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91. 发明申请

US20060068408A1 Method for determining DNA damage and multi-chamber plate 审中-公开
标题翻译：确定DNA损伤和多腔板的方法
公开(公告)号：US20060068408A1
公开(公告)日：2006-03-30
申请号：US11069752
申请日：2005-03-01
申请人： Irene Witte , Hartmut De Wall
发明人： Irene Witte , Hartmut De Wall
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12N15/101 , C12N15/102 , C12Q1/6816 , C12Q2565/601
摘要： In a method for determining DNA damage, wherein cells are treated with genotoxic agents and lysed and DNA fragments of the cells are separated according to their length by a physical separating method, cells are put into various chambers of a multi-chamber plate and a genotoxic chemicals are put into the various chambers of the multi-chamber plate. The cytotoxicity is then measured and the walls of the multi-chamber plate are then removed and the physical separation process is carried out together for all samples. The multi-chamber plate has removable walls and at least one coating in the bottom region. Samples for testing for DNA damage can be investigated especially quickly, effectively and inexpensively by the method using the coated multi-chamber plate.
摘要翻译：在确定DNA损伤的方法中，其中细胞用基因毒素处理并裂解，细胞的DNA片段根据其长度通过物理分离方法分离，将细胞置于多室板和基因毒性的各个室中化学品被放入多室板的各个室中。然后测量细胞毒性，然后去除多室板的壁，并且对于所有样品一起进行物理分离过程。多室板具有可移除的壁和底部区域中的至少一个涂层。通过使用涂覆的多室板的方法，可以特别快速，有效和廉价地研究用于DNA损伤测试的样品。

92. 发明申请

US20050245733A1 Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography 有权
标题翻译：使用碳水化合物非离子型去垢剂与二氧化硅色谱法在质粒制备物中还原内毒素的方法
公开(公告)号：US20050245733A1
公开(公告)日：2005-11-03
申请号：US11108317
申请日：2005-04-18
申请人： Kevin Ray , Carol Kreader , Fuqiang Chen , David Cutter
发明人： Kevin Ray , Carol Kreader , Fuqiang Chen , David Cutter
IPC分类号： C07H21/04 , C12N15/10 , C12N15/74
CPC分类号： C07H21/04 , C12N15/101
摘要： The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography.
摘要翻译：本发明提供使用碳水化合物非离子洗涤剂与二氧化硅色谱法还原质粒制备中的内毒素的方法。

93. 发明申请

US20050161331A1 Fractionation of macro-molecules using asymmetric pulsed field electrophoresis 审中-公开
公开(公告)号：US20050161331A1
公开(公告)日：2005-07-28
申请号：US11075682
申请日：2005-03-09
申请人： Lotien Huang , James Sturm , Robert Austin
发明人： Lotien Huang , James Sturm , Robert Austin
IPC分类号： C07H21/04 , B01L3/00 , C12N15/10 , G01N27/447 , G01N27/453
CPC分类号： C12N15/101 , Y10T436/10 , Y10T436/11 , Y10T436/25
摘要： A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.

94. 发明申请

US20050080251A1 Method of separation using aromatic thioether ligands 失效
标题翻译：使用芳香族硫醚配体的分离方法
公开(公告)号：US20050080251A1
公开(公告)日：2005-04-14
申请号：US10504884
申请日：2003-02-14
申请人： Raf Lemmens
发明人： Raf Lemmens
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12N15/101
摘要： The present invention is a method of separating nucleic acid molecules from contaminants, such as proteins, in a solution and isolating one or more desired nucleic acid molecules, which method comprises the following steps (a) providing an aqueous adsorption solution, which includes nucleic acids and a salt that forms lyotropic ions when dissolved; (b) passing said solution over a matrix to adsorb the nucleic acids onto the matrix, said matrix including an aromatic ring moiety and at least one thioether moiety; (c) passing an aqueous eluent over said matrix to desorb the nucleic acid molecules therefrom, which eluent includes a salt that forms lyotropic ions and a gradient of increasing ionic strength originating from an increasing concentration of a salt that forms less lyotropic ions when dissolved than the ones present in said aqueous adsorption solution; and (d) isolating a fraction comprising the desired nucleic acid molecules.
摘要翻译：本发明是一种在溶液中分离核酸分子与污染物如蛋白质并分离一种或多种所需核酸分子的方法，该方法包括以下步骤（a）提供含水的吸附溶液，其包括核酸和溶解时形成溶致离子的盐; （b）将所述溶液通过基质以将核酸吸附到基质上，所述基质包括芳族环部分和至少一个硫醚部分; （c）将水性洗脱液通过所述基质以从其中解吸核酸分子，该洗脱液包括形成溶致离子的盐和来自增溶离子强度的梯度，所述梯度随溶解时形成较少溶致离子的盐的浓度增加而增加存在于所述含水吸附溶液中的那些; 和（d）分离包含所需核酸分子的级分。

95. 发明申请

US20050042619A1 Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof 审中-公开
标题翻译：使用位点特异性切口内切核酸酶产生单链区域及其应用
公开(公告)号：US20050042619A1
公开(公告)日：2005-02-24
申请号：US10657298
申请日：2003-09-08
申请人： William Jack , Ira Schildkraut , Julie Menin
发明人： William Jack , Ira Schildkraut , Julie Menin
IPC分类号： C12N15/10 , C12N15/64 , C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12N15/10 , C12N15/101 , C12N15/102 , C12N15/64 , Y10T436/143333
摘要： The present invention relates to the use of site-specific nucleic acid nicking enzymes to create single-stranded regions in duplex nucleic acids. Such single-stranded regions can take the form of gaps interior to the duplex, or terminal single-stranded regions. Single-stranded termini can be crafted to allow linkage of various elements via base-pairing with elements containing a complementary single-stranded region. This joining is useful, for example, in an ordered, oriented assembly of DNA modules to create cloning or expression vectors. This joining is also useful in attaching detection probes and purifying DNA molecules containing the single-stranded region. Gaps are useful in similar applications, including attaching detection or purification probes.
摘要翻译：本发明涉及位点特异性核酸切口酶在双链体核酸中产生单链区域的用途。这样的单链区域可以采取在双链体或末端单链区域内部的间隙的形式。可以制备单链末端以允许通过碱基配对与含有互补单链区域的元件连接各种元件。该连接可用于例如DNA模块的有序导向装配以产生克隆或表达载体。该连接对于附着检测探针和纯化含有单链区域的DNA分子也是有用的。间隙在类似的应用中是有用的，包括附着检测或纯化探针。

96. 发明申请

US20040209258A1 Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene 失效
标题翻译：使用包括聚氧化烯的阴离子交换材料从生物混合物中除去有机分子的方法和装置
公开(公告)号：US20040209258A1
公开(公告)日：2004-10-21
申请号：US10417609
申请日：2003-04-17
申请人： 3M Innovative Properties Company
发明人： Ranjani V. Parthasarathy , Rusty A. Rasmussen
IPC分类号： C12Q001/68 , C07H021/04
CPC分类号： C12N15/101 , B01J41/05 , B01J41/07 , B01J47/016
摘要： Methods and devices for removing small negatively charged molecules from a biological sample mixture that uses an anion exchange material that has associated therewith a polyoxyalkylene.
摘要翻译：用于从使用具有与其相关联的阴离子交换材料的生物样品混合物中去除小的带负电荷的分子的方法和装置。

97. 发明授权

US06806362B2 pH dependent ion exchange matrix and method of use in the isolation of nucleic acids 有权
标题翻译： pH依赖性离子交换基质和用于分离核酸的方法
公开(公告)号：US06806362B2
公开(公告)日：2004-10-19
申请号：US09813077
申请日：2001-03-20
申请人： Craig E. Smith , Diana L. Holmes , Daniel J. Simpson , Jehoshua Katzenhendler , Rex M. Bitner , Josephine C. Grosch
发明人： Craig E. Smith , Diana L. Holmes , Daniel J. Simpson , Jehoshua Katzenhendler , Rex M. Bitner , Josephine C. Grosch
IPC分类号： C07G300
CPC分类号： C12N15/101 , B01J39/26 , B01J41/20
摘要： Disclosed are pH dependent ion exchange matrices, methods of making, and methods of use.
摘要翻译：公开了依赖于pH的离子交换基质，制备方法和使用方法。

98. 发明授权

US06783962B1 Particulate material for purification of bio-macromolecules 有权
标题翻译：用于净化生物大分子的颗粒物质
公开(公告)号：US06783962B1
公开(公告)日：2004-08-31
申请号：US09936925
申请日：2001-11-20
申请人： Morten Aae Olander , Allan Otto Fog Lihme , Timothy John Hobley , Marcos Simon , Irini Theodossiou , Owen Robert Tyrynis Thomas
发明人： Morten Aae Olander , Allan Otto Fog Lihme , Timothy John Hobley , Marcos Simon , Irini Theodossiou , Owen Robert Tyrynis Thomas
IPC分类号： C12P1934
CPC分类号： G01N33/54346 , C12N1/02 , C12N15/101 , C12P19/34 , G01N33/548 , Y10S435/803 , Y10S435/815 , Y10S530/811 , Y10S530/813 , Y10S530/815
摘要： The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 &mgr;m, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.
摘要翻译：本发明涉及密度为至少2.5g / ml的颗粒材料，其中颗粒材料的颗粒具有5-75μm的平均直径，并且颗粒材料的颗粒基本上由聚合物基质基质构成，例如多糖如琼脂糖，以及无孔核心材料，例如，所述核心材料具有至少3.0g / ml的密度，所述聚合物基质基质包括在pH4.0下带正电荷的侧基，或者是生物分子的亲和配体。可能的侧基包括聚乙烯亚胺（PEI），二乙基氨基乙基（DEAE）和季氨基乙基（QAE）。这些材料可用于扩展床或流化床色谱法，特别是用于纯化生物大分子如质粒DNA，染色体DNA，RNA，病毒DNA，细菌和病毒。

99. 发明申请

US20040157244A1 Process for purification of plasmid DNA 审中-公开
标题翻译：纯化质粒DNA的方法
公开(公告)号：US20040157244A1
公开(公告)日：2004-08-12
申请号：US10719978
申请日：2003-11-24
申请人： Vical Incorporated
发明人： Gregg Budahazi , Blake Goff
IPC分类号： C12Q001/68 , C07H021/04
CPC分类号： C12N15/1017 , C12N15/101
摘要： The invention relates to a process for purifying plasmid DNA. The invention also relates to a DNA product made by the process of the invention. The DNA product is suitable for pharmaceutical use.
摘要翻译：本发明涉及一种纯化质粒DNA的方法。本发明还涉及通过本发明的方法制备的DNA产物。该DNA产品适用于药物使用。

100. 发明申请

US20040127648A1 Sorbent and method for the separation of plasmid DNA 审中-公开
标题翻译：吸附剂和分离质粒DNA的方法
公开(公告)号：US20040127648A1
公开(公告)日：2004-07-01
申请号：US10334135
申请日：2002-12-31
申请人： Ciphergen Biosystems, Inc.
发明人： Luc Guerrier , Egisto Boschetti
IPC分类号： C08L033/06
CPC分类号： C12N15/101 , C08L33/14 , C08L33/24 , C08L51/003 , C08L53/00 , C12N15/1006 , C08L2666/04 , C08L2666/02
摘要： A polymer that is useful for separating plasmid DNA from cell lysates can be dispersed throughout the pores of a porous matrix, forming a chromatographic sorbent. Illustrative of such a polymer is one that comports with the formula: 1 where R1 is C1-6 alkyl; each R2 is independently selected from the group consisting of H and C1-6 alkyl, optionally substituted with 1 to 3 OH groups; R3 is selected from H, C1-14 alkyl, and (C1-14 alkyl)aryl; each n is independently an integer from 1 to 6; a is at least 1; b is at least 1; w is an integer from 0 to 10; and z is at least 1. X is an anion; each Y is independently selected from the group consisting of H, C1-6 alkyl, and a linear homopolymer comprised of monomers, each of them substituted with pendant amines, provided that at least one Y is the linear homopolymer; and null-----null represents the remainder of the polymer.
摘要翻译：可用于从细胞裂解物分离质粒DNA的聚合物可分散在多孔基质的整个孔中，形成色谱吸附剂。这样的聚合物的例子是与下式相符的聚合物：其中R 1是C 1-6烷基; 每个R 2独立地选自H和C 1-6烷基，任选被1至3个OH基团取代; R 3选自H，C 1-4烷基和（C 1-4烷基）芳基; 每个n独立地为1至6的整数; a至少为1; b至少为1; w为0〜10的整数; z为至少1. X为阴离子; 每个Y独立地选自H，C 1-6烷基和由单体组成的线性均聚物，它们各自被侧胺取代，条件是至少一个Y是线性均聚物; 和“-----”表示聚合物的其余部分。

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