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91. 发明授权

US5057438A Electrophoretic antigen-antibody determination with laminate of multiple membranes 失效
标题翻译：电泳抗原 - 抗体用多层膜叠层测定
公开(公告)号：US5057438A
公开(公告)日：1991-10-15
申请号：US31665
申请日：1987-03-30
申请人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
发明人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
IPC分类号： G01N33/561
CPC分类号： G01N33/561 , Y10S436/809 , Y10S436/824
摘要： Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.
摘要翻译：通过包括在电泳载体上形成多个不同种类的抗体或抗原的不同种类的反应膜，叠加这些反应膜，任选地将过滤器叠加在一起的方法来实现多种抗体或抗原的测定反应膜的层压体，将层压体插入电解质中，加入与上述反应膜中支持的多种抗体或抗原相对应的多种不同种类的抗原或抗体，通过电解质电泳移动加入的抗原或抗体并使其能够与支持在反应膜上的抗体或抗原反应，并测量由反应产生的抗原或抗体的浓度，或反应膜上以未反应形式负载的抗体或抗原的浓度。

92. 发明授权

US4971950A Support sheet for thermal transfer image-receiving sheet and method of producing same 失效
标题翻译：热转印图像接收片用支撑片及其制造方法
公开(公告)号：US4971950A
公开(公告)日：1990-11-20
申请号：US367075
申请日：1989-06-16
申请人： Masaru Kato , Toshihiro Minato , Toshikazu Nagura , Susumu Hasegawa , Kenji Yasuda
发明人： Masaru Kato , Toshihiro Minato , Toshikazu Nagura , Susumu Hasegawa , Kenji Yasuda
IPC分类号： B41M5/26 , B41M5/382 , B41M5/41 , B41M5/42 , H02G5/06
CPC分类号： B41M5/42 , B41M5/38214 , B41M5/41 , Y10S428/91 , Y10S428/913 , Y10S428/914 , Y10T428/31913 , Y10T428/31938
摘要： A support sheet useful for a thermal transfer image receiving sheet having a high resistance to thermal curling and capable of recording clear and uniform images or pictures thereon, comprises a sheet substrate with a thickness of 20 to 300 .mu.m, and multilayer front and back surface coating plastic films each comprising a pigment and a polyolefin resin, each having at least one bi-axially oriented base layer, and bonded to the sheet substrate, and satisfies the following relationships:S.sub.1 /S.sub.2 .ltoreq.1/2S.sub.3

93. 发明授权

US4824778A Immunoassay for measuring the concentration of antigen in a sample 失效
标题翻译：用于测量样品中抗原浓度的免疫测定
公开(公告)号：US4824778A
公开(公告)日：1989-04-25
申请号：US867554
申请日：1986-05-28
申请人： Keiichi Nagai , Daizo Tokinaga , Kazumichi Imai , Kenji Yasuda , Satoshi Takahashi , Teruaki Kobayashi
发明人： Keiichi Nagai , Daizo Tokinaga , Kazumichi Imai , Kenji Yasuda , Satoshi Takahashi , Teruaki Kobayashi
IPC分类号： G01N33/543 , G01N33/561 , G01N33/53
CPC分类号： G01N33/561 , Y10S435/968
摘要： An immunoassay comprisingimmobilizing antibody in a matrix for electrophoresis;immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis;either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; andmeasuring the concentration of antigen in the sample, characterized byusing as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance,moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis,reacting the substrate with the label enzyme to convert the same into a fluorescent substance, andmeasuring the concentration of the fluorescent substance in the electrolyte solution.
摘要翻译：免疫测定法，其包括将抗体固定在用于电泳的基质中; 通过使抗原通过电泳移动的方法，将抗原固定在测量样品中，使其与上述固定化抗体进行抗原抗体反应; 通过电泳将标记抗体移动到上述固定化抗原上，使其与固定化抗原反应，或通过电泳将标记的抗原移动到上述固定化抗体的未反应部分，使其与未反应部分反应; 测定样品中的抗原浓度，其特征在于，将标记抗体或标记抗原的标记作为能够将底物覆盖成荧光物质的酶，通过电泳将所述酶转化为荧光物质，使底物与标记酶反应，将其转化为荧光物质，并测量电解质溶液中荧光物质的浓度。

94. 发明授权

US4541717A Attraction holding device 失效
标题翻译：吸引力装置
公开(公告)号：US4541717A
公开(公告)日：1985-09-17
申请号：US559366
申请日：1983-12-08
申请人： Shigeru Itamoto , Kenji Yasuda , Senji Mikami
发明人： Shigeru Itamoto , Kenji Yasuda , Senji Mikami
IPC分类号： B25B11/00 , G01B5/00 , G01B9/02
CPC分类号： B25B11/005 , G01B5/0007 , G01B9/02
摘要： A device for holding a measurement object e.g. in interferometers comprises a base plate and an attraction plate mounted on the base plate so as to attract the object placed on an upper surface of the attraction plate to the base plate together with the attraction plate by a vacuum applied via attraction grooves formed on the base plate and attraction passages perforated through the attraction plate. The upper surface of the base plate is formed as a reflection plane, and the attraction plate is made of a transparent material, so that Newton's rings generated by light reflected from the upper surface of the base plate and light reflected from the lower surface of the attraction plate can be observed from above the attraction plate.
摘要翻译：一种用于保持测量对象的设备，例如在干涉仪中包括基板和安装在基板上的吸引板，以便通过在底座上形成的吸引槽将吸引板的上表面的物体与吸引板一起吸引到基板上板和吸引通道穿过吸引板。基板的上表面形成为反射面，吸引板由透明材料制成，从而从基板的上表面反射的光产生的牛顿环和从底板的下表面反射的光吸引板上方可观察到吸引板。

95. 发明授权

US08642306B2 Flow cytometer having cell-sorting function and method of separating living cells 失效
标题翻译：具有细胞分选功能的流式细胞仪和分离活细胞的方法
公开(公告)号：US08642306B2
公开(公告)日：2014-02-04
申请号：US12663373
申请日：2008-06-11
申请人： Shigeyuki Nakada , Noriaki Kimura , Akihide Ito , Kenji Yasuda
发明人： Shigeyuki Nakada , Noriaki Kimura , Akihide Ito , Kenji Yasuda
IPC分类号： C12N13/00
CPC分类号： G01N15/147 , C12M47/04 , G01N2015/149
摘要： A liquid sample flow containing living cells is irradiated with measurement laser light and the photo data of at least either scattering light or fluorescence that is generated by each of the living cells in the liquid sample flow due to the irradiation with the measurement laser light is acquired. Based on the photo data thus acquired, it is determined whether each of the cells assignable to the respective photo data is an unnecessary living cell or a target living cell. Based on the determination results, a pulse voltage is then applied exclusively to the living cells having been determined as unnecessary living cells so that the unnecessary living cells are damaged and killed.
摘要翻译：用测量激光照射含有活细胞的液体样品流，并且获取由于测量激光的照射而在液体样品流中的每个活细胞产生的至少一种散射光或荧光的照片数据。基于这样获得的照片数据，确定分配给各个照片数据的每个细胞是否是不必要的活细胞或目标活细胞。基于测定结果，脉冲电压被专门用于被确定为不必要的活细胞的活细胞，使得不必要的活细胞被损坏和死亡。

96. 发明申请

US20130314526A1 CELL ANALYSIS DEVICE 审中-公开
标题翻译：细胞分析装置
公开(公告)号：US20130314526A1
公开(公告)日：2013-11-28
申请号：US13882257
申请日：2011-09-16
申请人： Kenji Yasuda , Hideyuki Terazono , Hyonchol Kim , Masahito Hayashi , Akihiro Hattori , Kazuto Nishio , Tokuzo Arao , Yohei Miyagi , Takashi Ohtsu
发明人： Kenji Yasuda , Hideyuki Terazono , Hyonchol Kim , Masahito Hayashi , Akihiro Hattori , Kazuto Nishio , Tokuzo Arao , Yohei Miyagi , Takashi Ohtsu
IPC分类号： G06T7/00
CPC分类号： G06T7/0016 , G01N15/1475 , G01N15/1484 , G01N27/447 , G01N33/487 , G01N2015/1006 , G01N2015/1472 , G01N2015/149 , G01N2015/1493 , G01N2015/1497
摘要： Provided is a cell concentration/purification device having a function of successively locating cells in a specific area of a microchannel, and a function of sequentially capturing cell images by use of light from a plurality of monochromatic light sources on an image basis, and performing comparative analysis of the cell images to recognize individual cells based on information on the shape of the cells and an absorption spectral distribution of the cells or inside of the cells, thereby selectively separating/purifying the cells.
摘要翻译：本发明提供一种细胞浓度/净化装置，其具有将细胞连续定位在微通道的特定区域中的功能，以及通过使用来自多个单色光源的光以图像为基础顺序捕获细胞图像的功能，并进行比较基于关于细胞形状的信息和细胞的吸收光谱分布或细胞内部的信息来分析细胞图像以识别个体细胞，从而选择性地分离/纯化细胞。

97. 发明申请

US20130230881A1 METHOD AND APPARATUS FOR TESTING CARDIOTOXICITY AND EVALUATING CARDIOMYOCYTES 有权
标题翻译：用于测试心脏病毒和评估心脏病的方法和装置
公开(公告)号：US20130230881A1
公开(公告)日：2013-09-05
申请号：US13876992
申请日：2011-09-30
申请人： Kenji Yasuda , Tomoyuki Kaneko , Fumimasa Nomura
发明人： Kenji Yasuda , Tomoyuki Kaneko , Fumimasa Nomura
IPC分类号： C12Q1/02
CPC分类号： C12Q1/025 , C12M35/02 , C12M41/46 , C12M41/48 , G01N33/48728 , G01N33/5061 , G01N33/5088 , G01N2800/32
摘要： In the present invention, a cardiomyocyte cluster is disposed on a transparent substrate, and the quality of the cardiomyocytes is evaluated from the response of the cells to a forced pulsation stimulus applied to the cardiomyocytes. The cardiomyocyte cluster is disposed on the transparent substrate, and is exposed to the flow of a liquid containing an agent in a manner so that the agent acts on the cells, which configure a network. The extent of cardiac toxicity resulting from the agent is evaluated from measuring the fluctuations obtained from a comparison of adjacent cardiomyocytes of the network.
摘要翻译：在本发明中，将心肌细胞簇设置在透明基质上，根据细胞对施加于心肌细胞的强制脉动刺激的反应来评价心肌细胞的质量。将心肌细胞簇设置在透明基板上，并且以使得试剂作用于构成网络的细胞的方式暴露于含有试剂的液体的流动。通过测量从网络的相邻心肌细胞的比较获得的波动来评估由该试剂产生的心脏毒性的程度。

98. 发明申请

US20130029407A1 CELL ANALYZER 有权
标题翻译：细胞分析仪
公开(公告)号：US20130029407A1
公开(公告)日：2013-01-31
申请号：US13261410
申请日：2011-02-24
申请人： Hideyuki Terazono , Kenji Yasuda , Masahito Hayashi , Hiroyuki Takei , Akihiro Hattori
发明人： Hideyuki Terazono , Kenji Yasuda , Masahito Hayashi , Hiroyuki Takei , Akihiro Hattori
IPC分类号： C12M1/42
CPC分类号： G01N1/31 , C12N1/066 , G01N1/34 , G01N1/4077 , G01N15/1459 , G01N15/1463 , G01N21/6408 , G01N21/6428 , G01N2001/4094 , G01N2015/149 , G01N2021/6482 , Y10T436/11
摘要： Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof.
摘要翻译：提供一种细胞浓缩和净化装置，其具有：连续浓缩细胞的功能; 随后随后将细胞连续地放置在通道的特定区域中的功能; 基于图像同时识别每个单个细胞的形状和荧光发射的功能; 以及基于与其形状和荧光发射有关的数据识别细胞，然后分离和纯化细胞的功能。

99. 发明授权

US08202720B2 Model cell chip, apparatus for evaluating drug effect using the model cell chip and method of evaluating drug effect 有权
标题翻译：模型细胞芯片，使用模型细胞芯片评估药物效应的装置和药物效应评价方法
公开(公告)号：US08202720B2
公开(公告)日：2012-06-19
申请号：US12663468
申请日：2008-06-06
申请人： Kenji Yasuda , Atsushi Sugiyama , Kentaro Ando , Fumimasa Nomura , Hideyuki Terazono , Tomoyuki Kaneko , Mamoru Fukushima
发明人： Kenji Yasuda , Atsushi Sugiyama , Kentaro Ando , Fumimasa Nomura , Hideyuki Terazono , Tomoyuki Kaneko , Mamoru Fukushima
IPC分类号： C12M1/34 , C12M3/00 , C12N5/071 , A61K35/34
CPC分类号： G01N33/5061 , C12N2503/02 , G01N33/4836 , G01N33/54366
摘要： The present invention provides an apparatus for evaluating a drug effect enabling on-chip evaluation of the effect of a drug while the drug is acting on hERG-expressing cells. The present invention also provides a myocardial toxicity test apparatus and method therefor enabling in vitro myocardial toxicity testing that has previously been performed in vivo. A pulsating cell population and hERG-expressing cells (target model cells) are suitably isolated and arranged on a transparent substrate so that the two form gap junctions. The hERG-expressing cells are arranged on transparent electrodes provided on the transparent substrate. The hERG-expressing cells are exposed to a flow of a liquid containing a drug such that the drug acts thereon. The difference between the normal pulsation of hERG-expressing cells and the pulsation when a drug is acting thereon is captured via electric signals obtained from electrodes, and the properties of the change in potential are evaluated.
摘要翻译：本发明提供了一种药物效应评价装置，其能够在药物作用于表达hERG的细胞的同时对药物的作用进行片上评价。本发明还提供一种心肌毒性试验装置及其方法，其能够进行体外心肌毒性试验，其先前已在体内进行。脉动细胞群体和表达hERG的细胞（靶模型细胞）被适当地分离并排列在透明基底上，使得两个形成间隙连接。 hERG表达细胞设置在设置在透明基板上的透明电极上。将表达hERG的细胞暴露于含有药物的液体流中，使药物作用于其上。通过从电极获得的电信号捕获hERG表达细胞的正常脉动与药物作用于其上的脉动之间的差异，并评估电位变化的性质。

100. 发明申请

US20120088295A1 DEVICE FOR CONCENTRATING AND SEPARATING CELLS 有权
标题翻译：用于浓缩和分离细胞的装置
公开(公告)号：US20120088295A1
公开(公告)日：2012-04-12
申请号：US13138785
申请日：2010-03-31
申请人： Kenji Yasuda , Masahito Hayashi , Akihiro Hattori
发明人： Kenji Yasuda , Masahito Hayashi , Akihiro Hattori
IPC分类号： C12M1/42 , B03C5/02 , C12M1/00 , B01D43/00
CPC分类号： C12M47/04 , B01D21/283 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , B01L2400/0415 , B01L2400/0424 , B01L2400/0439
摘要： Provided is a device for concentrating and separating cells, which has a function for continuously concentrating cells; a function for then continuously arranging the concentrated cells in predetermined regions of a flow path; a function for simultaneously identifying shape and fluorescent emission in one-cell units on the basis of cell concentration and purification images, which serve to continuously separate and purify cells that have different properties in that they are either attracted to or repelled by an induction electrophoresis force of a predetermined frequency; and a function for identifying cells on the basis of this shape and fluorescent emission information and thereby separating and purifying the cells.
摘要翻译：提供了用于浓缩和分离细胞的装置，其具有用于连续浓缩细胞的功能; 然后连续地将浓缩的细胞排列在流路的预定区域中的功能; 基于细胞浓度和纯化图像同时鉴定单细胞单位形状和荧光发射的功能，其用于连续分离和纯化具有不同性质的细胞，因为它们被感应电泳力吸引或排斥的预定频率; 以及基于该形状和荧光发射信息鉴别细胞，从而分离和纯化细胞的功能。

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