila E. Maddock
发明人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , John A. McElver , George J. Hoerster , Sheila E. Maddock
IPC分类号： C12N15/82 , C07H21/04 , C12N15/63 , C12N5/10
CPC分类号： C12N15/8209 , C07K14/415 , C12N15/8201 , C12N15/8206 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated polynucleotides, specifically Cyclin D polynucleotides, and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants for the purpose of increasing transformation efficiency.
摘要翻译：本发明提供分离的多核苷酸，特别是细胞周期蛋白D多核苷酸及其编码的参与细胞周期调节的蛋白质。本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。本发明提供了改变植物的细胞周期蛋白质含量和/或组成以提高转化效率的方法和组合物。

14. 发明申请

US20100235942A1 METHODS FOR CORN TRANSFORMATION 有权
标题翻译： CORN转换方法
公开(公告)号：US20100235942A1
公开(公告)日：2010-09-16
申请号：US12729072
申请日：2010-03-22
申请人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
发明人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
IPC分类号： A01H5/00 , A01H4/00 , C12N15/82
CPC分类号： C12N15/8207 , C12N15/8205 , C12N15/8206 , C12N15/8209
摘要： The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.
摘要翻译：本发明涉及一种用于产生转化玉米植物的新型转化系统。特别地，本发明涉及一种在高温下的快速选择系统，其允许更快和更有效的转化。

15. 发明授权

US07348468B1 Wuschel (wus) gene homologs 有权
标题翻译： Wuschel（wus）基因同源物
公开(公告)号：US07348468B1
公开(公告)日：2008-03-25
申请号：US09807946
申请日：2000-09-28
申请人： Rebecca E. Cahoon , Keith Lowe , William James Gordon-Kamm , Yumin Tao , Christopher Jay Scelonge
发明人： Rebecca E. Cahoon , Keith Lowe , William James Gordon-Kamm , Yumin Tao , Christopher Jay Scelonge
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding a WUS protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the WUS protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the WUS protein in a transformed host cell.
摘要翻译：本发明涉及编码WUS蛋白的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码WUS蛋白的全部或一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中WUS蛋白水平的改变。

16. 发明申请

US20070300315A1 Fertile transgenic corn plants 审中-公开
标题翻译：可育转基因玉米植物
公开(公告)号：US20070300315A1
公开(公告)日：2007-12-27
申请号：US11555860
申请日：2006-11-02
申请人： Ronald Lundquist , David Walters
发明人： Ronald Lundquist , David Walters
IPC分类号： C12N15/82
CPC分类号： C07K14/325 , C07K14/425 , C12N15/8207 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8274 , C12N15/8286 , Y02A40/162
摘要： Fertile transgenic Zea mays (corn) plants which stably express heterologous DNA which is heritable are disclosed along with a process for producing said plants. The process comprises the microjectile bombardment of friable embryogenic callus from the plant to be transformed. The process may be applicable to other graminaceous cereal plants which have not proven stably transformable by other techniques.
摘要翻译：与生产所述植物的方法一起公开了稳定表达可遗传的异源DNA的可育转基因玉米（玉米）植物。该过程包括从待转化的植物中的脆性胚发生愈伤组织的微孔轰击。该方法可适用于尚未被其他技术稳定转化的其他禾本科谷物植物。

17. 发明申请

US20070276640A1 METHODS FOR CLASSIFICATION OF SOMATIC EMBRYOS 审中-公开
标题翻译： SOMATIC EMBRYOS分类方法
公开(公告)号：US20070276640A1
公开(公告)日：2007-11-29
申请号：US11836095
申请日：2007-08-08
申请人： Roger Timmis , Mitchell Toland , Timnit Ghermay , William Carlson , James Grob
发明人： Roger Timmis , Mitchell Toland , Timnit Ghermay , William Carlson , James Grob
IPC分类号： A01H7/00 , G06G7/58
CPC分类号： G06K9/00147 , A01C1/00 , A01H1/04 , A01H4/00 , B07C2501/009 , C12N15/8207
摘要： The present invention is directed towards methods for the classification of plant embryos by the application of one or more classification algorithms to analyze digitized images and absorption, transmittance, or reflectance spectra. The methods are generally applicable and emphasize the importance of acquiring and using as much image and absorption, transmittance, or reflectance spectral information as possible, based on objective criteria. The present invention allows automated selection of embryos most suitable for further culture and rejection of those seen as less suitable.
摘要翻译：本发明涉及通过应用一种或多种分类算法来分析数字化图像和吸收，透射率或反射光谱来分类植物胚胎的方法。这些方法通常是适用的，并强调基于客观标准获取和使用尽可能多的图像和吸收，透射率或反射光谱信息的重要性。本发明允许自动选择最适合进一步培养和排除那些被认为不太适合的胚胎。

18. 发明申请

US20070271628A1 Wuschel (WUS) Gene Homologs 审中-公开
公开(公告)号：US20070271628A1
公开(公告)日：2007-11-22
申请号：US11754389
申请日：2007-05-29
申请人： Keith Lowe , Rebecca Cahoon , Christopher Scelonge , Yumin Tao , William Gordon-Kamm , Wesley Bruce , Lisa Newman
发明人： Keith Lowe , Rebecca Cahoon , Christopher Scelonge , Yumin Tao , William Gordon-Kamm , Wesley Bruce , Lisa Newman
IPC分类号： A01H5/00 , C07H21/00 , C07H21/04 , C12N15/82 , C12N5/02
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.

19. 发明申请

US20070231864A1 Vanillin production 审中-公开
标题翻译：香草醛生产
公开(公告)号：US20070231864A1
公开(公告)日：2007-10-04
申请号：US11809646
申请日：2007-06-01
申请人： Daphna Havkin-Frenkel , Andrzej Podstolski
发明人： Daphna Havkin-Frenkel , Andrzej Podstolski
IPC分类号： C12N5/14 , A01H5/00 , C12P1/04
CPC分类号： C12N9/0077 , A01H4/001 , C12N5/04 , C12N9/00 , C12N9/0006 , C12N15/8207 , C12N15/8243 , C12P7/24
摘要： Novel compositions and methods for improving vanillin production in cultured Vanilla planifolia and in intact plants are provided. Transgenic cells and plants having improved vanillin production are also provided.
摘要翻译：提供了用于改善培养的香草和完整植物中的香草醛生产的新型组合物和方法。还提供了具有改善的香草醛生产的转基因细胞和植物。

20. 发明申请

US20070143881A1 Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange 审中-公开
标题翻译：提高位点特异性多核苷酸交换效率的方法和组合
公开(公告)号：US20070143881A1
公开(公告)日：2007-06-21
申请号：US11427947
申请日：2006-06-30
申请人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu
发明人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu
IPC分类号： A01H1/00
CPC分类号： C12N15/8213 , C12N15/8205 , C12N15/8207
摘要： Methods and compositions using a site-specific integration system are combined with methods and compositions which deliver compositions via microinjection directly to the embryo sac of a plant. The methods allow for various components of the site-specific recombination system to be introduced into the cellular environment of the embryo sac a composition comprising at least one component of the site-specific recombination system is injected into an embryo sac, providing improved efficiency of expression, recombination, integration, exchange, excision and/or inversion of a polynucleotide of interest. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and tissues, plant parts, and/or plants produced therefrom. Cells, egg cells, zygotes, embryos, endosperm, tissues, seeds, and/or plants produced by the methods and comprising the polynucleotide(s) of interest are also provided.
摘要翻译：使用位点特异性整合系统的方法和组合物与通过显微注射将组合物直接递送至植物胚囊的方法和组合物组合。所述方法允许将位点特异性重组系统的各种组分引入胚囊的细胞环境中，将包含位点特异性重组系统的至少一个组分的组合物注入胚囊，提供更好的表达效率，重组，整合，交换，切除和/或转化感兴趣的多核苷酸。感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳以及由其产生的组织，植物部分和/或植物的基因组中。还提供了通过方法产生并包含感兴趣的多核苷酸的细胞，卵细胞，合子，胚胎，胚乳，组织，种子和/或植物。

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