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91. 发明申请

US20060229223A1 Lipolytic enzyme variant 有权
标题翻译：脂解酶变体
公开(公告)号：US20060229223A1
公开(公告)日：2006-10-12
申请号：US11453670
申请日：2006-06-15
申请人： Stefan Minning , Jesper Vind , Sanne Glad , Steffen Danielsen , Kim Borch
发明人： Stefan Minning , Jesper Vind , Sanne Glad , Steffen Danielsen , Kim Borch
IPC分类号： C11D3/386 , C07H21/04 , C12P21/06 , C12P7/64 , C12P7/62 , C12N9/20 , C12N1/18 , C12N15/74 , C11D7/42
CPC分类号： C12N9/20
摘要： The present invention relates to lipolytic enzyme variants with improved thermostability are obtained by substituting certain specified amino acid residues in a fungal lipolytic enzyme. The thermostable lipolytic enzyme variants are useful, e.g., for controlling pitch troubles in a process for the production of mechanical pulp or a paper-making process using mechanical pulp.
摘要翻译：本发明涉及具有改善的热稳定性的脂肪分解酶变体，其通过在真菌脂肪分解酶中取代某些特定氨基酸残基获得。热稳定性脂肪分解酶变体是有用的，例如用于控制用于生产机械纸浆的方法中的沥青混合料或使用机械纸浆的造纸方法。

92. 发明申请

US20060228446A1 Variant lipolytic ensymes 有权
标题翻译：变种脂肪分解酶
公开(公告)号：US20060228446A1
公开(公告)日：2006-10-12
申请号：US10556511
申请日：2004-04-29
申请人： Kim Borch , Luise Erlandsen , Jesper Vind , Allan Svendsen , Christel Jorgensen
发明人： Kim Borch , Luise Erlandsen , Jesper Vind , Allan Svendsen , Christel Jorgensen
IPC分类号： A21D8/02 , C07H21/04 , C12P21/06 , C12N9/16 , C12N15/74 , C12N1/18
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译：本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。多肽可以特别地对极性脂质具有活性。

93. 发明申请

US20050255544A1 Lipolytic enzyme variants and method for their production 审中-公开
标题翻译：脂肪分解酶变体及其生产方法
公开(公告)号：US20050255544A1
公开(公告)日：2005-11-17
申请号：US10495597
申请日：2003-01-16
申请人： Allan Svendsen , Jesper Vind , Hans Heldt-Hansen , Luise Erlandsen
发明人： Allan Svendsen , Jesper Vind , Hans Heldt-Hansen , Luise Erlandsen
IPC分类号： C12N9/20 , C12P21/06 , C12N1/14 , C12N15/74
CPC分类号： C12N9/20 , C12Y301/01003
摘要： The inventors have developed a method using protein engineering to produce lipolytic enzymes having a relatively high activity for one ester bond in an amphiphilic substrate with two lipophilic groups) and a relatively low activity for the ester bond in an amphiphilic substrate with one lipophilic group, e.g. a relatively high phospholipase activity and a relatively low lysophospholipase activity.
摘要翻译：本发明人开发了一种使用蛋白质工程的方法来生产对于具有两个亲脂基团的两亲底物中的一个酯键具有相对较高活性的脂肪分解酶），并且在具有一个亲油基团的两亲底物中具有较低的酯键活性。相对较高的磷脂酶活性和较低的溶血磷脂酶活性。

94. 发明授权

US06964944B1 Chemically modified lipolytic enzyme 失效
公开(公告)号：US06964944B1
公开(公告)日：2005-11-15
申请号：US10019156
申请日：2000-06-02
申请人： Thomas Hønger Callisen , Shamkant Anant Patkar , Allan Svendsen , Jesper Vind
发明人： Thomas Hønger Callisen , Shamkant Anant Patkar , Allan Svendsen , Jesper Vind
IPC分类号： C12N15/09 , A21D2/26 , A21D8/02 , C11D3/386 , C12N9/20 , C12N9/96 , C12S9/00
CPC分类号： C11D3/38627 , C12N9/20
摘要： Lipolytic enzymes are chemically modified by covalently linking one or more (particularly 1–3) hydrophobic groups to the enzyme molecule. The chemical modification improves the performance of the lipolytic enzyme, e.g., in baking or in detergents.

95. 发明授权

US06767701B1 Methods of constructing and screening a DNA library of interest in filamentous fungal cells 有权
标题翻译：在丝状真菌细胞中构建和筛选感兴趣的DNA文库的方法
公开(公告)号：US06767701B1
公开(公告)日：2004-07-27
申请号：US09426038
申请日：1999-10-25
申请人： Jesper Vind
发明人： Jesper Vind
IPC分类号： C07H2104
CPC分类号： C12N15/80 , C12N9/20 , C12N15/1034
摘要： A method of constructing and screening a library of polynucleotide sequences of interest in filamentous fungal cells by use of an episomal replicating AMA1-based plasmid vector, thus achieving a high frequency of transformation and a stable and standard uniformly high level of gene expression.
摘要翻译：通过使用附加型复制的基于AMA1的质粒载体构建和筛选丝状真菌细胞中的多核苷酸序列文库的方法，从而实现高频率的转化和稳定和标准均匀的高水平的基因表达。

96. 发明授权

US06645749B2 Lipolytic enzyme 失效
公开(公告)号：US06645749B2
公开(公告)日：2003-11-11
申请号：US09931401
申请日：2001-08-16
申请人： Jesper Vind
发明人： Jesper Vind
IPC分类号： C12N920
CPC分类号： C11D3/38627 , A21D8/042 , C12N9/20
摘要： The present invention is directed to phospholipase from Fusarium solani (MUCL38667) and analogues thereof, nucleic acid constructs, recombinant expression vectors, and recombinant host comprising the nucleic acid sequences encoding the phospholipase from Fusarium solani (MUCL38667) and analogues thereof, methods of making same, and method of using the phosopholipases of the present invention in baking methods, baking compositions and detergent compositions.

97. 发明授权

US06624129B1 Lipase variant 有权
标题翻译：脂肪酶变体
公开(公告)号：US06624129B1
公开(公告)日：2003-09-23
申请号：US09630250
申请日：2000-08-01
申请人： Kim Borch , Jesper Vind , Allan Svendsen , Dorte Aaby Halkier , Shamkant Anant Patkar , Kirsten Bojsen
发明人： Kim Borch , Jesper Vind , Allan Svendsen , Dorte Aaby Halkier , Shamkant Anant Patkar , Kirsten Bojsen
IPC分类号： C12N916
CPC分类号： C11D1/83 , C11D1/02 , C11D3/38627 , C12N9/20
摘要： The present invention relates to lipase variants having at least 90% identity to the wild-type lipase derived from Humicola lanuginosa strain DSM 4109 and having a certain distribution of electrically charged amino acids. The present invention also relates to detergents comprising such lipases.
摘要翻译：本发明涉及与源自Humicola lanuginosa菌株DSM 4109的野生型脂肪酶具有至少90％同一性且具有一定分布的带电氨基酸的脂肪酶变体。本发明还涉及包含这种脂肪酶的洗涤剂。

98. 发明授权

US06518042B1 Process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells 失效
标题翻译：使用参与丝状真菌细胞错配修复系统的新型克隆基因在丝状真菌细胞中制备DNA文库的方法
公开(公告)号：US06518042B1
公开(公告)日：2003-02-11
申请号：US09512250
申请日：2000-02-24
申请人： Torben Vedel Borchert , Lars Christiansen , Jesper Vind
发明人： Torben Vedel Borchert , Lars Christiansen , Jesper Vind
IPC分类号： C12P2102
CPC分类号： C07K14/38 , C12N15/80
摘要： A process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells.
摘要翻译：使用涉及丝状真菌细胞的错配修复系统的新的克隆基因在丝状真菌细胞中制备DNA文库的方法。

99. 发明授权

US06352851B1 Glucoamylase variants 有权
标题翻译：葡糖淀粉酶变体
公开(公告)号：US06352851B1
公开(公告)日：2002-03-05
申请号：US09351814
申请日：1999-07-12
申请人： Bjarne Roenfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
发明人： Bjarne Roenfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
IPC分类号： C12N934
CPC分类号： C12N9/2428 , C12P19/14 , C12P19/20 , Y02E50/17
摘要： The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.
摘要翻译：本发明涉及亲本真菌葡糖淀粉酶的变体，其使用糖基底显示出改善的热稳定性和/或增加的比活性。

100. 发明授权

US6159687A Methods for generating recombined polynucleotides 失效
标题翻译：产生重组多核苷酸的方法
公开(公告)号：US6159687A
公开(公告)日：2000-12-12
申请号：US040697
申请日：1998-03-18
申请人： Jesper Vind
发明人： Jesper Vind
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1093 , C12N15/1027
摘要： A method for in vitro construction of a library of recombined homologous polynucleotides from a number of different starting DNA templates and primers by induced template shifts during an polynucleotide synthesis is described, wherebyA. extended primers are synthesized bya) denaturing the DNA templatesb) annealing primers to the templates,c) extending the said primers by use of a polymerase,d) stop the synthesis, ande) separate the extended primers from the templates,B. a template shift is induced bya) isolating the extended primers from the templates and repeating steps A.b) to A.e) using the extended primers as both primers and templates, orb) repeating steps A.b) to A.e),C. this process is terminated after an appropriate number of cycles of process steps A. and B.a), A. and B.b), or combinations thereof.Optionally the polynucleotides are amplified in a standard PCR reaction with specific primers to selectively amplify homologous polynucleotides of interest.
摘要翻译：描述了在多核苷酸合成期间通过诱导的模板位移从许多不同的起始DNA模板和引物体外构建重组的同源多核苷酸文库的方法，其中A.通过a）使DNA模板变性来合成扩展的引物b）将引物退火至模板，c）通过使用聚合酶扩增所述引物，d）停止合成，和e）将延伸的引物与模板分离。B.通过以下步骤诱导模板位移：a）模板和重复步骤Ab）至Ae），使用扩展引物作为引物和模板，或b）重复步骤Ab）至Ae），C.该过程在经过适当数量的工艺步骤A和B的循环之后终止），A.和Bb）或其组合。任选地，在与特异性引物的标准PCR反应中扩增多核苷酸以选择性扩增目的同源多核苷酸。

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