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    • 137. 发明授权
    • Genetically modified clostridium thermocellum engineered to ferment xylose
    • 转基因热纤维素梭菌工程发酵木糖
    • US09546385B2
    • 2017-01-17
    • US13995402
    • 2011-12-22
    • Aaron ArgyrosTrisha BarrettNicky CaiazzaDave Hogsett
    • Aaron ArgyrosTrisha BarrettNicky CaiazzaDave Hogsett
    • C12P7/06C12N9/12C12N15/74C12N1/20C12N9/92C12P7/54C12P7/10
    • C12P7/06C12N1/20C12N9/1205C12N9/92C12N15/74C12P7/065C12P7/10C12P7/54C12Y207/01017C12Y503/01005Y02E50/16Y02E50/17Y02P20/52
    • One aspect of the invention relates to industrial bioconversion of the xylose portion of biomass materials into fuels and chemicals. Another aspect of the invention relates to industrial bioconversion of the xylan portion of biomass materials into fuels and chemicals. In one embodiment, the invention is directed to the bacterium Clostridium thermocellum, a highly cellulolytic organism that has much potential as a biocatalyst in a consolidated bioprocess configuration. In some embodiments, the invention is a genetic modification that confers the ability to ferment xylose to C. thermocellum and the strains created with this modification. In some embodiments, the genetic modification is composed of two genes contained in an operon from T. saccharolyticum. The genes express proteins with xylose isomerase (XI) and xylulokinase activites (XK). In other embodiments, the invention relates to a recombinant Clostridium thermocellum host cell capable of fermenting xylan.
    • 本发明的一个方面涉及生物质材料的木糖部分的工业生物转化为燃料和化学品。 本发明的另一方面涉及将生物质材料的木聚糖部分转化为燃料和化学品的工业生物转化。 在一个实施方案中,本发明涉及细菌嗜热纤维梭菌(Clostridium thermocellum),一种高度纤维素分解的生物体,其在整合的生物过程构型中作为生物催化剂具有很大的潜力。 在一些实施方案中,本发明是赋予将木糖发酵至热纤维梭菌的能力的遗传修饰以及用该修饰产生的菌株。 在一些实施方案中,遗传修饰由来自解糖松解糖酵母的操纵子中包含的两个基因组成。 该基因表达具有木糖异构酶(XI)和木酮糖激酶活性(XK)的蛋白质。 在其他实施方案中,本发明涉及能够发酵木聚糖的重组热纤梭菌宿主细胞。