专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5932432A Expression library screen by prenylation of expressed proteins 失效
标题翻译：通过表达蛋白质的异戊烯化表达文库筛选
公开(公告)号：US5932432A
公开(公告)日：1999-08-03
申请号：US785795
申请日：1997-01-21
申请人： Dring N. Crowell , Brenda Biermann , Stephen Randall
发明人： Dring N. Crowell , Brenda Biermann , Stephen Randall
IPC分类号： C12Q1/02 , C12Q1/48 , C12Q1/68 , G01N33/58
CPC分类号： C12Q1/02 , C12Q1/48
摘要： Described is a novel in vitro method for obtaining and identifying proteins which, in their natural in vivo setting, are covalently modified after translation. To identify novel isoprenylated proteins for subsequent biochemical study, colony blots of a Glycine max cDNA expression library were �.sup.3 H! farnesyl labeled in vitro. Proteins identified by this screen contained several different carboxy-termini that conform to consensus farnesylation motifs. These proteins included known farnesylated proteins (DnaJ homologs) and several novel proteins, two of which contained 6 or more tandem repeats of a hexapeptide having the consensus sequence �E or G! �G or P!EK�P or K!K. Expression library screening by direct labeling can thus be adapted to recover and identify isoprenylated proteins as well as proteins with other post-translational modifications. This identification and recovery further enables the recovery of transformants containing DNA encoding the proteins, as well as the raising of antibodies to the proteins.
摘要翻译：描述了一种用于获得和鉴定蛋白质的新颖的体外方法，其在其天然的体内环境中在翻译后共价修饰。为了鉴定新的异戊二烯基蛋白用于随后的生物化学研究，大肠杆菌cDNA表达文库的集落印迹在体外被标记为[3 H]法呢基。通过该筛选鉴定的蛋白质包含符合共同法尼基化基序的若干不同羧基末端。这些蛋白质包括已知的法呢基化蛋白质（DnaJ同系物）和几种新型蛋白质，其中两种含有6个或更多个具有共有序列[E或G] [G或P] EK [P或K] K的六肽的串联重复。因此，通过直接标记的表达文库筛选可以适应于恢复和鉴定异戊二烯基化蛋白质以及具有其他翻译后修饰的蛋白质。该鉴定和回收进一步使得能够回收含有编码蛋白质的DNA的转化体，以及提高对蛋白质的抗体。

IPRDB

热门服务

关于我们

友情链接

联系方式