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1. 发明申请

US20120122165A1 RECOMBINANT MICROORGANISM AND METHOD FOR PRODUCING ALIPHATIC POLYESTER WITH THE USE OF THE SAME 审中-公开
标题翻译：重组微生物及其使用生产聚氨酯的方法
公开(公告)号：US20120122165A1
公开(公告)日：2012-05-17
申请号：US13387271
申请日：2010-07-27
申请人： Shusei Obata , Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
发明人： Shusei Obata , Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
IPC分类号： C12P7/62 , C12N1/21
CPC分类号： C12N9/13 , C12N9/1029 , C12P7/62
摘要： This invention provides a recombinant microorganism exhibiting excellent aliphatic polyester productivity and a method for producing an aliphatic polyester with the use of the recombinant microorganism. In this invention, the Megasphaera elsdenii-derived pct gene (and/or the Staphylococcus aureus-derived pct gene) and the Pseudomonas sp. 61-3 strain-derived PHA synthase gene (phaC2 gene) (and/or the Alcanivorax borkumensis SK2 strain-derived PHA synthase gene) are introduced into a host microorganism.
摘要翻译：本发明提供使用重组微生物显示优异脂肪族聚酯生产率的重组微生物和生产脂肪族聚酯的方法。在本发明中，来自大疱疹病毒的巨噬细胞源基因（和/或金黄色葡萄球菌来源的pct基因）和假单胞菌属（Pseudomonas sp。 61-3菌株衍生的PHA合酶基因（phaC2基因）（和/或阿尔卡氏菌属Bclumum SK2株衍生的PHA合酶基因）引入宿主微生物中。

2. 发明申请

US20110212497A1 METHOD FOR PRODUCTION OF POLYLACTATE USING RECOMBINANT MICROORGANISM 有权
标题翻译：使用重组微生物生产聚乙烯的方法
公开(公告)号：US20110212497A1
公开(公告)日：2011-09-01
申请号：US13126157
申请日：2009-10-27
申请人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
发明人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
IPC分类号： C12P7/62 , C12N1/00
CPC分类号： C12P7/62 , C12N9/1029
摘要： It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.The method is a method for producing a polylactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of one or several amino acid(s) other than the amino acids at positions 130, 325, 477, and 481 or by insertion of one or several amino acid residue(s); and (2) collecting the polylactate from the culture product obtained in step (1).
摘要翻译：本发明的目的是提供一种利用糖作为起始原料通过微生物发酵有效生产聚乳酸的方法。该方法是制备聚乳酸的方法，其包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸的反应的蛋白质的蛋白质和能够催化反应的蛋白质的重组微生物在含有碳源的培养基中由以下氨基酸序列（a）或（b）组成的聚羟基链烷酸酯合成物：（a）通过至少一个取代至少一个氨基酸序列的氨基酸序列，所述氨基酸序列来自SEQ ID NO：2所示的氨基酸序列的具有不同氨基酸的位置130,325,477和481处的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的一个或几个氨基酸的另外缺失或取代，或通过插入一个或几个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚乳酸。

3. 发明授权

US08748137B2 Method for production of polylactate using recombinant microorganism 有权
标题翻译：使用重组微生物生产聚乳酸的方法
公开(公告)号：US08748137B2
公开(公告)日：2014-06-10
申请号：US13126157
申请日：2009-10-27
申请人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
发明人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
IPC分类号： C12P7/62 , C12N9/00 , C12N9/10 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P7/62 , C12N9/1029
摘要： It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.The method is a method for producing a polylactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of one or several amino acid(s) other than the amino acids at positions 130, 325, 477, and 481 or by insertion of one or several amino acid residue(s); and (2) collecting the polylactate from the culture product obtained in step (1).
摘要翻译：本发明的目的是提供一种利用糖作为起始原料通过微生物发酵有效生产聚乳酸的方法。该方法是制备聚乳酸的方法，其包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸的反应的蛋白质的蛋白质和能够催化反应的蛋白质的重组微生物在含有碳源的培养基中由以下氨基酸序列（a）或（b）组成的聚羟基链烷酸酯合成物：（a）通过至少一个取代至少一个氨基酸序列的氨基酸序列，所述氨基酸序列来自SEQ ID NO：2所示的氨基酸序列的具有不同氨基酸的位置130,325,477和481处的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的一个或几个氨基酸的另外缺失或取代，或通过插入一个或几个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚乳酸。

4. 发明授权

US08980615B2 Recombinant microorganism and method for producing aliphatic polyester using the same 有权
标题翻译：重组微生物及其制备方法
公开(公告)号：US08980615B2
公开(公告)日：2015-03-17
申请号：US13636830
申请日：2011-03-24
申请人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
发明人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
IPC分类号： C12N1/20 , C12P7/62 , C12N9/10
CPC分类号： C12P7/625 , C12N9/1029 , C12N9/13 , C12Y203/01 , C12Y208/03001
摘要： Aliphatic polyester productivity is improved for production of aliphatic polyester using a recombinant microorganism. A recombinant microorganism prepared by introducing a gene encoding a protein having activity of converting lactic acid to lactic-acid CoA and a gene encoding a protein having activity of synthesizing polyhydroxyalkanoate using hydroxyacyl CoA as a substrate into a host microorganism is cultured and then aliphatic polyester is recovered from the medium.
摘要翻译：使用重组微生物生产脂肪族聚酯的脂肪族聚酯生产率得到改善。培养通过将编码具有将乳酸转化为乳酸CoA的蛋白质的蛋白质的基因和使用羟基酰基CoA作为底物合成聚羟基链烷酸酯的蛋白质的基因的基因导入宿主微生物而制备的重组微生物，然后将脂肪族聚酯从培养基中回收。

5. 发明申请

US20130045516A1 RECOMBINANT MICROORGANISM AND METHOD FOR PRODUCING ALIPHATIC POLYESTER USING THE SAME 有权
标题翻译：重组微生物及使用其生产聚氨酯的方法
公开(公告)号：US20130045516A1
公开(公告)日：2013-02-21
申请号：US13636830
申请日：2011-03-24
申请人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
发明人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
IPC分类号： C12P7/62 , C12N1/21
CPC分类号： C12P7/625 , C12N9/1029 , C12N9/13 , C12Y203/01 , C12Y208/03001
摘要： Aliphatic polyester productivity is improved for production of aliphatic polyester using a recombinant microorganism. A recombinant microorganism prepared by introducing a gene encoding a protein having activity of converting lactic acid to lactic-acid CoA and a gene encoding a protein having activity of synthesizing polyhydroxyalkanoate using hydroxyacyl CoA as a substrate into a host microorganism is cultured and then aliphatic polyester is recovered from the medium.
摘要翻译：使用重组微生物生产脂肪族聚酯的脂肪族聚酯生产率得到改善。培养通过将编码具有将乳酸转化为乳酸CoA的蛋白质的蛋白质的基因和使用羟基酰基CoA作为底物合成聚羟基链烷酸酯的蛋白质的基因的基因导入宿主微生物而制备的重组微生物，然后将脂肪族聚酯从培养基中回收。

6. 发明授权

US08563281B2 Method for production of polyester copolymer using recombinant microorganism 有权
标题翻译：使用重组微生物生产聚酯共聚物的方法
公开(公告)号：US08563281B2
公开(公告)日：2013-10-22
申请号：US12989333
申请日：2009-04-23
申请人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsunori Kohda , Katsuhiro Ohno , Takashi Shimamura
发明人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsunori Kohda , Katsuhiro Ohno , Takashi Shimamura
IPC分类号： C12P7/62 , C12N1/20 , C12N1/00
CPC分类号： C12P7/62
摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate via microbial fermentation with the use of a sugar as a starting material. The method may comprise culturing a recombinant microorganism expressing: a protein capable of catalyzing the transfer of CoA to propionic acid and/or lactate; a protein capable of catalyzing the formation of acetoacetyl-CoA from two acetyl-CoA molecules; a protein capable of catalyzing acetoacetyl-CoA reduction; and a protein capable of catalyzing polyhydroxyalkanoate synthesis. According to the production method, a polyester copolymer consisting of 3-hydroxybutyrate and lactate can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.
摘要翻译：本发明的目的是提供一种通过使用糖作为原料通过微生物发酵有效地制备由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的方法。该方法可以包括培养表达：能够催化CoA转移到丙酸和/或乳酸盐的蛋白质的重组微生物; 能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的蛋白质; 能够催化乙酰乙酰辅酶A还原的蛋白质; 和能够催化聚羟基链烷酸酯合成的蛋白质。根据该制造方法，可以使用廉价的碳源作为起始材料有效地制造由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

7. 发明申请

US20110104768A1 METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM 有权
标题翻译：使用重组微生物生产聚酯共聚物的方法
公开(公告)号：US20110104768A1
公开(公告)日：2011-05-05
申请号：US12989333
申请日：2009-04-23
申请人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsuhiro Kohda , Katsuhiro Ohno , Takashi Shimamura
发明人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsuhiro Kohda , Katsuhiro Ohno , Takashi Shimamura
IPC分类号： C12P7/62 , C12N1/00 , C12N1/21
CPC分类号： C12P7/62
摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material.The following is provided: a method for producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate, a protein capable of catalyzing a reaction of forming acetoacetyl-CoA from two acetyl-CoA molecules, a protein capable of catalyzing a reaction of acetoacetyl-CoA reduction, and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of at least one amino acid other than the amino acids at positions 130, 325, 477, and 481 or by insertion of at least one amino acid residue; and (2) collecting the polyester copolymer from the culture product obtained in step (1). According to the production method of the present invention, a polyester copolymer consisting of 3HB and LA can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.
摘要翻译：本发明的目的是提供一种利用糖作为原料，通过微生物发酵有效地制备由3HB和LA组成的聚酯共聚物的方法。提供以下：由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的制造方法，其特征在于，包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸盐，能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的反应的蛋白质，能够催化乙酰乙酰辅酶A还原反应的蛋白质，以及能够催化由下列氨基组成的聚羟基链烷酸酯合成反应的蛋白质含有碳源的培养基中的酸序列（a）或（b）：（a）从SEQ ID NO：2所示的氨基酸序列衍生的氨基酸序列，通过将位置130的至少一个氨基酸置换，325,477和481具有不同的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的至少一个氨基酸的另外缺失或取代或通过插入至少一个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚酯共聚物。根据本发明的制造方法，可以使用廉价的碳源作为原料，有效地制造由3HB和LA组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

8. 发明申请

US20130157327A1 MUTANT POLYHYDROXYALKANOIC ACID SYNTHASE GENE AND METHOD FOR PRODUCING ALIPHATIC POLYESTER USING THE SAME 有权
标题翻译：突变性聚羟基烷基酸合成酶基因及其制备方法
公开(公告)号：US20130157327A1
公开(公告)日：2013-06-20
申请号：US13809536
申请日：2010-07-14
申请人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito
发明人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito
IPC分类号： C12N9/10
CPC分类号： C12P7/625 , C12N9/1029
摘要： A substitution mutation that improves polymerization activity of a polyhydroxyalkanoic acid synthase is identified. At least 1 amino acid residue selected from the group consisting of a histidine residue at position 17, a proline residue at position 71, a valine residue at position 131, a methionine residue at position 205, a leucine residue at position 230, and a proline residue at position 239 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis is subjected to substitution mutation with another amino acid.
摘要翻译：鉴定出提高聚羟基链烷酸合酶聚合活性的取代突变。至少1个氨基酸残基选自17位组氨酸残基，71位脯氨酸残基，131位缬氨酸残基，205位甲硫氨酸残基，230位亮氨酸残基和脯氨酸来自阿尔卡氏菌的聚羟基链烷酸合酶的位置239处的残基用另一种氨基酸进行取代突变。

9. 发明授权

US08802402B2 Mutant polyhydroxyalkanoic acid synthase gene and method for producing aliphatic polyester using the same 有权
标题翻译：突变型聚羟基链烷酸合酶基因及使用其的脂肪族聚酯的制造方法
公开(公告)号：US08802402B2
公开(公告)日：2014-08-12
申请号：US13809536
申请日：2010-07-14
申请人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito
发明人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito
IPC分类号： C12P7/62
CPC分类号： C12P7/625 , C12N9/1029
摘要： A substitution mutation that improves polymerization activity of a polyhydroxyalkanoic acid synthase is identified. At least 1 amino acid residue selected from the group consisting of a histidine residue at position 17, a proline residue at position 71, a valine residue at position 131, a methionine residue at position 205, a leucine residue at position 230, and a proline residue at position 239 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis is subjected to substitution mutation with another amino acid.
摘要翻译：鉴定出提高聚羟基链烷酸合酶聚合活性的取代突变。至少1个氨基酸残基选自17位组氨酸残基，71位脯氨酸残基，131位缬氨酸残基，205位甲硫氨酸残基，230位亮氨酸残基和脯氨酸来自阿尔卡氏菌的聚羟基链烷酸合酶的位置239处的残基用另一种氨基酸进行取代突变。

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