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    • 95. 发明申请
    • ANTIBODY CAPABLE OF RECOGNIZING HLA CLASS I
    • 抗体鉴定能力的抗体I
    • US20120244142A1
    • 2012-09-27
    • US13497545
    • 2010-09-24
    • Naoki KimuraKiyoshi Habu
    • Naoki KimuraKiyoshi Habu
    • A61K39/395A61P35/00C07K16/18
    • C07K16/2833C07K2317/24C07K2317/41C07K2317/524C07K2317/626C07K2317/73
    • An objective of the present invention is to provide antibodies that recognize HLA class I and have significant cell death-inducing activity. To achieve the above objective, first, the present inventors immunized mice with soluble HLA-A to which a FLAG tag is attached. Four days after the final immunization, spleen cells from the mice were fused with mouse myeloma cells. Then, hybridoma screening was carried out using the cell aggregation-inducing activity and cell death-inducing activity as an index. Thus, the monoclonal antibody F17B1 that has strong cell death-inducing activity was selected. The sequences of the H chain and L chain variable regions of the mouse monoclonal antibody F17B1 were determined to humanize this antibody. Then, the humanized anti-HLA class I antibody H2-G1d_L1-k0 was assessed for cell death induction. The result showed that H2-G1d_L1-k0 suppressed the growth of the IM9 cell line in a concentration dependent manner.
    • 本发明的目的是提供识别HLA I类并具有显着的细胞死亡诱导活性的抗体。 为了实现上述目的,首先,本发明人用附着有FLAG标签的可溶性HLA-A免疫小鼠。 最后免疫4天后,将来自小鼠的脾细胞与小鼠骨髓瘤细胞融合。 然后,使用细胞聚集诱导活性和细胞死亡诱导活性作为指标进行杂交瘤筛选。 因此,选择具有强细胞死亡诱导活性的单克隆抗体F17B1。 确定小鼠单克隆抗体F17B1的H链和L链可变区的序列以使该抗体人源化。 然后,评估人源化抗HLA I类抗体H2-G1d_L1-k0的细胞死亡诱导。 结果表明,H2-G1d_L1-k0以浓度依赖性方式抑制IM9细胞系的生长。