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1. 发明授权

US5066462A Analytical element for measuring enzyme activity 失效
标题翻译：测定酶活性的分析元素
公开(公告)号：US5066462A
公开(公告)日：1991-11-19
申请号：US401494
申请日：1989-08-29
申请人： Kazuya Kawasaki , Mitsutoshi Tanaka , Yoshikazu Amano , Harumi Katsuyama
发明人： Kazuya Kawasaki , Mitsutoshi Tanaka , Yoshikazu Amano , Harumi Katsuyama
IPC分类号： G01N33/50 , C12Q1/00 , C12Q1/26 , C12Q1/48 , C12Q1/52 , G01N33/52
CPC分类号： C12Q1/48 , G01N33/525 , Y10S435/805
摘要： A dry-type analytical element suitable for measuring the activity of alanine aminotransferase in a liquid sample, characterized by incorporating a dye capable of absorbing the electromagnetic waves of 400 to 500 nm into at least one water-permeable layer. The coloring sensitivity of the dry-type analytical element does not increase substantially even under a fluorescent light, and thereby, an accurate measured value can easily be obtained.
摘要翻译：适用于测量液体样品中丙氨酸氨基转移酶活性的干型分析元件，其特征在于将能够吸收400-500nm电磁波的染料掺入到至少一个透水层中。即使在荧光下，干式分析元件的着色灵敏度也不会显着增加，从而可以容易地获得准确的测量值。

2. 发明授权

US5508173A Analytical element for measurements of enzyme activity 失效
标题翻译：酶活性测定用分析元素
公开(公告)号：US5508173A
公开(公告)日：1996-04-16
申请号：US373885
申请日：1995-01-17
申请人： Yoshikazu Amano , Kazuya Kawasaki , Harumi Katsuyama
发明人： Yoshikazu Amano , Kazuya Kawasaki , Harumi Katsuyama
IPC分类号： G01N31/22 , C12Q1/00 , G01N33/52 , C12Q1/28 , C12Q1/26 , C12Q1/52
CPC分类号： G01N33/525 , G01N33/526
摘要： A multilayer analytical element for measuring enzyme activity in a liquid sample such as a body fluid which is less affected by intrinsic pyruvic acid contained in the liquid sample is disclosed. The analytical element comprises a support, a reagent layer, an optionally provided other layer, and a porous spreading layer. The reagent layer and/or the optionally provided other layer contain pyruvic acid oxidase. The characteristic feature of the analytical element lies in that the spreading layer has a specific void volume in the range of 3-15 .mu.l/cm.sup.2.
摘要翻译：公开了一种用于测量液体样品中的酶活性的多层分析元件，所述液体样品例如体液中较少受到液体样品中所含的固有丙酮酸的影响。分析元件包括载体，试剂层，任选提供的其它层和多孔扩散层。试剂层和/或任选提供的其它层含有丙酮酸氧化酶。分析元件的特征在于扩散层具有在3-15μml/ cm 2范围内的特定空隙体积。

3. 发明授权

US4503145A Quantitative analysis film 失效
标题翻译：定量分析膜
公开(公告)号：US4503145A
公开(公告)日：1985-03-05
申请号：US352970
申请日：1982-02-26
申请人： Harumi Katsuyama , Yoshikazu Amano , Asaji Kondo
发明人： Harumi Katsuyama , Yoshikazu Amano , Asaji Kondo
IPC分类号： G01N33/66 , C12Q1/00 , C12Q1/26 , C12Q1/28 , C12Q1/48 , C12Q1/52 , G01N31/22 , G01N33/92 , G01N33/52
CPC分类号： C12Q1/52 , C12Q1/26 , Y10S435/805
摘要： An analysis film for assaying transaminase activity comprises a reagent layer containing pyruvic acid oxidase, a combination of a hydrogen donor and a coupler--which is capable of forming a cationic dye--and an anionic polymer. The cationic dye formed by the reaction with a color indicator composition for detecting hydrogen peroxide is fixed with the anionic polymer, thereby mobilization and diffusion of the cationic dye being prevented so that the analysis film achieves high detection sensitivity and high accuracy.
摘要翻译：用于测定转氨酶活性的分析膜包括含有丙酮酸氧化酶，氢供体和能够形成阳离子染料的成色剂的组合的试剂层和阴离子聚合物。通过与用于检测过氧化氢的颜色指示剂组合物的反应形成的阳离子染料用阴离子聚合物固定，从而阻止阳离子染料的移动和扩散，使得分析膜实现高检测灵敏度和高精度。

4. 发明授权

US4478942A Quantitative analysis film and a method for colorimetric analysis using the same 失效
标题翻译：定量分析膜和使用其的比色分析方法
公开(公告)号：US4478942A
公开(公告)日：1984-10-23
申请号：US352969
申请日：1982-02-26
申请人： Harumi Katsuyama , Yoshikazu Amano , Asaji Kondo
发明人： Harumi Katsuyama , Yoshikazu Amano , Asaji Kondo
IPC分类号： G01N33/50 , C12Q1/28 , G01N31/00 , G01N31/22 , G01N33/52 , G01N33/70 , G01N33/92
CPC分类号： G01N33/523 , C12Q1/28 , C12Q2326/00 , C12Q2326/96 , Y10S435/805 , Y10S436/904 , Y10T436/206664
摘要： A quantitative analysis film comprising a reagent layer comprising a cationic dye-forming color indicator for detecting hydrogen peroxide and an anionic polymer. By the use of such a color indicator and an anionic polymer in combination, a cationic dye is formed; the formed cationic dye is then bound and fixed to the anionic polymer to provide an intensified color. A method for the colorimetric assay of hydrogen peroxide or a precursor thereof with high sensitivity and high efficiency is also disclosed.
摘要翻译：一种定量分析膜，其包含试剂层，其包含用于检测过氧化氢的阳离子染料形成颜色指示剂和阴离子聚合物。通过使用这种颜色指示剂和阴离子聚合物组合，形成阳离子染料; 然后将形成的阳离子染料结合并固定到阴离子聚合物以提供增强的颜色。还公开了高灵敏度和高效率的过氧化氢或其前体的比色测定方法。

5. 发明授权

US5006458A Analytical element having a polyacrylamide or derivative to minimize background for assaying enzyme activity 失效
标题翻译：具有聚丙烯酰胺或衍生物的分析元件以最小化测定酶活性的背景
公开(公告)号：US5006458A
公开(公告)日：1991-04-09
申请号：US135670
申请日：1987-12-21
申请人： Keiko Kato , Shigeki Kageyama , Yoshikazu Amano , Fuminori Arai , Harumi Katsuyama
发明人： Keiko Kato , Shigeki Kageyama , Yoshikazu Amano , Fuminori Arai , Harumi Katsuyama
IPC分类号： G01N31/22 , C12Q1/00 , C12Q1/32 , G01N33/52
CPC分类号： G01N33/525 , Y10S435/805 , Y10S436/81 , Y10S436/904
摘要： A dry-type analytical element suitable for measuring enzyme activity of in a liquid sample, characterized by incorporating a polyacrylamide, polymethacrylamide or their derivatives into at least one water-permeable layer. The background concentration of the dry-type analytical element exhibits minimal increase even under a fluorescent light, and allows an accurate measured value to be easily obtained.

6. 发明授权

US4889797A Dry analytical element having a spread control area for assaying enzyme activity 失效
标题翻译：具有用于测定酶活性的扩散控制区的干燥分析元件
公开(公告)号：US4889797A
公开(公告)日：1989-12-26
申请号：US54432
申请日：1987-05-26
申请人： Yoshikazu Amano , Shigeki Kageyama , Harumi Katsuyama
发明人： Yoshikazu Amano , Shigeki Kageyama , Harumi Katsuyama
IPC分类号： C12Q1/32 , C12Q1/40 , C12Q1/42 , C12Q1/48 , G01N33/52
CPC分类号： G01N33/52 , C12Q1/32 , C12Q1/40 , C12Q1/42 , C12Q1/48 , Y10S435/805 , Y10S435/81 , Y10S436/808 , Y10S436/81
摘要： A dry analytical element for assaying an enzyme in a liquid is disclosed, comprising a support having provided thereon at least a porous liquid-spreading layer composed of water non-absorbent fibers, wherein said porous liquid-spreading layer contains a substrate for an enzyme to be assayed and a hydrophilic polymer in an amount effective to decrease a spreading area by at least 20%. The analytical element exhibits high sensitivity and high reproducibility.
摘要翻译：公开了一种用于测定液体中的酶的干燥分析元件，其包括至少在由非水吸收性纤维构成的多孔液体扩散层上提供的支撑体，其中所述多孔液体扩散层含有用于酶的底物并且将亲水性聚合物的量有效地将扩散面积减少至少20％。分析元件显示出高灵敏度和高再现性。

7. 发明授权

US5496708A Reagent composition for quantitative analysis of inorganic phosphorous and dry analysis element utilizing the same 失效
标题翻译：用于定量分析无机磷和干分析元素的试剂组合物
公开(公告)号：US5496708A
公开(公告)日：1996-03-05
申请号：US259616
申请日：1994-06-14
申请人： Kazuya Kawasaki , Yoshikazu Amano , Osamu Seshimoto , Kiyoshi Yamada
发明人： Kazuya Kawasaki , Yoshikazu Amano , Osamu Seshimoto , Kiyoshi Yamada
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/48 , G01N31/22
CPC分类号： C12Q1/48 , Y10S435/805 , Y10S435/97
摘要： A reagent composition for the quantitative analysis of inorganic phosphorus, composted of xanthosine, purine nucleoside phosphorylase, xanthine oxidase and a color-former. The color-former is a precursor for forming a dye in the presence of hydrogen peroxide and peroxidase. The reagent composition of the invention is improved in storage stability and has a wider determination range. Also provided is a dry analysis element composed of reagent layer containing the inventive reagent composition.
摘要翻译：用于定量分析无机磷的试剂组合物，堆肥的黄嘌呤，嘌呤核苷磷酸化酶，黄嘌呤氧化酶和成色剂。成色剂是在过氧化氢和过氧化物酶存在下形成染料的前体。本发明的试剂组合物的贮存稳定性提高，判定范围更广。还提供了由包含本发明试剂组合物的试剂层组成的干分析元件。

8. 发明授权

US07105128B2 Dry analytical element using water-soluble indicator for colorimetry 有权
公开(公告)号：US07105128B2
公开(公告)日：2006-09-12
申请号：US10281564
申请日：2002-10-28
申请人： Hideaki Tanaka , Yoshihide Iwaki , Yoshiki Sakaino , Kazuya Kawasaki , Yoshikazu Amano
发明人： Hideaki Tanaka , Yoshihide Iwaki , Yoshiki Sakaino , Kazuya Kawasaki , Yoshikazu Amano
IPC分类号： G01N31/22
CPC分类号： G01N33/525 , G01N33/84
摘要： This invention relates to a dry analytical element using a water-soluble indicator which improves measuring accuracy, which comprises a water-impermeable support, a water-permeable layer and a spreading layer having a function to spread a liquid uniformly, wherein a water-soluble indicator for colorimetry is incorporated into the s preading layer.

9. 发明授权

US08658758B2 Process for producing aliphatic polyester reduced in residual cyclic ester content 有权
标题翻译：制备脂肪族聚酯的方法减少了残留的环酯含量
公开(公告)号：US08658758B2
公开(公告)日：2014-02-25
申请号：US10593230
申请日：2005-03-17
申请人： Hiroyuki Sato , Fuminori Kobayashi , Yukichika Kawakami , Kazuyuki Yamane , Yoshikazu Amano , Takashi Sato
发明人： Hiroyuki Sato , Fuminori Kobayashi , Yukichika Kawakami , Kazuyuki Yamane , Yoshikazu Amano , Takashi Sato
IPC分类号： C08G63/08
CPC分类号： C08G63/90 , C08G63/06 , C08G63/80
摘要： In the production of an aliphatic polyester by ring-opening polymerization of a cyclic ester, at least a latter period of polymerization is proceeded with by way of solid-phase polymerization, and the resultant aliphatic polyester is subjected to removal of residual cyclic ester. As a result, an aliphatic polyester with a minimized content of residual monomer is obtained.
摘要翻译：在通过环状酯的开环聚合生产脂肪族聚酯时，至少通过固相聚合进行聚合的后期，并将所得的脂族聚酯除去残留的环状酯。结果，得到残留单体含量最小的脂肪族聚酯。

10. 发明授权

US5592290A Method for correcting instrumental error of spectroscope of optical analyzer 失效
标题翻译：光学分析仪分光仪器误差校正方法
公开(公告)号：US5592290A
公开(公告)日：1997-01-07
申请号：US358486
申请日：1994-11-14
申请人： Fuminori Arai , Yoshikazu Amano , Kiyoshi Yamada
发明人： Fuminori Arai , Yoshikazu Amano , Kiyoshi Yamada
IPC分类号： G01N33/52 , G01N21/27 , G01N21/78 , G01N21/86 , G01N31/22 , G01J3/00
CPC分类号： G01N21/8483 , G01N21/274
摘要： A method for correcting an instrumental error due to wavelength error of a spectroscope of an optical analyzer for measuring an optical density reflected from or transmitted through a dry analysis element. One standard color plate having a standard optical density OD.sub.ST is measured by an optical analyzer to be corrected to obtain an measured value OD.sub.M. A measured optical density OD.sub.S of the element applied with a sample through the optical analyzer to be corrected is corrected by using a ratio of OD.sub.M /OD.sub.ST to obtain a corrected measured value OD.sub.C of the sample. The standard color plate to be used contains an indicator dye contained in the non-reacted dry analysis element or a dye having an absorption spectrum same as or similar to that of the indicator dye in the measurement wavelength range. Another correction method in consideration of a layer coefficient of a dry analysis element is also provided.
摘要翻译：一种用于校正由用于测量从干分析元件反射或透过干光分析元件的光密度的光学分析仪的分光镜引起的波长误差的仪器误差的方法。通过光学分析器测量具有标准光密度ODST的一个标准色板以进行校正，以获得测量值ODM。通过使用ODM / ODST的比例来校正通过待校正的光学分析器施加样品的元件的测量光密度ODS，以获得样品的校正测量值ODC。所使用的标准色板包含未反应干燥分析元件中所含的指示剂染料或在测量波长范围内具有与指示剂染料相同或类似的吸收光谱的染料。还提供了考虑干分析元件的层系数的另一校正方法。

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