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1. 发明申请

US20120068454A1 PIPE JOINT 有权
标题翻译：管接头
公开(公告)号：US20120068454A1
公开(公告)日：2012-03-22
申请号：US13320145
申请日：2010-05-07
申请人： Tamaki Hamaguchi , Tomoyuki Minami , Fumitaka Satoh , Takaaki Inotani , Fumikazu Ishibe , Satoru Araki , Youichi Uchiumi , Takuji Okiayu , Akihiro Hattori
发明人： Tamaki Hamaguchi , Tomoyuki Minami , Fumitaka Satoh , Takaaki Inotani , Fumikazu Ishibe , Satoru Araki , Youichi Uchiumi , Takuji Okiayu , Akihiro Hattori
IPC分类号： F16L55/00 , F16L19/00 , F16L21/00
CPC分类号： F16L25/0045 , F16L37/088 , F16L2201/10
摘要： A pipe joint comprising a pressing nut into which a flexible pipe is inserted, a joint body into which the pressing nut is partially inserted, a resilient means unlocked when pushed by a tip end of the flexible pipe, a sealing member, a retainer engaging the flexible pipe, and an engaging mechanism keeping the pressing nut at a predetermined position in the joint body; the engaging mechanism comprising a stop ring, an annular groove formed on an outer surface of the pressing nut for receiving the stop ring, pluralities of engaging grooves formed on an inner surface of the joint body for receiving the stop ring and communicating with each other; the stop ring engaging both of the annular groove and the first engaging groove before the connection of the flexible pipe is completed; and when the flexible pipe is pulled after completing the connection, the stop ring moving from the first engaging groove to the second engaging groove to draw the pressing nut from the joint body, so that the normal connection of the flexible pipe can be confirmed.
摘要翻译：一种管接头，包括一个柔性管插入其中的一个按压螺母，一个接合体，该压紧螺母部分插入该接头体中，一个由柔性管的顶端推动时解锁的弹性装置，一个密封件，柔性管，以及将按压螺母保持在接头体内的预定位置的接合机构; 所述接合机构包括止动环，形成在所述按压螺母的外表面上的用于接收所述止动环的环形槽，形成在所述接头本体的内表面上的用于接收所述止动环并且彼此连通的多个接合槽; 在柔性管连接之前完成环形槽与第一接合槽啮合的止动环; 并且当完成连接之后拉动柔性管时，止动环从第一接合槽移动到第二接合槽，以从接头本体拉出按压螺母，从而可以确认柔性管的正常连接。

2. 发明授权

US08008069B2 Apparatus and method for machining microchamber for cell culture 有权
标题翻译：用于细胞培养微孔加工的装置和方法
公开(公告)号：US08008069B2
公开(公告)日：2011-08-30
申请号：US10544799
申请日：2004-02-19
申请人： Akihiro Hattori , Kenji Yasuda
发明人： Akihiro Hattori , Kenji Yasuda
IPC分类号： C12M1/00 , C12M3/00 , C12M1/34
CPC分类号： C12M23/16 , B01J2219/00317 , B01J2219/00441 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/0063 , B01J2219/00637 , B01J2219/00743 , B01L3/5085 , B01L2200/12 , B01L2300/0819 , B01L2300/12 , B01L2300/163
摘要： This invention relates to a method and apparatus for process cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source. The absorption layer has absorbency in visible and infrared regions, and the gel-like material is a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions. The light source is monochromatic light in the specific wave length. The light source is disposed such that it irradiates on the absorption layer and/or gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on the transparent base plate.
摘要翻译：本发明涉及一种用于过程细胞培养的方法和装置。该装置包括一个微室，该微室包括不超过一个吸收层和至少一个凝胶层，依次层叠在透明基板上，在可见光和红外区域中没有明显的吸收性，以及至少一个光源。吸收层在可见光和红外区域具有吸收性，凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物，在室温下处于凝胶状态，具有吸收性特定波长的可见光和红外区域。光源是特定波长的单色光。光源被设置成使其照射在吸收层和/或凝胶层上，除了当没有设置吸收层时，每个由凝胶状材料构成的至少两层层叠在透明基板上。

3. 发明授权

US09182061B2 Pipe joint 有权
标题翻译：管接头
公开(公告)号：US09182061B2
公开(公告)日：2015-11-10
申请号：US13320145
申请日：2010-05-07
申请人： Tamaki Hamaguchi , Tomoyuki Minami , Fumitaka Satoh , Takaaki Inotani , Fumikazu Ishibe , Satoru Araki , Youichi Uchiumi , Takuji Okiayu , Akihiro Hattori
发明人： Tamaki Hamaguchi , Tomoyuki Minami , Fumitaka Satoh , Takaaki Inotani , Fumikazu Ishibe , Satoru Araki , Youichi Uchiumi , Takuji Okiayu , Akihiro Hattori
IPC分类号： F16L37/00 , F16L25/00 , F16L37/088
CPC分类号： F16L25/0045 , F16L37/088 , F16L2201/10
摘要： A pipe joint comprising a pressing nut into which a flexible pipe is inserted, a joint body into which the pressing nut is partially inserted, a resilient means unlocked when pushed by a tip end of the flexible pipe, a sealing member, a retainer engaging the flexible pipe, and an engaging mechanism keeping the pressing nut at a predetermined position in the joint body; the engaging mechanism comprising a stop ring, an annular groove formed on an outer surface of the pressing nut for receiving the stop ring, pluralities of engaging grooves formed on an inner surface of the joint body for receiving the stop ring and communicating with each other; the stop ring engaging both of the annular groove and the first engaging groove before the connection of the flexible pipe is completed; and when the flexible pipe is pulled after completing the connection, the stop ring moving from the first engaging groove to the second engaging groove to draw the pressing nut from the joint body, so that the normal connection of the flexible pipe can be confirmed.
摘要翻译：一种管接头，包括一个柔性管插入其中的一个按压螺母，一个接合体，该压紧螺母部分插入该接头体中，一个由柔性管的顶端推动时解锁的弹性装置，一个密封件，柔性管，以及将按压螺母保持在接头体内的预定位置的接合机构; 所述接合机构包括止动环，形成在所述按压螺母的外表面上的用于接收所述止动环的环形槽，形成在所述接头本体的内表面上的用于接收所述止动环并且彼此连通的多个接合槽; 在柔性管连接之前完成环形槽与第一接合槽啮合的止动环; 并且当完成连接之后拉动柔性管时，止动环从第一接合槽移动到第二接合槽，以从接头本体拉出按压螺母，从而可以确认柔性管的正常连接。

4. 发明授权

US09109197B2 Device for concentrating and separating cells 有权
标题翻译：用于浓缩和分离细胞的装置
公开(公告)号：US09109197B2
公开(公告)日：2015-08-18
申请号：US13138785
申请日：2010-03-31
申请人： Kenji Yasuda , Masahito Hayashi , Akihiro Hattori
发明人： Kenji Yasuda , Masahito Hayashi , Akihiro Hattori
IPC分类号： C12M1/34 , C12M3/00 , C12M1/00
CPC分类号： C12M47/04 , B01D21/283 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , B01L2400/0415 , B01L2400/0424 , B01L2400/0439
摘要： Provided is a device for concentrating and separating cells, which has a function for continuously concentrating cells; a function for then continuously arranging the concentrated cells in predetermined regions of a flow path; a function for simultaneously identifying shape and fluorescent emission in one-cell units on the basis of cell concentration and purification images, which serve to continuously separate and purify cells that have different properties in that they are either attracted to or repelled by an induction electrophoresis force of a predetermined frequency; and a function for identifying cells on the basis of this shape and fluorescent emission information and thereby separating and purifying the cells.
摘要翻译：提供了用于浓缩和分离细胞的装置，其具有用于连续浓缩细胞的功能; 然后连续地将浓缩的细胞排列在流路的预定区域中的功能; 基于细胞浓度和纯化图像同时鉴定单细胞单位形状和荧光发射的功能，其用于连续分离和纯化具有不同性质的细胞，因为它们被感应电泳力吸引或排斥的预定频率; 以及基于该形状和荧光发射信息鉴别细胞，从而分离和纯化细胞的功能。

5. 发明授权

US09023294B2 Cell analyzer 有权
标题翻译：细胞分析仪
公开(公告)号：US09023294B2
公开(公告)日：2015-05-05
申请号：US13261410
申请日：2011-02-24
申请人： Hideyuki Terazono , Kenji Yasuda , Masahito Hayashi , Hiroyuki Takei , Akihiro Hattori
发明人： Hideyuki Terazono , Kenji Yasuda , Masahito Hayashi , Hiroyuki Takei , Akihiro Hattori
IPC分类号： C12M3/00 , C12M1/00 , G01N1/31 , C12N1/06 , G01N1/40 , G01N15/14 , G01N21/64 , G01N1/34
CPC分类号： G01N1/31 , C12N1/066 , G01N1/34 , G01N1/4077 , G01N15/1459 , G01N15/1463 , G01N21/6408 , G01N21/6428 , G01N2001/4094 , G01N2015/149 , G01N2021/6482 , Y10T436/11
摘要： Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof.
摘要翻译：提供一种细胞浓缩和净化装置，其具有：连续浓缩细胞的功能; 随后随后将细胞连续地放置在通道的特定区域中的功能; 基于图像同时识别每个单个细胞的形状和荧光发射的功能; 以及基于与其形状和荧光发射有关的数据识别细胞，然后分离和纯化细胞的功能。

6. 发明申请

US20130314526A1 CELL ANALYSIS DEVICE 审中-公开
标题翻译：细胞分析装置
公开(公告)号：US20130314526A1
公开(公告)日：2013-11-28
申请号：US13882257
申请日：2011-09-16
申请人： Kenji Yasuda , Hideyuki Terazono , Hyonchol Kim , Masahito Hayashi , Akihiro Hattori , Kazuto Nishio , Tokuzo Arao , Yohei Miyagi , Takashi Ohtsu
发明人： Kenji Yasuda , Hideyuki Terazono , Hyonchol Kim , Masahito Hayashi , Akihiro Hattori , Kazuto Nishio , Tokuzo Arao , Yohei Miyagi , Takashi Ohtsu
IPC分类号： G06T7/00
CPC分类号： G06T7/0016 , G01N15/1475 , G01N15/1484 , G01N27/447 , G01N33/487 , G01N2015/1006 , G01N2015/1472 , G01N2015/149 , G01N2015/1493 , G01N2015/1497
摘要： Provided is a cell concentration/purification device having a function of successively locating cells in a specific area of a microchannel, and a function of sequentially capturing cell images by use of light from a plurality of monochromatic light sources on an image basis, and performing comparative analysis of the cell images to recognize individual cells based on information on the shape of the cells and an absorption spectral distribution of the cells or inside of the cells, thereby selectively separating/purifying the cells.
摘要翻译：本发明提供一种细胞浓度/净化装置，其具有将细胞连续定位在微通道的特定区域中的功能，以及通过使用来自多个单色光源的光以图像为基础顺序捕获细胞图像的功能，并进行比较基于关于细胞形状的信息和细胞的吸收光谱分布或细胞内部的信息来分析细胞图像以识别个体细胞，从而选择性地分离/纯化细胞。

7. 发明申请

US20130029407A1 CELL ANALYZER 有权
标题翻译：细胞分析仪
公开(公告)号：US20130029407A1
公开(公告)日：2013-01-31
申请号：US13261410
申请日：2011-02-24
申请人： Hideyuki Terazono , Kenji Yasuda , Masahito Hayashi , Hiroyuki Takei , Akihiro Hattori
发明人： Hideyuki Terazono , Kenji Yasuda , Masahito Hayashi , Hiroyuki Takei , Akihiro Hattori
IPC分类号： C12M1/42
CPC分类号： G01N1/31 , C12N1/066 , G01N1/34 , G01N1/4077 , G01N15/1459 , G01N15/1463 , G01N21/6408 , G01N21/6428 , G01N2001/4094 , G01N2015/149 , G01N2021/6482 , Y10T436/11
摘要： Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof.
摘要翻译：提供一种细胞浓缩和净化装置，其具有：连续浓缩细胞的功能; 随后随后将细胞连续地放置在通道的特定区域中的功能; 基于图像同时识别每个单个细胞的形状和荧光发射的功能; 以及基于与其形状和荧光发射有关的数据识别细胞，然后分离和纯化细胞的功能。

8. 发明申请

US20120088295A1 DEVICE FOR CONCENTRATING AND SEPARATING CELLS 有权
标题翻译：用于浓缩和分离细胞的装置
公开(公告)号：US20120088295A1
公开(公告)日：2012-04-12
申请号：US13138785
申请日：2010-03-31
申请人： Kenji Yasuda , Masahito Hayashi , Akihiro Hattori
发明人： Kenji Yasuda , Masahito Hayashi , Akihiro Hattori
IPC分类号： C12M1/42 , B03C5/02 , C12M1/00 , B01D43/00
CPC分类号： C12M47/04 , B01D21/283 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , B01L2400/0415 , B01L2400/0424 , B01L2400/0439
摘要： Provided is a device for concentrating and separating cells, which has a function for continuously concentrating cells; a function for then continuously arranging the concentrated cells in predetermined regions of a flow path; a function for simultaneously identifying shape and fluorescent emission in one-cell units on the basis of cell concentration and purification images, which serve to continuously separate and purify cells that have different properties in that they are either attracted to or repelled by an induction electrophoresis force of a predetermined frequency; and a function for identifying cells on the basis of this shape and fluorescent emission information and thereby separating and purifying the cells.
摘要翻译：提供了用于浓缩和分离细胞的装置，其具有用于连续浓缩细胞的功能; 然后连续地将浓缩的细胞排列在流路的预定区域中的功能; 基于细胞浓度和纯化图像同时鉴定单细胞单位形状和荧光发射的功能，其用于连续分离和纯化具有不同性质的细胞，因为它们被感应电泳力吸引或排斥的预定频率; 以及基于该形状和荧光发射信息鉴别细胞，从而分离和纯化细胞的功能。

9. 外观设计

USD620917S1 Interphone 有权
公开(公告)号：USD620917S1
公开(公告)日：2010-08-03
申请号：US29320258
申请日：2008-06-24
申请人： Akihiro Hattori , Katsuhiko Ibuki
设计人： Akihiro Hattori , Katsuhiko Ibuki

10. 发明申请

US20100092969A1 CELL ISOLATION METHOD, CELL TESTING METHOD AND REAGENT KIT THEREFOR 审中-公开
标题翻译：细胞分离方法，细胞检测方法及其试剂盒
公开(公告)号：US20100092969A1
公开(公告)日：2010-04-15
申请号：US12530154
申请日：2007-03-07
申请人： Atsushi Kira , Akihiro Hattori , Kenji Yasuda
发明人： Atsushi Kira , Akihiro Hattori , Kenji Yasuda
IPC分类号： C12Q1/68 , C12N5/00 , C12M1/00
CPC分类号： G01N33/56966 , C12N13/00 , G01N33/54326 , G01N33/57492
摘要： The present invention provides a cell isolation method, a cell screening process, and a reagent kit therefor. In the present invention, the recognition substance is a polynucleotide specifically bound to a particular antigen that exists on the surface of a specific cell. This recognition substance is made to bind with a magnet of paramagnetic material. The specific cell is obtained by mixing a sample and the magnet, recovering the magnet through the use of magnetic force, applying nuclease, digesting the polynucleotide bound to the particular antigen of a specific cell, and using magnetic force to remove the magnet. According to the present invention, loss of cell functionality during the classification process can be avoided when cell identification or isolation is necessary.
摘要翻译：本发明提供细胞分离方法，细胞筛选方法及其试剂盒。在本发明中，识别物质是特异性结合于特定细胞表面上的特定抗原的多核苷酸。该识别物质与顺磁材料的磁体结合。通过混合样品和磁体，通过使用磁力回收磁体，施加核酸酶，消化与特定细胞的特定抗原结合的多核苷酸，并使用磁力除去磁体，获得特定细胞。根据本发明，当需要小区识别或隔离时，可以避免分类过程中的小区功能的损失。

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