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91. 发明申请

US20040018539A1 Oligonucleotides for use in determining the presence of human papilloma virus in a test sample 失效
标题翻译：用于确定测试样品中人乳头瘤病毒存在的寡核苷酸
公开(公告)号：US20040018539A1
公开(公告)日：2004-01-29
申请号：US10601913
申请日：2003-06-23
发明人： Patricia Gordon , Nick M. Carter , Steven T. Brentano , Philip W. Hammond
IPC分类号： C12Q001/68 , C07H021/04
CPC分类号： C12Q1/708 , C12Q1/6865 , C12Q1/6886 , C12Q2600/112 , C12Q2600/16
摘要： The present invention describes oligonucleotides targeted to HPV Type 16 and/or Type 18 nucleic acid sequences which are particularly useful to aid in detecting HPV type 16 and or 18. The oligonucleotides can aid in detecting HPV Type 16 and/or Type 18 in different ways such as by acting as hybridization assay probes, helper probes, and/or amplification primers.
摘要翻译：本发明描述了针对HPV16型和/或18型核酸序列的寡核苷酸，其特别有助于检测HPV 16型和/或18型。寡核苷酸可以以不同的方式帮助检测HPV 16型和/或18型例如通过作为杂交测定探针，辅助探针和/或扩增引物。

92. 发明授权

US06653069B2 Method for quality control of an attenuated vericella live vaccine 失效
公开(公告)号：US06653069B2
公开(公告)日：2003-11-25
申请号：US09913514
申请日：2001-08-15
申请人： Yasuyuki Gomi , Hiroki Sunamachi , Michiaki Takahashi , Koichi Yamanishi
发明人： Yasuyuki Gomi , Hiroki Sunamachi , Michiaki Takahashi , Koichi Yamanishi
IPC分类号： C12Q170
CPC分类号： C12N7/00 , A61K39/12 , A61K39/25 , A61K2039/5254 , C12N2710/16734 , C12N2710/24161 , C12Q1/705 , C12Q1/708
摘要： Disclosed is a method for quality control of an attenuated varicella live vaccine, which comprises subjecting the genomic DNA of a sample varicella vaccine virus to sequence analysis and confirming that the genomic DNA of the sample varicella vaccine virus conserves the 5,745th G, the 105,356th C, the 105,544th G, the 106,262nd C and the 107,252nd C without suffering mutation, wherein the nucleotide numbers are in accordance with the nucleotide numbering system of the nucleotide sequence of the genomic DNA of the varicella virus Dumas strain of SEQ ID NO: 1.

93. 发明申请

US20030175789A1 Method of detection of nucleic acids with a specific sequence composition 审中-公开
标题翻译：检测具有特定序列组成的核酸的方法
公开(公告)号：US20030175789A1
公开(公告)日：2003-09-18
申请号：US10407543
申请日：2003-04-03
发明人： Susan Weininger , Arthur M. Weininger
IPC分类号： C12Q001/68
CPC分类号： C12Q1/6811 , C12Q1/6804 , C12Q1/6813 , C12Q1/682 , C12Q1/6827 , C12Q1/6853 , C12Q1/703 , C12Q1/708 , C12Q2537/161 , C12Q2537/149 , C12Q2537/143 , C12Q2525/301
摘要： This invention is a novel method for detecting and localizing specific nucleic acid sequences in a sample with a high degree of sensitivity and specificity. The method and novel compositions used in the method involve the use of Probe Nucleic Acids, the production of nucleic acid binding regions and the use of nucleic acid Target Binding Assemblies to detect and localize specific Target Nucleic Acids. The detection and localization of the Target Nucleic Acid is accomplished even in the presence of nucleic acids which have similar sequences. The method provides for a high degree of amplification of the signal produced by each specific binding event. In particular, methods and compositions are presented for the detection of HIV and HPV nucleic acid in samples. These methods and compositions find use in diagnosis of disease, genetic monitoring, forensics, and analysis of nucleic acid mixtures. Some of the novel compositions used in the detection method are useful in preventing or treating pathogenic conditions.
摘要翻译：本发明是用于以高灵敏度和特异性检测和定位样品中特异性核酸序列的新方法。该方法中使用的方法和新颖的组合涉及使用探针核酸，核酸结合区的产生以及使用核酸靶结合组件来检测和定位特异性靶核酸。靶核酸的检测和定位即使在具有相似序列的核酸的存在下也能实现。该方法提供了通过每个特定结合事件产生的信号的高度放大。特别地，提出了用于在样品中检测HIV和HPV核酸的方法和组合物。这些方法和组合物可用于诊断疾病，遗传监测，取证和核酸混合物的分析。用于检测方法的一些新型组合物可用于预防或治疗病原性病症。

94. 发明授权

US06610473B1 Peptides for inhibiting HPV E6-proteins 失效
标题翻译：用于抑制HPV E6蛋白的肽
公开(公告)号：US06610473B1
公开(公告)日：2003-08-26
申请号：US09889136
申请日：2001-10-04
申请人： Karin Butz , Felix Hoppe-Seyler
发明人： Karin Butz , Felix Hoppe-Seyler
IPC分类号： C12Q170
CPC分类号： C07K14/005 , A61K38/00 , C07K14/00 , C12N2710/20022 , C12Q1/708 , G01N33/56983
摘要： The present invention relates to peptides suited for inhibiting HPV-E6 proteins, DNAs encoding them and the use of both, particularly for eliminating HPV tumor cells.
摘要翻译：本发明涉及适合于抑制HPV-E6蛋白的肽，编码它们的DNA和两者的用途，特别是用于消除HPV肿瘤细胞。

95. 发明申请

US20030055240A1 HPV specific oligonucleotides 审中-公开
标题翻译： HPV特异性寡核苷酸
公开(公告)号：US20030055240A1
公开(公告)日：2003-03-20
申请号：US10136427
申请日：2002-05-01
发明人： Peter C. Roberts , Bruce L. Frank , David E. Szymkowski , John S. Mills , John Goodchild , Jia L. Wolfe , Robert E. Kilkuskie , Isobel M. Greenfield , Veronica Sullivan
IPC分类号： C12Q001/70 , C07H021/04
CPC分类号： C12N15/1131 , A61K38/00 , C07H21/00 , C12N2310/3125 , C12N2310/314 , C12N2310/315 , C12N2310/321 , C12N2310/341 , C12N2310/345 , C12N2310/346 , C12Q1/708 , C12N2310/3521
摘要： The present invention discloses synthetic oligonucleotides complementary to a nucleic acid spanning the translational start site of human papillomavirus gene E1, and including at least 15 nucleotides. Also disclosed are methods and kits for inhibiting the replication of HPV, for inhibiting the expression of HPV nucleic acid and protein, for detection of HPV, and for treating HPV infections.
摘要翻译：本发明公开了与跨越人乳头状瘤病毒基因E1的翻译起始位点的核酸互补的包含至少15个核苷酸的合成寡核苷酸。还公开了用于抑制HPV的复制，用于抑制HPV核酸和蛋白质的表达，用于HPV检测和用于治疗HPV感染的方法和试剂盒。

96. 发明授权

US06511805B1 Methods for detecting papillomavirus DNA in blood plasma and serum 有权
公开(公告)号：US06511805B1
公开(公告)日：2003-01-28
申请号：US09653644
申请日：2000-08-31
申请人： Christopher D. Gocke , Neil Christensen
发明人： Christopher D. Gocke , Neil Christensen
IPC分类号： C12Q168
CPC分类号： C07K14/47 , C07K14/4746 , C07K14/82 , C07K2319/00 , C12Q1/683 , C12Q1/686 , C12Q1/6865 , C12Q1/6883 , C12Q1/6886 , C12Q1/708 , C12Q2600/112 , C12Q2600/156 , C12Q2600/16 , C12Q2535/125
摘要： This invention relates to the detection of extracellular papillomavirus DNA in blood plasma or serum from a human or animal. In particular, the invention relates to the detection, identification, evaluation, or monitoring of neoplastic, premalignant or malignant disease associated with a papillomavirus. The invention thereby provides methods for the identification of individuals at risk for, or having, cervical dysplasia, cervical intraepithelial neoplasia, or cervical cancer.

97. 发明授权

US06503704B1 Method, reagent and kit for genotyping human papilloma virus 有权
标题翻译：用于基因分型人乳头状瘤病毒的方法，试剂和试剂盒
公开(公告)号：US06503704B1
公开(公告)日：2003-01-07
申请号：US09673531
申请日：2001-06-25
申请人： James Mahony , Alan Seadler , Timothy Kierstead , Sylvia Chong
发明人： James Mahony , Alan Seadler , Timothy Kierstead , Sylvia Chong
IPC分类号： C12Q170
CPC分类号： C12Q1/708
摘要： The present invention is directed to a method of determining the genotype of a human papilomavirus in a sample by amplifying a portion of the L1 open reading frame of human papilomavirus genome with the amplification primer having SEQ ID NO:2, the sequencing primer having SEQ ID NO:3 and an additional sequencing primer specific to HPV51 having SEQ ID NO:5.
摘要翻译：本发明涉及通过用具有SEQ ID NO：2的扩增引物扩增人papilomavirus基因组的L1开放阅读框的一部分，测序引物具有SEQ ID NO：2来确定样品中人papilomavirus的基因型的方法 NO 3和具有SEQ ID NO：5的HPV51特异性的另外的测序引物。

98. 发明授权

US06495361B1 Method of producing infectious papillomavirus in placental cells 失效
标题翻译：在胎盘细胞中产生感染性乳头状瘤病毒的方法
公开(公告)号：US06495361B1
公开(公告)日：2002-12-17
申请号：US09621579
申请日：2000-07-21
申请人： Paul L. Hermonat , Yong Liu
发明人： Paul L. Hermonat , Yong Liu
IPC分类号： C12N700
CPC分类号： C12Q1/708 , A61K39/00 , C12N7/00 , C12N2710/20051 , G01N2333/025
摘要： The present invention is discloses an improved method of producing infectious papillomavirus in vitro, with a method comprising (a) introducing papillomavirus or papillomavirus DNA or portions thereof necessary for replication, into an epithelial cell; and (b) providing conditions that produce papillomavirus, wherein the conditions comprise not contacting the epithelial cell a fibroblast and does not comprise an organotypic raft culture or a dermal equivalent. The present invention also discloses a papillomavirus infected non-keratinocyte epithelial cell produced by the methods of the present invention. Further, uses of the disclosed method includes detection methods, methods for screening anti-papillomavirus drugs, methods of making recombinant papillomavirus for vaccines and studying the life cycle. Additionally, a method of reducing and assessing the risk of spontaneous abortion is disclosed.
摘要翻译：本发明公开了一种在体外产生感染性乳头瘤病毒的改进方法，其方法包括（a）将乳头状瘤病毒或乳头瘤病毒DNA或其复制所必需的部分引入上皮细胞; 和（b）提供产生乳头瘤病毒的条件，其中所述条件不包括使上皮细胞与成纤维细胞接触，并且不包括器官型筏培养物或皮肤等效物。本发明还公开了通过本发明的方法生产的乳头瘤病毒感染的非角化细胞上皮细胞。此外，所公开的方法的用途包括检测方法，用于筛选抗乳头状瘤病毒药物的方法，制备用于疫苗的重组乳头瘤病毒的方法和研究生命周期。另外，公开了减少和评估自然流产风险的方法。

99. 发明授权

US06482588B1 Detection and identification of human papillomavirus by PCR and type-specific reverse hybridization 有权
标题翻译：通过PCR和类型特异性反向杂交检测和鉴定人乳头瘤病毒
公开(公告)号：US06482588B1
公开(公告)日：2002-11-19
申请号：US09527030
申请日：2000-03-16
申请人： Leen-Jan Van Doorn , Wim Quint , Berhnard Kleter , Jan TerSchegget
发明人： Leen-Jan Van Doorn , Wim Quint , Berhnard Kleter , Jan TerSchegget
IPC分类号： C12Q170
CPC分类号： C12Q1/708 , C12Q2531/113
摘要： The present invention relates to a method for detection and/or identification of HPV present on a biological sample, comprising the steps of amplification of HPV polynucleic acids and of hybridization of said amplified ploynucleic acids to a number of probes. By means of PCR, a short fragment of the L1 gene of HPV is amplified. The amplimers are then contacted with probes that specifically hybridize to said short fragment of the L1 gene of either one or more than one HPV type. A preferred format is the reverse hybridization technique, more particularly the LiPA technique. The invention also relates to primers and probes to be used in a method of detection and/or identification of HPV and to a diagnostic kit to perform said method.
摘要翻译：本发明涉及一种检测和/或鉴定存在于生物样品上的HPV的方法，其包括扩增HPV多核酸和将所述扩增的核酸与多个探针杂交的步骤。通过PCR，扩增HPV的L1基因的短片段。然后将扩增产物与与一种或多于一种HPV类型的L1基因的所述短片段特异性杂交的探针接触。优选的形式是反向杂交技术，更具体地说是LiPA技术。本发明还涉及用于检测和/或鉴定HPV的方法的引物和探针以及用于执行所述方法的诊断试剂盒。

100. 发明申请

US20020155427A1 Detection and typing of human papillomavirus using PNA probes 审中-公开
标题翻译：使用PNA探针检测和分型人乳头瘤病毒
公开(公告)号：US20020155427A1
公开(公告)日：2002-10-24
申请号：US09825482
申请日：2001-04-03
发明人： Menashi A. Cohenford , Brian Lentrichia
IPC分类号： C12Q001/70 , C12Q001/68 , C07H021/04
CPC分类号： C12Q1/6888 , C12Q1/708
摘要： The invention provides methods for detection and typing of HPV infection using PNA probes. More specifically, methods are provided for detecting high-risk types of HPV infection with minimal numbers of PNA probes or using PNA probes to selectively amplify only high-risk types of HPV.
摘要翻译：本发明提供使用PNA探针检测和分型HPV感染的方法。更具体地，提供了用于用最少量的PNA探针检测高风险类型的HPV感染或使用PNA探针选择性扩增仅高风险类型的HPV的方法。

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