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    • 9. 发明申请
    • CARRIER FOR ANTIBODY PURIFICATION, MANUFACTURING METHOD FOR SAME, AND APPLICATION FOR SAME
    • 用于抗体纯化的载体,其制造方法及其应用
    • US20150152195A1
    • 2015-06-04
    • US14406728
    • 2013-06-11
    • DAISO CO., LTD.
    • Masahiro IwakuraKiyonori HirotaSeiji Otaka
    • C07K17/14C07K1/22B01D15/38C07K14/31
    • C07K17/14B01D15/3809B01J20/286B01J20/3204B01J20/3217B01J20/3274C07K1/22C07K14/31C07K16/065C07K2317/92
    • An object of the present invention is to provide a carrier on which Protein A has been immobilized, the Protein A having a specific amino acid sequence that permits the desorption of antibodies under mild pH conditions (specifically, pH4.0 to 5.5) in which many antibodies do not undergo acid modification; and a manufacturing method for the carrier. An immobilization carrier (excluding an immobilization carrier having a monolith structure) on which a protein is adsorbed by electrostatic interaction, the protein consisting of an amino acid sequence represented by the general formula: R1-R2-R3-R4-R5-R6, wherein the part represented by R1-R2-R3 is used for immobilization on the immobilization carrier, wherein: the sequence represents a sequence from the amino-terminal side towards the carboxyl-terminal side; the sequence of the R2 part is the sequence of a Protein A mutant as a protein to be immobilized or a sequence in which 1 to 3 units of the sequence thereof were linked together, the Protein A mutant having characteristics of strongly binding to an antibody under neutral conditions and dissociating with the antibody bound under neutral conditions under weakly acidic conditions of pH 4.0 to 5.5.
    • 本发明的目的是提供一种其上固定有蛋白A的载体,具有特定氨基酸序列的蛋白A,其允许在温和的pH条件(具体地,pH4.0至5.5)下解吸抗体,其中许多 抗体不经过酸修饰; 和载体的制造方法。 通过静电相互作用吸附有蛋白质的固定载体(不包括具有整料结构的固定化载体),由通式R1-R2-R3-R4-R5-R6表示的氨基酸序列组成的蛋白质,其中 由R1-R2-R3表示的部分用于固定化载体上,其中:该序列表示从氨基末端侧到羧基末端侧的序列; R2部分的序列是作为要固定的蛋白质的蛋白A突变体的序列或其中1至3个单元的序列连接在一起的序列,具有与抗体强烈结合的特征的蛋白A突变体 中性条件,并在pH 4.0至5.5的弱酸性条件下,在中性条件下与抗体结合解离。