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11. 发明申请

US20090117549A1 Aptamer-based methods for identifying cellular biomarkers 审中-公开
标题翻译：用于鉴定细胞生物标志物的基于探针的方法
公开(公告)号：US20090117549A1
公开(公告)日：2009-05-07
申请号：US11880013
申请日：2007-07-18
申请人： Weihong Tan , Dihua Shangguan
发明人： Weihong Tan , Dihua Shangguan
IPC分类号： C12Q1/68 , G01N33/566 , B01D59/44
CPC分类号： C12N15/115 , C12N15/111 , C12N2310/16 , C12N2320/11
摘要： In this invention, a biomarker discovery method has been developed using specific biotin-labeled oligonucleotide ligands and magnetic streptavidin beads. In one embodiment, the oligonucleotide ligands are firstly generated by whole-cell based SELEX technique. Such ligands can recognize target cells with high affinity and specificity and can distinguish cells that are closely related to target cells even in patient samples. The targets of these oligonucleotide ligands are significant biomarkers for certain cells. These important biomarkers can be captured by forming complexes with biotin-labeled oligonucleotide ligands and collecting the complexes using magnetic streptavidin beads, whereupon the captured biomarkers are analyzed to identify the biomarkers. Analysis of biomarkers include HPLC-Mass Spectroscopy analysis, polyacrylamide gel electrophoresis, flow cytometry, and the like. The identified biomarkers can be used for pathological diagnosis and therapeutic applications. Using the disclosed methods, highly specific biomarkers of any kinds of cells, in particular cancer cells, can easily be identified without prior knowledge of the existence of such biomarkers.
摘要翻译：在本发明中，已经开发了使用特定生物素标记的寡核苷酸配体和磁性链亲和素珠子的生物标志物发现方法。在一个实施方案中，寡核苷酸配体首先通过基于全细胞的SELEX技术产生。这样的配体可以高亲和力和特异性识别靶细胞，并且即使在患者样品中也能区分与靶细胞密切相关的细胞。这些寡核苷酸配体的靶标是某些细胞的重要生物标志物。可以通过与生物素标记的寡核苷酸配体形成复合物并使用磁性链亲和素珠来收集复合物来捕获这些重要的生物标志物，然后分析捕获的生物标志物以鉴定生物标志物。生物标志物的分析包括HPLC-质谱分析，聚丙烯酰胺凝胶电泳，流式细胞术等。鉴定的生物标志物可用于病理诊断和治疗应用。使用所公开的方法，可以容易地鉴定任何种类的细胞，特别是癌细胞的高度特异性生物标志物，而无需存在这样的生物标志物。

12. 发明授权

US07524630B2 Functionalized nanoparticles and methods of use 失效
标题翻译：官能化纳米粒子和使用方法
公开(公告)号：US07524630B2
公开(公告)日：2009-04-28
申请号：US10421491
申请日：2003-04-22
申请人： Weihong Tan , Jin Shouguang , Xiaojun Zhao , Rovelyn Tapec Dytioco , Timothy James Drake , Lisa Renee Hilliard
发明人： Weihong Tan , Jin Shouguang , Xiaojun Zhao , Rovelyn Tapec Dytioco , Timothy James Drake , Lisa Renee Hilliard
IPC分类号： G01N33/552 , G01N33/553
CPC分类号： G01N33/54346 , B82Y15/00 , C12Q1/6816 , G01N33/54393 , G01N33/552 , G01N33/588 , C12Q2563/155
摘要： Silica-coated nanoparticles functionalized with biologically active molecules such as antibodies and nucleotides are used to label cells, to detect and isolate nucleic acid molecules having specific nucleotide sequences, and to separate a mixture of different nucleic acid molecules.
摘要翻译：用生物活性分子（如抗体和核苷酸）功能化的二氧化硅包被的纳米粒子用于标记细胞，以检测和分离具有特定核苷酸序列的核酸分子，并分离不同核酸分子的混合物。

13. 发明申请

US20080311590A1 Portable Materials and Methods for Ultrasensitive Detection of Pathogen and Bioparticles 审中-公开
标题翻译：便携式材料和方法用于超敏感检测病原体和生物颗粒
公开(公告)号：US20080311590A1
公开(公告)日：2008-12-18
申请号：US11994962
申请日：2006-10-10
申请人： Weihong Tan , Shelly John Mechery
发明人： Weihong Tan , Shelly John Mechery
IPC分类号： G01N33/53 , C12M1/34 , B01J19/00 , G01N21/00 , G01N21/76
CPC分类号： G01N21/6428 , G01N15/1459 , G01N2015/1486
摘要： The present invention provides systems for ultrasensitive detection of pathogens and bioparticles. One embodiment of the system comprises an optical detection scheme that allows for the detection of the fluorescence signal of bacteria or other bioparticles in less than about 20 minutes. A microflow channel allows for an assay probing volume of as little as a few picoliters. In one embodiment, the system uses RuBpy dye-doped silica nanoparticles bioconjugated with specific monoclonal antibodies of the target bioparticles. The system allows for the rapid and highly sensitive and specific detection of bacteria or other bioparticles without the need for amplification or enrichment of the sample.
摘要翻译：本发明提供了用于病原体和生物颗粒的超敏感检测的系统。该系统的一个实施例包括允许在少于约20分钟内检测细菌或其他生物颗粒的荧光信号的光学检测方案。微流通道允许少至几微摩尔的测定探测体积。在一个实施方案中，该系统使用与靶生物颗粒的特异性单克隆抗体生物缀合的RuBpy染料掺杂二氧化硅纳米颗粒。该系统允许对细菌或其他生物颗粒的快速和高灵敏度和特异性检测，而不需要扩增或富集样品。

14. 发明申请

US20060228554A1 Method of making nanoparticles 失效
标题翻译：纳米颗粒的制备方法
公开(公告)号：US20060228554A1
公开(公告)日：2006-10-12
申请号：US11188459
申请日：2005-07-25
申请人： Weihong Tan , Swadeshmukul Santra , Peng Zhang , Rovelyn Tapec , Jon Dobson
发明人： Weihong Tan , Swadeshmukul Santra , Peng Zhang , Rovelyn Tapec , Jon Dobson
IPC分类号： B32B1/00
CPC分类号： G01N33/54346 , A61K49/183 , A61K49/1875 , B01J13/18 , B82Y5/00 , B82Y25/00 , B82Y30/00 , C09B67/0005 , G01N33/54326 , G01N33/552 , G01N33/569 , G01N33/587 , G01N2446/20 , H01F1/0054 , Y10T428/2982 , Y10T428/2984 , Y10T428/2989 , Y10T428/2991 , Y10T428/2993 , Y10T428/2995
摘要： Disclosed are silica-coated nanoparticles and a process for producing silica-coated nanoparticles. Silica-coated nanoparticles are prepared by precipitating nano-sized cores from reagents dissolved in the aqueous compartment of a water-in-oil microemulsion. A reactive silicate is added to coat the cores with silica. Also disclosed are methods for functionalizing silica-coated nanoparticles for use in a variety of applications.
摘要翻译：公开了二氧化硅涂覆的纳米颗粒和用于生产二氧化硅涂覆的纳米颗粒的方法。通过从溶解在油包水微乳液的水性隔室中的试剂中沉淀纳米尺寸的核来制备二氧化硅涂覆的纳米颗粒。加入反应性硅酸盐以用二氧化硅涂覆芯。还公开了用于在各种应用中使用的二氧化硅涂覆的纳米颗粒的官能化方法。

15. 发明申请

US20120087949A1 OLIGONUCLEOTIDE MICELLES 审中-公开
标题翻译：寡核苷酸
公开(公告)号：US20120087949A1
公开(公告)日：2012-04-12
申请号：US13141027
申请日：2009-12-18
申请人： Weihong Tan , Liu Haipeng , Yanrong Wu
发明人： Weihong Tan , Liu Haipeng , Yanrong Wu
IPC分类号： A61K31/7088 , C12Q1/02 , C12N5/00 , A61K9/00
CPC分类号： A61K31/7088 , A61K9/0019 , A61K9/1075 , C12N15/111 , C12N2310/3515 , C12N2320/32
摘要： The present invention provides homogenous populations of micelles, methods for preparing these populations, methods for treating or preventing a disease or disorder using the population of micelles of the invention.
摘要翻译：本发明提供了均匀的胶束群，制备这些种群的方法，使用本发明的胶束群治疗或预防疾病或病症的方法。

16. 发明申请

US20090156416A1 Hybrid Molecular Probe 审中-公开
标题翻译：杂交分子探针
公开(公告)号：US20090156416A1
公开(公告)日：2009-06-18
申请号：US11994965
申请日：2006-10-11
申请人： Weihong Tan , Chaoyong Yang
发明人： Weihong Tan , Chaoyong Yang
IPC分类号： C40B30/04 , C07H21/00 , C07K14/00 , G01N33/48
CPC分类号： C12Q1/6876 , Y10T436/143333
摘要： A system and method for analyzing a substance, in particular RNA in vivo, comprising a hybrid molecular probe, said probe comprising two single-stranded nucleic acid sequences tethered together with a polyethylene glycol polymer and fluorophores attached to either end of the sequences. When a probe of the invention hybridizes to a target substance (such as a target RKA sequence), fluorescence resonance energy transfer occurs between the two fluorophores to generate a visible signal.
摘要翻译：用于分析物质，特别是体内RNA的系统和方法，其包含杂交分子探针，所述探针包含与聚乙二醇聚合物连接的两条单链核酸序列和连接于序列任一端的荧光团。当本发明的探针与目标物质（例如目标RKA序列）杂交时，在两个荧光团之间发生荧光共振能量转移以产生可见信号。

17. 发明授权

US07263648B2 Apparatus and method for accommodating loss of signal 有权
标题翻译：用于容纳信号损失的装置和方法
公开(公告)号：US07263648B2
公开(公告)日：2007-08-28
申请号：US10350930
申请日：2003-01-24
申请人： Dennis Blackburn , Kenneth Osburn , Robert Buggy , Weihong Tan
发明人： Dennis Blackburn , Kenneth Osburn , Robert Buggy , Weihong Tan
IPC分类号： G11C29/00 , G06F13/00
CPC分类号： H04H60/06 , H04H20/38 , H04H60/12 , H04H60/27 , H04N7/162 , H04N21/4331 , H04N21/434 , H04N21/44016 , H04N21/4425 , H04N21/812
摘要： A memory control system for a network that broadcasts to multiple terminals content data including music, video and the like, and also including commercial advertisements. A memory in the terminal unit stores content data that is played upon a loss of signal from the broadcast network. The memory also stores commercial advertisements for playback from the terminal memory according to schedule control instructions, which are also stored in a memory at the terminal unit. Writing of content to a memory and reading content from the memory may be executed concurrently by interleaving. A microprocessor arbitrates use of input, output and memory buffers such that reading content from memory provides content output maintaining uninterrupted real time play even when new content data is being stored concurrently. Content data memory addresses are stored in sector tables that are further arranged in File Allocation Tables in a second memory.
摘要翻译：一种用于网络的存储器控制系统，其向多个终端广播包括音乐，视频等的内容数据，并且还包括商业广告。终端单元中的存储器存储当从广播网络丢失信号时播放的内容数据。存储器还存储根据计划控制指令从终端存储器回放的商业广告，其也存储在终端单元的存储器中。将内容写入存储器并从存储器读取内容可以通过交错同时执行。微处理器对使用输入，输出和存储器缓冲器进行仲裁，使得即使当新的内容数据被同时存储时，从存储器读取内容也提供内容输出维持不间断的实时播放。内容数据存储器地址被存储在进一步被布置在第二存储器中的文件分配表中的扇区表中。

18. 发明授权

US06548264B1 Coated nanoparticles 失效
标题翻译：涂层纳米粒子
公开(公告)号：US06548264B1
公开(公告)日：2003-04-15
申请号：US09572469
申请日：2000-05-17
申请人： Weihong Tan , Swadeshmukul Santra , Peng Zhang , Rovelyn Tapec , Jon Dobson
发明人： Weihong Tan , Swadeshmukul Santra , Peng Zhang , Rovelyn Tapec , Jon Dobson
IPC分类号： G01N33553
CPC分类号： G01N33/54346 , A61K49/183 , A61K49/1875 , B01J13/18 , B82Y5/00 , B82Y25/00 , B82Y30/00 , C09B67/0005 , G01N33/54326 , G01N33/552 , G01N33/569 , G01N33/587 , G01N2446/20 , H01F1/0054 , Y10T428/2982 , Y10T428/2984 , Y10T428/2989 , Y10T428/2991 , Y10T428/2993 , Y10T428/2995
摘要： Disclosed are silica-coated nanoparticles and a process for producing silica-coated nanoparticles. Silica-coated nanoparticles are prepared by precipitating nano-sized cores from reagents dissolved in the aqueous compartment of a water-in-oil microemulsion. A reactive silicate is added to coat the cores with silica. Also disclosed are methods for functionalizing silica-coated nanoparticles for use in a variety of applications.
摘要翻译：公开了二氧化硅涂覆的纳米颗粒和用于生产二氧化硅涂覆的纳米颗粒的方法。通过从溶解在油包水微乳液的水性隔室中的试剂中沉淀纳米尺寸的核来制备二氧化硅涂覆的纳米颗粒。加入反应性硅酸盐以用二氧化硅涂覆芯。还公开了用于在各种应用中使用的二氧化硅涂覆的纳米颗粒的官能化方法。

19. 发明授权

US5627922A Micro optical fiber light source and sensor and method of fabrication thereof 失效
标题翻译：微光纤光源和传感器及其制造方法
公开(公告)号：US5627922A
公开(公告)日：1997-05-06
申请号：US397331
申请日：1995-03-02
申请人： Raoul Kopelman , Weihong Tan , Zhong-You Shi
发明人： Raoul Kopelman , Weihong Tan , Zhong-You Shi
IPC分类号： G01Q60/18 , G02B6/24 , G02B6/255 , G02B6/36 , G02B6/00 , G01J1/04 , G02B21/00
CPC分类号： G02B6/241 , B82Y20/00 , B82Y35/00 , G01Q60/22 , G02B6/2552 , G02B6/3624
摘要： This invention relates generally to the development of and a method of fabricating a fiber optic micro-light source and sensor (50). An optical fiber micro-light source (50) is presented whose aperture is extremely small yet able to act as an intense light source. Light sources of this type have wide ranging applications, including use as micro-sensors (22) in NSOM. Micro-sensor light sources have excellent detection limits as well as photo stability, reversibility, and millisecond response times. Furthermore, a method for manufacturing a micro optical fiber light source is provided. It involves the photo-chemical attachment of an optically active material onto the end surface of an optical fiber cable which has been pulled to form an end with an extremely narrow aperture. More specifically, photopolymerization has been applied as a means to photo-chemically attach an optically active material (60). This process allows significant control of the size of the micro light source (50). Furthermore, photo-chemically attaching an optically active material (60) enables the implementation of the micro-light source in a variety of sensor applications.
摘要翻译：本发明一般涉及光纤微光源和传感器（50）的制造和制造方法。提出了一种光纤微光源（50），其光圈非常小，但能够充当强光源。这种光源具有广泛的应用，包括在NSOM中用作微型传感器（22）。微传感器光源具有优异的检测限以及光稳定性，可逆性和毫秒响应时间。此外，提供了一种用于制造微型光纤光源的方法。它涉及光学活性材料的光化学附着到光纤电缆的端面上，该光缆被拉动以形成具有非常窄的孔径的端部。更具体地，已经应用光聚合作为光学化学连接光学活性材料（60）的手段。该过程允许显着控制微光源（50）的尺寸。此外，光学化学连接光学活性材料（60）使得能够在各种传感器应用中实现微光源。

20. 发明授权

US08652851B2 Multi-acceptor molecular probes and applications thereof 失效
标题翻译：多受体分子探针及其应用
公开(公告)号：US08652851B2
公开(公告)日：2014-02-18
申请号：US11570491
申请日：2005-06-17
申请人： Weihong Tan , Chaoyong Yang
发明人： Weihong Tan , Chaoyong Yang
IPC分类号： G01N21/76
CPC分类号： G01N33/542 , G01N33/5308 , Y10T436/143333
摘要： An oligonucleotide-based molecular probe includes at least one pin loop, the pin loop including a loop sequence complementary to a target sequence. A first stem sequence is attached to one end of the pin loop, the first stem having at least one fluorescent label attached thereto. A second stem sequence is attached to the other end of the pin loop. The second stem has a plurality of quencher molecules attached thereto.
摘要翻译：基于寡核苷酸的分子探针包括至少一个引脚环，引脚环包括与靶序列互补的环序列。第一茎序列连接到针环的一端，第一茎具有至少一个附着于其上的荧光标记。第二个茎序列连接到针环的另一端。第二茎具有多个与其连接的猝灭剂分子。

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