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1. 发明申请

US20120283111A1 Bacterial mRNA screen strategy for novel pesticide-encoding nucleic acid molecule discovery 审中-公开
标题翻译：新型农药编码核酸分子发现的细菌mRNA筛选策略
公开(公告)号：US20120283111A1
公开(公告)日：2012-11-08
申请号：US13459280
申请日：2012-04-30
申请人： Andre R. Abad , Deirdre M. Kapka-Kitzman
发明人： Andre R. Abad , Deirdre M. Kapka-Kitzman
IPC分类号： C40B30/00 , C12Q1/68
CPC分类号： C12N15/8286 , C12N15/1072 , C12N15/8279 , Y02A40/162
摘要： Methods are provided for isolating and identifying novel pesticide-encoding nucleic acid molecules from bacterial strains identified as having a pesticidal polypeptide. The methods involve selecting a bacterial strain having or suspected of having at least one pesticide-encoding plasmid, curing the at least one pesticide-encoding plasmid from the bacterial strain and performing a subtractive hybridization to obtain plasmid mRNA encoding the pesticidal polypeptide. The plasmid mRNA can be used to make a cDNA library from which the pesticidal polypeptide can be isolated and subsequently identified.
摘要翻译：提供了从被鉴定为具有杀虫多肽的细菌菌株中分离和鉴定新型农药编码核酸分子的方法。所述方法包括选择具有或怀疑具有至少一种农药编码质粒的细菌菌株，从细菌菌株固化至少一种农药编码质粒，并进行消减杂交以获得编码杀虫多肽的质粒mRNA。质粒mRNA可用于制备可从其中分离并随后鉴定的杀虫多肽的cDNA文库。

2. 发明申请

US20120297503A1 Novel Bacillus thuringiensis Gene with Lepidopteran Activity 有权
公开(公告)号：US20120297503A1
公开(公告)日：2012-11-22
申请号：US13555393
申请日：2012-07-23
申请人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
IPC分类号： C07K14/32 , C12N1/21 , C12N5/10 , A01H1/06 , A01N37/46 , A01P7/04 , C12P21/02 , A01H5/00 , C12N15/31 , A01H5/10
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

3. 发明申请

US20100190707A1 Novel Bacillus Thuringiensis Gene with Lepidopteran Activity 有权
公开(公告)号：US20100190707A1
公开(公告)日：2010-07-29
申请号：US12652780
申请日：2010-01-06
申请人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
IPC分类号： A61K38/16 , C12N15/31 , C12N5/10 , A01H5/00 , C12N1/21 , C07K14/00 , C12P21/06
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

4. 发明授权

US08252972B2 Bacillus thuringiensis gene with lepidopteran activity 有权
标题翻译：具有鳞翅目昆虫活性的苏云金芽孢杆菌基因
公开(公告)号：US08252972B2
公开(公告)日：2012-08-28
申请号：US12652780
申请日：2010-01-06
申请人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
IPC分类号： C12N15/82 , C12N15/32 , C12N5/10 , C12N1/21 , C12P21/02 , A01H5/00 , A01H5/10
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的昆虫的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其包括鳞翅目昆虫害虫。本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，DNA构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

5. 发明授权

US08692065B2 Bacillus thuringiensis gene with lepidopteran activity 有权
公开(公告)号：US08692065B2
公开(公告)日：2014-04-08
申请号：US12628239
申请日：2009-12-01
申请人： Andre R. Abad , Deirdre M. Kapka-Kitzman , John P. Mathis , Thomas C. Wolfe
发明人： Andre R. Abad , Deirdre M. Kapka-Kitzman , John P. Mathis , Thomas C. Wolfe
IPC分类号： C12N15/32 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8286 , A01N63/02 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

6. 发明授权

US08586832B2 Bacillus thuringiensis gene with Lepidopteran activity 有权
标题翻译：具有鳞翅目昆虫活性的苏云金芽孢杆菌基因
公开(公告)号：US08586832B2
公开(公告)日：2013-11-19
申请号：US12962817
申请日：2010-12-08
申请人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
IPC分类号： C12N15/82 , C12N15/32 , C12N15/52 , C12N15/74 , C12N5/04 , C12N5/10 , C12N1/21 , A01H5/00 , A01H5/10 , A01N37/18
CPC分类号： C07K14/325 , A61K38/00 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的昆虫的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其包括鳞翅目昆虫害虫。本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，DNA构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

7. 发明申请

US20100192256A1 Novel Bacillus Thuringiensis Gene with Lepidopteran Activity 有权
公开(公告)号：US20100192256A1
公开(公告)日：2010-07-29
申请号：US12628239
申请日：2009-12-01
申请人： Andre R. Abad , Deirdre M. Kapka-Kitzman , John P. Mathis , Thomas C. Wolfe
发明人： Andre R. Abad , Deirdre M. Kapka-Kitzman , John P. Mathis , Thomas C. Wolfe
IPC分类号： A01H5/00 , C07H21/04 , C12N5/10 , C07K14/32 , C12N15/82 , C12N15/63 , C12N1/21 , A61K38/16 , C12P21/06
CPC分类号： C12N15/8286 , A01N63/02 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

8. 发明授权

US08878007B2 Bacillus thuringiensis gene with lepidopteran activity 有权
公开(公告)号：US08878007B2
公开(公告)日：2014-11-04
申请号：US13413707
申请日：2012-03-07
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi , Thomas C. Wolfe
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi , Thomas C. Wolfe
IPC分类号： A01H5/00 , C12N15/82 , C12P1/00 , C07K1/00 , C07H21/02 , A01N63/02 , C07K14/325
CPC分类号： C12N15/8279 , A01N63/02 , C07K14/325
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

9. 发明授权

US08319019B2 Bacillus thuringiensis gene with lepidopteran activity 有权
公开(公告)号：US08319019B2
公开(公告)日：2012-11-27
申请号：US12616298
申请日：2009-11-11
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
IPC分类号： A01H5/00 , A01H5/10 , C07K14/325 , A01N37/18 , C12N15/32 , C12N1/21 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

10. 发明授权

US08148077B2 Method for identifying novel genes 有权
标题翻译：识别新基因的方法
公开(公告)号：US08148077B2
公开(公告)日：2012-04-03
申请号：US11780884
申请日：2007-07-20
申请人： Andre R. Abad , Hua Dong , Susan B. Lo , Billy F. McCutchen , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Susan B. Lo , Billy F. McCutchen , Xiaomei Shi
IPC分类号： C12P19/34
CPC分类号： C12N15/8286 , C12Q1/6813 , Y02A40/162 , C12Q2531/113
摘要： Methods and compositions for identifying novel genes that share regions of homology with known genes from target groups of genes of interest are provided. The methods comprise systematically designing oligonucleotide primers that are specific for regions of homology within the nucleotide sequences of a target group of known genes and performing successive rounds of PCR amplification of nucleic acid material from an organism of interest. The PCR steps are intended to identify and amplify nucleic acids comprising both known and novel genes. Nucleic acid molecules comprising known genes are detected and eliminated from further consideration by dot blot analysis using oligonucleotide probes specific for the known genes in the target group. Potentially novel genes are subjected to further sequence analysis to confirm novelty and assayed for biological activity. Compositions of the present invention include novel polynucleotides, and variants and fragments thereof, that comprise novel genes and the polypeptides encoded thereby.
摘要翻译：提供了用于鉴定与目标基因组的已知基因具有同源性区域的新基因的方法和组合物。所述方法包括系统地设计对已知基因的靶组的核苷酸序列内的同源区特异性的寡核苷酸引物，并且从感兴趣的生物体进行连续循环的核酸材料的PCR扩增。 PCR步骤旨在鉴定和扩增包含已知和新基因的核酸。通过使用针对靶组中已知基因特异的寡核苷酸探针的斑点印迹分析，进一步考虑检测并消除包含已知基因的核酸分子。将潜在的新基因进行进一步的序列分析以证实新颖性并测定生物活性。本发明的组合物包括新型多核苷酸及其变体和片段，其包含新基因和由其编码的多肽。

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