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1. 发明授权

US07384738B2 Retrovirus-based genomic screening 有权
标题翻译：基于逆转录病毒的基因组筛选
公开(公告)号：US07384738B2
公开(公告)日：2008-06-10
申请号：US10397569
申请日：2003-03-26
申请人： Robert D. Bremel , Gregory T. Bleck , Michael Imboden , Kurt Eakle
发明人： Robert D. Bremel , Gregory T. Bleck , Michael Imboden , Kurt Eakle
IPC分类号： C12Q1/68
CPC分类号： C12N15/1082 , C12N7/00 , C12N15/86 , C12N2740/13043 , C12N2740/13052 , C12N2830/42 , C12N2830/48 , C12N2840/203
摘要： The present invention relates to the expression and screening of genomic DNA sequences encoding uncharacterized genes and proteins. The present invention provides systems utilizing unique features of retroviral replication to analyze uncharacterized genes derived from genomic DNA samples. In preferred embodiments, a segment of genomic DNA is inserted between 5′ and 3′ viral long terminal repeats (LTRs) in a vector (e.g., a plasmid, cosmid, or artificial chromosome vector). The resulting vector (or library of vectors containing a plurality of independent genomic sequences) is then introduced into a retroviral packaging cell. The resulting provirus or proteins expression from the provirus are then analyzed.
摘要翻译：本发明涉及编码非特异性基因和蛋白质的基因组DNA序列的表达和筛选。本发明提供利用逆转录病毒复制的独特特征来分析源自基因组DNA样品的未表征基因的系统。在优选的实施方案中，将一段基因组DNA插入载体（例如质粒，粘粒或人造染色体载体）中的5'和3'病毒长末端重复序列（LTR）之间。然后将所得载体（或含有多个独立基因组序列的载体文库）导入逆转录病毒包装细胞。然后分析来自前病毒的所得原病毒或蛋白质的表达。

2. 发明授权

US08222188B2 Antibody libraries 有权
标题翻译：抗体库
公开(公告)号：US08222188B2
公开(公告)日：2012-07-17
申请号：US12493851
申请日：2009-06-29
申请人： Robert D. Bremel , Kurt Eakle , Michael Imboden
发明人： Robert D. Bremel , Kurt Eakle , Michael Imboden
IPC分类号： C40B40/02 , C12Q1/70
CPC分类号： C07K16/00 , C07K2317/21 , C12N2799/027
摘要： The present invention relates to the production of antibody libraries. In particular, the present invention relates to the use of integrating retroviral vectors to generate libraries comprising a plurality of combinations of antibody light chains and heavy chains. The present invention thus provides improved methods of generating and screening antibody libraries comprising large numbers of unique antibodies.
摘要翻译：本发明涉及抗体文库的生产。特别地，本发明涉及整合逆转录病毒载体以产生包含抗体轻链和重链的多种组合的文库的用途。因此，本发明提供了产生和筛选包含大量独特抗体的抗体文库的改进方法。

3. 发明申请

US20100009869A1 ANTIBODY LIBRARIES 有权
标题翻译：抗体图书馆
公开(公告)号：US20100009869A1
公开(公告)日：2010-01-14
申请号：US12493851
申请日：2009-06-29
申请人： Robert D. Bremel , Kurt Eakle , Michael Imboden
发明人： Robert D. Bremel , Kurt Eakle , Michael Imboden
IPC分类号： C40B40/02
CPC分类号： C07K16/00 , C07K2317/21 , C12N2799/027
摘要： The present invention relates to the production of antibody libraries. In particular, the present invention relates to the use of integrating retroviral vectors to generate libraries comprising a plurality of combinations of antibody light chains and heavy chains. The present invention thus provides improved methods of generating and screening antibody libraries comprising large numbers of unique antibodies.
摘要翻译：本发明涉及抗体文库的生产。特别地，本发明涉及整合逆转录病毒载体以产生包含抗体轻链和重链的多种组合的文库的用途。因此，本发明提供了产生和筛选包含大量独特抗体的抗体文库的改进方法。

4. 发明授权

US5530177A Transgenic mice producing bovine .alpha.-lactalbumin in their milk 失效
标题翻译：在其牛奶中产生牛α-乳白蛋白的转基因小鼠
公开(公告)号：US5530177A
公开(公告)日：1996-06-25
申请号：US71601
申请日：1993-06-04
申请人： Gregory T. Bleck , Robert D. Bremel
发明人： Gregory T. Bleck , Robert D. Bremel
IPC分类号： C12N15/09 , A01K67/027 , C07K14/76 , C12N15/85 , C12P21/02 , C12N5/00 , C07H21/04 , C12N15/00 , C12N15/63
CPC分类号： C12N15/8509 , A01K67/0275 , C07K14/76 , A01K2217/05 , A01K2227/10 , A01K2227/105 , A01K2267/01
摘要： The present invention relates to the use of a mammary specific .alpha.-lactalbumin regulatory sequence to assist in the production of recombinant proteins in mammals' milk. The invention also relates to the genetically engineered mammal that produces the desired recombinant product in its milk and to the products produced by the genetically engineered mammal, including the altered composition of milk and the semen which includes the desired .alpha.-lactalbumin protein DNA sequence.
摘要翻译：本发明涉及乳腺特异性α-乳白蛋白调节序列在哺乳动物乳中生产重组蛋白质的用途。本发明还涉及在其牛奶中产生所需重组产物和由遗传工程哺乳动物产生的产品的基因工程哺乳动物，包括改变的乳组合物和包含所需α-乳白蛋白DNA序列的精液。

5. 发明授权

US07332333B2 Host cells containing multiple integrating vectors 有权
标题翻译：含有多个整合载体的宿主细胞
公开(公告)号：US07332333B2
公开(公告)日：2008-02-19
申请号：US11018895
申请日：2004-12-21
申请人： Robert D. Bremel , Linda U. Miller , Gregory T. Bleck
发明人： Robert D. Bremel , Linda U. Miller , Gregory T. Bleck
IPC分类号： C12N15/867 , C12N5/10
CPC分类号： C12N15/86 , C07K14/005 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N2510/02 , C12N2730/10122 , C12N2740/13023 , C12N2740/13043 , C12N2740/13051 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12P21/02
摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.
摘要翻译：本发明涉及宿主细胞中蛋白质的制备，更具体地涉及含有整合载体的多个整合拷贝的宿主细胞。用于本发明的合适的整合载体包括逆转录病毒载体，慢病毒载体，转座子载体和腺相关病毒载体。提供了其中通过以高倍数感染使用整合载体制备宿主细胞的方法。宿主细胞可用于生产药物蛋白质，用于筛选测定的蛋白质变体，以及直接用于高通量筛选。

6. 发明授权

US06852510B2 Host cells containing multiple integrating vectors 有权
标题翻译：含有多个整合载体的宿主细胞
公开(公告)号：US06852510B2
公开(公告)日：2005-02-08
申请号：US09897511
申请日：2001-06-29
申请人： Robert D. Bremel , Linda U. Miller , Gregory T. Bleck
发明人： Robert D. Bremel , Linda U. Miller , Gregory T. Bleck
IPC分类号： C07K14/02 , C07K16/12 , C07K16/30 , C12N7/04 , C12N15/82 , C12N15/867 , C12P21/02 , C12N15/09 , C12N5/10 , C12N15/63
CPC分类号： C12N15/86 , C07K14/005 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N2510/02 , C12N2730/10122 , C12N2740/13023 , C12N2740/13043 , C12N2740/13051 , C12N2830/00 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12P21/02
摘要： The present invention relates to the production of proteins in host cells, and, more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.
摘要翻译：本发明涉及在宿主细胞中产生蛋白质，更具体地涉及含有多个整合载体的集成拷贝的宿主细胞。用于本发明的合适的整合载体包括逆转录病毒载体，慢病毒载体，转座子载体和腺相关病毒载体。提供了其中通过以高倍数感染使用整合载体制备宿主细胞的方法。宿主细胞可用于生产药物蛋白质，用于筛选测定的蛋白质变体，以及直接用于高通量筛选。

7. 发明授权

US5850000A Transgenic non-human mammals comprising a bovine 5' flanking regulatory sequence 失效
标题翻译：包含牛5'侧翼调节序列的转基因非人哺乳动物
公开(公告)号：US5850000A
公开(公告)日：1998-12-15
申请号：US621100
申请日：1996-03-22
申请人： Gregory T. Bleck , Robert D. Bremel
发明人： Gregory T. Bleck , Robert D. Bremel
IPC分类号： C12N15/09 , A01K67/027 , C07K14/76 , C12N15/85 , C12P21/02 , C12N5/00 , C07H21/04 , C12N15/00 , C12P21/06
CPC分类号： C12N15/8509 , A01K67/0275 , C07K14/76 , A01K2217/05 , A01K2227/10 , A01K2227/105 , A01K2267/01
摘要： The present invention relates to the use of a mammary specific .alpha.-lactalbumin protein to assist in the production of recombinant proteins in mammals' milk. The invention also relates to the genetically engineered mammal that produces the desired recombinant product in its milk and to the products produced by the genetically engineered mammal, including the altered composition of milk and the semen which includes the desired .alpha.-lactalbumin protein DNA sequence.
摘要翻译：本发明涉及乳腺特异性α-乳白蛋白蛋白在哺乳动物乳中生产重组蛋白质的用途。本发明还涉及在其牛奶中产生所需重组产物和由遗传工程哺乳动物产生的产品的基因工程哺乳动物，包括改变的乳组合物和包含所需α-乳白蛋白DNA序列的精液。

8. 发明授权

US07300790B2 Transgenic animals expressing transdominant negative retroviral nucleic acids and proteins 失效
标题翻译：转基因动物表达显性阴性逆转录病毒核酸和蛋白质
公开(公告)号：US07300790B2
公开(公告)日：2007-11-27
申请号：US10764201
申请日：2004-01-23
申请人： Kurt Eakle , Thomas Hope , Eun-A Choi , Jane Homan , Robert D. Bremel
发明人： Kurt Eakle , Thomas Hope , Eun-A Choi , Jane Homan , Robert D. Bremel
IPC分类号： C12P1/00 , C12P37/04
CPC分类号： C07K14/005 , A01K2217/05 , A01K2227/101 , A01K2267/02 , A61K48/00 , C12N2740/14022 , C12N2740/14043 , C12N2830/00
摘要： The present invention relates to non-vaccinal and non-pharmacologic compositions and methods for controlling complex retroviral infections. In particular, the present invention provides transgenic animals expressing a transdominant negative Rex gene product that inhibits retroviral replication.
摘要翻译：本发明涉及用于控制复合逆转录病毒感染的非疫苗和非药物组合物和方法。特别地，本发明提供表达抑制逆转录病毒复制的显性阴性Rex基因产物的转基因动物。

9. 发明申请

US20080269122A1 Antimicrobial Peptide Biocides 审中-公开
标题翻译：抗微生物肽杀菌剂
公开(公告)号：US20080269122A1
公开(公告)日：2008-10-30
申请号：US11875349
申请日：2007-10-19
申请人： Michael Imboden , Jane Homan , Robert D. Bremel
发明人： Michael Imboden , Jane Homan , Robert D. Bremel
IPC分类号： A61K38/00 , C12N15/00 , C12N5/06 , A61P31/00 , A23L3/3463
CPC分类号： A01N63/04 , A01N63/00 , A01N63/02 , A01N65/00 , C07K16/00 , C07K16/20 , C07K2317/52 , C07K2319/01 , C07K2319/035 , C07K2319/33 , C07K2319/55 , C07K2319/735
摘要： The present invention relates to retroviral constructs that encode novel monoclonal antibodies, novel fusion proteins, and chimeric monoclonal antibodies and to methods of using and producing the same. In particular, the present invention relates to methods of producing a fusion protein comprising a microorganism targeting molecule (e.g., immunoglobulin or innate immune system receptor molecule) and a biocide (e.g., bactericidal enzymes) in transgenic animals (e.g., bovines) and in cell cultures. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in health care (e.g., human and veterinary), agriculture (e.g., animal and plant production), and food processing (e.g., beef carcass processing). The present invention also relates to methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in various diagnostic applications in number of diverse fields such as agriculture, medicine, and national defense.
摘要翻译：本发明涉及编码新单克隆抗体，新型融合蛋白和嵌合单克隆抗体的逆转录病毒构建体及其使用和生产方法。特别地，本发明涉及在转基因动物（例如牛）和细胞中产生包含微生物靶向分子（例如，免疫球蛋白或先天免疫系统受体分子）和杀生物剂（例如杀菌酶）的融合蛋白的方法。文化。本发明还涉及在医疗保健（例如人类和兽医），农业（例如动物和植物生产）和食品加工（例如，动物和植物生产）中使用包含微生物靶向分子和杀生物剂的融合蛋白的治疗和预防方法，牛肉屠体处理）。本发明还涉及在农业，医药，国防等不同领域的多种诊断应用中使用包含微生物靶向分子和杀生物剂的融合蛋白的方法。

10. 发明申请

US20080267943A1 Innate Immune Receptor Directed Biocides 审中-公开
标题翻译：先天免疫受体导向杀生物剂
公开(公告)号：US20080267943A1
公开(公告)日：2008-10-30
申请号：US11875343
申请日：2007-10-19
申请人： Michael Imboden , Jane Homan , Robert D. Bremel
发明人： Michael Imboden , Jane Homan , Robert D. Bremel
IPC分类号： A61K38/43 , A61K38/00 , C12N5/06 , C12N15/00 , A01K67/027 , A61P31/00 , A23L3/3463
CPC分类号： A01N63/04 , A01N63/00 , A01N63/02 , A01N65/00 , C07K16/00 , C07K16/20 , C07K2317/52 , C07K2319/01 , C07K2319/035 , C07K2319/33 , C07K2319/55 , C07K2319/735
摘要： The present invention relates to retroviral constructs that encode novel monoclonal antibodies, novel fusion proteins, and chimeric monoclonal antibodies and to methods of using and producing the same. In particular, the present invention relates to methods of producing a fusion protein comprising a microorganism targeting molecule (e.g., immunoglobulin or innate immune system receptor molecule) and a biocide (e.g., bactericidal enzymes) in transgenic animals (e.g., bovines) and in cell cultures. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in health care (e.g., human and veterinary), agriculture (e.g., animal and plant production), and food processing (e.g., beef carcass processing). The present invention also relates to methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in various diagnostic applications in number of diverse fields such as agriculture, medicine, and national defense.
摘要翻译：本发明涉及编码新单克隆抗体，新型融合蛋白和嵌合单克隆抗体的逆转录病毒构建体及其使用和生产方法。特别地，本发明涉及在转基因动物（例如牛）和细胞中产生包含微生物靶向分子（例如，免疫球蛋白或先天免疫系统受体分子）和杀生物剂（例如杀菌酶）的融合蛋白的方法。文化。本发明还涉及在医疗保健（例如人类和兽医），农业（例如动物和植物生产）和食品加工（例如，动物和植物生产）中使用包含微生物靶向分子和杀生物剂的融合蛋白的治疗和预防方法，牛肉屠体处理）。本发明还涉及在农业，医药，国防等不同领域的多种诊断应用中使用包含微生物靶向分子和杀生物剂的融合蛋白的方法。

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