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1. 发明授权

US5736369A Method for producing transgenic cereal plants 失效
标题翻译：转基因谷物植物的生产方法
公开(公告)号：US5736369A
公开(公告)日：1998-04-07
申请号：US483091
申请日：1995-06-07
申请人： Benjamin A. Bowen , Keith Lowe , Margit C. Ross , Gary A. Sandahl , Dwight T. Tomes , William J. Gordon-Kamm , David D. Songstad
发明人： Benjamin A. Bowen , Keith Lowe , Margit C. Ross , Gary A. Sandahl , Dwight T. Tomes , William J. Gordon-Kamm , David D. Songstad
IPC分类号： C12N15/82 , C12N15/00 , A01H4/00 , C12N15/05
CPC分类号： C12N15/8207 , Y10S47/01
摘要： To obtain a transgenic cereal plant which is stably transformed, an exposed cereal meristem is subjected to biolistic bombardment in order to target non-differentiated meristem cells for transformation. Immature embryos at the early proembryo, mid proembryo, late proembryo, transitional or early coleoptilar stage are harvested for biolistic bombardment. The meristem tissue or cells fated to contribute to the meristem then are manipulated in order to enlarge transgenic sectors, either through selection and/or through effecting a proliferation from the tissue of shoots or multiple meristems per se. The shoot population thus obtained then is screened, by means of a nonlethal enrichment assay, to identify either chimeric sectors that will contribute to germline transmission, or non-sectored, L2 periclinal chimeras that will by definition transmit to progeny. Increased time in culture, under selection, enhances the prospects for sectoral-to-periclinal conversions, and also selects for L1-to-L2 conversions which, through a shift in position, ultimately contribute to the germline. Transgenic sectors also are stabilized during the step of tillering.
摘要翻译：为了获得稳定转化的转基因谷物植物，将暴露的谷物分生组织进行生物射弹轰击，以靶向未分化的分生组织转化。收获早期胚胎，中胚胎，晚期胚胎，过渡期或早期胚胎发育阶段的未成熟胚胎进行生物射弹轰击。为了通过选择和/或通过实现来自组织或多个分生组织本身的增殖来扩大转基因部分，操纵分生组织或细胞，以促进分生组织。然后通过非致死浓缩测定筛选由此获得的芽种群，以鉴定将有助于种系传播的嵌合区段或将通过定义向子代传播的非分区的L2周边嵌合体。增加的文化时间在不断的选择下，增加了部门间的转化前景，并选择了L1到L2的转换，通过转变，最终有助于种系。转基因部门在分蘖过程中也趋于稳定。

2. 发明授权

US08124411B2 Methods for producing transgenic plants 有权
标题翻译：生产转基因植物的方法
公开(公告)号：US08124411B2
公开(公告)日：2012-02-28
申请号：US11848554
申请日：2007-08-31
申请人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
IPC分类号： C12N5/04 , C12N5/10
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
摘要翻译：证明了在单个容器中生产稳定转化的玉米植物的方法。这种方法允许转换过程的自动化，并减少与传统植物组织培养系统相关的劳动力，材料和人体工学成本。

3. 发明申请

US20080118981A1 METHODS FOR PRODUCING TRANSGENIC PLANTS 有权
标题翻译：生产转基因植物的方法
公开(公告)号：US20080118981A1
公开(公告)日：2008-05-22
申请号：US11848554
申请日：2007-08-31
申请人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
IPC分类号： C12N5/04 , C12N1/20 , C12N5/02
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
摘要翻译：证明了在单个容器中生产稳定转化的玉米植物的方法。这种方法允许转换过程的自动化，并减少与传统植物组织培养系统相关的劳动力，材料和人体工学成本。

4. 发明授权

US08513016B2 Methods for producing transgenic plants 有权
公开(公告)号：US08513016B2
公开(公告)日：2013-08-20
申请号：US13355312
申请日：2012-01-20
申请人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
IPC分类号： C12N5/00
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

5. 发明申请

US20120180166A1 METHODS FOR PRODUCING TRANSGENIC PLANTS 有权
标题翻译：生产转基因植物的方法
公开(公告)号：US20120180166A1
公开(公告)日：2012-07-12
申请号：US13355312
申请日：2012-01-20
申请人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
IPC分类号： C12N15/82 , C12N5/04 , B02C19/00 , C12N1/32
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
摘要翻译：证明了在单个容器中生产稳定转化的玉米植物的方法。这种方法允许转换过程的自动化，并减少与传统植物组织培养系统相关的劳动力，材料和人体工学成本。

6. 发明申请

US20100235942A1 METHODS FOR CORN TRANSFORMATION 有权
标题翻译： CORN转换方法
公开(公告)号：US20100235942A1
公开(公告)日：2010-09-16
申请号：US12729072
申请日：2010-03-22
申请人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
发明人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
IPC分类号： A01H5/00 , A01H4/00 , C12N15/82
CPC分类号： C12N15/8207 , C12N15/8205 , C12N15/8206 , C12N15/8209
摘要： The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.
摘要翻译：本发明涉及一种用于产生转化玉米植物的新型转化系统。特别地，本发明涉及一种在高温下的快速选择系统，其允许更快和更有效的转化。

7. 发明授权

US07205459B1 Transformable inbred corn line LIZL5 and methods for use thereof 有权
标题翻译：可变形近交玉米线LIZL5及其使用方法
公开(公告)号：US07205459B1
公开(公告)日：2007-04-17
申请号：US09606808
申请日：2000-06-28
申请人： James R. Larkins , David D. Songstad , William L. Petersen , Hongyi Zhang , Michael T. Mann , T. Michael Spencer , Nancy G. Willetts
发明人： James R. Larkins , David D. Songstad , William L. Petersen , Hongyi Zhang , Michael T. Mann , T. Michael Spencer , Nancy G. Willetts
IPC分类号： A01H5/10 , A01H5/00 , A01H1/00 , C12N5/14 , C12N15/82 , C12N5/04
CPC分类号： A01H5/10 , A01H4/005 , C12N15/8205 , C12N15/8207
摘要： According to the invention, there is provided an inbred corn plant designated LIZL5. This invention thus relates to the plants, seeds and tissue cultures of the inbred corn plant LIZL5, and to methods for producing a corn plant produced by crossing the inbred corn plant LIZL5 with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing the inbred plant LIZL5 with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plant LIZL5, and also to the SSR and genetic isozyme typing profiles of inbred corn plant LIZL5. This invention further relates to methods of transforming the inbred LIZL5 and cells thereof, transformed plants produced by these methods, progeny transgenic plants, and seed derived therefrom.
摘要翻译：根据本发明，提供了称为LIZL5的近交玉米植物。因此，本发明涉及近交玉米植物LIZL5的植物，种子和组织培养物，以及通过将近交玉米植物LIZL5与其他玉米植物如另一近交系交叉产生的玉米植物的生产方法。本发明还涉及通过将近交植物LIZL5与另一玉米植物（例如另一近交系）杂交并与相关物种杂交而产生的玉米种子和植物。本发明还涉及近交玉米植物LIZL5的近交和杂交遗传互补，以及近交玉米植物LIZL5的SSR和遗传同工酶分型。本发明还涉及转化近交LIZL5及其细胞，通过这些方法产生的转化植物，后代转基因植物和从其衍生的种子的方法。

8. 发明授权

US08759609B2 Methods for corn transformation 有权
标题翻译：玉米转化方法
公开(公告)号：US08759609B2
公开(公告)日：2014-06-24
申请号：US12729072
申请日：2010-03-22
申请人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
发明人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
IPC分类号： C07K14/415 , A01H5/10 , A01H1/02
CPC分类号： C12N15/8207 , C12N15/8205 , C12N15/8206 , C12N15/8209
摘要： The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.
摘要翻译：本发明涉及一种用于产生转化玉米植物的新型转化系统。特别地，本发明涉及一种在高温下的快速选择系统，其允许更快和更有效的转化。

9. 发明授权

US07682829B2 Methods for corn transformation 有权
标题翻译：玉米转化方法
公开(公告)号：US07682829B2
公开(公告)日：2010-03-23
申请号：US10709826
申请日：2004-06-01
申请人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
发明人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
IPC分类号： C12N15/84 , C12N5/00 , C12N1/20 , C12N15/82
CPC分类号： C12N15/8207 , C12N15/8205 , C12N15/8206 , C12N15/8209
摘要： The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.
摘要翻译：本发明涉及一种用于产生转化玉米植物的新型转化系统。特别地，本发明涉及一种在高温下的快速选择系统，其允许更快和更有效的转化。

10. 发明授权

US5717129A Methods for maintaining sterility in plants 失效
标题翻译：在植物中维持不育的方法
公开(公告)号：US5717129A
公开(公告)日：1998-02-10
申请号：US389389
申请日：1995-02-16
申请人： David D. Songstad , Steven J. Corak , Dorothy A. Pierce , Marc Albertsen
发明人： David D. Songstad , Steven J. Corak , Dorothy A. Pierce , Marc Albertsen
IPC分类号： A01H1/00 , A01H1/06 , A01H5/00 , C12N5/10 , C12N9/00 , C12N9/10 , C12N15/09 , C12N15/82 , C12R1/91 , A01H4/00 , C12N15/00 , C12N15/63
CPC分类号： C12N15/829 , A01H1/06 , C12N15/8289 , Y10S47/01
摘要： A method of maintaining sterility in plants is disclosed. The method comprises genetic transformation of parental plant lines with a sterility gene that is genetically linked to a resistance gene which confers resistance to a selective agent, increasing the transgenic parental line and coating the seed of the increased transgenic parental line with a composition comprising the selective agent to which the resistance gene confers resistance. The resistance gene can be under the control of an inducible gene, or, alternatively, under the control of constitutive or tissue specific, including seed-specific, promoters.
摘要翻译：公开了在植物中维持不育的方法。该方法包括亲本植物系的遗传转化，所述无性基因与抗性基因遗传连锁，所述抗性基因赋予对选择性试剂的抗性，增加转基因亲代株系并用包含选择性的组合物包含增加的转基因亲本系的种子抗性基因赋予抗性的药剂。抗性基因可以在可诱导基因的控制下，或者在组成型或组织特异性的控制下，包括种子特异性启动子。

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