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11. 发明授权

US08802448B2 Mixed mode ligands 有权
标题翻译：混合模式配体
公开(公告)号：US08802448B2
公开(公告)日：2014-08-12
申请号：US13555484
申请日：2012-07-23
申请人： Hassan Ait-Haddou , Frank Onyemauwa , Jonathan Haigh , Samuel Nochumson
发明人： Hassan Ait-Haddou , Frank Onyemauwa , Jonathan Haigh , Samuel Nochumson
IPC分类号： G01N33/547 , G01N33/538 , G01N33/53
CPC分类号： B01J20/3248 , B01D15/166 , B01D15/3847 , B01J20/24 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3253 , B01J20/3255 , B01J20/3285 , C07K1/165 , Y02P20/582
摘要： Substrates comprising a solid support, a ligand, and a linker comprising at least one C, O, N, or S atom covalently connecting the solid support to the ligand, are disclosed, along with methods of using and making the substrates, and devices including the substrates.
摘要翻译：公开了包含固体支持物，配体和包含至少一个共价连接固体支持物与配体的C，O，N或S原子的底物，以及使用和制备底物的方法，以及包括底物。

12. 发明申请

US20130109102A1 LIQUID CHROMATOGRAPHY SYSTEM AND METHOD FOR PROTEIN SEPARATION AND PURIFICATION 审中-公开
标题翻译：液相色谱系统和蛋白质分离和纯化方法
公开(公告)号：US20130109102A1
公开(公告)日：2013-05-02
申请号：US13237326
申请日：2011-09-20
申请人： HIROYUKI YABE , TOMOYUKI MIYABAYASHI
发明人： MICHAEL LI , HIROYUKI YABE , TOMOYUKI MIYABAYASHI
IPC分类号： G01N31/16
CPC分类号： G01N31/16 , B01D15/166 , B01D15/361 , C07K1/18 , G01N30/34 , Y10T436/116664
摘要： A system and method for protein separation and purification includes a mixing loop, a primary pump having an inlet in communication with a supply of an acid or an alkali and an outlet in communication with the mixing loop. A concentrate pump has an inlet in communication with a supply of an acid or an alkali and an outlet in communication with the mixing loop. A liquid chromatography column is in communication with the mixing loop and is packed with an ion exchange resin and loaded with a protein solution containing a first protein having a first isoelectric point and a second protein having a second isoelectric point. A process control feedback loop in communication with the mixing loop includes an inline process pH analyzer so that a pH of a titration solution in the mixing loop may be detected and adjusted based on the detected pH. The titration solution is provided in the mixing loop by the primary and concentrate pumps and the pH of the titration solution in the mixing loop is adjusted by the feedback loop based on the pH detected by the inline process pH analyzer to provide a generally linear pH gradient to the chromatography column so that the first and second proteins are separately eluded from the liquid chromatography column.
摘要翻译：用于蛋白质分离和纯化的系统和方法包括混合回路，具有与酸或碱供应源连通的入口和与混合回路连通的出口的初级泵。浓缩泵具有与酸或碱供应源连接的入口和与混合回路连通的出口。液相色谱柱与混合回路连通，并填充有离子交换树脂，并装载含有具有第一等电点的第一蛋白质和具有第二等电点的第二蛋白质的蛋白质溶液。与混合回路连通的过程控制反馈回路包括在线过程pH分析仪，使得可以基于检测到的pH来检测和调整混合回路中的滴定溶液的pH。滴定溶液通过初级浓缩泵和浓缩泵在混合回路中提供，并且基于通过在线工艺pH分析仪检测的pH通过反馈回路调节混合回路中的滴定溶液的pH，以提供通常的线性pH梯度到色谱柱，使得第一和第二种蛋白质分离地从液相色谱柱中除去。

13. 发明申请

US20130029365A1 MULTI-DIMENSIONAL CHROMATOGRAPHIC METHODS FOR SEPARATING N-GLYCANS 有权
标题翻译：用于分离N-GLYCANS的多维色谱方法
公开(公告)号：US20130029365A1
公开(公告)日：2013-01-31
申请号：US13630594
申请日：2012-09-28
申请人： MOMENTA PHARMACEUTICALS, INC.
发明人： Ian Christopher Parsons , Ting Zheng , Nur Sibel Gunay , Carlos J. Bosques
IPC分类号： G01N30/02 , G01N30/00
CPC分类号： G01N33/6803 , B01D15/166 , B01D15/1864 , B01D15/1878 , B01D15/325 , B01D15/363 , B01D15/426 , C07K1/36 , G01N1/34 , G01N30/463 , G01N33/6854 , G01N2400/00 , G01N2440/38 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , Y10T436/145555
摘要： A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique.
摘要翻译：用于分离N-聚糖的多维色谱方法。该方法包括提供包含至少一个带负电荷的N-聚糖的聚糖制剂。然后通过阴离子交换层析和至少一种二级色谱技术分离聚糖制备物。

14. 发明申请

US20120055876A1 METHOD AND INSTALLATION FOR REGULATING THE MODIFIER LEVEL IN CHROMATOGRAPHY OR SUPERCRITICAL EXTRACTION WITH RECYCLING 有权
标题翻译：用于调节色谱法或超临界萃取中的改性剂水平的方法和装置
公开(公告)号：US20120055876A1
公开(公告)日：2012-03-08
申请号：US13294565
申请日：2011-11-11
申请人： Mohamed Shaimi
发明人： Mohamed Shaimi
IPC分类号： B01D15/16 , B01D11/00 , B01D15/40
CPC分类号： B01D15/40 , B01D15/166 , B01D15/1814
摘要： A chromatography or supercritical extraction method is disclosed, in which the eluent comprises a mixture of a fluid and a modifier and in which the fluid is recycled. One exemplary method comprises an operation consisting in determining at least one quantity linked to the level of modifier that is mixed with the recycled fluid and, if necessary, a correction operation in order to limit variations in the level of modifier in the eluent at the inlet of the column or the extractor. The disclosure also relates to a chromatography or extraction installation.
摘要翻译：公开了一种色谱法或超临界萃取方法，其中洗脱液包括流体和改性剂的混合物，其中流体被再循环。一个示例性方法包括确定与修复剂水平相关联的至少一个量的操作，所述量与再循环流体混合，并且如果需要，进行校正操作，以限制入口处洗脱液中改性剂水平的变化的列或提取器。本公开还涉及色谱或提取装置。

15. 发明申请

US20120022234A1 METHOD FOR THE PURIFICATION OF ALBUMIN CONJUGATES 审中-公开
标题翻译：氧化铝结合物的纯化方法
公开(公告)号：US20120022234A1
公开(公告)日：2012-01-26
申请号：US12885036
申请日：2010-09-17
申请人： Nathalie Bousquet-Gagnon , Omar Quraishi , Dominique P. Bridon
发明人： Nathalie Bousquet-Gagnon , Omar Quraishi , Dominique P. Bridon
IPC分类号： C07K1/20
CPC分类号： C07K1/303 , A61K47/643 , B01D15/166 , B01D15/327 , B01D15/426 , C07K1/20 , C07K14/76 , C07K14/765
摘要： The present invention relates to a method for separating albumin conjugate from unconjugated albumin in a solution comprising albumin conjugate and unconjugated albumin by loading the solution onto a hydrophobic support equilibrated in aqueous buffer having a high salt content; applying to the support a gradient of decreasing salt concentration; and collecting the eluted albumin conjugate.
摘要翻译：本发明涉及一种通过将溶液加载到具有高盐含量的水性缓冲液中平衡的疏水性载体上的将白蛋白结合物与未结合的白蛋白分离在包含白蛋白结合物和未结合的白蛋白的溶液中的方法; 向支持体施用降低盐浓度的梯度; 并收集洗脱的白蛋白缀合物。

16. 发明申请

US20120006750A1 Method of manufacturing substances by supercritical fluid chromatography 有权
公开(公告)号：US20120006750A1
公开(公告)日：2012-01-12
申请号：US13138672
申请日：2009-10-22
申请人： Kenichiro Miyazawa , Takeshi Ishiguro
发明人： Kenichiro Miyazawa , Takeshi Ishiguro
IPC分类号： B01D15/08
CPC分类号： G01N30/34 , B01D15/166 , B01D15/40 , G01N30/84
摘要： Provided is a method of manufacturing target substances with use of supercritical fluid chromatography, by which the following are achieved: solution of a problem at the time of sequential injections of samples containing the target substances; an increase of a treatment amount of separation per unit time; and improvement of efficiency in separation. The method includes the steps of: injecting the sample containing the target substances into a mobile phase; and returning composition of the mobile phase to a pre-change state after changing the composition of the mobile phase. The step of returning the composition of the mobile phase to the pre-change state after changing the composition of the mobile phase is performed during a period of time from detection of a peak of one of the target substances which is eluted latest from a column among the target substances separated by the supercritical fluid chromatography apparatus to injection of the next sample, whereby the problem is solved.

17. 发明申请

US20110155668A1 SEPARATION MEDIUM FOR CHROMATOGRAPHY OF VARIOUS BIOMOLECULES 审中-公开
标题翻译：分离介质用于各种生物分子的色谱分析
公开(公告)号：US20110155668A1
公开(公告)日：2011-06-30
申请号：US13002878
申请日：2009-06-04
申请人： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman
发明人： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman
IPC分类号： B01D15/36 , B01J20/22 , B01J20/26 , B01J20/24
CPC分类号： B01D15/3828 , B01D15/166 , B01D15/36 , B01J20/28092 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3293 , C07K1/16 , C07K1/22 , C07K16/00
摘要： The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.
摘要翻译：本发明涉及一种分离介质，其包括具有带电配位体的多孔材料的内核和包含带有带电配位体的多孔材料的外盖，其中内芯中配体的电荷与盖中配体的电荷。本发明还涉及一种用于生物分子分离的方法，包括将样品施加到上述分离介质中，其中通过从介质的电荷排斥来防止大分子进入介质，并且小分子被捕获在内核中。

18. 发明授权

US07921696B2 Liquid chromatograph device 有权
标题翻译：液相色谱仪
公开(公告)号：US07921696B2
公开(公告)日：2011-04-12
申请号：US12037111
申请日：2008-02-26
申请人： Kunihiko Takao , Hironori Kaji , Masahito Ito
发明人： Kunihiko Takao , Hironori Kaji , Masahito Ito
IPC分类号： G01N30/00 , B01D15/08
CPC分类号： G01N30/32 , B01D15/14 , B01D15/166 , F04B11/0058 , F04B11/0083 , F04B13/00 , F04B13/02 , F04B53/06 , G01N2030/326
摘要： This liquid feeding system has the first and second pumps each of which is provided with a plunger. Liquid sucked through each of the pump suction ports is discharged out of the discharging port through the first pump and/or second pump. Under the starting operation mode, the second pump is stopped and only the first pump is operated. When the discharging pressure at the discharging port reaches up to a predetermined value, the starting operation mode is changed over to the normal operation mode. Under the normal operation mode, the first pump is stopped and only the second pump is operated.
摘要翻译：该液体供给系统具有第一和第二泵，每个泵设有柱塞。通过每个泵吸入口吸入的液体通过第一泵和/或第二泵排出排出口。在启动运行模式下，第二个泵停止运转，只有第一个泵运转。当排出口的排出压力达到预定值时，启动操作模式转换到正常操作模式。在正常运行模式下，第一个泵停止运转，只有第二个泵运转。

19. 发明申请

US20100176043A1 MOBILE PHASE DILUTION SCHEME FOR ENHANCED CHROMATOGRAPHY 有权
标题翻译：用于增强色谱的移动相位稀释方案
公开(公告)号：US20100176043A1
公开(公告)日：2010-07-15
申请号：US12726919
申请日：2010-03-18
申请人： Thomas E. Wheat , Charles H. Phoebe , Mark K. Baynham , Uwe Dieter Neue , Raymond P. Fisk , Richard C. Turner
发明人： Thomas E. Wheat , Charles H. Phoebe , Mark K. Baynham , Uwe Dieter Neue , Raymond P. Fisk , Richard C. Turner
IPC分类号： B01D15/22
CPC分类号： G01N30/34 , B01D15/166 , B01D15/22 , G01N30/16 , G01N2030/347
摘要： A method and device for increasing the loading capacity of a chromatography column through dilution of a mobile phase at the head of the column. A strong mobile phase is provided for dissolving a sample. A sample is injected into the strong mobile phase, and subsequently diluted with a weak mobile phase. The resulting sample-containing weak mobile phase is passed through a chromatography column for retention and separation of the components of the sample.
摘要翻译：一种用于通过稀释柱顶部的流动相来增加色谱柱的负载能力的方法和装置。提供强的流动相来溶解样品。将样品注入强流动相，随后用弱流动相稀释。所得到的含有样品的弱流动相通过色谱柱，用于保留和分离样品组分。

20. 发明申请

US20100065495A1 APPARATUS AND METHODS FOR PREPARATIVE LIQUID CHROMATOGRAPHY 有权
标题翻译：用于制备液相色谱的装置和方法
公开(公告)号：US20100065495A1
公开(公告)日：2010-03-18
申请号：US12555032
申请日：2009-09-08
申请人： Joshua A. Shreve , Michael R. Jackson
发明人： Joshua A. Shreve , Michael R. Jackson
IPC分类号： B01D15/42 , B01D15/08 , B01D15/18
CPC分类号： B01D15/18 , B01D15/166 , G01N30/34
摘要： A method of liquid chromatography includes providing one or more solvent reservoirs, providing a solvent pump, drawing one or more solvents into the pump in response to a pressure drop that promotes outgassing of the solvents, and dispersing outgassed bubbles into smaller bubbles to promote re-dissolution of the gas. A liquid-chromatography apparatus includes at least two solvent reservoirs, a pump, at least one bubble-dispersing unit that receives a pressurized flow of proportioned solvents from the pump, and a control unit. The control unit includes a processor and a memory that stores instructions; the control unit controls proportioning of solvents to obtain a preselected solvent composition, and pumping at flow rates to support preparative-scale or process-scale liquid chromatography.
摘要翻译：液相色谱法包括提供一个或多个溶剂储存器，提供溶剂泵，响应于促进溶剂除气的压降而将一种或多种溶剂吸入泵中，以及将脱气气泡分散成较小的气泡，溶解气体。液相色谱装置包括至少两个溶剂储存器，泵，至少一个气泡分散单元，其从泵接收加压的成比例溶剂流，以及控制单元。控制单元包括处理器和存储指令的存储器; 控制单元控制溶剂的比例以获得预选的溶剂组合物，并以流速泵送以支持制备规模或过程规模的液相色谱。

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