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11. 发明申请

US20130230516A1 TARGETED CRYPTOSPORIDIUM BIOCIDES 有权
标题翻译：有针对性的CRYPTOSPORIDIUM BIOCIDES
公开(公告)号：US20130230516A1
公开(公告)日：2013-09-05
申请号：US13760448
申请日：2013-02-06
申请人： Michael Imboden , Michael Riggs , Deborah A. Schaefer , Jane Homan , Robert D. Bremel
发明人： Michael Imboden , Michael Riggs , Deborah A. Schaefer , Jane Homan , Robert D. Bremel
IPC分类号： C07K16/20
CPC分类号： C07K16/20 , A61K2039/505 , C07K14/4703 , C07K2317/12 , C07K2317/56 , C07K2317/76 , C07K2319/00 , C07K2319/01 , C12N9/18
摘要： The present invention relates to fusion proteins comprising a microorganism targeting molecule (e.g., immunoglobulin) and a biocide. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in diverse fields.
摘要翻译：本发明涉及包含微生物靶向分子（例如免疫球蛋白）和杀生物剂的融合蛋白。本发明还涉及在不同领域中使用包含微生物靶向分子和杀生物剂的融合蛋白的治疗和预防方法。

12. 发明授权

US08222188B2 Antibody libraries 有权
标题翻译：抗体库
公开(公告)号：US08222188B2
公开(公告)日：2012-07-17
申请号：US12493851
申请日：2009-06-29
申请人： Robert D. Bremel , Kurt Eakle , Michael Imboden
发明人： Robert D. Bremel , Kurt Eakle , Michael Imboden
IPC分类号： C40B40/02 , C12Q1/70
CPC分类号： C07K16/00 , C07K2317/21 , C12N2799/027
摘要： The present invention relates to the production of antibody libraries. In particular, the present invention relates to the use of integrating retroviral vectors to generate libraries comprising a plurality of combinations of antibody light chains and heavy chains. The present invention thus provides improved methods of generating and screening antibody libraries comprising large numbers of unique antibodies.
摘要翻译：本发明涉及抗体文库的生产。特别地，本发明涉及整合逆转录病毒载体以产生包含抗体轻链和重链的多种组合的文库的用途。因此，本发明提供了产生和筛选包含大量独特抗体的抗体文库的改进方法。

13. 发明授权

US07332333B2 Host cells containing multiple integrating vectors 有权
标题翻译：含有多个整合载体的宿主细胞
公开(公告)号：US07332333B2
公开(公告)日：2008-02-19
申请号：US11018895
申请日：2004-12-21
申请人： Robert D. Bremel , Linda U. Miller , Gregory T. Bleck
发明人： Robert D. Bremel , Linda U. Miller , Gregory T. Bleck
IPC分类号： C12N15/867 , C12N5/10
CPC分类号： C12N15/86 , C07K14/005 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N2510/02 , C12N2730/10122 , C12N2740/13023 , C12N2740/13043 , C12N2740/13051 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12P21/02
摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.
摘要翻译：本发明涉及宿主细胞中蛋白质的制备，更具体地涉及含有整合载体的多个整合拷贝的宿主细胞。用于本发明的合适的整合载体包括逆转录病毒载体，慢病毒载体，转座子载体和腺相关病毒载体。提供了其中通过以高倍数感染使用整合载体制备宿主细胞的方法。宿主细胞可用于生产药物蛋白质，用于筛选测定的蛋白质变体，以及直接用于高通量筛选。

14. 发明授权

US5010011A Bovine placental lactogen gene 失效
标题翻译：牛胎盘乳腺基因
公开(公告)号：US5010011A
公开(公告)日：1991-04-23
申请号：US184901
申请日：1988-04-21
申请人： Linda A. Schuler , Jack Gorski , Walter L. Hurley , Robert D. Bremel , Fritz M. Rottman
发明人： Linda A. Schuler , Jack Gorski , Walter L. Hurley , Robert D. Bremel , Fritz M. Rottman
IPC分类号： C07K14/575 , C12N15/12
CPC分类号： C07K14/57518 , C07K14/57545
摘要： Portions of the genetic sequence coding for bovine placental lactogen are isolated and a cDNA variant of a bovine placental lactogen gene is then formed and isolated. Upon cloning of the cDNA gene sequence and culturing of a resulting host, large quantities of bovine placental lactogen can be produced.
摘要翻译：分离编码牛胎盘乳糖的基因序列的部分，然后形成并分离牛胎盘乳基因基因的cDNA变体。在克隆cDNA基因序列并培养所得宿主时，可以生产大量的牛胎盘乳糖。

15. 发明授权

US07297536B2 Inducible protein expression system 失效
标题翻译：诱导蛋白表达系统
公开(公告)号：US07297536B2
公开(公告)日：2007-11-20
申请号：US10763976
申请日：2004-01-23
申请人： Jerome S. Harms , Gary A. Splitter , Kurt A. Eakle , Robert D. Bremel
发明人： Jerome S. Harms , Gary A. Splitter , Kurt A. Eakle , Robert D. Bremel
IPC分类号： C12N15/85 , C12N15/864 , C12N15/861 , C12N15/867 , C12N15/48 , C12N15/49 , A61K48/00
CPC分类号： C12N15/86 , C12N2740/14043 , C12N2830/00 , C12N2830/50 , C12N2830/60
摘要： Inducible gene expression systems and a method thereof. A first inducible gene expression system includes a first vector comprising at least one retroviral promoter and at least one factor to induce the retroviral promoter. At least one gene product is expressed in proportion to retroviral promoter induction. The method includes providing a first vector comprising at least one retroviral promoter and providing at least one factor corresponding to the retroviral promoter. The retroviral promoter is induced with the at least one factor. At least one protein is expressed based on the induction of the retroviral promoter. A second inducing expression system includes a first vector comprising at least one retroviral promoter, an inducer for the retroviral promoter, and at least one protein expressed in proportion to retroviral promoter induction.
摘要翻译：诱导型基因表达系统及其方法。第一诱导型基因表达系统包括第一载体，其包含至少一个逆转录病毒启动子和至少一个诱导逆转录病毒启动子的因子。至少一种基因产物与逆转录病毒启动子诱导成比例地表达。该方法包括提供包含至少一个逆转录病毒启动子并提供至少一个与逆转录病毒启动子相对应的因子的第一载体。用至少一个因子诱导逆转录病毒启动子。基于逆转录病毒启动子的诱导表达至少一种蛋白质。第二诱导表达系统包括第一载体，其包含至少一个逆转录病毒启动子，用于逆转录病毒启动子的诱导子，和至少一种与逆转录病毒启动子诱导成比例的蛋白质。

16. 发明申请

US20100111976A1 TARGETED BIOCIDES 审中-公开
标题翻译：目标生物
公开(公告)号：US20100111976A1
公开(公告)日：2010-05-06
申请号：US12566108
申请日：2009-09-24
申请人： Michael Imboden , Jane Homan , Robert D. Bremel
发明人： Michael Imboden , Jane Homan , Robert D. Bremel
IPC分类号： A61K39/395 , A01N37/18 , A01P1/00 , A01P15/00 , A61P31/00
CPC分类号： A01N63/04 , A01N63/00 , A01N63/02 , A01N65/00 , C07K16/00 , C07K16/20 , C07K2317/52 , C07K2319/01 , C07K2319/035 , C07K2319/33 , C07K2319/55 , C07K2319/735
摘要： The present invention relates to retroviral constructs that encode novel monoclonal antibodies, novel fusion proteins, and chimeric monoclonal antibodies and to methods of using and producing the same. In particular, the present invention relates to methods of producing a fusion protein comprising a microorganism targeting molecule (e.g., immunoglobulin or innate immune system receptor molecule) and a biocide (e.g., bactericidal enzymes) in transgenic animals (e.g., bovines) and in cell cultures. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in health care (e.g., human and veterinary), agriculture (e.g., animal and plant production), and food processing (e.g., beef carcass processing). The present invention also relates to methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in various diagnostic applications in number of diverse fields such as agriculture, medicine, and national defense.
摘要翻译：本发明涉及编码新单克隆抗体，新型融合蛋白和嵌合单克隆抗体的逆转录病毒构建体及其使用和生产方法。特别地，本发明涉及在转基因动物（例如牛）和细胞中产生包含微生物靶向分子（例如，免疫球蛋白或先天免疫系统受体分子）和杀生物剂（例如杀菌酶）的融合蛋白的方法。文化。本发明还涉及在医疗保健（例如人类和兽医），农业（例如动物和植物生产）和食品加工（例如，动物和植物生产）中使用包含微生物靶向分子和杀生物剂的融合蛋白的治疗和预防方法，牛肉屠体处理）。本发明还涉及在农业，医药，国防等不同领域的多种诊断应用中使用包含微生物靶向分子和杀生物剂的融合蛋白的方法。

17. 发明申请

US20100009869A1 ANTIBODY LIBRARIES 有权
标题翻译：抗体图书馆
公开(公告)号：US20100009869A1
公开(公告)日：2010-01-14
申请号：US12493851
申请日：2009-06-29
申请人： Robert D. Bremel , Kurt Eakle , Michael Imboden
发明人： Robert D. Bremel , Kurt Eakle , Michael Imboden
IPC分类号： C40B40/02
CPC分类号： C07K16/00 , C07K2317/21 , C12N2799/027
摘要： The present invention relates to the production of antibody libraries. In particular, the present invention relates to the use of integrating retroviral vectors to generate libraries comprising a plurality of combinations of antibody light chains and heavy chains. The present invention thus provides improved methods of generating and screening antibody libraries comprising large numbers of unique antibodies.
摘要翻译：本发明涉及抗体文库的生产。特别地，本发明涉及整合逆转录病毒载体以产生包含抗体轻链和重链的多种组合的文库的用途。因此，本发明提供了产生和筛选包含大量独特抗体的抗体文库的改进方法。

18. 发明申请

US20090098121A1 Immunoglobulin Directed Biocides 审中-公开
标题翻译：免疫球蛋白导向杀生物剂
公开(公告)号：US20090098121A1
公开(公告)日：2009-04-16
申请号：US11875339
申请日：2007-10-19
申请人： Michael Imboden , Jane Homan , Robert D. Bremel
发明人： Michael Imboden , Jane Homan , Robert D. Bremel
IPC分类号： A61K39/395 , C12N15/63 , C07K16/00 , C12N1/21 , C12N1/15 , C12N7/00 , C12N1/00 , C12N5/10 , A23J1/00
CPC分类号： A01N63/04 , A01N63/00 , A01N63/02 , A01N65/00 , C07K16/00 , C07K16/20 , C07K2317/52 , C07K2319/01 , C07K2319/035 , C07K2319/33 , C07K2319/55 , C07K2319/735
摘要： The present invention relates to retroviral constructs that encode novel monoclonal antibodies, novel fusion proteins, and chimeric monoclonal antibodies and to methods of using and producing the same. In particular, the present invention relates to methods of producing a fusion protein comprising a microorganism targeting molecule (e.g., immunoglobulin or innate immune system receptor molecule) and a biocide (e.g., bactericidal enzymes) in transgenic animals (e.g., bovines) and in cell cultures. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in health care (e.g., human and veterinary), agriculture (e.g., animal and plant production), and food processing (e.g., beef carcass processing). The present invention also relates to methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in various diagnostic applications in number of diverse fields such as agriculture, medicine, and national defense.
摘要翻译：本发明涉及编码新单克隆抗体，新型融合蛋白和嵌合单克隆抗体的逆转录病毒构建体及其使用和生产方法。特别地，本发明涉及在转基因动物（例如牛）和细胞中产生包含微生物靶向分子（例如，免疫球蛋白或先天免疫系统受体分子）和杀生物剂（例如杀菌酶）的融合蛋白的方法。文化。本发明还涉及在医疗保健（例如人类和兽医），农业（例如动物和植物生产）和食品加工（例如，动物和植物生产）中使用包含微生物靶向分子和杀生物剂的融合蛋白的治疗和预防方法，牛肉屠体处理）。本发明还涉及在农业，医药，国防等不同领域的多种诊断应用中使用包含微生物靶向分子和杀生物剂的融合蛋白的方法。

19. 发明授权

US07384738B2 Retrovirus-based genomic screening 有权
标题翻译：基于逆转录病毒的基因组筛选
公开(公告)号：US07384738B2
公开(公告)日：2008-06-10
申请号：US10397569
申请日：2003-03-26
申请人： Robert D. Bremel , Gregory T. Bleck , Michael Imboden , Kurt Eakle
发明人： Robert D. Bremel , Gregory T. Bleck , Michael Imboden , Kurt Eakle
IPC分类号： C12Q1/68
CPC分类号： C12N15/1082 , C12N7/00 , C12N15/86 , C12N2740/13043 , C12N2740/13052 , C12N2830/42 , C12N2830/48 , C12N2840/203
摘要： The present invention relates to the expression and screening of genomic DNA sequences encoding uncharacterized genes and proteins. The present invention provides systems utilizing unique features of retroviral replication to analyze uncharacterized genes derived from genomic DNA samples. In preferred embodiments, a segment of genomic DNA is inserted between 5′ and 3′ viral long terminal repeats (LTRs) in a vector (e.g., a plasmid, cosmid, or artificial chromosome vector). The resulting vector (or library of vectors containing a plurality of independent genomic sequences) is then introduced into a retroviral packaging cell. The resulting provirus or proteins expression from the provirus are then analyzed.
摘要翻译：本发明涉及编码非特异性基因和蛋白质的基因组DNA序列的表达和筛选。本发明提供利用逆转录病毒复制的独特特征来分析源自基因组DNA样品的未表征基因的系统。在优选的实施方案中，将一段基因组DNA插入载体（例如质粒，粘粒或人造染色体载体）中的5'和3'病毒长末端重复序列（LTR）之间。然后将所得载体（或含有多个独立基因组序列的载体文库）导入逆转录病毒包装细胞。然后分析来自前病毒的所得原病毒或蛋白质的表达。

20. 发明授权

US06852510B2 Host cells containing multiple integrating vectors 有权
标题翻译：含有多个整合载体的宿主细胞
公开(公告)号：US06852510B2
公开(公告)日：2005-02-08
申请号：US09897511
申请日：2001-06-29
申请人： Robert D. Bremel , Linda U. Miller , Gregory T. Bleck
发明人： Robert D. Bremel , Linda U. Miller , Gregory T. Bleck
IPC分类号： C07K14/02 , C07K16/12 , C07K16/30 , C12N7/04 , C12N15/82 , C12N15/867 , C12P21/02 , C12N15/09 , C12N5/10 , C12N15/63
CPC分类号： C12N15/86 , C07K14/005 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N2510/02 , C12N2730/10122 , C12N2740/13023 , C12N2740/13043 , C12N2740/13051 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12P21/02
摘要： The present invention relates to the production of proteins in host cells, and, more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.
摘要翻译：本发明涉及在宿主细胞中产生蛋白质，更具体地涉及含有多个整合载体的集成拷贝的宿主细胞。用于本发明的合适的整合载体包括逆转录病毒载体，慢病毒载体，转座子载体和腺相关病毒载体。提供了其中通过以高倍数感染使用整合载体制备宿主细胞的方法。宿主细胞可用于生产药物蛋白质，用于筛选测定的蛋白质变体，以及直接用于高通量筛选。

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