专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

91. 发明申请

US20090061416A1 Surfaces and methods for biosensor cellular assays 审中-公开
标题翻译：用于生物传感器细胞测定的表面和方法
公开(公告)号：US20090061416A1
公开(公告)日：2009-03-05
申请号：US12072599
申请日：2008-02-27
申请人： Ye Fang , Ann M. Ferrie , Elizabeth Tran , Jun Xi
发明人： Ye Fang , Ann M. Ferrie , Elizabeth Tran , Jun Xi
IPC分类号： C12Q1/70 , C12M1/00 , C12N11/00 , G01N33/569 , G01N33/53
CPC分类号： G01N33/54373 , G01N33/569
摘要： Disclosed is an apparatus for measuring ligand-induced cell activity as defined herein, the apparatus including: an optical biosensor having a contact surface including a compatibilizer zone, an optional surface modifier zone, and a live cell zone. The disclosure also provides a method of making the apparatus and methods for measuring ligand-induced live cell activity with the apparatus.
摘要翻译：本发明公开了一种如本文所定义的用于测量配体诱导的细胞活性的装置，该装置包括：光学生物传感器，其具有包括增容剂区，任选的表面改性区和活细胞区的接触表面。本公开还提供了一种制备用该装置测量配体诱导的活细胞活性的装置和方法的方法。

92. 发明申请

US20070238197A9 Fluorescent ligands for GPCR arrays 审中-公开
标题翻译： GPCR阵列的荧光配体
公开(公告)号：US20070238197A9
公开(公告)日：2007-10-11
申请号：US11363400
申请日：2006-02-27
申请人： Ye Fang , Yulong Hong , Jinlin Peng
发明人： Ye Fang , Yulong Hong , Jinlin Peng
IPC分类号： G01N33/543
CPC分类号： G01N33/582 , C07K7/083 , C07K14/63 , G01N33/6845 , G01N33/74 , G01N2333/63 , G01N2333/726
摘要： Microarrays employing a fluorescent ligand including a material having a binding affinity in the range of about 0.01 to about 25 nM, or about 0.1 to about 10 nM; a specificity to its cognate receptor in the range of about 50 to about 99%, or about 65 to about 99%; a cross-activity to other receptors of 0 to about 20%, or 0 to about 10%; a net charge per ligand of about −3 to about +5, or more preferably, about −2 to about +2 or most preferably for small compound ligands about −1 to about +2. The ligand may also have a hydrophobicity in the range of about 3 to about 55 minutes eluting time (as measured under specified eluting conditions). In some embodiments, the ligand includes fluorescently labeled motilin 1-16 labeled with Bodipy-TMR, rhodamine or Cy5-. Other embodiments include fluorescently labeled Cy5-naltrexone, Cy5-neurotensin 2-13, N-terminal labeled neurotensin 2-13 or lys-labeled labeled neurotensin 2-13.
摘要翻译：使用荧光配体的微阵列，其包括具有约0.01至约25nM或约0.1至约10nM范围内的结合亲和力的材料; 在约50至约99％，或约65至约99％的范围内对其同源受体的特异性; 与其他受体的交叉活性为0至约20％，或0至约10％; 每个配体的净电荷为约-3至约+5，或更优选约-2至约+2，或最优选约为-1至约+2的小化合物配体。配体还可以具有约3至约55分钟洗脱时间（在规定的洗脱条件下测量）的疏水性。在一些实施方案中，配体包括用Bodipy-TMR，罗丹明或Cy5-标记的荧光标记的胃动素1-16。其他实施方案包括荧光标记的Cy5-纳曲酮，Cy5-神经丝氨酸2-13，N-末端标记的神经丝氨酸蛋白酶2-13或lys-标记的标记的神经丝氨酸素2-13。

93. 发明申请

US20070031884A1 Universal readout for target identification using biological microarrays 失效
标题翻译：使用生物微阵列进行目标识别的通用读数
公开(公告)号：US20070031884A1
公开(公告)日：2007-02-08
申请号：US11546266
申请日：2006-10-11
申请人： Ye Fang
发明人： Ye Fang
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： C40B30/04 , B01J2219/00315 , B01J2219/00387 , B01J2219/00497 , B01J2219/00511 , B01J2219/00536 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00639 , B01J2219/00659 , B01J2219/00677 , B01J2219/00689 , B01J2219/00693 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01J2219/0074 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G01N2035/00158 , Y10S436/807 , C12Q2537/161
摘要： A method and apparatus for implementing the method is provided. The method involves performing an indirect competitive binding assay on a microarray to identify biological or chemical targets and screen for compounds of interest. The microarray comprises a common ligand located among membrane-, lipid- or protein-associated active binding sites. The method takes advantage of known or well-characterized binding kinetics, and steric interference between biological or chemicals targets of interest and universal readout units for different binding sites within the limited confines of a microspot. The biological targets, chemicals or organisms can specifically bind to target-binding sites, while the universal readout unit binds to the ligands in the microspot.
摘要翻译：提供了一种用于实现该方法的方法和装置。该方法包括在微阵列上进行间接竞争性结合测定以鉴定生物或化学靶标并筛选感兴趣的化合物。微阵列包含位于膜 - ，脂质 - 或蛋白质相关的活性结合位点之间的共同配体。该方法利用已知或良好表征的结合动力学，以及感兴趣的生物学或化学物质目标之间的空间干扰以及在微量有限范围内的不同结合位点的通用读出单元。生物靶标，化学物质或生物体可以特异性地结合靶结合位点，而通用读出单元与微孔中的配体结合。

94. 发明授权

US06977155B2 Arrays of biological membranes and methods and use thereof 失效
标题翻译：生物膜阵列及其用途
公开(公告)号：US06977155B2
公开(公告)日：2005-12-20
申请号：US09854786
申请日：2001-05-14
申请人： Joydeep Lahiri , Ye Fang , Steven J. Jonas , Peter J. Kalal , Wei Wang
发明人： Joydeep Lahiri , Ye Fang , Steven J. Jonas , Peter J. Kalal , Wei Wang
IPC分类号： C40B40/10 , G01N33/543 , G01N33/566 , G01N33/68 , G01N33/53 , G01N33/552
CPC分类号： G01N33/5438 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00725 , B01J2219/0074 , B82Y30/00 , C40B40/10 , G01N33/566 , G01N33/6872 , G01N2333/726 , G01N2500/02
摘要： The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel or a receptor tyrosine kinase.
摘要翻译：本发明克服了与现有技术阵列相关的问题和缺点，提供了一种阵列，其包括与基底表面相关联的多个生物膜微孔，该生物膜微孔可以在水溶液环境中生产，使用和储存，而不是在环境中在环境或受控的湿度下暴露于空气。优选地，生物膜微点包含膜结合蛋白。最优选地，膜结合蛋白是G蛋白偶联受体，离子通道或受体酪氨酸激酶。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式