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91. 发明申请

US20090233875A1 GLYCOLIPIDS AND ANALOGUES THEREOF AS ANTIGENS FOR NK T CELLS 审中-公开
公开(公告)号：US20090233875A1
公开(公告)日：2009-09-17
申请号：US12388415
申请日：2009-02-18
申请人： Moriya TSUJI , David D. Ho , Chi-Huey Wong , Doug Wu , Masakazu Fujio
发明人： Moriya TSUJI , David D. Ho , Chi-Huey Wong , Doug Wu , Masakazu Fujio
IPC分类号： A61K31/7004 , C07H15/04 , A61P37/02
CPC分类号： C07H15/06 , C07H15/00 , C07H15/18 , C07H15/26
摘要： This invention relates to immunogenic compounds which may serve as ligands for NKT (natural killer T) cells and to methods of use thereof in modulating immune responses.

92. 发明申请

US20090232831A1 METHODS AND COMPOSITIONS FOR THE TREATMENT OR PREVENTION OF HUMAN IMMUNODEFICIENCY VIRUS INFECTION 审中-公开
标题翻译：治疗或预防人类免疫缺陷病毒感染的方法和组合
公开(公告)号：US20090232831A1
公开(公告)日：2009-09-17
申请号：US12338987
申请日：2008-12-18
申请人： Chi-Huey Wong , Sheng-Kai Wang , Pi-Hui Liang
发明人： Chi-Huey Wong , Sheng-Kai Wang , Pi-Hui Liang
IPC分类号： A61K39/42 , A61K31/715 , C07H1/00 , C12Q1/70
CPC分类号： A61K31/715 , A61K39/00 , G01N33/56988 , G01N33/6854 , G01N2400/00 , G01N2500/04
摘要： A conserved cluster of oligomannose glycans on gp120 has been identified as the epitope recognized by the broadly HIV-1-neutralizing monoclonal antibody 2G12. Oligomannose glycans are also the ligands for DC-SIGN, a C-type lectin found on the surface of dendritic cells. Multivalency is fundamental for carbohydrate-protein interactions, and mimicking of the high glycan density on the virus surface has become essential for designing carbohydrate-based HIV vaccines and antiviral agents. Synthesis of oligomannose dendrons, which display multivalent oligomannoses in high density, and characterize their interaction with 2G12 and DC-SIGN by a glycan microarray binding assay is disclosed. These glycodendrons inhibit the binding of gp120 to 2G12 and recombinant dimeric DC-SIGN with IC50 in the nanomolar range. A second-generation Man9 dendron was identified as a potential immunogen for HIV vaccine development and as a potential antiviral agent.
摘要翻译：已经鉴定了gp120上寡聚糖单糖聚糖的保守簇，被广泛的HIV-1中和单克隆抗体2G12识别为表位。寡聚甘露糖聚糖也是DC-SIGN的配体，DC-SIGN是树突细胞表面上发现的C型凝集素。多值是碳水化合物 - 蛋白质相互作用的基础，并且模仿病毒表面上的高聚糖密度对于设计基于碳水化合物的HIV疫苗和抗病毒剂已变得至关重要。公开了通过聚糖微阵列结合测定法显示高密度多价寡糖单糖的寡甘露糖树突的合成，并表征其与2G12和DC-SIGN的相互作用。这些甘草酮抑制gp120与2G12的结合和重组二聚体DC-SIGN，其IC50在纳摩尔范围。第二代Man9树突被鉴定为HIV疫苗开发的潜在免疫原，并被认为是潜在的抗病毒剂。

93. 发明申请

US20080292644A1 Compositions and methods for identifying response targets and treating flavivirus infection responses 有权
标题翻译：用于鉴定反应目标和治疗黄病毒感染反应的组合物和方法
公开(公告)号：US20080292644A1
公开(公告)日：2008-11-27
申请号：US12079576
申请日：2008-03-27
申请人： Shie-Liang Hsieh , Chi-Huey Wong , Tsui-Ling Hsu , Szu-Ting Chen
发明人： Shie-Liang Hsieh , Chi-Huey Wong , Tsui-Ling Hsu , Szu-Ting Chen
IPC分类号： A61K39/395 , G01N33/68 , C12Q1/70 , A01K67/027 , C12Q1/68
CPC分类号： C07K16/2851 , A61K2039/505 , C07K2317/56 , C07K2317/76 , C07K2319/30 , G01N2333/18 , G01N2333/185 , G01N2500/02 , Y02A50/386 , Y02A50/388 , Y02A50/39 , Y02A50/394
摘要： Cellular receptors are identified that induce plasma leakage and other negative effects when infected with flaviviruses, such as dengue virus or Japanese encephamyelitis virus. Using fusion proteins disclosed herein, the receptors to which a pathogen, such as flavivirus, binds via glycan binding are determined. Once the receptors are determined, the effect of binding to a particular receptor may be determined, wherein targeting of the receptors causing a particular symptom may be targeted by agents that interrupt binding of the pathogen to the receptor. Accordingly, in the case of dengue virus and Japanese encephamyelitis virus, TNF-α is released when the pathogen binds to the DLVR1/CLEC5A receptor. Interrupting the DLVR1/CLEC5A receptor with monoclonal antibodies reduced TNF-α secretion without affecting secretion of cytokines responsible for viral clearance thereby increasing survival rates in infected mice from nil to around 50%.
摘要翻译：鉴定当感染黄病毒如登革热病毒或日本脑炎病毒时诱导血浆渗漏和其它负面影响的细胞受体。使用本文公开的融合蛋白，确定病原体（例如黄病毒）通过聚糖结合结合的受体。一旦确定了受体，就可以确定与特定受体结合的作用，其中导致特定症状的受体的靶向可能被阻断病原体与受体结合的试剂所靶向。因此，在登革热病毒和日本脑炎病毒的情况下，当病原体与DLVR1 / CLEC5A受体结合时，TNF-α被释放。用单克隆抗体中断DLVR1 / CLEC5A受体降低TNF-α分泌，而不影响负责病毒清除的细胞因子分泌，从而将感染小鼠的存活率从零增加到约50％。

94. 发明申请

US20080241856A1 Glycoproteomic probes for fluorescent imaging of fucosylated glycans in vivo 有权
标题翻译：用于体内岩藻糖基化聚糖荧光成像的糖蛋白质组学探针
公开(公告)号：US20080241856A1
公开(公告)日：2008-10-02
申请号：US12079228
申请日：2008-03-24
申请人： Chi-Huey Wong , Tsui-Ling Hsu , Sarah R. Hanson , Masaaki Sawa
发明人： Chi-Huey Wong , Tsui-Ling Hsu , Sarah R. Hanson , Masaaki Sawa
IPC分类号： G01N33/53 , C12P19/44 , C12Q1/00
CPC分类号： G01N33/582 , G01N1/30 , G01N2400/00
摘要： The disclosure provides a method of labeling of cellular glycans bearing azide groups via a fluorescent labeling technique based on Cu(I)-catalyzed [3+2]cycloaddition (click activation) of a probe comprising an alkynyl group. The method entails generating a fluorescent probe from a nonfluorescent precursor, 4-ethynyl-N-ethyl-1,8-naphthalimide, by Cu(I)-catalyzed [3+2]cycloaddition of the alkyne group of the probe with an azido-modified sugar. The disclosure further provides a method of incorporating an azido-containing fucose analog into glycoconjugates via the fucose salvage pathway. The disclosure provides a method of fluorescent visualization of fucosylated cells by flow cytometry when cells treated with 6-azidofucose are labeled with the click-activated fluorogenic probe or biotinylated alkyne. A method of visualizing the intracellular localization of fucosylated glycoconjugates by fluorescence microscopy is also disclosed.
摘要翻译：本公开提供了通过基于包含炔基的探针的Cu（I）催化的[3 + 2]环加成（点击活化）的荧光标记技术来标记携带叠氮基的细胞聚糖的方法。该方法需要通过Cu（I）催化的[3 + 2]环加成的探针的炔基与非荧光前体4-乙炔基-N-乙基-1,8-萘酰亚胺的生成荧光探针，改性糖。本公开进一步提供了通过岩藻糖补救途径将含叠氮基岩藻糖类似物掺入糖缀合物的方法。本公开内容提供了当用6-叠氮基聚糖处理的细胞用点击激活的荧光探针或生物素化的炔被标记时，流式细胞仪荧光显示岩藻糖基化细胞的方法。还公开了通过荧光显微镜观察岩藻糖基化糖缀合物的细胞内定位的方法。

95. 发明申请

US20070213278A1 Arrays with cleavable linkers 审中-公开
标题翻译：具有可切割接头的数组
公开(公告)号：US20070213278A1
公开(公告)日：2007-09-13
申请号：US11645273
申请日：2006-12-22
申请人： Chi-Huey Wong , Dennis Burton , Ian Wilson
发明人： Chi-Huey Wong , Dennis Burton , Ian Wilson
IPC分类号： A61K31/70 , G01N33/53
CPC分类号： G01N33/54353 , A61K31/716 , C07H3/06 , G01N33/56972 , G01N33/56988 , G01N33/57415 , G01N2400/02 , G01N2400/14
摘要： The invention provides arrays of molecules where the molecules (e.g., glycans) are attached to the arrays by cleavable linkers. The invention also provides methods for using these arrays, methods for identifying the structural elements of molecules bound to these arrays by using the cleavable linkers, especially the structural elements that are important for binding to test samples. The invention further provides methods for evaluating whether test samples and test molecules can bind to distinct glycans on the arrays and useful glycans identified using the methods and arrays provided herein.
摘要翻译：本发明提供了分子阵列，其中分子（例如，聚糖）通过可切割接头连接到阵列上。本发明还提供了使用这些阵列的方法，通过使用可切割接头，特别是结合测试样品重要的结构元件来鉴定与这些阵列结合的分子的结构元件的方法。本发明还提供了用于评估测试样品和测试分子是否可以结合阵列上的不同聚糖和使用本文提供的方法和阵列鉴定的有用聚糖的方法。

96. 发明授权

US06423834B1 KDO aldolase and condensation reactions employed therewith 失效
标题翻译： KDO醛缩酶和与其一起使用的缩合反应
公开(公告)号：US06423834B1
公开(公告)日：2002-07-23
申请号：US09247164
申请日：1999-02-09
申请人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
IPC分类号： C07H1500
CPC分类号： C12P19/02 , C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , Y10S435/822
摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.
摘要翻译：公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。另外，保护形式的KDO醛缩酶产物，例如，六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

97. 发明授权

US6080563A Method for synthesizing 2-ketoaldonic acids 失效
标题翻译：合成2-酮戊二酸的方法
公开(公告)号：US6080563A
公开(公告)日：2000-06-27
申请号：US88941
申请日：1998-06-02
申请人： Chi-Huey Wong
发明人： Chi-Huey Wong
IPC分类号： C07H1/08 , C07H7/027 , C07H7/033 , C12N9/88 , C12P7/58 , C12P9/00 , C12P19/02 , C12P7/40 , C07H1/00 , C12P9/88
CPC分类号： C12N9/88 , C07H1/08 , C07H7/027 , C07H7/033 , C12P19/02 , C12P7/58 , C12P9/00
摘要： 2-Ketoaldonic acid is synthesized by aldolase condensation reaction involving pyruvate and an aldose acceptor in the presence of excess pyruvate. After the reaction is substantially complete, the excess pyruvate is removed from the reaction mixture by treatment with pyruvate decarboxylase.
摘要翻译：在过量的丙酮酸存在下，2-酮糖醛酸通过醛缩酶缩合反应合成，涉及丙酮酸和醛糖受体。反应基本完成后，通过用丙酮酸脱羧酶处理从反应混合物中除去过量的丙酮酸。

98. 发明授权

US5919673A One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates 失效
标题翻译：使用3'-磷酸腺苷-5'-磷酸硫酸盐和再循环的磷酸化腺苷中间体的一锅酶硫酸化方法
公开(公告)号：US5919673A
公开(公告)日：1999-07-06
申请号：US408774
申请日：1995-03-22
申请人： Chi-Huey Wong , Chun-Hung Lin , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Chun-Hung Lin , Gwo-Jenn Shen
IPC分类号： C12N9/10 , C12N9/12 , C12P11/00 , C12P19/26 , C12P19/30 , C12P19/04 , C12P19/32 , C12P33/00
CPC分类号： C12N9/13 , C12N9/1205 , C12N9/1241 , C12P11/00 , C12P19/26 , C12P19/30
摘要： A process for the enzymatic synthase of sulfate esters is disclosed in which intermediate phosphorylated adenosine compounds are recycled to minimize enzyme inhibition. Exemplary enzymes include ATP sulfurylase, APS kinase and a sulfotransferase.
摘要翻译：公开了一种硫酸酯酶合成酶的方法，其中中间磷酸化腺苷化合物被循环以使酶抑制最小化。示例性的酶包括ATP硫酸化酶，APS激酶和磺基转移酶。

99. 发明授权

US5869316A Biologically pure culture of aureobacterium barkeri KDO-37-2 失效
标题翻译：生物纯培养的巴氏杆菌KDO-37-2
公开(公告)号：US5869316A
公开(公告)日：1999-02-09
申请号：US767182
申请日：1996-12-16
申请人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
IPC分类号： C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , C12P19/02 , C12N1/20
CPC分类号： C12P19/02 , C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , Y10S435/822
摘要： Aureobacterium barkeri strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.
摘要翻译：公开了分离自巴斯德氏菌杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。另外，保护形式的KDO醛缩酶产物，例如，六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

100. 发明授权

US5795749A Use of 2-deoxyribose-5-phosphate aldolase to prepare 2-deoxyfucose, analogues and derivatives 失效
标题翻译：使用2-脱氧核糖-5-磷酸醛缩酶制备2-脱氧岩藻糖，类似物和衍生物
公开(公告)号：US5795749A
公开(公告)日：1998-08-18
申请号：US416999
申请日：1995-04-05
申请人： Chi-Huey Wong , Harrie J. M. Gijsen
发明人： Chi-Huey Wong , Harrie J. M. Gijsen
IPC分类号： C12P19/02 , C07H1/00 , C12P11/00 , C12P19/00
CPC分类号： C12P19/02
摘要： Processes using 2-deoxyribose-5-phosphate aldolase (DERA) are described for the preparation of 2-deoxyfucose and related compounds. In one embodiment, DERA is used to catalyze the condensation of acetaldehyde as donor and a 2(R)-hydroxy-3-(hydroxy or mercapto)-propionaldehyde derivative to form a 2-deoxysugar whose hydroxyls have the configuration of fucose. In another embodiment, DERA is used to catalyze the condensation of two moles of acetaldehyde as donor and one mole of a 2-substituted acetaldehyde acceptor to form a 2,4,6-trideoxyhexose via a 4-substituted-3-hydroxybutanal intermediate.
摘要翻译：描述了使用2-脱氧核糖-5-磷酸醛缩酶（DERA）的方法用于制备2-脱氧岩藻糖和相关化合物。在一个实施方案中，DERA用于催化作为供体的乙醛和2（R） - 羟基-3-（羟基或巯基） - 丙醛衍生物的缩合形成其羟基具有岩藻糖构型的2-脱氧糖。在另一个实施方案中，DERA用于催化2摩尔乙醛供体和1摩尔2-取代乙醛受体的缩合，以通过4-取代-3-羟基丁醛中间体形成2,4,6-三脱氧己糖。

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