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    • 20. 发明授权
    • Method of producing grafts
    • 生产移植物的方法
    • US5120833A
    • 1992-06-09
    • US669920
    • 1991-03-15
    • Alexander Kaplan
    • Alexander Kaplan
    • A61K38/00C07K14/78
    • C07K14/78A61K38/00Y10S514/801Y10S514/953
    • A unique method (10) for preparing protein-impacted grafts (22) wherein an untreated graft (22) is impacted and impregnated with a protein solution, ideally a collagen suspension (14) utilizing a dynamic flow soaking process (24). After soaking in the dynamic flow (24), the collagen-impacted graft is thermally and chemically fixed (26). Following a water wash (30) and glycine wash (34), the graft (22) is softened with a plastification procedure (36), after which it is air dried (38) and placed in a package and sterilized. With the method of the present invention, storable, surgically-ready collagen-impacted grafts can be reliably and rapidly prepared with a high degree of control and uniformity of characteristics in each graft than has heretofore been possible.
    • 用于制备受蛋白质影响的移植物(22)的独特方法(10),其中未处理的接枝物(22)被蛋白质溶液冲击并浸渍,理想地是使用动态流动浸泡方法(24)的胶原悬浮液(14)。 在动态流动(24)中浸泡后,胶原蛋白受影响的移植物被热和化学固定(26)。 在水洗(30)和甘氨酸洗涤(34)之后,移植物(22)用塑化程序(36)软化,然后将其风干(38)并置于包装中并灭菌。 利用本发明的方法,可以以比迄今为止可能的高度控制和每个移植物的特性均匀性可靠和快速地制备可手术的手术就绪的胶原蛋白的移植物。