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91. 发明申请

US20080131883A1 Homogeneous Analyte Detection 失效
标题翻译：均匀分析物检测
公开(公告)号：US20080131883A1
公开(公告)日：2008-06-05
申请号：US11718379
申请日：2005-11-03
申请人： Thomas Adams , Edward Jablonski
发明人： Thomas Adams , Edward Jablonski
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： G01N33/542 , C07K16/3069 , C12N15/1075 , C12Q1/6804 , C12Q1/6832 , G01N2458/10 , C12Q2563/179 , C12Q2563/131 , C12Q2537/101 , C12Q2527/101
摘要： The present invention provides novel binding pair compositions of defined and limited stability comprising nucleic acid detection markers useful for the homogeneous, sensitive detection of analytes. Also provided are methods for the sensitive homogeneous detection of analytes, particularly analytes of clinical relevance. Kits for preparing binding pairs of the invention and for performing the methods of the invention are also provided.
摘要翻译：本发明提供了限定和有限稳定性的新型结合对组合物，其包含用于分析物的均相敏感检测的核酸检测标记。还提供了敏感均匀检测分析物，特别是临床相关性分析物的方法。还提供了用于制备本发明的结合对和用于实施本发明的方法的试剂盒。

92. 发明授权

US07374945B2 Method for enhancing the association rates of polynucleotides 有权
标题翻译：增强多核苷酸结合速率的方法
公开(公告)号：US07374945B2
公开(公告)日：2008-05-20
申请号：US10020596
申请日：2001-12-07
申请人： Michael M. Becker
发明人： Michael M. Becker
IPC分类号： G01N33/00 , C12Q1/68 , C12M1/36 , C07H21/04
CPC分类号： C12Q1/6818 , C12Q1/6832 , C12Q1/6839 , C12Q1/70 , C12Q2527/125
摘要： The present invention features a method and associated kit for increasing the association rate between polynucleotides having complementary, single-stranded regions, where the method includes providing to a test sample containing the complementary polynucleotides one or more synthetic, water soluble polycationic polymers.
摘要翻译：本发明的特征在于一种用于增加具有互补的单链区域的多核苷酸之间的缔合速率的方法和相关试剂盒，其中所述方法包括向含有互补多核苷酸的测试样品提供一种或多种合成的水溶性聚阳离子聚合物。

93. 发明申请

US20080076130A1 MOLECULAR HAPLOTYPING OF GENOMIC DNA 审中-公开
标题翻译：基因组DNA的分子生物学
公开(公告)号：US20080076130A1
公开(公告)日：2008-03-27
申请号：US11780351
申请日：2007-07-19
申请人： Baochuan Guo
发明人： Baochuan Guo
IPC分类号： C12Q1/68 , G01N33/48
CPC分类号： C12Q1/6876 , C12Q1/6832 , C12Q2600/156 , C12Q2600/172 , Y10T436/143333
摘要： A method of determining the haplotype structures of a nucleic acid comprising two or more single nucleotide polymorphisms (SNPs) of interest is provided. The method comprises obtaining an enriched nucleic acid fraction which comprises from 2 to 30 times more of one allelic variant of the nucleic acid than the other allelic variant of the nucleic acid, and genotyping the enriched nucleic acid fraction to identify the alleles in the two or more SNPs of interest that are present at a higher level or lower level than the other alleles in said two or more SNPs of interest, wherein the alleles that are present at a higher level are on the enriched allelic variant and form one haplotype and the alleles that are present at a lower level are on the non-enriched allelic variant and form the other haplotype. Kits for conducting the present methods are also provided.
摘要翻译：提供了确定包含感兴趣的两个或更多个单核苷酸多态性（SNP）的核酸的单元型结构的方法。该方法包括获得富集的核酸级分，其包含核酸的一种等位基因变体比核酸的其他等位基因变体的2至30倍，并且对富集的核酸级分进行基因分型以鉴定两者中的等位基因存在于所述两个或更多个目的SNP中的其他等位基因的较高水平或更低水平的更多SNP，其中存在于较高水平的等位基因位于富集的等位基因变体上并形成一个单倍型和等位基因存在于较低水平的是在非富集的等位基因变体上并形成另一个单倍型。还提供了用于执行本方法的套件。

94. 发明授权

US07348148B2 Nucleic acid derivatives 有权
标题翻译：核酸衍生物
公开(公告)号：US07348148B2
公开(公告)日：2008-03-25
申请号：US11365928
申请日：2006-03-02
申请人： David Segev
发明人： David Segev
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02
CPC分类号： C12Q1/6839 , C07B2200/11 , C12Q1/6832 , C12Q2525/117 , C12Q2525/113
摘要： A compound which comprises a backbone having a plurality of chiral carbon atoms, the backbone bearing a plurality of ligands each being individually bound to a chiral carbon atom of the plurality of chiral carbon atoms, the ligands including one or more pair(s) of adjacent ligands each containing a moiety selected from the group consisting of a naturally occurring nucleobase and a nucleobase binding group, wherein moieties of the one or more pair(s) are directly linked to one another via a linker chain; building blocks for synthesizing the compound; and uses of the compound, particularly in antisense therapy.
摘要翻译：包含具有多个手性碳原子的骨架的化合物，所述骨架具有多个配体，每个配体各自与多个手性碳原子的手性碳原子结合，所述配体包括一个或多个相邻的一对各自含有选自天然存在的核碱基和核碱基结合基团的部分的配体，其中所述一个或多个对的部分通过连接体链彼此直接连接; 用于合成化合物的结构单元; 和化合物的用途，特别是在反义治疗中。

95. 发明申请

US20070099221A1 Method of analyzing nucleic acid 审中-公开
标题翻译：分析核酸的方法
公开(公告)号：US20070099221A1
公开(公告)日：2007-05-03
申请号：US11588561
申请日：2006-10-27
申请人： Tomonori Nagaoka
发明人： Tomonori Nagaoka
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6832 , C12Q2545/101
摘要： An object of the invention is to avoid any possibility of error caused by a nucleic acid used as a standard, a probe, or probe spot, etc. or enable correction thereof, to analyze the frequency of gene expressions with high reproducibility, and to analyze any change of primary structure in a sample nucleic acid. The invention provides a method of analyzing the primary structure of at least one target base sequence present in a sample nucleic acid by detecting any signal from a sample nucleic acid hybrid composed of a probe and the same nucleic acid, wherein any signal from a synthetic standard nucleic acid hybrid composed of the probe and a synthetic standard nucleic acid containing at least one target base sequence is detected and on the basis of the signal from the synthetic standard nucleic acid hybrid, the signal from the sample nucleic acid hybrid is corrected to thereby effect analysis of the primary structure of the sample nucleic acid.
摘要翻译：本发明的目的是避免由用作标准的核酸，探针或探针斑点等引起的错误的任何可能性或能够进行校正，以高再现性分析基因表达的频率，并分析样品核酸中一级结构的任何变化。本发明提供了一种通过检测由探针和相同核酸组成的样品核酸杂交的任何信号来分析样品核酸中存在的至少一种靶碱基序列的一级结构的方法，其中来自合成标准检测由探针构成的核酸杂交体和含有至少一个靶基序列的合成标准核酸，根据来自合成标准核酸杂交体的信号，对来自样品核酸杂交体的信号进行校正，从而实现分析样品核酸的一级结构。

96. 发明申请

US20070099196A1 Novel oligonucleotide compositions and probe sequences useful for detection and analysis of micrornas and their target mRNAs 审中-公开
标题翻译：新的寡核苷酸组合物和探针序列可用于检测和分析微棱镜及其靶mRNA
公开(公告)号：US20070099196A1
公开(公告)日：2007-05-03
申请号：US11324177
申请日：2005-12-29
申请人： Sakari Kauppinen , Ronald Plasterk , Erno Wienholds , Wigard Kloosterman
发明人： Sakari Kauppinen , Ronald Plasterk , Erno Wienholds , Wigard Kloosterman
IPC分类号： C12Q1/68 , G06F19/00 , C07H21/04
CPC分类号： C12N15/111 , C12N2310/141 , C12N2320/11 , C12Q1/6813 , C12Q1/6827 , C12Q1/6832 , C12Q1/6841 , C12Q1/6883 , C12Q2600/158 , C12Q2600/178 , G16B25/00 , G16B30/00 , C12Q2527/107 , C12Q2525/207 , C12Q2525/101
摘要： The invention relates relates to ribonucleic acids and oligonucleotide probes useful for detection and analysis of microRNAs and their target mRNAs, as well as small interfering RNAs (siRNAs).
摘要翻译：本发明涉及可用于检测和分析微小RNA及其靶mRNA以及小干扰RNA（siRNA）的核糖核酸和寡核苷酸探针。

97. 发明申请

US20070042389A1 Method and sequences for determinate nucleic acid hybridization 失效
公开(公告)号：US20070042389A1
公开(公告)日：2007-02-22
申请号：US11205583
申请日：2005-08-16
申请人： William Hillis
发明人： William Hillis
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6874 , C12Q2525/101 , C12Q2525/117
摘要： Provided are methods for using nucleic acid sequences having two or more degenerately pairing nucleotides, each degenerate nucleotide having a partially overlapping set of complementarity, to reduce the number of hybridizing nucleotide sequences or probes used in biochemical and molecular biological operations having sequence specific hybridization. The method may be employed for various hybridization procedures with sequence specific hybridization, including sequencing methods measuring hybridization directly, and tagging by hybridization methods in which the sequence is determined by analyzing the pattern of tags that hybridize thereto, and hybridization dependent amplification methods. The method involves hybridizing to the nucleic acid sequence of interest a first hybridizing nucleotide sequence and a second hybridizing nucleotide sequence, each comprising a sequence complementary, or complementary except at a position of interest or variable position, to a nucleic acid sequence of interest, and analyzing the whether some, all or none of the probes or tags hybridize.

98. 发明申请

US20070031885A1 Materials and methods for detection of nucleic acids 有权
标题翻译：用于检测核酸的材料和方法
公开(公告)号：US20070031885A1
公开(公告)日：2007-02-08
申请号：US11546631
申请日：2006-10-12
申请人： David Marshall , James Prudent , Christopher Sherrill , Gideon Shapiro , Jennifer Grenier , Craig Richmond , Simona Jurczyk , Jerod Ptacin
发明人： David Marshall , James Prudent , Christopher Sherrill , Gideon Shapiro , Jennifer Grenier , Craig Richmond , Simona Jurczyk , Jerod Ptacin
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6853 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6834 , C12Q1/6858 , C12Q2600/156 , C12Q2565/1015 , C12Q2525/101 , C12Q2525/161
摘要： Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.
摘要翻译：描述了使用非天然碱的测定法。在一个实施方案中，所述方法包括使怀疑含有靶核酸的样品与聚合酶和第一和第二引物接触; 如果存在于样品中，则使用第一和第二引物通过PCR扩增靶核酸以产生具有双链区域和包含非天然碱基的单链区域的扩增产物; 使样品与包含与单链区域的非天然碱基互补的标记物和非天然碱基的报告子接触; 将至少一部分报告物退火至扩增产物的单链区域; 并将标记的信号与样品中靶核酸的存在相关联。本发明还提供用于检测样品中靶核酸的相应试剂盒。

99. 发明申请

US20060275785A1 Species-specific primer sets and identification of species-specific DNA sequences using genome fragment enrichment 有权
公开(公告)号：US20060275785A1
公开(公告)日：2006-12-07
申请号：US11316888
申请日：2005-12-27
申请人： Orin Shanks , Jorge Domingo , James Graham , Jingrang Lu
发明人： Orin Shanks , Jorge Domingo , James Graham , Jingrang Lu
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6809 , C12Q1/6832 , C12Q1/689 , C12Q2563/131 , C12Q2537/161
摘要： Targeted sequencing of genetic regions that differ between two DNA preparations uses genomic fragment enrichment. This method can be used to study genetic variation among closely related species and microbial communities.

100. 发明授权

US07138383B2 Treating cancer using an oligonucleotide N3′->N5′ thiophosphoramidate 有权
标题翻译：使用寡核苷酸N 3' - > N 5'硫代磷酰胺酸治疗癌症
公开(公告)号：US07138383B2
公开(公告)日：2006-11-21
申请号：US10967755
申请日：2004-10-18
申请人： Sergei Gryaznov , Krisztina Pongracz , Tracy Matray
发明人： Sergei Gryaznov , Krisztina Pongracz , Tracy Matray
IPC分类号： A61K31/70 , C07H21/00 , C12Q1/68
CPC分类号： C07H21/00 , C07H1/06 , C12N15/111 , C12N15/113 , C12N15/1137 , C12N2310/314 , C12N2320/10 , C12N2320/50 , C12N2320/51 , C12N2330/30 , C12Q1/6813 , C12Q1/6832 , C12Y207/07049
摘要： Oligonucleotides with a novel sugar-phosphate backbone containing at least one internucleoside 3′-NHP(O)(S−)O-5′ linkage, and methods of synthesizing and using the inventive oligonucleotides are provided. The inventive thiophosphoramidate oligonucleotides were found to retain the high RNA binding affinity of the parent oligonucleotide N3′→P5′ phosphoramidates and to exhibit a much higher acid stability.
摘要翻译：提供了含有至少一个核苷间3'-NHP（O）（O-O）5-O'键的新型糖 - 磷酸骨架的寡核苷酸，以及合成和使用本发明的寡核苷酸的方法。发现本发明的硫代磷酰胺化物寡核苷酸保留了母体寡核苷酸N 3 - →P 5'氨基磷酸酯的高RNA结合亲和力并表现出高得多的酸稳定性。

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