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    • 11. 发明授权
    • Expression library screen by prenylation of expressed proteins
    • 通过表达蛋白质的异戊烯化表达文库筛选
    • US5932432A
    • 1999-08-03
    • US785795
    • 1997-01-21
    • Dring N. CrowellBrenda BiermannStephen Randall
    • Dring N. CrowellBrenda BiermannStephen Randall
    • C12Q1/02C12Q1/48C12Q1/68G01N33/58
    • C12Q1/02C12Q1/48
    • Described is a novel in vitro method for obtaining and identifying proteins which, in their natural in vivo setting, are covalently modified after translation. To identify novel isoprenylated proteins for subsequent biochemical study, colony blots of a Glycine max cDNA expression library were �.sup.3 H! farnesyl labeled in vitro. Proteins identified by this screen contained several different carboxy-termini that conform to consensus farnesylation motifs. These proteins included known farnesylated proteins (DnaJ homologs) and several novel proteins, two of which contained 6 or more tandem repeats of a hexapeptide having the consensus sequence �E or G! �G or P!EK�P or K!K. Expression library screening by direct labeling can thus be adapted to recover and identify isoprenylated proteins as well as proteins with other post-translational modifications. This identification and recovery further enables the recovery of transformants containing DNA encoding the proteins, as well as the raising of antibodies to the proteins.
    • 描述了一种用于获得和鉴定蛋白质的新颖的体外方法,其在其天然的体内环境中在翻译后共价修饰。 为了鉴定新的异戊二烯基蛋白用于随后的生物化学研究,大肠杆菌cDNA表达文库的集落印迹在体外被标记为[3 H]法呢基。 通过该筛选鉴定的蛋白质包含符合共同法尼基化基序的若干不同羧基末端。 这些蛋白质包括已知的法呢基化蛋白质(DnaJ同系物)和几种新型蛋白质,其中两种含有6个或更多个具有共有序列[E或G] [G或P] EK [P或K] K的六肽的串联重复。 因此,通过直接标记的表达文库筛选可以适应于恢复和鉴定异戊二烯基化蛋白质以及具有其他翻译后修饰的蛋白质。 该鉴定和回收进一步使得能够回收含有编码蛋白质的DNA的转化体,以及提高对蛋白质的抗体。
    • 13. 发明授权
    • System and method for location-based interactive content
    • 基于位置的互动内容的系统和方法
    • US07450954B2
    • 2008-11-11
    • US11275939
    • 2006-02-06
    • Stephen Randall
    • Stephen Randall
    • H04Q7/20
    • H04L67/306H04L67/18H04M19/04
    • A system and method for providing and managing location-based interactive content, including messages sent to specific location-based screens via mobile communication devices or the Internet. The location-based screens are display devices connected to a central content server that may be a central website, by a first network that may be the Internet. Each location-based display device may be independently controlled by a communications management module at the central website. The communications management module includes software and hardware so that it may record a user's responses and may update any screen according to system policies.
    • 一种用于提供和管理基于位置的交互式内容的系统和方法,包括经由移动通信设备或因特网发送到特定位置的屏幕的消息。 基于位置的屏幕是通过可能是因特网的第一网络连接到可以是中央网站的中央内容服务器的显示设备。 每个基于位置的显示设备可以由中央网站上的通信管理模块独立地控制。 通信管理模块包括软件和硬件,使得其可以记录用户的响应,并且可以根据系统策略更新任何屏幕。
    • 16. 发明申请
    • System and method for setup of toner concentration target for a toner concentration sensor
    • 用于设置调色剂浓度传感器的调色剂浓度目标的系统和方法
    • US20060153581A1
    • 2006-07-13
    • US11032935
    • 2005-01-11
    • Song-Feng MoWendy AptonJennifer WagnerStephen RandallPatricio MedinaPatrick WalkerRichard Aman
    • Song-Feng MoWendy AptonJennifer WagnerStephen RandallPatricio MedinaPatrick WalkerRichard Aman
    • G03G15/00
    • G03G15/5041
    • A method by which the target of a toner concentration (TC) sensor can be set up based on the equivalent developability concept without using an expensive patch generator. First, use a black toner area coverage (BTAC) sensor to measure the relative reflectance (RR) of a 100% area coverage developed patch (ROS generated) at a series of development levels (Vem or Vdev) to generate a pseudo development curve and estimate VdO in real-time (VdO is the Vdev level that starts to develop toner onto the photoreceptor belt.) Then, add a nominal delta Vdev to VdO to get the development level (Vdev) needed to generate 100% area coverage gray patch. Finally, using a BTAC sensor to measure the RR level of a 100% area coverage (AC) gray patch, move the target of the TC sensor, and converge the sensor's reading to the sensor's target continuously until the RR level of the gray patch hits the RR target (predefined) within the tolerance range. Thus, the target of the TC sensor is set for the xerographic system automatically.
    • 可以在不使用昂贵的贴片发生器的情况下,基于等效显影性概念设置调色剂浓度(TC)传感器的目标的方法。 首先,使用黑色调色剂区域覆盖(BTAC)传感器来测量在一系列开发水平(V SUB)或V(V)下的100%区域覆盖开发贴片(ROS生成)的相对反射率(RR) )以生成伪显影曲线并且实时地估计V OUT(V OUT)是V >水平,开始在感光带上显影调色剂。)然后,向V DO 添加额定的ΔV以获得显影水平(V < / SUB>)需要生成100%的区域覆盖灰度补丁。 最后,使用BTAC传感器测量100%区域覆盖(AC)灰色块的RR电平,移动TC传感器的目标,并将传感器的读数连续收敛到传感器的目标位置,直到灰色片段的RR电平达到 RR目标(预定义)在公差范围内。 因此,TC传感器的目标将自动设置为静电复印系统。
    • 17. 发明申请
    • System and Method for Interactive Marketing
    • 互动营销系统与方法
    • US20070294096A1
    • 2007-12-20
    • US11666628
    • 2005-11-04
    • Stephen RandallStephen AnJeffrey Potter
    • Stephen RandallStephen AnJeffrey Potter
    • G06Q99/00G09G5/00H04K1/00H04L12/28H04Q7/20
    • G06Q30/0255G06Q20/322G06Q30/00G06Q30/0267H04L63/10H04W4/02
    • A system and method of interactive, location-based presentation and advertising that enables users with wireless network addressable communications devices to control multi-media content on network addressable screens and enables marketers to track, monitor and respond to users interactions in real time. A Proxy Gateway directs a network addressable client PC, connected to a digital display, to serve local content or pull content from one or more web servers. A wireless, network addressable device, typically a mobile phone, is used to communicate commands to the physically remote Proxy Gateway. The Proxy Gateway bridges between the communications device and the display by interpreting the commands from the communications device, forwarding them to the client PC which may pull content from the appropriate web server, or display native message content.
    • 基于交互式,基于位置的呈现和广告的系统和方法,使具有无线网络可寻址通信设备的用户能够控制网络可寻址屏幕上的多媒体内容,并使营销人员能够实时跟踪,监控和响应用户交互。 代理网关指示连接到数字显示器的网络可寻址客户端PC,以服务本地内容或从一个或多个Web服务器提取内容。 无线网络可寻址设备(通常为移动电话)用于向物理上远程的代理网关传送命令。 代理网关通过解释来自通信设备的命令,将它们转发到可以从适当的web服务器提取内容或显示本机消息内容的客户端PC来在通信设备和显示器之间桥接。
    • 18. 发明申请
    • System and Method for Location-based Interactive Content
    • 基于位置的互动内容的系统和方法
    • US20060179127A1
    • 2006-08-10
    • US11275939
    • 2006-02-06
    • Stephen Randall
    • Stephen Randall
    • G06F15/16G08B5/22H04Q1/30
    • H04L67/306H04L67/18H04M19/04
    • A system and method for providing and managing location-based interactive content, including messages sent to specific location-based screens via mobile communication devices or the Internet. The location-based screens are display devices connected to a central content server that may be a central website, by a first network that may be the Internet. Each location-based display device may be independently controlled by a communications management module at the central website. The communications management module includes software and hardware so that it may record a user's responses and may update any screen according to system policies.
    • 一种用于提供和管理基于位置的交互式内容的系统和方法,包括经由移动通信设备或因特网发送到特定位置的屏幕的消息。 基于位置的屏幕是通过可能是因特网的第一网络连接到可以是中央网站的中央内容服务器的显示设备。 每个基于位置的显示设备可以由中央网站上的通信管理模块独立地控制。 通信管理模块包括软件和硬件,使得其可以记录用户的响应,并且可以根据系统策略更新任何屏幕。