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91. 发明申请

US20030124563A1 System for the internal qualitative and quantitative validation of marker indices 有权
公开(公告)号：US20030124563A1
公开(公告)日：2003-07-03
申请号：US10182013
申请日：2002-09-19
发明人： Johannes Gerdes
IPC分类号： C12Q001/68 , G01N033/53 , G01N033/567 , C12N005/06
CPC分类号： G01N33/57484 , Y10T436/101666 , Y10T436/105831 , Y10T436/108331 , Y10T436/25 , Y10T436/2525
摘要： The present invention relates to the qualitative and quantitative validation of marker indices, especially for medical diagnosis and particularly for the determination of the growth fraction in a sample with antibodies against the Ki-67 protein. Diagnostic kit for the quantification of a cell fraction labeled by a marker for in vitro diagnosis, characterized in that a pseudo-tissue is used for the intra- and inter-assay standardization of the marker index.

92. 发明授权

US06582574B1 pK-matched running buffers for gel electrophoresis 有权
标题翻译：用于凝胶电泳的pK匹配运行缓冲液
公开(公告)号：US06582574B1
公开(公告)日：2003-06-24
申请号：US09536392
申请日：2000-03-28
申请人： Qiang Liu , Steve S. Sommer
发明人： Qiang Liu , Steve S. Sommer
IPC分类号： B01D5702
CPC分类号： C07K1/26 , G01N27/44747 , Y10S435/967 , Y10T436/108331
摘要： pK-matched buffers, each containing two effective buffering components: one weak base and one weak acid which have similar pKa at 25° C. (within 0.3 pK units On agarose gels, the buffers in various concentrations were tested for separation of double-stranded DNA fragments with various DNA markers, agarose gel concentrations, and field strengths. Mobility was inversely proportional to the logarithm of molecular weight. The buffers provided high resolution without smearing at more dilute concentration than is possible with standard TAE (Tris/Acetate, pH 8.0) or TBE (Tris/Borate, pH 8.3) buffers. The buffers were also tested in 7M urea denaturing LongRanger™ sequencing gels and in non-denaturing polyacrylamide SSCP gels. The pK-matched buffers provide good separation and high resolution, at a broad range of potential pH values. In comparison to TAE and TBE, pK-matched buffers provide higher voltage and current stability, lower working concentration, more concentrated stock solutions, and lower current per unit voltage, resulting in less heat generation.
摘要翻译： pK匹配的缓冲液，每个含有两个有效的缓冲组分：一个弱碱和一个在25℃具有相似pKa的弱酸（在0.3 pK单位内）在琼脂糖凝胶上，测试各种浓度的缓冲液分离双链具有不同DNA标记的DNA片段，琼脂糖凝胶浓度和场强，迁移率与分子量的对数成反比。缓冲液提供高分辨率，不会在比标准TAE（Tris /醋酸盐，pH 8.0）下更浓的浓度）或TBE（Tris /硼酸盐，pH 8.3）缓冲液，还在7M尿素变性LongRanger TM测序凝胶和非变性聚丙烯酰胺SSCP凝胶中测试缓冲液，pK-匹配的缓冲液提供良好的分离和高分辨率与TAE和TBE相比，pK匹配缓冲液提供更高的电压和电流稳定性，更低的工作浓度，更集中的储备溶液，并降低每单位电压的电流，导致较少的发热量。

93. 发明申请

US20030114788A1 Dual-filled twin bag, a package and a method for forming a package for administering a solution 有权
标题翻译：双填充双袋，包装和用于形成用于施用溶液的包装的方法
公开(公告)号：US20030114788A1
公开(公告)日：2003-06-19
申请号：US10283654
申请日：2002-10-29
发明人： Michael R. Keilman , Walter A. Makaryk , Joseph A. Giertych
IPC分类号： A61M001/00
CPC分类号： A61M1/28 , A61J1/00 , A61M1/1668 , A61M1/1686 , Y10S128/24 , Y10S206/828 , Y10T436/108331
摘要： A dual-filled twin bag, a packaging including the twin bag, and a method for forming the packaged twin bags are provided. Each of two bags, a solution bag (10) and a drain bag (12) for a system used for a peritoneal dialysis procedure, are filled, at least partially, with a solution (14). A tubing set (16) connects the two filled bags (10,12) and may also be filled with solution (14) which is added to the bags (10,12) and the tubing set (16) during the manufacturing process. Alternatively, the tubing set (16) may be filled with air. The tubing set (16) is sandwiched between the solution bag (10) and the drain bag (12) and subsequently packaged in an overpouch (24). The method for forming the package including the bags filled with solution is simplified and the overall package size is reduced due to the sandwiched tubing set (16) between the solution bags (10,12) and the provision of solution in both bags (10,12).
摘要翻译：提供双填充双袋，包括双包的包装以及用于形成包装的双袋的方法。至少部分地用溶液（14）填充两个袋子中的每个袋，溶液袋（10）和用于腹膜透析程序的系统的排液袋（12）。管道组（16）连接两个填充的袋（10,12），并且还可以在制造过程中填充有添加到袋（10,12）和管组（16）中的溶液（14）。或者，管组（16）可以填充空气。管套（16）夹在溶液袋（10）和排放袋（12）之间，随后包装在一个外袋（24）中。用于形成包括填充有溶液的袋的包装的方法被简化，并且由于溶液袋（10,12）之间的夹套管组（16）和在两个袋（10,12）中提供溶液，整体包装尺寸减小， 12）。

94. 发明授权

US06573102B2 Lytic reagent composition for determination of nucleated blood cells 有权
标题翻译：用于测定有核血细胞的溶血剂试剂组合物
公开(公告)号：US06573102B2
公开(公告)日：2003-06-03
申请号：US09917530
申请日：2001-07-27
申请人： Yi Li , Jing Li
发明人： Yi Li , Jing Li
IPC分类号： G01N3100
CPC分类号： G01N33/80 , G01N15/12 , G01N15/1459 , G01N33/5002 , G01N33/721 , G01N2015/1006 , G01N2015/1493 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： A lytic reagent composition for measuring nucleated blood cells in a blood sample is described. The lytic reagent composition contains a quaternary ammonium surfactant, an ethoxylated phenol, and an ethoxylated alcohol. When mixed with a blood sample, the lytic reagent composition lyses red blood cells and enables a differentiation of nucleated red blood cells from other cell types by DC impedance measurement. The lytic reagent composition can further contain an organic ligand for determining total hemoglobin concentration of a blood sample photometrically. Further disclosed is a lytic reagent system including the lytic reagent composition and a diluent. In addition, a single reagent composition containing salts is also disclosed, which can be used without a separate diluent. The lytic reagent compositions can be used for concurrent measurement of nucleated red blood cells, WBC, and hemoglobin of a blood sample.
摘要翻译：描述了用于测量血液样品中的有核血细胞的溶解试剂组合物。溶解试剂组合物含有季铵表面活性剂，乙氧基化苯酚和乙氧基化醇。当与血液样品混合时，溶解试剂组合物溶解红细胞，并且通过DC阻抗测量使得有核红细胞与其它细胞类型分化。溶解试剂组合物还可以含有用于测定血样的总血红蛋白浓度的有机配体。还公开了包括溶解试剂组合物和稀释剂的裂解试剂系统。此外，还公开了含有盐的单一试剂组合物，其可在没有单独的稀释剂的情况下使用。裂解试剂组合物可用于同时测量血液样品的有核红细胞，WBC和血红蛋白。

95. 发明申请

US20030098272A1 Package assembly for piperazine-based membranes 有权
标题翻译：哌嗪基膜的封装组件
公开(公告)号：US20030098272A1
公开(公告)日：2003-05-29
申请号：US09972650
申请日：2001-10-05
发明人： Allyn R. Marsh , Russ Schaffenberg , Steven D. Jons , Roy A. Davis
IPC分类号： B01D063/10
CPC分类号： B01D67/0097 , B01D63/10 , B01D69/125 , B01D71/56 , C08G73/0633 , Y10T436/108331
摘要： A package assembly comprising a sealed water-impermeable bag enclosing: a spiral wound filtration element comprising a piperazine-based membrane, and an aqueous solution comprises a substantially non-oxidizable buffer having capacity to sequester at least 0.0025 moles per liter of hydrogen ions. The assembly preferably further includes a reducing agent. The assembly provides improved preservation for piperazine-based membranes and elements incorporating such membranes.
摘要翻译：一种封装组件，包括密封的不透水袋，其包围：包含哌嗪类膜的螺旋卷绕过滤元件和水溶液包含具有每升氢离子至少螯合至少0.0025摩尔的能力的基本上不可氧化的缓冲液。该组件优选还包括还原剂。该组合体为基于哌嗪的膜和结合这种膜的元件提供了改进的保存。

96. 发明授权

US06569682B2 Hematology control product with increased closed vial stability 有权
标题翻译：血液学控制产品具有增加的封闭瓶子稳定性
公开(公告)号：US06569682B2
公开(公告)日：2003-05-27
申请号：US09917452
申请日：2001-07-27
申请人： Michael Elliott , Dana B. Jacobs , Nery Ortiz , Wayne Goldson , Penny Whitley , Theodore J. Gerula
发明人： Michael Elliott , Dana B. Jacobs , Nery Ortiz , Wayne Goldson , Penny Whitley , Theodore J. Gerula
IPC分类号： G01N3100
CPC分类号： G01N33/96 , G01N2496/05 , Y10T436/10 , Y10T436/101666 , Y10T436/105831 , Y10T436/106664 , Y10T436/108331
摘要： An improved blood suspension media for hematological compositions, having particular utility with a red blood cell component for devices using electronic and optical means for blood determinations, and processes for using the suspension media. The suspension media finds particular utility in providing the hematology control product with a stable and consistent MCV and RDW for an extended product shelf life.
摘要翻译：用于血液学组合物的改进的血液悬浮培养基，其具有用于使用电子和光学手段用于血液测定的装置的红细胞组分的特别用途，以及使用悬浮介质的方法。悬浮介质特别适用于为血液控制产品提供稳定和一致的MCV和RDW以延长产品保质期。

97. 发明授权

US06521460B1 Method of carrying out blood tests 失效
标题翻译：进行血液检查的方法
公开(公告)号：US06521460B1
公开(公告)日：2003-02-18
申请号：US09180806
申请日：1999-02-08
申请人： Alexander W. M. Strasser , Karl Melber , Hans Dietrich Menssen , Eckard Thiel
发明人： Alexander W. M. Strasser , Karl Melber , Hans Dietrich Menssen , Eckard Thiel
IPC分类号： G01N3386
CPC分类号： G01N33/50 , G01N33/86 , G01N2333/815 , Y10T436/108331
摘要： The present invention relates to a method of carrying out blood tests. The invention is based on the finding that clinico-chemical blood parameters can be determined using not only blood serum but also blood plasma. Therefore, freshly taken blood samples, which are mixed with at least one thrombin inhibitor, can be used for determining both clinico-chemical parameters and hematological parameters. The clinico-chemical parameters can thus be determined using blood plasma, a separation of coagulable components being not required any more.
摘要翻译：本发明涉及进行血液检查的方法。本发明是基于不仅可以使用血清而且可以使用血浆来确定临床化学血液参数的发现。因此，与至少一种凝血酶抑制剂混合的新鲜采集的血液样品可用于确定临床化学参数和血液学参数。因此，可以使用血浆来确定临床化学参数，不再需要分离凝结组分。

98. 发明申请

US20020192818A1 Recovering cryopreserved conifer embryogenic cultures 有权
标题翻译：回收冷冻保存的针叶树胚胎发生培养物
公开(公告)号：US20020192818A1
公开(公告)日：2002-12-19
申请号：US09573160
申请日：2000-05-19
发明人： Michael Ryan Becwar , Sharon Anne Krueger
IPC分类号： C12N005/00 , C12N005/02
CPC分类号： C12N5/0025 , C12N5/04 , Y10T436/108331
摘要： This invention relates to a method for improving the growth and regeneration potential of embryogenic cell and tissue cultures of coniferous plants retrieved from cryopreservation. In particular, this invention relates to the use of abscisic acid in the post-cryopreservation recovery medium to improve both the growth and somatic embryo production of embryogenic cell and tissue cultures of conifers, thereby enabling more rapid proliferation of the embryogenic cultures and a subsequent increase in the yield of somatic embryos. This method is well-suited for employment with a number of biotechnological uses of embryogenic cultures of coniferous plants retrieved from cryopreservation, including use with embryogenic cultures of coniferous plants and with genetically transformed embryogenic cultures of coniferous plants for producing clonal planting stock useful for reforestation.
摘要翻译：本发明涉及一种提高从冷冻保存回收的针叶植物胚胎发生细胞和组织培养物的生长和再生潜力的方法。特别地，本发明涉及脱落酸在后冷冻保存回收培养基中的用途，以改善针叶树胚胎发生细胞和组织培养物的生长和体细胞胚胎产生，从而使胚胎发生培养物更快速增殖，并随后增加体细胞胚的产量。这种方法非常适合用于从冷冻保存中回收的针叶植物的胚胎发生培养物的许多生物技术用途，包括用于针叶植物的胚发生培养物和用于生产用于再造林的克隆种植原料的经遗传转化的针叶植物培养物。

99. 发明授权

US06482648B2 Stable troponin preparation and the use thereof as a calibrator/control in immunoassays 失效
标题翻译：稳定的肌钙蛋白制剂及其作为免疫测定中的校准物/对照物的用途
公开(公告)号：US06482648B2
公开(公告)日：2002-11-19
申请号：US08950240
申请日：1997-10-14
申请人： Margit Doth , Christoph Petry
发明人： Margit Doth , Christoph Petry
IPC分类号： G01N3353
CPC分类号： G01N33/6887 , G01N2333/4712 , Y10S435/965 , Y10S435/967 , Y10S435/972 , Y10S436/811 , Y10S436/815 , Y10S530/807 , Y10T436/10 , Y10T436/102499 , Y10T436/103332 , Y10T436/104165 , Y10T436/105831 , Y10T436/106664 , Y10T436/107497 , Y10T436/108331
摘要： The present invention relates to methods for the production of a stable troponin preparation and its use as a calibrator and/or control in immunoassays. The formulation is prepared from mammalian, preferably bovine, heart tissue which provides a calibrator/control composition which remains stable over a long period of time.
摘要翻译：本发明涉及生产稳定的肌钙蛋白制剂的方法及其在免疫测定中作为校准物和/或对照物的用途。制剂由哺乳动物，优选牛的心脏组织制备，其提供在长时间内保持稳定的校准物/对照组合物。

100. 发明申请

US20020160359A1 Stabilizing diluent for polypeptides and antigens 审中-公开
公开(公告)号：US20020160359A1
公开(公告)日：2002-10-31
申请号：US10123750
申请日：2002-04-15
发明人： Jeffrey W. Steaffens , Laura Panzarella
IPC分类号： C12Q001/70
CPC分类号： G01N33/53 , A61K39/00 , A61K47/02 , A61K47/10 , A61K47/183 , A61K47/186 , A61K47/26 , A61K47/42 , A61K2039/555 , Y10S424/81 , Y10S435/811 , Y10S436/826 , Y10S530/868 , Y10T436/10 , Y10T436/106664 , Y10T436/108331 , Y10T436/2525
摘要： Compositions for stabilizing polypeptides or antigens are described. These compositions are useful for stabilizing polypeptides or antigens stored in aqueous formulations. Such formulations can be used for various analytical or other methods.

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