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11. 发明授权

US06153188A Glycosylation variants of iduronate 2-sulfatase 有权
公开(公告)号：US06153188A
公开(公告)日：2000-11-28
申请号：US249003
申请日：1999-02-12
申请人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
发明人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
IPC分类号： A61K38/00 , C12N5/18 , C12N5/22 , C12N9/16 , A61K38/46
CPC分类号： C12N9/16 , A61K38/00
摘要： The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present invention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.

12. 发明授权

US6149909A Synthetic .alpha.-L-iduronidase and genetic sequences encoding same 有权
标题翻译：合成的α-L-异构酶和编码相同的遗传序列
公开(公告)号：US6149909A
公开(公告)日：2000-11-21
申请号：US191171
申请日：1998-11-13
申请人： Hamish Steele Scott , Donald Stewart Anson , Annette Marie Orsborn , Paul Victor Nelson , Peter Roy Clements , Charles Phillip Morris , John Joseph Hopwood
发明人： Hamish Steele Scott , Donald Stewart Anson , Annette Marie Orsborn , Paul Victor Nelson , Peter Roy Clements , Charles Phillip Morris , John Joseph Hopwood
IPC分类号： A61K38/00 , C12N9/24 , A61K38/47
CPC分类号： C12Y302/01076 , C12N9/2402 , A61K38/00 , C07K2319/00
摘要： The present invention relates generally to .alpha.-L-iduronidase and to genetic sequences encoding same. More particularly, the present invention provides an isolated nucleic acid molecule comprising a sequence of nucleotides which encodes or are complementary to a sequence which encodes a mammalian .alpha.-L-iduronidase or fragment or derivative thereof and to the recombinant enzyme encoded thereby. These molecules are useful in the investigation, diagnosis and treatment of subjects suspected of or suffering from .alpha.-L-iduronidase deficiency.
摘要翻译：本发明一般涉及α-L-艾杜糖醛酸酶和编码其的遗传序列。更具体地，本发明提供了分离的核酸分子，其包含编码或与编码哺乳动物α-L-胆碱蛋白酶或其片段或衍生物的序列和与其编码的重组酶的序列互补的核苷酸序列。这些分子可用于疑似或遭受α-L-神经氨酸酶缺陷的受试者的调查，诊断和治疗。

13. 发明授权

US5932211A Glycosylation variants of iduronate 2-sulfatase 失效
标题翻译：糖尿病糖苷酸2-硫酸酯酶的糖基化变体
公开(公告)号：US5932211A
公开(公告)日：1999-08-03
申请号：US345212
申请日：1994-11-28
申请人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
发明人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
IPC分类号： A61K38/00 , C12N5/18 , C12N5/22 , C12N9/16 , A61K38/46
CPC分类号： C12N9/16 , A61K38/00
摘要： The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present intention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.
摘要翻译：本发明提供了高度糖基化的糖尿病2-硫酸酯酶，其包含与天然来源例如纯化的伊曲膦酸酯-2-硫酸酯酶相比，具有至少5千道尔顿（kDa）多糖的伊万二酸-2-硫酸酯酶多肽。人肝。本发明还提供了这样一种高度糖基化的阿魏酸-2-硫酸酯酶的酶活性多肽片段或变体。本发明还提供了编码艾杜糖醛酸-2-硫酸酯酶的分离的核酸，以及表达载体，宿主细胞和用于生产本发明的高度糖基化的糖尿病2-硫酸酯酶的方法。在一个实施方案中，本发明涉及一种生产糖基化的阿魏酸-2-硫酸酯酶的方法，其包括培养含有编码酶活性的阿魏酸-2-硫酸酯酶多肽的核酸的宿主细胞，其中宿主细胞将多肽糖基化成比由天然人肝细胞表达的天然异二烯酸2-硫酸酯酶多肽更大程度。

14. 发明授权

US5798239A Glycosylation variants of iduronate 2-sulfatase 失效
公开(公告)号：US5798239A
公开(公告)日：1998-08-25
申请号：US484494
申请日：1995-06-07
申请人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
发明人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
IPC分类号： A61K38/00 , C12N5/18 , C12N5/22 , C12N9/16 , A61K38/46 , A61K38/47 , C12N9/00
CPC分类号： C12N9/16 , A61K38/00
摘要： The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present invention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.

15. 发明申请

US20090291432A1 Genetic profiles associated with the 957C>T polymorphism in the DRD2 gene 审中-公开
标题翻译：与DRD2基因中957C> T多态性相关的遗传谱
公开(公告)号：US20090291432A1
公开(公告)日：2009-11-26
申请号：US11628397
申请日：2005-06-01
申请人： Charles Phillip Morris , Angela Van Daal , Christopher Dean Swagell , Bruce Robert Lawford , Ross McDonald Young
发明人： Charles Phillip Morris , Angela Van Daal , Christopher Dean Swagell , Bruce Robert Lawford , Ross McDonald Young
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6883 , C12Q2600/136 , C12Q2600/156 , C12Q2600/158 , C12Q2600/172 , G01N2800/28 , G01N2800/30
摘要： The present invention relates to a method for profiling an individual or group of individuals with respect to a neurological, psychiatric or psychological condition, phenotype or state, including a sub-threshold neurological, psychiatric or psychological condition, phenotype or state. More particularly, the present invention identifies a genetic profile associated with the 957C>T polymorphysm within the dopamine receptor D2 (DRD2), indicating a predisposition to schizophrenia and other neurological diseases.
摘要翻译：本发明涉及一种关于神经，精神或心理状况，表型或状态（包括亚阈值神经，精神或心理状况，表型或状态）的个体或个体个体的分析方法。更具体地，本发明鉴定与多巴胺受体D2（DRD2）内的957C> T多态性相关的遗传谱，表明精神分裂症和其他神经疾病的倾向。

16. 发明授权

US6017738A Solid phase amplification process 失效
标题翻译：固相扩增工艺
公开(公告)号：US6017738A
公开(公告)日：2000-01-25
申请号：US761862
申请日：1996-12-09
申请人： Charles Phillip Morris , Raymond John Harris
发明人： Charles Phillip Morris , Raymond John Harris
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6834
摘要： A method for detecting a target nucleic acid sequence comprises: (a) providing a first primer hybridizing to the target nucleic acid sequence, wherein the primer is immobilized on an immobile solid phase support by a direct chemical linkage between the primer and the solid phase support, wherein the solid phase support forms a part of or is insertable into a container for a sample to be tested, (b) providing a second primer hybridizing to the target nucleic acid sequence in the opposite direction, wherein the second primer is labelled with a detectable label, (c) reacting the first and second primers with a sample containing nucleic acid sequences under conditions which allow amplification of the nucleic acid sequences that hybridize to the first and second primers in the container for the sample, and (d) detecting the presence of bound second primer. Alternatively, the label on the second primer can be attached or incorporated either during or after the amplification process. An assay system or kit for use in this method includes a first primer hybridizing to the target nucleic acid sequence, a second primer hybridizing to the target nucleic acid sequence in the opposite direction, and reagents for amplification of the sample containing nucleic acid sequences under conditions which allow amplification of the nucleic acid sequences that hybridize to the first and second primers in the container for the sample, and reagents for detection of the label on the bound second primer.
摘要翻译：用于检测靶核酸序列的方法包括：（a）提供与靶核酸序列杂交的第一引物，其中通过引物和固相载体之间的直接化学键将引物固定在固定的固相载体上其中所述固相载体形成待测试样品的一部分或可插入容器中，（b）提供与所述靶核酸序列沿相反方向杂交的第二引物，其中所述第二引物用（c）使第一和第二引物与含有核酸序列的样品在允许扩增与样品容器中的第一和第二引物杂交的核酸序列的条件下反应，和（d）检测存在结合的第二引物。或者，第二引物上的标记可以在扩增过程中或之后附着或并入。用于该方法的测定系统或试剂盒包括与靶核酸序列杂交的第一引物，与相反方向与靶核酸序列杂交的第二引物和用于在条件下扩增含有核酸序列的样品的试剂其允许扩增与样品容器中的第一和第二引物杂交的核酸序列，以及用于检测结合的第二引物上的标记的试剂。

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