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91. 发明申请

US20100083392A1 HAFNIA PHYTASE VARIANTS 有权
公开(公告)号：US20100083392A1
公开(公告)日：2010-04-01
申请号：US12568312
申请日：2009-09-28
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
IPC分类号： A01K67/00 , C12N9/16 , C12P1/00 , C12P7/06 , C07H21/00 , C12N15/63 , C12N1/00 , C12P21/00 , A01H5/00 , A23L1/28 , A23K1/18 , A23K1/16 , C12G1/00 , C12C11/00 , C12C7/00
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译：本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度简介和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

92. 发明授权

US07271139B2 Lipolytic enzyme genes 有权
标题翻译：脂解酶基因
公开(公告)号：US07271139B2
公开(公告)日：2007-09-18
申请号：US11474151
申请日：2006-06-23
申请人： Noriko Tsutsumi , Jesper Vind , Shamkant Anant Patkar
发明人： Noriko Tsutsumi , Jesper Vind , Shamkant Anant Patkar
IPC分类号： A21D2/00 , C12N9/20
CPC分类号： C11D3/386 , A21D8/042 , C11D3/38627 , C12N9/20 , C12N15/1027
摘要： The inventors have isolated novel lipolytic enzyme genes with a high homology to the T. lanuginosus lipase gene and thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).
摘要翻译：本发明人已经分离出与丝状芽孢杆菌脂肪酶基因具有高同源性的新型脂肪分解酶基因，因此非常适合用于基因改组。因此，本发明提供了一种通过编码脂肪分解酶的两个或更多个同源基因的家族洗牌来产生脂肪分解酶的遗传多样性的方法。 DNA混洗技术用于创建洗牌基因文库，并在合适的表达系统中表达，并筛选表达的蛋白质以进行脂肪分解酶活性。本发明还提供了包含编码脂肪分解酶和脂肪分解酶（具有脂肪分解酶活性的多肽）的核苷酸序列的多核苷酸。

93. 发明申请

US20070010418A1 Lipolytic enzyme genes 有权
公开(公告)号：US20070010418A1
公开(公告)日：2007-01-11
申请号：US11474151
申请日：2006-06-23
申请人： Noriko Tsutsumi , Jesper Vind , Shamkant Anant
发明人： Noriko Tsutsumi , Jesper Vind , Shamkant Anant
IPC分类号： C11D3/386 , C07H21/04 , C12P21/06 , C12N15/74 , C12N1/21 , C12N9/20
CPC分类号： C11D3/386 , A21D8/042 , C11D3/38627 , C12N9/20 , C12N15/1027
摘要： The inventors have isolated novel lipolytic enzyme genes with a high homology to the T. lanuginosus lipase gene and thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).

94. 发明授权

US06767701B1 Methods of constructing and screening a DNA library of interest in filamentous fungal cells 有权
标题翻译：在丝状真菌细胞中构建和筛选感兴趣的DNA文库的方法
公开(公告)号：US06767701B1
公开(公告)日：2004-07-27
申请号：US09426038
申请日：1999-10-25
申请人： Jesper Vind
发明人： Jesper Vind
IPC分类号： C07H2104
CPC分类号： C12N15/80 , C12N9/20 , C12N15/1034
摘要： A method of constructing and screening a library of polynucleotide sequences of interest in filamentous fungal cells by use of an episomal replicating AMA1-based plasmid vector, thus achieving a high frequency of transformation and a stable and standard uniformly high level of gene expression.
摘要翻译：通过使用附加型复制的基于AMA1的质粒载体构建和筛选丝状真菌细胞中的多核苷酸序列文库的方法，从而实现高频率的转化和稳定和标准均匀的高水平的基因表达。

95. 发明授权

US06645749B2 Lipolytic enzyme 失效
公开(公告)号：US06645749B2
公开(公告)日：2003-11-11
申请号：US09931401
申请日：2001-08-16
申请人： Jesper Vind
发明人： Jesper Vind
IPC分类号： C12N920
CPC分类号： C11D3/38627 , A21D8/042 , C12N9/20
摘要： The present invention is directed to phospholipase from Fusarium solani (MUCL38667) and analogues thereof, nucleic acid constructs, recombinant expression vectors, and recombinant host comprising the nucleic acid sequences encoding the phospholipase from Fusarium solani (MUCL38667) and analogues thereof, methods of making same, and method of using the phosopholipases of the present invention in baking methods, baking compositions and detergent compositions.

96. 发明授权

US06518042B1 Process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells 失效
标题翻译：使用参与丝状真菌细胞错配修复系统的新型克隆基因在丝状真菌细胞中制备DNA文库的方法
公开(公告)号：US06518042B1
公开(公告)日：2003-02-11
申请号：US09512250
申请日：2000-02-24
申请人： Torben Vedel Borchert , Lars Christiansen , Jesper Vind
发明人： Torben Vedel Borchert , Lars Christiansen , Jesper Vind
IPC分类号： C12P2102
CPC分类号： C07K14/38 , C12N15/80
摘要： A process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells.
摘要翻译：使用涉及丝状真菌细胞的错配修复系统的新的克隆基因在丝状真菌细胞中制备DNA文库的方法。

97. 发明授权

US6159687A Methods for generating recombined polynucleotides 失效
标题翻译：产生重组多核苷酸的方法
公开(公告)号：US6159687A
公开(公告)日：2000-12-12
申请号：US040697
申请日：1998-03-18
申请人： Jesper Vind
发明人： Jesper Vind
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1093 , C12N15/1027
摘要： A method for in vitro construction of a library of recombined homologous polynucleotides from a number of different starting DNA templates and primers by induced template shifts during an polynucleotide synthesis is described, wherebyA. extended primers are synthesized bya) denaturing the DNA templatesb) annealing primers to the templates,c) extending the said primers by use of a polymerase,d) stop the synthesis, ande) separate the extended primers from the templates,B. a template shift is induced bya) isolating the extended primers from the templates and repeating steps A.b) to A.e) using the extended primers as both primers and templates, orb) repeating steps A.b) to A.e),C. this process is terminated after an appropriate number of cycles of process steps A. and B.a), A. and B.b), or combinations thereof.Optionally the polynucleotides are amplified in a standard PCR reaction with specific primers to selectively amplify homologous polynucleotides of interest.
摘要翻译：描述了在多核苷酸合成期间通过诱导的模板位移从许多不同的起始DNA模板和引物体外构建重组的同源多核苷酸文库的方法，其中A.通过a）使DNA模板变性来合成扩展的引物b）将引物退火至模板，c）通过使用聚合酶扩增所述引物，d）停止合成，和e）将延伸的引物与模板分离。B.通过以下步骤诱导模板位移：a）模板和重复步骤Ab）至Ae），使用扩展引物作为引物和模板，或b）重复步骤Ab）至Ae），C.该过程在经过适当数量的工艺步骤A和B的循环之后终止），A.和Bb）或其组合。任选地，在与特异性引物的标准PCR反应中扩增多核苷酸以选择性扩增目的同源多核苷酸。

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