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1. 发明申请

US20120225424A1 Device and Method for Electroporation-Based Delivery of Molecules Into Cells and Dynamic Monitoring of Cell Responses 有权
标题翻译：用于基于电穿孔的分子递送到细胞中的装置和方法以及细胞应答的动态监测
公开(公告)号：US20120225424A1
公开(公告)日：2012-09-06
申请号：US13474355
申请日：2012-05-17
申请人： Yama A. Abassi , Xiaobo Wang , Josephine Atienza , Xiao Xu , Junquan Xu
发明人： Yama A. Abassi , Xiaobo Wang , Josephine Atienza , Xiao Xu , Junquan Xu
IPC分类号： G01N27/02 , G01N21/00 , C12M1/42 , G01N21/64
CPC分类号： C12N15/87 , C12M35/02 , G01N15/12 , G01N33/48728
摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.
摘要翻译：本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

2. 发明授权

US08206903B2 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses 有权
标题翻译：分子进入细胞的基于电穿孔的装置和方法以及细胞反应的动态监测
公开(公告)号：US08206903B2
公开(公告)日：2012-06-26
申请号：US11286882
申请日：2005-11-23
申请人： Xiaobo Wang , Yama A. Abassi , Josephine Atienza , Xiao Xu , Junquan Xu
发明人： Xiaobo Wang , Yama A. Abassi , Josephine Atienza , Xiao Xu , Junquan Xu
IPC分类号： C12Q1/68
CPC分类号： C12N15/87 , C12M35/02 , G01N15/12 , G01N33/48728
摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.
摘要翻译：本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

3. 发明授权

US08921041B2 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses 有权
标题翻译：分子进入细胞的基于电穿孔的装置和方法以及细胞反应的动态监测
公开(公告)号：US08921041B2
公开(公告)日：2014-12-30
申请号：US13474355
申请日：2012-05-17
申请人： Xiaobo Wang , Yama A. Abassi , Josephine Atienza , Xiao Xu , Junquan Xu
发明人： Xiaobo Wang , Yama A. Abassi , Josephine Atienza , Xiao Xu , Junquan Xu
IPC分类号： C12Q1/68 , C12M1/42 , C12N15/87
CPC分类号： C12N15/87 , C12M35/02 , G01N15/12 , G01N33/48728
摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.
摘要翻译：本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

4. 发明申请

US20120295253A1 Dynamic Monitoring of Activation of Receptor Tyrosine Kinase (RTK) in Living Cells Using Real-Time Microelectronic Cell Sensing Technology 有权
标题翻译：动态监测活体细胞中受体酪氨酸激酶（RTK）的激活使用实时微电子细胞感测技术
公开(公告)号：US20120295253A1
公开(公告)日：2012-11-22
申请号：US13569156
申请日：2012-08-07
申请人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiaobo Wang
发明人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiaobo Wang
IPC分类号： C12Q1/68 , C12Q1/48 , G01N33/53
CPC分类号： G01N33/5041 , C12Q1/002 , G01N27/021 , G01N33/5014 , G01N33/5438 , G01N2333/4716 , G01N2333/705 , G01N2333/91205 , G01N2500/04 , G01N2500/10
摘要： Methods for identifying a compound capable of interacting with a Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance, adding test cells expressing a RTK to the device, measuring first impedances, adding a compound to at least one compound well and adding a vehicle control at least one control well, measuring second impedances of the compound well and the control well, determining the change in the impedance for the compound well and the one control well, comparing the change in impedance between the compound well and the control well, and identifying the compound interacts with the RTK if the comparison demonstrates a significant difference between the change in impedance.
摘要翻译：用于鉴定能够与受体酪氨酸激酶（RTK）相互作用的化合物的方法，包括提供能够测量细胞 - 基底阻抗的装置，将表达RTK的测试细胞加入到装置中，测量第一阻抗，将化合物加到至少一种化合物并且添加至少一个控制井的车辆控制，测量化合物井和对照井的第二阻抗，确定化合物井和一个对照井的阻抗变化，比较化合物井和化合物井之间的阻抗变化如果比较表明阻抗变化之间存在显着差异，则控制阱和识别化合物与RTK相互作用。

5. 发明授权

US08916357B2 Dynamic monitoring of activation of receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology 有权
标题翻译：使用实时微电子传感技术动态监测活细胞中受体酪氨酸激酶（RTK）的活化
公开(公告)号：US08916357B2
公开(公告)日：2014-12-23
申请号：US13569156
申请日：2012-08-07
申请人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiaobo Wang
发明人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiaobo Wang
IPC分类号： C12Q1/37 , G01N33/50 , C12Q1/00 , G01N33/543 , C12M1/42
CPC分类号： G01N33/5041 , C12Q1/002 , G01N27/021 , G01N33/5014 , G01N33/5438 , G01N2333/4716 , G01N2333/705 , G01N2333/91205 , G01N2500/04 , G01N2500/10
摘要： Methods for identifying a compound capable of interacting with a Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance, adding test cells expressing a RTK to the device, measuring first impedances, adding a compound to at least one compound well and adding a vehicle control to at least one control well, measuring second impedances of the compound well and the control well, determining the change in the impedance for the compound well and the one control well, comparing the change in impedance between the compound well and the control well, and identifying the compound interacts with the RTK if the comparison demonstrates a significant difference between the change in impedance.
摘要翻译：用于鉴定能够与受体酪氨酸激酶（RTK）相互作用的化合物的方法，包括提供能够测量细胞 - 基底阻抗的装置，将表达RTK的测试细胞加入到装置中，测量第一阻抗，将化合物加到至少一种化合物并且向至少一个控制井添加车辆控制，测量化合物井和对照井的第二阻抗，确定化合物井和一个对照井的阻抗变化，比较化合物井之间的阻抗变化和控制井，并且如果比较显示阻抗变化之间的显着差异，则识别化合物与RTK相互作用。

6. 发明申请

US20060121446A1 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses 有权
标题翻译：分子进入细胞的基于电穿孔的装置和方法以及细胞反应的动态监测
公开(公告)号：US20060121446A1
公开(公告)日：2006-06-08
申请号：US11286882
申请日：2005-11-23
申请人： Yama Abassi , Xiaobo Wang , Josephine Atienza , Xiao Xu , Junquan Xu
发明人： Yama Abassi , Xiaobo Wang , Josephine Atienza , Xiao Xu , Junquan Xu
IPC分类号： C12Q1/00 , C12N15/86
CPC分类号： C12N15/87 , C12M35/02 , G01N15/12 , G01N33/48728
摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.
摘要翻译：本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

7. 发明授权

US08263375B2 Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology 有权
标题翻译：使用实时微电子传感技术动态监测活细胞中G蛋白偶联受体（GPCR）和受体酪氨酸激酶（RTK）的活化
公开(公告)号：US08263375B2
公开(公告)日：2012-09-11
申请号：US11198831
申请日：2005-08-04
申请人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiabo Wang
发明人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiabo Wang
IPC分类号： C12N13/00 , C07H31/04
CPC分类号： G01N33/5041 , C12Q1/002 , G01N27/021 , G01N33/5014 , G01N33/5438 , G01N2333/4716 , G01N2333/705 , G01N2333/91205 , G01N2500/04 , G01N2500/10
摘要： The present application includes systems and methods for identifying a compound capable of interacting with a G-Protein Coupled Receptor (GPCR) or Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance operably connected to an impedance analyzer, adding test cells expressing a GPCR or a RTK to wells of the device, measuring first impedances of the wells and optionally determining first cell indices from the first impedances, adding a compound to at least one well containing test cells to form at least one compound well and adding a vehicle control to at least another well containing test cells to form at least one control well, measuring second impedances of the compound well and the control well and optionally determining second cell indices from the second impedances, determining the change in the impedance or cell index for the compound well and the one control well, comparing the change in impedance or cell index between the compound well and the control well, and identifying the compound interacts with the GPCR or RTK if the comparison demonstrates a significant difference between the change in impedance or cell index of the compound well and the control well.
摘要翻译：本申请包括用于鉴定能够与G-蛋白偶联受体（GPCR）或受体酪氨酸激酶（RTK）相互作用的化合物的系统和方法，包括提供能够测量可操作地连接到阻抗分析仪的细胞 - 基底阻抗的装置，将表达GPCR或RTK的测试细胞测量到所述装置的孔中，测量所述孔的第一阻抗并任选地从所述第一阻抗确定第一细胞指数，向至少一个含有测试细胞的孔中加入化合物以形成至少一种化合物，以及向至少另一个含有测试单元的井添加车辆控制以形成至少一个控制井，测量化合物井和控制井的第二阻抗，并且可选地从第二阻抗确定第二细胞指数，确定阻抗或细胞的变化指数为复合井和一个对照孔，比较了阻抗或细胞指数的变化如果比较表明化合物井和对照孔的阻抗变化或细胞指数之间存在显着差异，则鉴定化合物与GPCR或RTK相互作用。

8. 发明授权

US07732127B2 Dynamic monitoring of cell adhesion and spreading using the RT-CES system 有权
标题翻译：使用RT-CES系统动态监测细胞粘附和扩散
公开(公告)号：US07732127B2
公开(公告)日：2010-06-08
申请号：US11235938
申请日：2005-09-27
申请人： Xiaobo Wang , Yama Abassi , Josephine Atienza , Xiao Xu
发明人： Xiaobo Wang , Yama Abassi , Josephine Atienza , Xiao Xu
IPC分类号： C12Q1/00 , C12N5/00
CPC分类号： G01N29/036 , G01N2291/0257
摘要： The present invention includes devices and methods for dynamically monitoring cell adhesion and cell spreading. Cells are added to a microelectronic cell sensor array operably connected to an impedance analyzer. The device also includes a coating including biological molecule or organic compound capable of interacting with the cell. Cell adhesion and cell mobility is determined by detecting changes in impedance and comparing impedance or cell index values between samples.
摘要翻译：本发明包括用于动态监测细胞粘附和细胞扩散的装置和方法。将细胞添加到可操作地连接到阻抗分析器的微电子单元传感器阵列。该装置还包括包含能够与细胞相互作用的生物分子或有机化合物的涂层。通过检测阻抗变化并比较样品之间的阻抗或细胞指数值来确定细胞粘附和细胞迁移率。

9. 发明申请

US20060120204A1 Dynamic monitoring of cell adhesion and spreading using the RT-CES system 有权
标题翻译：使用RT-CES系统动态监测细胞粘附和扩散
公开(公告)号：US20060120204A1
公开(公告)日：2006-06-08
申请号：US11235938
申请日：2005-09-27
申请人： Yama Abassi , Josephine Atienza , Xiao Xu , Xiaobo Wang
发明人： Yama Abassi , Josephine Atienza , Xiao Xu , Xiaobo Wang
IPC分类号： G11C8/02
CPC分类号： G01N29/036 , G01N2291/0257
摘要： The present invention includes devices and methods for dynamically monitoring cell adhesion and cell spreading. Cells are added to a microelectronic cell sensor array operably connected to an impedance analyzer. The device also includes a coating including biological molecule or organic compound capable of interacting with the cell. Cell adhesion and cell mobility is determined by detecting changes in impedance and comparing impedance or cell index values between samples.
摘要翻译：本发明包括用于动态监测细胞粘附和细胞扩散的装置和方法。将细胞添加到可操作地连接到阻抗分析器的微电子单元传感器阵列。该装置还包括包含能够与细胞相互作用的生物分子或有机化合物的涂层。通过检测阻抗变化并比较样品之间的阻抗或细胞指数值来确定细胞粘附和细胞迁移率。

10. 发明申请

US20060050596A1 Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology 有权
标题翻译：使用实时微电子传感技术动态监测活细胞中G蛋白偶联受体（GPCR）和受体酪氨酸激酶（RTK）的活化
公开(公告)号：US20060050596A1
公开(公告)日：2006-03-09
申请号：US11198831
申请日：2005-08-04
申请人： Yama Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiabo Wang
发明人： Yama Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiabo Wang
IPC分类号： G11C8/00
CPC分类号： G01N33/5041 , C12Q1/002 , G01N27/021 , G01N33/5014 , G01N33/5438 , G01N2333/4716 , G01N2333/705 , G01N2333/91205 , G01N2500/04 , G01N2500/10
摘要： The present application includes systems and methods for identifying a compound capable of interacting with a G-Protein Coupled Receptor (GPCR) or Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance operably connected to an impedance analyzer, adding test cells expressing a GPCR or a RTK to wells of the device, measuring first impedances of the wells and optionally determining first cell indices from the first impedances, adding a compound to at least one well containing test cells to form at least one compound well and adding a vehicle control to at least another well containing test cells to form at least one control well, measuring second impedances of the compound well and the control well and optionally determining second cell indices from the second impedances, determining the change in the impedance or cell index for the compound well and the one control well, comparing the change in impedance or cell index between the compound well and the control well, and identifying the compound interacts with the GPCR or RTK if the comparison demonstrates a significant difference between the change in impedance or cell index of the compound well and the control well.
摘要翻译：本申请包括用于鉴定能够与G-蛋白偶联受体（GPCR）或受体酪氨酸激酶（RTK）相互作用的化合物的系统和方法，包括提供能够测量可操作地连接到阻抗分析仪的细胞 - 基底阻抗的装置，将表达GPCR或RTK的测试细胞测量到所述装置的孔中，测量所述孔的第一阻抗并任选地从所述第一阻抗确定第一细胞指数，向至少一个含有测试细胞的孔中加入化合物以形成至少一种化合物，以及向至少另一个含有测试单元的井添加车辆控制以形成至少一个控制井，测量化合物井和控制井的第二阻抗，并且可选地从第二阻抗确定第二细胞指数，确定阻抗或细胞的变化指数为复合井和一个对照孔，比较了阻抗或细胞指数的变化如果比较表明化合物井和对照孔的阻抗变化或细胞指数之间存在显着差异，则鉴定化合物与GPCR或RTK相互作用。

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