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131. 发明申请

US20130007923A1 USE OF Cry1Da IN COMBINATION WITH Cry1Ca FOR MANAGEMENT OF RESISTANT INSECTS 有权
标题翻译：使用Cry1Da与Cry1Ca联合治疗耐药性
公开(公告)号：US20130007923A1
公开(公告)日：2013-01-03
申请号：US13516663
申请日：2010-12-16
申请人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Aaron T. Woosley , Stephanie L. Burton
发明人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Aaron T. Woosley , Stephanie L. Burton
IPC分类号： A01H5/00 , C12N5/10 , A01C11/00 , A01N37/46 , A01G1/00 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , Y02A40/162 , A01N65/00
摘要： The subject invention includes methods and plants for controlling fall armyworm lepidopteran insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Ca insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.
摘要翻译：本发明包括用于控制秋季粘虫鳞翅目昆虫的方法和植物，所述植物包含Cry1Da杀虫蛋白和Cry1Ca杀虫蛋白，以及包含该对蛋白质的其它蛋白质的各种组合，以延迟或预防昆虫的抗性发展。

132. 发明申请

US20120322660A1 DOWN-REGULATING GENE EXPRESSION IN INSECT PESTS 有权
标题翻译：下调调控基因表达
公开(公告)号：US20120322660A1
公开(公告)日：2012-12-20
申请号：US13462636
申请日：2012-05-02
申请人： Myriam Beghyn , Thierry Andre Olivier Eddy Bogaert , Pascale Feldmann , Romaan Raemaekers
发明人： Myriam Beghyn , Thierry Andre Olivier Eddy Bogaert , Pascale Feldmann , Romaan Raemaekers
IPC分类号： A01N57/16 , C12N15/11 , C12N15/63 , A01P13/00 , A01P7/04 , A01N63/00 , A01N37/46 , C07H21/02 , C12N1/21
CPC分类号： C12N15/113 , A01N57/16 , A01N63/00 , C07K14/43563 , C12N15/8218 , C12N15/8286 , C12N2310/14 , Y02A40/162
摘要： The present invention relates to genetic control of infestation by insect pest species, particularly prevention and/or control of pest infestation of plants, using interfering ribonucleic acid (RNA) molecules. Compositions and combinations containing the interfering RNA molecules of the invention for use in topical applications, for example in the form of insecticides.
摘要翻译：本发明涉及使用干扰核糖核酸（RNA）分子的昆虫害虫侵染的遗传控制，特别是预防和/或控制植物害虫侵染。含有用于局部应用的本发明的干扰RNA分子的组合物和组合，例如以杀虫剂的形式。

133. 发明申请

US20120311745A1 COMBINED USE OF CRY1CA AND CRY1AB PROTEINS FOR INSECT RESISTANCE MANAGEMENT 有权
标题翻译：联合使用CRY1CA和CRY1AB蛋白进行耐药性管理
公开(公告)号：US20120311745A1
公开(公告)日：2012-12-06
申请号：US13516615
申请日：2010-12-16
申请人： Thomas Meade , Aaron T. Woosley , Kenneth Narva , Stephanie L. Burton , Nicholas P. Storer , Joel J. Sheets
发明人： Thomas Meade , Aaron T. Woosley , Kenneth Narva , Stephanie L. Burton , Nicholas P. Storer , Joel J. Sheets
IPC分类号： A01H5/00 , C12N5/10 , C12N1/21 , C12N1/19 , A01C11/00 , A01N63/04 , A01N63/00 , A01P7/04 , A01G1/00 , A01H5/10 , C12N1/13
CPC分类号： C12N15/8286 , A01N63/02 , Y02A40/162 , A01N25/00
摘要： The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising Cry1Ca insecticidal protein and a Cry1Ab insecticidal protein in combination to delay or prevent development of resistance by the insects.
摘要翻译：本发明包括用于控制鳞翅目昆虫的方法和植物，所述植物包含Cry1Ca杀虫蛋白和Cry1Ab杀虫蛋白，以延迟或预防昆虫的抗性发展。

134. 发明申请

US20120311743A1 SOYBEAN TRANSFORMATION USING HPPD INHIBITORS AS SELECTION AGENTS 审中-公开
标题翻译：使用HPPD抑制剂作为选择剂的大豆转化
公开(公告)号：US20120311743A1
公开(公告)日：2012-12-06
申请号：US13576605
申请日：2011-02-01
申请人： Flavie Coulombier , Hélène Eckert , Yannick Favre , Bernard Pelissier
发明人： Flavie Coulombier , Hélène Eckert , Yannick Favre , Bernard Pelissier
IPC分类号： A01H1/06 , C12N5/10 , C12N15/82
CPC分类号： C12N15/8209 , C12N9/0069 , C12N15/8205 , C12N15/8212 , C12N15/8274 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： The invention relates to methods for Agrobacterium-mediated transformation of soybean organogenic tissue using a gene or genes for tolerance to HPPD inhibitors as selection marker. The invention also relates to methods for regenerating transgenic soybean plants from said transformed soybean cells or tissue, and to transgenic soybean plants and seeds obtained by such methods.
摘要翻译：本发明涉及农杆菌介导的用HPPD抑制剂作为选择标记的基因或基因对大豆器官发生组织的转化的方法。本发明还涉及从所述转化的大豆细胞或组织再生转基因大豆植物以及通过这些方法获得的转基因大豆植物和种子的方法。

135. 发明授权

US08319019B2 Bacillus thuringiensis gene with lepidopteran activity 有权
公开(公告)号：US08319019B2
公开(公告)日：2012-11-27
申请号：US12616298
申请日：2009-11-11
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
IPC分类号： A01H5/00 , A01H5/10 , C07K14/325 , A01N37/18 , C12N15/32 , C12N1/21 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

136. 发明授权

US08314290B2 Temporal regulation of gene expression by MicroRNAs 有权
标题翻译： MicroRNAs基因表达的时间调控
公开(公告)号：US08314290B2
公开(公告)日：2012-11-20
申请号：US11768264
申请日：2007-06-26
申请人： Edwards Allen , Sara Elizabeth Heisel , Sergey Ivashuta , Elysia Katherine Krieger , Jennifer Lynn Lutke , Robert Joseph Meister , Yuanji Zhang
发明人： Edwards Allen , Sara Elizabeth Heisel , Sergey Ivashuta , Elysia Katherine Krieger , Jennifer Lynn Lutke , Robert Joseph Meister , Yuanji Zhang
IPC分类号： C12N15/29 , C12N15/82 , C12N5/04 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8218 , C12N15/8216 , C12N15/8217 , C12N15/8225 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
摘要： This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
摘要翻译：本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。更具体地，本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因，其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞，植物和种子。

137. 发明申请

US20120283111A1 Bacterial mRNA screen strategy for novel pesticide-encoding nucleic acid molecule discovery 审中-公开
标题翻译：新型农药编码核酸分子发现的细菌mRNA筛选策略
公开(公告)号：US20120283111A1
公开(公告)日：2012-11-08
申请号：US13459280
申请日：2012-04-30
申请人： Andre R. Abad , Deirdre M. Kapka-Kitzman
发明人： Andre R. Abad , Deirdre M. Kapka-Kitzman
IPC分类号： C40B30/00 , C12Q1/68
CPC分类号： C12N15/8286 , C12N15/1072 , C12N15/8279 , Y02A40/162
摘要： Methods are provided for isolating and identifying novel pesticide-encoding nucleic acid molecules from bacterial strains identified as having a pesticidal polypeptide. The methods involve selecting a bacterial strain having or suspected of having at least one pesticide-encoding plasmid, curing the at least one pesticide-encoding plasmid from the bacterial strain and performing a subtractive hybridization to obtain plasmid mRNA encoding the pesticidal polypeptide. The plasmid mRNA can be used to make a cDNA library from which the pesticidal polypeptide can be isolated and subsequently identified.
摘要翻译：提供了从被鉴定为具有杀虫多肽的细菌菌株中分离和鉴定新型农药编码核酸分子的方法。所述方法包括选择具有或怀疑具有至少一种农药编码质粒的细菌菌株，从细菌菌株固化至少一种农药编码质粒，并进行消减杂交以获得编码杀虫多肽的质粒mRNA。质粒mRNA可用于制备可从其中分离并随后鉴定的杀虫多肽的cDNA文库。

138. 发明授权

US08304604B2 DIG-3 insecticidal Cry toxins 有权
标题翻译： DIG-3杀虫剂Cry毒素
公开(公告)号：US08304604B2
公开(公告)日：2012-11-06
申请号：US12730295
申请日：2010-03-24
申请人： Justin M. Lira , Holly J. Butler , Doug A. Smith , Kenneth Narva , Thomas Meade
发明人： Justin M. Lira , Holly J. Butler , Doug A. Smith , Kenneth Narva , Thomas Meade
IPC分类号： A01H5/00 , A01N57/18 , C12N15/63 , C12N15/82 , C12N15/32 , C07K14/325
CPC分类号： A01N43/50 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： DIG-3 Cry toxins, polynucleotides encoding such toxins, and transgenic plants that produce such toxins are useful to control insect pests.
摘要翻译： DIG-3 Cry毒素，编码这种毒素的多核苷酸和产生这种毒素的转基因植物可用于防治害虫。

139. 发明申请

US20120260372A1 TRANSGENIC PLANTS EXPRESSING MODIFIED Cry3A 有权
标题翻译：表达改性Cry3A的转基因植物
公开(公告)号：US20120260372A1
公开(公告)日：2012-10-11
申请号：US13525413
申请日：2012-06-18
申请人： Eric CHEN , Cheryl Stacy
发明人： Eric CHEN , Cheryl Stacy
IPC分类号： A01H5/00 , A01H5/10
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for controlling plant pests are disclosed. In particular, transgenic maize plants comprising nucleic acid molecules encoding modified Cry3A toxins having increased toxicity to western corn rootworm are provided. Such modified Cry3A toxins, into which a non-naturally occurring protease recognition site is inserted, are expressed at levels in transgenic plants to inhibit insect feeding damage and cause insect mortality.
摘要翻译：公开了用于控制植物害虫的组合物和方法。特别地，提供了包含对西方玉米根虫具有增加的毒性的编码修饰的Cry3A毒素的核酸分子的转基因玉米植物。其中插入非天然存在的蛋白酶识别位点的这种经修饰的Cry3A毒素以转基因植物的水平表达，以抑制昆虫进食损伤并引起昆虫死亡。

140. 发明授权

US08216806B2 Modified Cry3A toxins and nucleic acid sequences coding therefor 有权
标题翻译：修饰的Cry3A毒素及其编码的核酸序列
公开(公告)号：US08216806B2
公开(公告)日：2012-07-10
申请号：US13189888
申请日：2011-07-25
申请人： Eric Chen , Cheryl Stacy
发明人： Eric Chen , Cheryl Stacy
IPC分类号： C12P21/06 , C07H21/02 , C07K14/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Methods for making a modified Cry3A toxin are disclosed. Such methods include the insertion of a protease recognition site that is recognized by a gut protease of a target insect, such as corn rootworm, into at least one position of a Cry3A toxin so that a modified Cry3A toxin is thus designed. The coding sequence of the modified Cry3A toxin may be transformed into a host cell and the host cell grown under conditions that allow the host cell to produce the modified Cry3A toxin. The host cell may be a plant cell and the plant may be comprised in a transgenic plant. Thus, the transgenic plant may be used to produce the modified Cry3A toxin.
摘要翻译：公开了制备修饰的Cry3A毒素的方法。这样的方法包括将目标昆虫（例如玉米根虫）的肠蛋白酶识别的蛋白酶识别位点插入Cry3A毒素的至少一个位置，从而设计出修饰的Cry3A毒素。修饰的Cry3A毒素的编码序列可以转化到宿主细胞中，宿主细胞在允许宿主细胞产生修饰的Cry3A毒素的条件下生长。宿主细胞可以是植物细胞，并且植物可以包含在转基因植物中。因此，转基因植物可用于产生经修饰的Cry3A毒素。

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