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11. 发明申请

US20080199959A1 Method For Cell Culture 审中-公开
标题翻译：细胞培养方法
公开(公告)号：US20080199959A1
公开(公告)日：2008-08-21
申请号：US11917169
申请日：2006-06-19
申请人： Mattias Algotsson , Gunnar Glad , Nicolas Thevenin
发明人： Mattias Algotsson , Gunnar Glad , Nicolas Thevenin
IPC分类号： C12N5/06 , C12N1/20 , C12N1/00 , C12M1/00
CPC分类号： C12N5/0075 , G01N33/5005 , G01N33/5432
摘要： The present invention relates to a method for cell culture, more precisely small scale cell culture. In the present invention a screening tool is used which comprises particulate matter or microcarriers, such as beads, attached to a solid support, such as a microtiter plate, for the cultivation of cells on said microcarriers. The microcarriers are preferably cultivation beads, such as CYTODEX™. According to the invention, this small scale format for cell cultivation may be used for any testing involving cells, for example testing of optimal growth conditions for a specific type of cell, such as stem cells. Another use is cell expansion.
摘要翻译：本发明涉及一种细胞培养方法，更准确地说是小规模细胞培养。在本发明中，使用了一种筛选工具，其包括连接到固体支持物例如微量滴定板上的颗粒物质或微载体，例如珠，用于在所述微载体上培养细胞。微载体优选是培养珠，例如CYTODEX TM。根据本发明，用于细胞培养的这种小规模格式可用于涉及细胞的任何测试，例如测试特定类型细胞如干细胞的最佳生长条件。另一个用途是细胞扩增。

12. 发明授权

US07385040B2 Antibody purification 有权
标题翻译：抗体纯化
公开(公告)号：US07385040B2
公开(公告)日：2008-06-10
申请号：US10589718
申请日：2005-02-24
申请人： Bo-Lennart Johansson , Hans J. Johansson , Anders Ljunglöf , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Bo-Lennart Johansson , Hans J. Johansson , Anders Ljunglöf , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： C07K1/18 , C07K1/22
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01D15/3847 , B01J39/07 , B01J39/26 , B01J47/014 , C07K1/165 , B01D15/327
摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
摘要翻译：本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统。在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。

13. 发明授权

US07320755B2 Method of preparing ligands for hydrophobic interaction chromatography 失效
标题翻译：制备疏水相互作用色谱配体的方法
公开(公告)号：US07320755B2
公开(公告)日：2008-01-22
申请号：US10547570
申请日：2004-03-05
申请人： Bo-Lennart Johansson , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Bo-Lennart Johansson , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/325 , B01D15/327 , B01J20/286
摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.
摘要翻译：本发明是制备用于疏水相互作用色谱（HIC）的多峰配体的方法，其包括提供包含硫醇，胺和羰基的环状支架; 用试剂衍生氮以引入主要相互作用; 和所得衍生物的氨解，由此在羰基旁边引入二次相互作用; 其中所述主要相互作用和所述第二相互作用中的至少一种包含疏水基团，并且其中所述相互作用中的非相互作用包括带电基团，即离子交换配体。本发明还包括一种分离介质，其包含固定在基础基质上的这种多峰配体。

14. 发明申请

US20070167613A1 Process for the purification of antibodies 有权
标题翻译：抗体纯化方法
公开(公告)号：US20070167613A1
公开(公告)日：2007-07-19
申请号：US10589717
申请日：2005-02-25
申请人： Bo-Lennart Johansson , Hans Johansson , Anna Gronberg , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Bo-Lennart Johansson , Hans Johansson , Anna Gronberg , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： C07K16/18
CPC分类号： B01J20/3253 , B01D15/3847 , B01J20/3242 , B01J20/3246 , B01J20/3255 , B01J20/3285
摘要： The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
摘要翻译：本发明涉及一种从液体中的一种或多种杂质纯化抗体的方法，该方法包括使所述液体与包含多元配体的载体的第一层析树脂接触以将抗体吸附到所述树脂，其中每个多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统; 添加洗脱液以从树脂中释放抗体; 并将如此获得的洗脱液与第二层析树脂接触。在一个实施方案中，芳族或杂芳族实体的成环原子选自C，S和O，阳离子交换基团是弱阳离子交换剂。

15. 发明授权

US07294743B2 Method for synthesis of acrylamide derivatives 有权
标题翻译：合成丙烯酰胺衍生物的方法
公开(公告)号：US07294743B2
公开(公告)日：2007-11-13
申请号：US10572294
申请日：2004-10-18
申请人： Mattias Algotsson , Philippe Busson , Nicolas Thevenin
发明人： Mattias Algotsson , Philippe Busson , Nicolas Thevenin
IPC分类号： C07C233/10
CPC分类号： C07C277/08 , C07C279/12
摘要： The present invention relates to a method for synthesis of an acrylamide derivative, starting with dissolving a salt of a nucleophilic amine in water to form an aqueous solution and desalting said solution with a base, comprising the following steps: a) addition of dissolved activated acrylic acid derivative to said solution; b) acidification of aqueous phase; and c) extraction of said aqueous phase.
摘要翻译：本发明涉及一种合成丙烯酰胺衍生物的方法，首先将亲核胺的盐溶解在水中形成水溶液，并用碱脱盐所述溶液，包括以下步骤：a）加入溶解的活化丙烯酸酸衍生物至所述溶液; b）水相酸化; 和c）提取所述水相。

16. 发明申请

US20070238088A1 Hydrophilic functionalized colloidal silica compositions, methods of making, and uses therefor 审中-公开
标题翻译：亲水官能化胶体二氧化硅组合物，制备方法和用途
公开(公告)号：US20070238088A1
公开(公告)日：2007-10-11
申请号：US11392133
申请日：2006-03-29
申请人： Slawomir Rubinsztajn , David Demoulpied , Omayra de Jesus , Gregory Goddard , James Van Alstine , Jan Larsson , Mohan Amaratunga , Nicolas Thevenin
发明人： Slawomir Rubinsztajn , David Demoulpied , Omayra de Jesus , Gregory Goddard , James Van Alstine , Jan Larsson , Mohan Amaratunga , Nicolas Thevenin
IPC分类号： C12N5/08 , C12Q1/00 , C04B14/04
CPC分类号： C09C1/3081 , B01D2221/10 , B01J13/003 , B01J13/0034 , B01J13/0047 , B82Y30/00 , C01P2004/62 , C01P2004/64
摘要： Disclosed are hydrophilic functionalized silica compositions that are stable and do not show significant pH increases upon heat sterilization. Also provided are methods to make hydrophilic functionalized silica compositions by reacting acidic silica particles with hydrophilic organosilanes. Further provided are methods of separating components in a mixture using hydrophilic functionalized silica compositions.
摘要翻译：公开了稳定的并且在加热灭菌时不显示显着的pH增加的亲水性官能化二氧化硅组合物。还提供了通过使酸性二氧化硅颗粒与亲水性有机硅烷反应来制备亲水性官能化二氧化硅组合物的方法。还提供了使用亲水性官能化二氧化硅组合物分离混合物中的组分的方法。

17. 发明申请

US20070112178A1 Antibody purification 有权
标题翻译：抗体纯化
公开(公告)号：US20070112178A1
公开(公告)日：2007-05-17
申请号：US10589718
申请日：2005-02-24
申请人： Bo-Lennart Johansson , Hans Johansson , Anders Ljunglof , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Bo-Lennart Johansson , Hans Johansson , Anders Ljunglof , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： C07K16/00 , C02F1/28
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01D15/3847 , B01J39/07 , B01J39/26 , B01J47/014 , C07K1/165 , B01D15/327
摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
摘要翻译：本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统。在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。

18. 发明申请

US20060188931A1 Method of preparing affinity ligands 失效
标题翻译：制备亲和配体的方法
公开(公告)号：US20060188931A1
公开(公告)日：2006-08-24
申请号：US10547569
申请日：2004-03-05
申请人： Jean-Luc Maloisel , Nicolas Thevenin
发明人： Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： C40B40/02 , C07F5/00
CPC分类号： B01J20/286 , B01D15/3804 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3255 , B01J20/3259
摘要： The present invention is a method of preparing affinity ligands, which method comprises to provide a cyclic scaffold comprising a thiol group, a carbonyl group and an amine group; to derivatise the amine group of said scaffold with an electrophile that carries either a functionality capable of affinity interaction or a functionality capable of a second interaction with a target molecule; and to open up the derivatised scaffold and add an amine that carries either a functionality capable of a second interaction or a functionality capable of affinity interaction with a target molecule. The method provides provide affinity ligands, which are capable of affinity interaction as well as a second kind of interaction.
摘要翻译：本发明是制备亲和配体的方法，该方法包括提供包含硫醇基，羰基和胺基的环状支架; 用携带亲和力相互作用的官能团或能够与靶分子进行第二次相互作用的官能团的亲电子试剂衍生所述支架的胺基团; 并打开衍生的支架并加入携带能够进行第二相互作用的官能团或能够与靶分子亲和相互作用的官能团的胺。该方法提供了提供亲和力配体，其能够进行亲和力相互作用以及第二种相互作用。

19. 发明授权

US07067059B2 Generation of ion exchanger media 有权
标题翻译：生成离子交换介质
公开(公告)号：US07067059B2
公开(公告)日：2006-06-27
申请号：US10489468
申请日：2002-09-12
申请人： Jean-Luc Maloisel , Nicolas Thevenin
发明人： Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： B01D15/08
CPC分类号： B01J20/3212 , B01J20/3204 , B01J20/321 , B01J20/3219 , B01J39/17 , B01J39/18 , B01J39/26
摘要： The present invention relates to a method of generating a separation medium comprising mixed mode cation-exchanger ligands coupled to a base matrix, which method comprises to provide a scaffold comprising a functional group and exhibiting a cyclic core structure; derivatise the scaffold with a reagent comprising a reactive group coupled to a residue R by reacting the functional group of the scaffold with said reactive group; open the cyclic structure of the resulting derivative; and react the product with a base matrix comprising a reactive group. The scaffold presents at least two functionalities; one sulphur-comprising group for coupling to the base matrix and one group that can be transformed into an ionic group.
摘要翻译：本发明涉及一种产生分离介质的方法，该分离介质包含与基础基质偶联的混合模式阳离子交换配体，该方法包括提供包含官能团并显示环状核心结构的支架; 通过使支架的官能团与所述反应性基团反应，用包含与残基R偶联的反应性基团的试剂衍生支架; 打开所得衍生物的环状结构; 并使产物与包含反应性基团的基质基质反应。脚手架至少有两个功能; 一个用于与基础基质偶联的含硫基团和一个可以转化成离子基团的基团。

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