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1. 发明申请

US20120225424A1 Device and Method for Electroporation-Based Delivery of Molecules Into Cells and Dynamic Monitoring of Cell Responses 有权
标题翻译：用于基于电穿孔的分子递送到细胞中的装置和方法以及细胞应答的动态监测
公开(公告)号：US20120225424A1
公开(公告)日：2012-09-06
申请号：US13474355
申请日：2012-05-17
申请人： Yama A. Abassi , Xiaobo Wang , Josephine Atienza , Xiao Xu , Junquan Xu
发明人： Yama A. Abassi , Xiaobo Wang , Josephine Atienza , Xiao Xu , Junquan Xu
IPC分类号： G01N27/02 , G01N21/00 , C12M1/42 , G01N21/64
CPC分类号： C12N15/87 , C12M35/02 , G01N15/12 , G01N33/48728
摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.
摘要翻译：本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

2. 发明授权

US08206903B2 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses 有权
标题翻译：分子进入细胞的基于电穿孔的装置和方法以及细胞反应的动态监测
公开(公告)号：US08206903B2
公开(公告)日：2012-06-26
申请号：US11286882
申请日：2005-11-23
申请人： Xiaobo Wang , Yama A. Abassi , Josephine Atienza , Xiao Xu , Junquan Xu
发明人： Xiaobo Wang , Yama A. Abassi , Josephine Atienza , Xiao Xu , Junquan Xu
IPC分类号： C12Q1/68
CPC分类号： C12N15/87 , C12M35/02 , G01N15/12 , G01N33/48728
摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.
摘要翻译：本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

3. 发明授权

US08921041B2 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses 有权
标题翻译：分子进入细胞的基于电穿孔的装置和方法以及细胞反应的动态监测
公开(公告)号：US08921041B2
公开(公告)日：2014-12-30
申请号：US13474355
申请日：2012-05-17
申请人： Xiaobo Wang , Yama A. Abassi , Josephine Atienza , Xiao Xu , Junquan Xu
发明人： Xiaobo Wang , Yama A. Abassi , Josephine Atienza , Xiao Xu , Junquan Xu
IPC分类号： C12Q1/68 , C12M1/42 , C12N15/87
CPC分类号： C12N15/87 , C12M35/02 , G01N15/12 , G01N33/48728
摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.
摘要翻译：本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

4. 发明申请

US20120295253A1 Dynamic Monitoring of Activation of Receptor Tyrosine Kinase (RTK) in Living Cells Using Real-Time Microelectronic Cell Sensing Technology 有权
标题翻译：动态监测活体细胞中受体酪氨酸激酶（RTK）的激活使用实时微电子细胞感测技术
公开(公告)号：US20120295253A1
公开(公告)日：2012-11-22
申请号：US13569156
申请日：2012-08-07
申请人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiaobo Wang
发明人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiaobo Wang
IPC分类号： C12Q1/68 , C12Q1/48 , G01N33/53
CPC分类号： G01N33/5041 , C12Q1/002 , G01N27/021 , G01N33/5014 , G01N33/5438 , G01N2333/4716 , G01N2333/705 , G01N2333/91205 , G01N2500/04 , G01N2500/10
摘要： Methods for identifying a compound capable of interacting with a Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance, adding test cells expressing a RTK to the device, measuring first impedances, adding a compound to at least one compound well and adding a vehicle control at least one control well, measuring second impedances of the compound well and the control well, determining the change in the impedance for the compound well and the one control well, comparing the change in impedance between the compound well and the control well, and identifying the compound interacts with the RTK if the comparison demonstrates a significant difference between the change in impedance.
摘要翻译：用于鉴定能够与受体酪氨酸激酶（RTK）相互作用的化合物的方法，包括提供能够测量细胞 - 基底阻抗的装置，将表达RTK的测试细胞加入到装置中，测量第一阻抗，将化合物加到至少一种化合物并且添加至少一个控制井的车辆控制，测量化合物井和对照井的第二阻抗，确定化合物井和一个对照井的阻抗变化，比较化合物井和化合物井之间的阻抗变化如果比较表明阻抗变化之间存在显着差异，则控制阱和识别化合物与RTK相互作用。

5. 发明授权

US08916357B2 Dynamic monitoring of activation of receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology 有权
标题翻译：使用实时微电子传感技术动态监测活细胞中受体酪氨酸激酶（RTK）的活化
公开(公告)号：US08916357B2
公开(公告)日：2014-12-23
申请号：US13569156
申请日：2012-08-07
申请人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiaobo Wang
发明人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiaobo Wang
IPC分类号： C12Q1/37 , G01N33/50 , C12Q1/00 , G01N33/543 , C12M1/42
CPC分类号： G01N33/5041 , C12Q1/002 , G01N27/021 , G01N33/5014 , G01N33/5438 , G01N2333/4716 , G01N2333/705 , G01N2333/91205 , G01N2500/04 , G01N2500/10
摘要： Methods for identifying a compound capable of interacting with a Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance, adding test cells expressing a RTK to the device, measuring first impedances, adding a compound to at least one compound well and adding a vehicle control to at least one control well, measuring second impedances of the compound well and the control well, determining the change in the impedance for the compound well and the one control well, comparing the change in impedance between the compound well and the control well, and identifying the compound interacts with the RTK if the comparison demonstrates a significant difference between the change in impedance.
摘要翻译：用于鉴定能够与受体酪氨酸激酶（RTK）相互作用的化合物的方法，包括提供能够测量细胞 - 基底阻抗的装置，将表达RTK的测试细胞加入到装置中，测量第一阻抗，将化合物加到至少一种化合物并且向至少一个控制井添加车辆控制，测量化合物井和对照井的第二阻抗，确定化合物井和一个对照井的阻抗变化，比较化合物井之间的阻抗变化和控制井，并且如果比较显示阻抗变化之间的显着差异，则识别化合物与RTK相互作用。

6. 发明授权

US08263375B2 Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology 有权
标题翻译：使用实时微电子传感技术动态监测活细胞中G蛋白偶联受体（GPCR）和受体酪氨酸激酶（RTK）的活化
公开(公告)号：US08263375B2
公开(公告)日：2012-09-11
申请号：US11198831
申请日：2005-08-04
申请人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiabo Wang
发明人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiabo Wang
IPC分类号： C12N13/00 , C07H31/04
CPC分类号： G01N33/5041 , C12Q1/002 , G01N27/021 , G01N33/5014 , G01N33/5438 , G01N2333/4716 , G01N2333/705 , G01N2333/91205 , G01N2500/04 , G01N2500/10
摘要： The present application includes systems and methods for identifying a compound capable of interacting with a G-Protein Coupled Receptor (GPCR) or Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance operably connected to an impedance analyzer, adding test cells expressing a GPCR or a RTK to wells of the device, measuring first impedances of the wells and optionally determining first cell indices from the first impedances, adding a compound to at least one well containing test cells to form at least one compound well and adding a vehicle control to at least another well containing test cells to form at least one control well, measuring second impedances of the compound well and the control well and optionally determining second cell indices from the second impedances, determining the change in the impedance or cell index for the compound well and the one control well, comparing the change in impedance or cell index between the compound well and the control well, and identifying the compound interacts with the GPCR or RTK if the comparison demonstrates a significant difference between the change in impedance or cell index of the compound well and the control well.
摘要翻译：本申请包括用于鉴定能够与G-蛋白偶联受体（GPCR）或受体酪氨酸激酶（RTK）相互作用的化合物的系统和方法，包括提供能够测量可操作地连接到阻抗分析仪的细胞 - 基底阻抗的装置，将表达GPCR或RTK的测试细胞测量到所述装置的孔中，测量所述孔的第一阻抗并任选地从所述第一阻抗确定第一细胞指数，向至少一个含有测试细胞的孔中加入化合物以形成至少一种化合物，以及向至少另一个含有测试单元的井添加车辆控制以形成至少一个控制井，测量化合物井和控制井的第二阻抗，并且可选地从第二阻抗确定第二细胞指数，确定阻抗或细胞的变化指数为复合井和一个对照孔，比较了阻抗或细胞指数的变化如果比较表明化合物井和对照孔的阻抗变化或细胞指数之间存在显着差异，则鉴定化合物与GPCR或RTK相互作用。

7. 发明授权

US10215748B2 Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways 有权
公开(公告)号：US10215748B2
公开(公告)日：2019-02-26
申请号：US13403976
申请日：2012-02-23
申请人： Yama A. Abassi , Li Zhao , Ning Ke , Xiaobo Wang , Xiao Xu
发明人： Yama A. Abassi , Li Zhao , Ning Ke , Xiaobo Wang , Xiao Xu
IPC分类号： C40B30/04 , G01N33/50 , C12Q1/00 , G01N33/543
摘要： Use of impedance devices in methods of generating a time dependent cellular profiles (TCRP) for the modulation of oncogene addicted cells and comparing the impedance-based TCRP to controls or knowns to identify signature time dependent cellular profiles.

8. 发明申请

US20110300569A1 DATA ANALYSIS OF IMPEDANCE-BASED CARDIOMYOCYTE-BEATING SIGNALS AS DETECTED ON REAL-TIME CELL ANALYSIS (RTCA) CARDIO INSTRUMENTS 有权
标题翻译：基于实时细胞分析（RTCA）心脏仪器检测的基于阻抗的心脏纤维化信号的数据分析
公开(公告)号：US20110300569A1
公开(公告)日：2011-12-08
申请号：US13109809
申请日：2011-05-17
申请人： Nan Li , Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
发明人： Nan Li , Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： C12Q1/02
CPC分类号： G01N33/48728 , G01N33/4836 , G01N33/5008 , G01N33/5014 , G01N33/5061 , G01N33/5073 , G01N2800/326 , G01N2800/52
摘要： A method of determining one or more beating parameters for use in cardiomyocyte beating analysis including: providing a cell analysis device including wells, each well including a sensor capable of monitoring beating of cardiomyoctes in millisecond time resolution; adding cardiomyocytes to the wells; monitoring the beating of the cardiomyocytes in millisecond time resolution to obtain a plurality of beating measurements; and calculating one or more beating parameters from the plurality of beating measurements.
摘要翻译：一种确定用于心肌细胞搏动分析的一个或多个跳动参数的方法，包括：提供包括孔的细胞分析装置，每个孔包括能以毫秒时间分辨率监测心脏跳动的传感器; 向心脏中加入心肌细胞; 以毫秒时间分辨率监测心肌细胞的搏动以获得多次打浆测量; 以及从所述多次跳动测量中计算一个或多个跳动参数。

9. 发明授权

US09709548B2 Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution 有权
公开(公告)号：US09709548B2
公开(公告)日：2017-07-18
申请号：US12435569
申请日：2009-05-05
申请人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
发明人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： C40B30/06 , G01N7/00 , C12Q1/00 , G01N33/483
CPC分类号： G01N33/4836
摘要： Systems and methods for improved monitoring of excitation-contraction coupling and excitable cells are provided, which provide millisecond time resolution. The system is capable of continuously monitoring excitation-contraction coupling in a relatively high-throughput manner. The system includes a device for monitoring cell-substrate impedance, an impedance analyzer capable of impedance measurements at millisecond time resolution, electronic circuitry that can engage the device and selectively connect two or more electrode arrays of the device to the impedance analyzer and a software program that controls the electronic circuitry and records and analyzes data obtained from the impedance analyzer.

10. 发明授权

US08026080B2 Real time electronic cell sensing system and applications for cell-based assays 有权
标题翻译：实时电子传感系统和基于细胞的测定应用
公开(公告)号：US08026080B2
公开(公告)日：2011-09-27
申请号：US11725040
申请日：2007-03-15
申请人： Xiaobo Wang , Yama A. Abassi , Xiao Xu , Jiangbo Gan
发明人： Xiaobo Wang , Yama A. Abassi , Xiao Xu , Jiangbo Gan
IPC分类号： C12Q1/20 , C12Q1/08 , C12M1/34
CPC分类号： G01N33/6854 , C12M25/08 , C12M41/36 , G01N33/4836 , G01N33/5005 , G01N2500/10
摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.
摘要翻译：本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件用于控制设备站和阻抗分析仪。在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。在一些优选的方面，设计测定法以研究化合物对细胞的影响，例如细胞毒性测定。在其它优选方面，设计这些测定法来研究影响细胞对抗原的IgE介导的反应的化合物。

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