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131. 发明申请

US20030082571A1 Linear nucleic acid and sequence therefor 审中-公开
标题翻译：线性核酸及其序列
公开(公告)号：US20030082571A1
公开(公告)日：2003-05-01
申请号：US10117108
申请日：2002-04-08
发明人： Edward Hanna Kachab , Graeme Ross Barnett
IPC分类号： C12Q001/68 , C07H021/04
CPC分类号： C12Q1/6876 , C12Q1/6832 , C12Q1/6837
摘要： Nucleic acids and sequences therefor are disclosed that are characterized by a reduction or lack of internal secondary structure, are capable of hybridizing with a complementary nucleic acid and do not hybridize with non-complementary nucleic acids (eg. do not cross-hybridize or form dimers) under low stringency hybridization conditions. In particular, the nucleotide sequences enable use of these nucleic acids, without reduction in target hybridization efficiency with increasing nucleic acid length. The nucleic acids may be used with analyte capture systems, for example medical, veterinary and agricultural diagnostic applications. In particular, the nucleic acid may be used as irrelevant binding pairs in an analyte capture system, such as an array or lateral flow assay.
摘要翻译：公开的核酸及其序列的特征在于还原或缺乏内部二级结构，能够与互补核酸杂交并且不与非互补核酸杂交（例如不杂交或形成二聚体）在低严格杂交条件下。特别地，核苷酸序列能够使用这些核酸，而不降低目标杂交效率并增加核酸长度。核酸可以与分析物捕获系统一起使用，例如医学，兽医和农业诊断应用。特别地，核酸可以用作分析物捕获系统中的不相关的结合对，例如阵列或侧向流动测定。

132. 发明申请

US20030054542A1 Multiple ring assembly for providing specimen to reaction receptacles within an automated analyzer 有权
标题翻译：多个环组件，用于在自动分析仪内将反应容器提供样品
公开(公告)号：US20030054542A1
公开(公告)日：2003-03-20
申请号：US10268899
申请日：2002-10-11
发明人： Ralph E. Burns , Ernest V. Hansberry
IPC分类号： C12M001/34 , C12M001/00
CPC分类号： C12Q1/6834 , B01F9/0001 , B01F9/0014 , B01F9/0021 , B01F2009/0074 , B01L7/52 , B01L7/5255 , B01L9/06 , B01L2200/147 , B01L2300/1822 , B01L2300/1827 , B03C1/282 , B03C1/288 , B03C1/30 , B03C2201/26 , C12Q1/6813 , C12Q1/6832 , C12Q2537/10 , G01N35/0098 , G01N35/0099 , G01N35/025 , G01N35/1065 , G01N2035/00356 , G01N2035/00524 , G01N2035/0437 , G01N2035/0455 , G01N2035/103 , Y10T29/53657 , Y10T29/53683 , Y10T29/53843 , Y10T156/1105 , Y10T156/1906 , Y10T436/11 , Y10T436/111666 , Y10T436/113332 , Y10T436/114998 , Y10T436/119163 , Y10T436/2575
摘要： An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, preforming an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step.
摘要翻译：用于执行多个诊断测定的自动分析仪同时包括多个站或模块，其中对反应容器中包含的流体样品进行分析方面的分析。分析仪包括用于自动准备标本样品，在规定温度下规定时间孵育样品，预处理分析物分离程序以及确定目标分析物的存在的站。自动插座输送系统将反应容器从一个站移动到另一个站。分析仪还包括用于以机器可接近的方式携带多个样本管和一次性移液管尖端的装置，用于搅拌包含固体支持材料的悬浮液并用于提供用于机器通入的容器的靶捕获试剂的容器的装置，以及用于在温度受控的环境中容纳试剂容器的装置，并且提供用于机器通入的容器。执行自动诊断测定的方法包括用于分离和扩增靶分析物的自动化方法。该过程通过在站之间自动移动包含固体支持材料和流体样品的多个反应容器中的每一个来进行，用于孵育反应容器的内容物并将与固体支持物结合的目标分析物与流体样品分离。在分析物分离步骤之后和最终孵育步骤之前，将分离的分析物加入扩增试剂。

133. 发明申请

US20030054378A1 Method for detecting target nucleotide sequence 审中-公开
公开(公告)号：US20030054378A1
公开(公告)日：2003-03-20
申请号：US10103798
申请日：2002-03-25
申请人： Isao Karube
发明人： Isao Karube , Shinya Sawata , Ryohei Nagata
IPC分类号： C12Q001/68 , C12P019/34
CPC分类号： C12Q1/6832 , C12Q1/686 , C12Q2531/107 , C12Q2565/501
摘要： An object of the present invention is to provide a method for detecting a target nucleotide sequence using a complementary nucleotide sequence that has an excellent sensitivity of detection. The method comprises the steps of converting the target nucleotide sequence to a partially double-stranded nucleotide sequence which is double-stranded at one part and single-stranded in the remaining part and detecting said partially double stranded nucleotide sequence using a nucleotide sequence that is complementary to the target nucleotide sequence.

134. 发明申请

US20030049667A1 Apparatus and process for rapid hybridization 审中-公开
标题翻译：快速杂交的装置和方法
公开(公告)号：US20030049667A1
公开(公告)日：2003-03-13
申请号：US10217694
申请日：2002-08-13
发明人： Yang Wen-Tung , Lee Kan-Hung , Tsai Chuan-Mei , Shih Yu-Hau , Wang Yih-Weng
IPC分类号： C12Q001/68 , C12M001/34
CPC分类号： C12Q1/6832 , C12Q2565/125
摘要： The invention provides an apparatus for hybridization reaction which comprises a holder for loading a solid support and an electric field generator comprising a power controller and a first electrode plate and a second electrode plate respectively placed below or above the holder for generating a direction-convertible electric field to at least cover the solid support.
摘要翻译：本发明提供一种用于杂交反应的装置，其包括用于装载固体支持物的保持器和包括功率控制器和分别放置在保持器下方或上方的第一电极板和第二电极板的电场发生器，用于产生方向可转换电至少覆盖固体支撑。

135. 发明申请

US20030027206A1 Automated method for determining the presence of a target nucleic acid in a sample 有权
标题翻译：用于确定样品中靶核酸的存在的自动方法
公开(公告)号：US20030027206A1
公开(公告)日：2003-02-06
申请号：US10263939
申请日：2002-10-03
发明人： Kelly G. Ammann , Ralph E. Burns , Ernest V. Hansberry , Glenn A. Horner , Cheryl A. Jakub , John E. Kling , Donald J. Nieglos , Robert E. Schneider , Robert J. Smith
IPC分类号： C12Q001/68 , C12P019/34 , C12M001/34
CPC分类号： C12Q1/6834 , B01F9/0001 , B01F9/0014 , B01F9/0021 , B01F2009/0074 , B01L7/52 , B01L7/5255 , B01L9/06 , B01L2200/147 , B01L2300/1822 , B01L2300/1827 , B03C1/282 , B03C1/288 , B03C1/30 , B03C2201/26 , C12Q1/6813 , C12Q1/6832 , C12Q2537/10 , G01N35/0098 , G01N35/0099 , G01N35/025 , G01N35/1065 , G01N2035/00356 , G01N2035/00524 , G01N2035/0437 , G01N2035/0455 , G01N2035/103 , Y10T29/53657 , Y10T29/53683 , Y10T29/53843 , Y10T156/1105 , Y10T156/1906 , Y10T436/11 , Y10T436/111666 , Y10T436/113332 , Y10T436/114998 , Y10T436/119163 , Y10T436/2575
摘要： An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, preforming an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step.
摘要翻译：用于执行多个诊断测定的自动分析仪同时包括多个站或模块，其中对反应容器中包含的流体样品进行分析方面的分析。分析仪包括用于自动准备标本样品，在规定温度下规定时间孵育样品，预处理分析物分离程序以及确定目标分析物的存在的站。自动插座输送系统将反应容器从一个站移动到另一个站。分析仪还包括用于以机器可接近的方式携带多个样本管和一次性移液管尖端的装置，用于搅拌包含固体支持材料的悬浮液并用于提供用于机器通入的容器的靶捕获试剂的容器的装置，以及用于在温度受控的环境中容纳试剂容器的装置，并且提供用于机器通入的容器。执行自动诊断测定的方法包括用于分离和扩增靶分析物的自动化方法。该过程通过在站之间自动移动包含固体支持材料和流体样品的多个反应容器中的每一个来进行，用于孵育反应容器的内容物并将与固体支持物结合的目标分析物与流体样品分离。在分析物分离步骤之后和最终孵育步骤之前，将分离的分析物加入扩增试剂。

136. 发明申请

US20020164614A1 Method and kit for enhancing the association rates of polynucleotides 有权
标题翻译：用于增强多核苷酸结合速率的方法和试剂盒
公开(公告)号：US20020164614A1
公开(公告)日：2002-11-07
申请号：US10020596
申请日：2001-12-07
发明人： Michael M. Becker
IPC分类号： C12Q001/70 , C12Q001/68
CPC分类号： C12Q1/6818 , C12Q1/6832 , C12Q1/6839 , C12Q1/70 , C12Q2527/125
摘要： The present invention features a method and associated kit for increasing the association rate between polynucleotides having complementary, single-stranded regions, where the method includes providing to a test sample containing the complementary polynucleotides one or more synthetic, water soluble polycationic polymers.
摘要翻译：本发明的特征在于一种用于增加具有互补的单链区域的多核苷酸之间的缔合速率的方法和相关试剂盒，其中所述方法包括向含有互补多核苷酸的测试样品提供一种或多种合成的水溶性聚阳离子聚合物。

137. 发明授权

US06475721B2 Sequence specific detection of nucleic acids using a solid carrier bound with nucleic acid analog probes 失效
标题翻译：使用与核酸模拟探针结合的固体载体对核酸进行序列特异性检测
公开(公告)号：US06475721B2
公开(公告)日：2002-11-05
申请号：US08894808
申请日：1997-11-12
申请人： Jörg Kleiber , Henrik Ørum , Ane-Ullerup Lester , Albert Geiger
发明人： Jörg Kleiber , Henrik Ørum , Ane-Ullerup Lester , Albert Geiger
IPC分类号： C12Q168
CPC分类号： C12Q1/6837 , C12Q1/6813 , C12Q1/6827 , C12Q1/6832
摘要： A method for the detection of point mutation and polymorphisms in nucleic acids or for sequencing of unknown nucleic acids by a simple procedure using arrays uses nucleic acid analoges as sequence discriminators. This procedure simplifies the working mode in complex problematic cases.
摘要翻译：通过使用阵列的简单程序检测核酸中点突变和多态性或未知核酸测序的方法使用核酸类似物作为序列鉴定子。该过程简化了复杂问题案例中的工作模式。

138. 发明申请

US20020155586A1 Integrated portable biological detection system 失效
公开(公告)号：US20020155586A1
公开(公告)日：2002-10-24
申请号：US10163835
申请日：2002-06-05
申请人： Nanogen, Inc.
发明人： Jing Cheng , Lei Wu , Michael J. Heller , Edward L. Sheldon , Jonathan M. Diver , James P. O'Connell , Dan Smolko , Shila Jalali , David Willoughby
IPC分类号： C12M001/34
CPC分类号： G11C13/0019 , B01D57/02 , B01J19/0046 , B01J19/0093 , B01J2219/00317 , B01J2219/00585 , B01J2219/0059 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B01J2219/00853 , B01J2219/00914 , B01L3/5027 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0631 , B01L2200/0647 , B01L2200/0668 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/087 , B01L2300/0877 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0496 , B01L2400/0644 , B03C5/026 , B03C5/028 , B82Y5/00 , B82Y10/00 , C07H21/00 , C07K1/045 , C12N13/00 , C12Q1/6806 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N27/44743 , G11C13/0014 , G11C13/04 , G11C19/00 , H01L25/50 , H01L2924/0002 , C12Q2565/515 , C12Q2565/607 , C12Q2565/629 , C12Q2525/107 , C12Q2531/119 , C12Q2531/101 , C12Q2531/113 , H01L2924/00
摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a nulllaboratory-on-a-chipnull system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

139. 发明申请

US20020098117A1 Incubator for use in an automated diagnostic analyzer 有权
标题翻译：用于自动诊断分析仪的孵化器
公开(公告)号：US20020098117A1
公开(公告)日：2002-07-25
申请号：US10073346
申请日：2002-02-13
申请人： Gen-Probe Incorporated
发明人： Kelly G. Ammann , Robert E. Schneider , Robert J. Smith
IPC分类号： C12M001/36
CPC分类号： C12Q1/6834 , B01F9/0001 , B01F9/0014 , B01F9/0021 , B01F2009/0074 , B01L7/52 , B01L7/5255 , B01L9/06 , B01L2200/147 , B01L2300/1822 , B01L2300/1827 , B03C1/282 , B03C1/288 , B03C1/30 , B03C2201/26 , C12Q1/6813 , C12Q1/6832 , C12Q2537/10 , G01N35/0098 , G01N35/0099 , G01N35/025 , G01N35/1065 , G01N2035/00356 , G01N2035/00524 , G01N2035/0437 , G01N2035/0455 , G01N2035/103 , Y10T29/53657 , Y10T29/53683 , Y10T29/53843 , Y10T156/1105 , Y10T156/1906 , Y10T436/11 , Y10T436/111666 , Y10T436/113332 , Y10T436/114998 , Y10T436/119163 , Y10T436/2575
摘要： An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, preforming an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step.
摘要翻译：用于执行多个诊断测定的自动分析仪同时包括多个站或模块，其中对反应容器中包含的流体样品进行分析方面的分析。分析仪包括用于自动准备标本样品，在规定温度下规定时间孵育样品，预处理分析物分离程序以及确定目标分析物的存在的站。自动插座输送系统将反应容器从一个站移动到另一个站。分析仪还包括用于以机器可接近的方式携带多个样本管和一次性移液管尖端的装置，用于搅拌包含固体支持材料的悬浮液并用于提供用于机器通入的容器的靶捕获试剂的容器的装置，以及用于在温度受控的环境中容纳试剂容器的装置，并且提供用于机器通入的容器。执行自动诊断测定的方法包括用于分离和扩增靶分析物的自动化方法。该过程通过在站之间自动移动包含固体支持材料和流体样品的多个反应容器中的每一个来进行，用于孵育反应容器的内容物并将与固体支持物结合的目标分析物与流体样品分离。在分析物分离步骤之后和最终孵育步骤之前，将分离的分析物加入扩增试剂。

140. 发明授权

US06391546B1 Method for detecting target nucleotide sequence 有权
标题翻译：检测靶核苷酸序列的方法
公开(公告)号：US06391546B1
公开(公告)日：2002-05-21
申请号：US09147251
申请日：1998-11-12
申请人： Isao Karube , Shinya Sawata , Ryohei Nagata
发明人： Isao Karube , Shinya Sawata , Ryohei Nagata
IPC分类号： C12Q168
CPC分类号： C12Q1/6832 , C12Q1/686 , C12Q2531/107 , C12Q2565/501
摘要： An object of the present invention is to provide a method for detecting a target nucleotide sequence using a complementary nucleotide sequence that has an excellent sensitivity of detection. The method comprises the steps of converting the target nucleotide sequence to a partially double-stranded nucleotide sequence which is double-stranded at one part and single-stranded in the remaining part and detecting said partially double stranded nucleotide sequence using a nucleotide sequence that is complementary to the target nucleotide sequence.
摘要翻译：本发明的目的是提供使用检测灵敏度优异的互补核苷酸序列检测靶核苷酸序列的方法。该方法包括以下步骤：将目标核苷酸序列转化为部分双链核苷酸序列，其一部分为双链，其余部分为单链，并使用互补的核苷酸序列检测所述部分双链核苷酸序列到目标核苷酸序列。

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