专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5188935A Reagent system and method for identification, enumeration and examination of classes and subclasses of blood leukocytes 失效
标题翻译：用于鉴定，计数和检查血白细胞类和亚类的试剂系统和方法
公开(公告)号：US5188935A
公开(公告)日：1993-02-23
申请号：US611381
申请日：1990-11-13
申请人： Robert C. Leif , Stephen L. Ledis , Robert I. Feinberg
发明人： Robert C. Leif , Stephen L. Ledis , Robert I. Feinberg
IPC分类号： G01N33/50 , G01N33/569
CPC分类号： G01N33/50 , G01N33/56972 , Y10T436/107497
摘要： Specified classes and subclasses of leukocyte blood cells are identified by immunohematology procedures, based on utilization of antigenic determinants on the cell surface, their reactivity with antibodies which fluoresce under known circumstances, and identified by utilization of principles of flow cytometry or morphology.This invention particularly concerns improvements in the lysing and fixing method used prior to detection and identifying of the cells. In this method, a whole blood sample first is incubated with a reagent including antibodies to the cell subclass to be identified, the antibodies having directly or indirectly made fluorescently responsive to a particular light (e.g. argon ion laser). The red blood cells then are lysed with a reagent containing saponin. Next follows a leukocyte fixing treatment, preferably using a cross-linking dialdehyde composition, such as glyoxal or glutaraldehyde.Details are given as to time, temperature, concentration, and the use of additives and stains, as well as the use of particular monoclonal antibodies in the preferred procedure.
摘要翻译：基于细胞表面上的抗原决定簇的利用，其与已知情况下发荧光的抗体的反应性以及通过使用流式细胞术或形态学原理鉴定的免疫血液学方法来鉴定白细胞血细胞的指定类别和亚类。本发明特别涉及在检测和鉴定细胞之前使用的裂解和固定方法的改进。在该方法中，首先将全血样品与包括要鉴别的细胞亚类的抗体的试剂一起温育，所述抗体直接或间接地对特定的光进行荧光反应（例如氩离子激光）。然后用含有皂苷的试剂将红细胞裂解。接下来进行白细胞固定处理，优选使用交联二醛组合物，例如乙二醛或戊二醛。给出关于时间，温度，浓度以及添加剂和污渍的使用以及在优选方法中使用特定单克隆抗体的细节。

2. 发明授权

US5373093A Macrocyclic complexes of yttrium, the lanthanides and the actinides having peripheral coupling functionalities 失效
标题翻译：钇，镧系元素和具有外围偶联官能团的锕系元素的大环络合物
公开(公告)号：US5373093A
公开(公告)日：1994-12-13
申请号：US669833
申请日：1991-03-15
申请人： Lidia M. Vallarino , Robert C. Leif
发明人： Lidia M. Vallarino , Robert C. Leif
IPC分类号： G01N33/533 , A61K31/555 , A61K33/24 , A61K49/00 , A61K49/06 , A61K51/00 , A61P35/00 , A61P37/00 , C07D471/18 , C07D487/18 , C07D491/18 , C07D495/18 , C07D513/18 , C07F5/00 , G01N33/58 , A61K39/395 , C07D221/00
CPC分类号： C07D471/18 , A61K49/06 , C07D491/18 , C07D495/18 , G01N33/582 , G01N2458/40
摘要： Functionalized water soluble macrocyclic complexes of lanthanide, actinide and yttrium ions were obtained by metal templated, Schiff-base, cyclic condensation of: (1) a functionalized 1,2-diaminoethane and a dicarbonyl compound selected from the group consisting of 2,6-dicarbonylpyridine, 2,6-diformylpyridine, 2,5-dicarbonylfuran, 2,5-diformylfuran, 2,5-dicarbonylthiophene and 2,5-diformylthiophene; or (2) 1,2-diaminoethane and a ring-substituted heterocyclic dicarbonyl compound selected from a group consisting of substituted 2,6-dicarbonylpyridine, substituted 2,6-diformylpyridine, substituted 2,5-dicarbonylfuran, substituted 2,5-diformylfuran; substituted 2,5-dicarbonyl thiophene, and substituted 2,5-diformylthiophene. Coordination complexes thus formed are kinetically stable in dilute aqueous solution. They are further reacted, or coupled, through a substituent on the 1,2-diaminoethane or on the pyridine, furan, or thiophene moieties, to one of the following: proteinaceous materials, polysaccharides, other biologically compatible macromolecules or bridging molecules which, can be further reacted or coupled to the above mentioned substrates. These macrocyclic complexes are suitable in the preparation of reporter molecules and for magnetic resonance, radiation imaging and radiation therapy.
摘要翻译：通过金属模板化，席夫碱，环状缩合得到镧系元素，锕系元素和钇离子的官能化水溶性大环络合物：（1）官能化的1,2-二氨基乙烷和选自2,6-二甲基苯基的二羰基化合物，二羰基吡啶，2,6-二甲酰基吡啶，2,5-二羰基呋喃，2,5-二甲基呋喃，2,5-二羰基噻吩和2,5-二甲基噻吩; 或（2）1,2-二氨基乙烷和环取代的杂环二羰基化合物，其选自取代的2,6-二羰基吡啶，取代的2,6-二甲酰基吡啶，取代的2,5-二羰基呋喃，取代的2,5-二甲基呋喃 ; 取代的2,5-二羰基噻吩和取代的2,5-二甲酰基噻吩。如此形成的配位络合物在稀水溶液中动力学稳定。它们进一步通过1,2-二氨基乙烷或吡啶，呋喃或噻吩部分上的取代基反应或偶联到以下之一：蛋白质材料，多糖，其它生物相容性大分子或桥接分子，其可以进一步反应或耦合到上述基底。这些大环配合物适用于报道分子的制备和磁共振，放射成像和放射治疗。

3. 发明授权

US5798827A Apparatus and method for determination of individual red blood cell shape 失效
标题翻译：用于确定个体红细胞形状的装置和方法
公开(公告)号：US5798827A
公开(公告)日：1998-08-25
申请号：US753585
申请日：1996-11-26
申请人： Robert S. Frank , James L. Wyatt , Weiyan Gong , Carlos M. Rodriguez , Robert C. Leif
发明人： Robert S. Frank , James L. Wyatt , Weiyan Gong , Carlos M. Rodriguez , Robert C. Leif
IPC分类号： G01N33/49 , G01N15/00 , G01N15/10 , G01N15/14 , G01N21/47
CPC分类号： G01N15/1459 , G01N2015/0073 , G01N2015/1037 , G01N2015/1495 , G01N2015/1497 , G01N2021/4716
摘要： The present invention provides an apparatus and method to determine individual red blood cell shape within a flow cytometric aperture. Cell shape is determined by measuring the asymmetry of a light scatter pattern produced by laser illumination of the cell within the aperture by light scatter detectors which collect light from specific angular regions of light scatter, and analyzing the asymmetry of the light scatter patters to determine the individual red blood cell shape. Embodiments of the invention further provide for the determination of red blood cell shape by measuring the asymmetry of a light scatter pattern and measuring electrical resistance of the red blood cell.
摘要翻译：本发明提供了一种确定流式细胞术孔内单个红细胞形状的装置和方法。单元形状通过测量由光散射检测器产生的光散射图案的不对称性，光散射检测器从光散射的特定角度区域收集光，并分析光散射图案的不对称性，以确定个体红细胞形状。本发明的实施例进一步提供了通过测量光散射图案的不对称性并测量红细胞的电阻来确定红细胞形状。

4. 发明申请

US20160290998A1 REAGENT SYSTEM AND METHOD FOR MODIFYING THE LUMINESCENCE OF LANTHANIDE (III) MACROCYCLIC COMPLEXES 审中-公开
标题翻译：试剂系统及其修饰方法（III）大分子复合物
公开(公告)号：US20160290998A1
公开(公告)日：2016-10-06
申请号：US14526248
申请日：2014-10-28
申请人： Robert C. Leif , Sean Yang , Lidia Vallarino
发明人： Robert C. Leif , Sean Yang , Lidia Vallarino
IPC分类号： G01N33/542 , G01N21/64 , C12Q1/68 , G01N33/58
CPC分类号： G01N33/542 , C12Q1/6818 , G01N21/6428 , G01N21/6458 , G01N33/582 , G01N2021/6441 , G01N2458/40
摘要： A spectrofluorimetrically detectable luminescent composition consists essentially of at least one energy transfer acceptor lanthanide(III) complex having an emission spectrum maximum in the range from 300 to 2000 nanometers and a luminescence-enhancing amount of at least one energy transfer donor selected from the group consisting of a fluorophore, a lumiphore, an organic compound, a salt of an organic ion, a metal ion, a metal ion complex, or a combination thereof. Such energy transfer donor enhances the luminescence of at least one energy transfer acceptor lanthanide(III) complex, with the conditions that the emission spectrum of any energy transfer donor differs from that of its energy transfer acceptor lanthanide(III) complex; and such energy transfer donor can be dissolved to form a unitary solution in a solvent having an evaporation rate at least as great as that of water.
摘要翻译：分光荧光检测发光组合物基本上由至少一种发射光谱最大值在300至2000纳米范围内的能量转移受体镧系元素（III）络合物组成，以及至少一种能量转移供体的增亮量，该能量转移受体选自的荧光团，发光体，有机化合物，有机离子的盐，金属离子，金属离子络合物或其组合。这种能量转移供体增强至少一种能量转移受体镧系元素（III）络合物的发光，条件是任何能量转移供体的发射光谱与其能量转移受体镧系元素（III）络合物的发射光谱不同; 并且这样的能量转移供体可以溶解在具有与水的蒸发速率至少一样大的溶剂中的单一溶液。

5. 发明授权

US06750005B2 Reagent system and method for increasing the luminescence of lanthanide(III) macrocyclic complexes 失效
标题翻译：用于增加镧系元素（III）大环络合物发光的试剂体系和方法
公开(公告)号：US06750005B2
公开(公告)日：2004-06-15
申请号：US10010597
申请日：2001-12-06
申请人： Robert C. Leif , Lidia Vallarino
发明人： Robert C. Leif , Lidia Vallarino
IPC分类号： C12Q100
CPC分类号： C07D471/18 , G01N33/582 , Y10S435/96 , Y10S435/968
摘要： Disclosed are a spectrofluorimetrically detectable luminescent composition and processes for enhancing the luminescence of one or more lanthanide-containing macrocycles. The luminescent composition comprises a micelle-producing amount of at least one surfactant, at least one energy transfer acceptor lanthanide element macrocycle compound having an emission spectrum peak in the range from 500 to 950 nanometers, and a luminescence-enhancing amount of at least one energy transfer donor compound of yttrium or a 3-valent lanthanide element having atomic number 59-71, provided that the lanthanide element of said macrocycle compound and the lanthanide element of said energy transfer donor compound are not identical. The addition of gadolinium(III) in the presence of other solutes to both the prototype and the di-functionalized europium, samarium, and terbium macrocyclic complexes, which were taught in our U.S. Pat. Nos. 5,373,093 and 5,696,240, enhances their luminescence. Similar enhancements of luminescence also results for the mono-functionalized europium, samarium, and terbium macrocyclic complexes, which were taught in our U.S. Pat. No. 5,696,240. The enhanced luminescence afforded by the composition enables the detection and/or quantitation of many analytes in low concentrations without the use of expensive, complicated time-gated detection systems.
摘要翻译：公开了分光荧光测定的发光组合物和用于增强一种或多种含镧系元素的大环化合物的发光的方法。发光组合物包含胶束产生量的至少一种表面活性剂，至少一种能量转移受体镧系元素大环化合物，其发射光谱峰值在500至950纳米的范围内，以及至少一种能量的发光增强量转移钇的供体化合物或原子序数为59-71的3价镧系元素，条件是所述大环化合物的镧系元素和所述能量转移给体化合物的镧系元素不相同。在其他溶质存在下，将钆（III）添加到原型和二官能化的铕，钐和铽大环络合物中，这在我们的美国专利No. 第5,373,093和第5,696,240号，增强了它们的发光。对于单官能化的铕，钐和铽大环复合物，发光的类似增强也得到了结果，这在我们的美国专利中被教导。第5,696,240号。由组合物提供的增强的发光使得能够以低浓度检测和/或定量许多分析物，而不需要使用昂贵，复杂的时间门控检测系统。

6. 发明授权

US08916692B2 Reagent system and method for modifying the luminescence of lanthanide(III) macrocyclic complexes 有权
标题翻译：用于改变镧系元素（III）大环络合物发光的试剂体系和方法
公开(公告)号：US08916692B2
公开(公告)日：2014-12-23
申请号：US10578355
申请日：2004-11-08
申请人： Robert C. Leif , Sean Yang , Lidia Vallarino
发明人： Robert C. Leif , Sean Yang , Lidia Vallarino
IPC分类号： C07F5/00 , G01N21/64 , G01N33/533 , A61B5/00
CPC分类号： G01N33/533 , G01N21/6428 , G01N2021/6441
摘要： Disclosed is a spectrofluorimetrically detectable luminescent composition consisting essentially of at least one energy transfer acceptor lanthanide(III) complex having an emission spectrum maximum in the range from 300 to 2000 nanometers and a luminescence-enhancing amount of at least one energy transfer donor selected from the group consisting of a fluorophore, a lumiphore, an organic compound, a salt of an organic ion, a metal ion, a metal ion complex, or a combination thereof. Such energy transfer donor enhances the luminescence of at least one energy transfer acceptor lanthanide(III) complex, with the conditions that the emission spectrum of any energy transfer donor differs from that of its energy transfer acceptor lanthanide(III) complex; and such energy transfer donor can be dissolved to form a unitary solution in a solvent having an evaporation rate at least as great as that of water.
摘要翻译：公开了一种分光荧光测定的发光组合物，其基本上由至少一种发射光谱最大值在300至2000纳米的能量转移受体镧系元素（III）络合物组成，并且增亮量的至少一种能量转移给体选自由荧光团，荧光体，有机化合物，有机离子的盐，金属离子，金属离子络合物或其组合组成的组。这种能量转移供体增强至少一种能量转移受体镧系元素（III）络合物的发光，条件是任何能量转移供体的发射光谱与其能量转移受体镧系元素（III）络合物的发射光谱不同; 并且这样的能量转移供体可以溶解在具有与水的蒸发速率至少一样大的溶剂中的单一溶液。

7. 发明授权

US08048659B1 Conjugated polymer tag complexes 失效
标题翻译：共轭聚合物标签复合物
公开(公告)号：US08048659B1
公开(公告)日：2011-11-01
申请号：US10110114
申请日：2000-10-07
申请人： Robert C. Leif , Lidia Vallarino
发明人： Robert C. Leif , Lidia Vallarino
IPC分类号： G01N33/53
CPC分类号： G01N33/582 , C08F289/00 , C08F290/14 , C08F291/00 , C08L51/08 , C12Q1/37
摘要： A tagged water-soluble polymer linked to a solid support is provided and includes a solid support optionally including a spacer sequence including one or more spacer monomer units attached to the solid support; and a water-soluble polymer covalently linked to the solid support or to the spacer sequence, the water-soluble polymer including a first cleavage segment including at least one monomer unit including a selectively cleavable link to the solid support or to the spacer sequence, where cleavage of the selectively cleavable link separates the water-soluble polymer from the solid support, a second segment including at least one monomer unit selected from a monomer unit linked to a reactive functionality able to be covalently coupled to a tag or linked to a tag, a third segment including one or more monomer units linked to a reactive functionality that can form a covalent bond with an analyte-binding species or an analyte, and optionally one or more spacer monomer units provided adjacent to any segment and/or between any two segments and/or within any one or more of the second segment and the third segment.
摘要翻译：提供与固体支持物连接的标记的水溶性聚合物，并且包括固体支持物，任选地包括间隔物序列，其包括连接到固体支持物上的一个或多个间隔单体单元; 和与固体支持体或间隔物序列共价连接的水溶性聚合物，所述水溶性聚合物包括第一切割区段，所述第一切割区段包含至少一个单体单元，所述单体单元包括与所述固体支持物或所述间隔物序列的选择性可切割的连接，其中可选择性切割链节的切割将水溶性聚合物与固体支持物分开，第二部分包括至少一个单体单元，所述单体单元选自与能够共价耦合到标签或连接到标签的反应性官能团的单体单元，第三片段，其包含连接到可与分析物结合物质或分析物形成共价键的反应性官能团的一个或多个单体单元，以及任选地与任何片段相邻和/或任意两个片段之间设置的一个或多个间隔单体单元和/或在第二段和第三段中的任何一个或多个内。

8. 发明授权

US5696240A Macrocyclic complexes of yttrium, the lanthanides and the actinides having peripheral coupling functionalities 失效
标题翻译：钇，镧系元素和具有外围偶联官能团的锕系元素的大环络合物
公开(公告)号：US5696240A
公开(公告)日：1997-12-09
申请号：US351827
申请日：1994-12-08
申请人： Lidia M. Vallarino , Robert C. Leif
发明人： Lidia M. Vallarino , Robert C. Leif
IPC分类号： C07F5/00 , G01N33/58 , A61K49/00 , A61B5/055 , A61K39/395
CPC分类号： C07F5/003 , G01N33/582 , G01N2458/40
摘要： Symmetrically di-functionalized water soluble macrocyclic complexes of lanthanide, actinide and yttrium ions were obtained by metal templated, Schiff-base, cyclic condensation of: (1) a functionalized 1,2-diaminoethane and a dicarbonyl compound selected from the group consisting of 2,6-dicarbonylpyridine, 2,6-diformylpyridine, 2,5-dicarbonylfuran, 2,5-diformylfuran, 2,5-dicarbonyl-thiophene and 2,5-diformylthiophene; or (2) 1,2-diaminoethane and a ring-substituted heterocyclic dicarbonyl compound selected from a group consisting of substituted 2,6-dicarbonylpyridine, substituted 2,6-diformylpyridine, substituted 2,5-dicarbonylfuran, substituted 2,5-diformylfuran; substituted 2,5-dicarbonyl thiophene, and substituted 2,5-diformylthiophene. Asymmetrically functionalized water soluble macrocyclic complexes of the lanthanide, actinide and yttrium ions were obtained by metal templated, Schiff-base, cyclic condensation of appropriately substituted diamine and dicarbonyl precursors, with such precursors contributing two heteroaromatic moieties (pyridine, furan, thiophene, or a combination thereof) to the resulting macrocyclic structure. The coordination complexes thus formed are kinetically stable in dilute aqueous solution. They are further reacted, or coupled, through a substituent on the 1,2-diaminoethane or on the pyridine, furan, or thiophene moieties, to one of the following: proteinaceous materials, polysaccharides, polynucleotides, other biologically compatible macromolecules or bridging molecules which, can be further reacted or coupled to the above mentioned substrates. These macrocyclic complexes are suitable in the preparation of reporter molecules and for magnetic resonance, radiation imaging and radiation therapy.
摘要翻译：通过金属模板化，席夫碱，环状缩合获得镧系元素，锕系元素和钇离子的对称二官能化的水溶性大环络合物：（1）官能化的1,2-二氨基乙烷和二羰基化合物，其选自2 ，6-二羰基吡啶，2,6-二甲酰基吡啶，2,5-二羰基呋喃，2,5-二甲基呋喃，2,5-二羰基噻吩和2,5-二甲基噻吩; 或（2）1,2-二氨基乙烷和环取代的杂环二羰基化合物，其选自取代的2,6-二羰基吡啶，取代的2,6-二甲酰基吡啶，取代的2,5-二羰基呋喃，取代的2,5-二甲基呋喃 ; 取代的2,5-二羰基噻吩和取代的2,5-二甲酰基噻吩。通过金属模板化，席夫碱，适当取代的二胺和二羰基前体的环缩合获得镧系元素，锕系元素和钇离子的不对称官能化的水溶性大环络合物，其中这些前体贡献两个杂芳族部分（吡啶，呋喃，噻吩或其组合）与所得的大环结构。如此形成的配位络合物在稀水溶液中动力学稳定。它们进一步通过1,2-二氨基乙烷或吡啶，呋喃或噻吩部分上的取代基反应或偶联到以下之一：蛋白质材料，多糖，多核苷酸，其它生物相容性大分子或桥接分子，其中，可进一步反应或与上述底物偶联。这些大环配合物适用于报道分子的制备和磁共振，放射成像和放射治疗。

9. 发明授权

US06340744B1 Reagent system and method for increasing the luminescence of lanthanide(III) macrocyclic complexes 失效
标题翻译：用于增加镧系元素（III）大环络合物发光的试剂体系和方法
公开(公告)号：US06340744B1
公开(公告)日：2002-01-22
申请号：US09484670
申请日：2000-01-18
申请人： Robert C. Leif , Lidia Vallarino
发明人： Robert C. Leif , Lidia Vallarino
IPC分类号： A61K4900
CPC分类号： C07D471/18 , G01N33/582 , Y10S435/96 , Y10S435/968
摘要： Disclosed are a spectrofluorimetrically detectable luminescent composition and processes for enhancing the luminescence of one or more lanthanide-containing macrocycles. The luminescent composition comprises a micelle-producing amount of at least one surfactant, at least one energy transfer acceptor lanthanide element macrocycle compound having an emission spectrum peak in the range from 500 to 950 nanometers, and a luminescence-enhancing amount of at least one energy transfer donor compound of yttrium or a 3-valent lanthanide element having atomic number 59-71, provided that the lanthanide element of said macrocycle compound and the lanthanide element of said energy transfer donor compound are not identical. The addition of gadolinium(III) in the presence of other solutes to both the prototype and the difunctionalized europium, samarium, and terbium macrocyclic complexes, which were taught in our U.S. Pat. Nos. 5,373,093 and 5,696,240, enhances their luminescence. Similar enhancements of luminescence also results for the mono-functionalized europium, samarium, and terbium macrocyclic complexes, which were taught in our U.S. Pat. No. 5,696,240. The enhanced luminescence afforded by the composition enables the detection and/or quantitation of many analytes in low concentrations without the use of expensive, complicated time-gated detection systems.
摘要翻译：公开了分光荧光测定的发光组合物和用于增强一种或多种含镧系元素的大环化合物的发光的方法。发光组合物包含胶束产生量的至少一种表面活性剂，至少一种能量转移受体镧系元素大环化合物，其发射光谱峰值在500至950纳米的范围内，以及至少一种能量的发光增强量转移钇的供体化合物或原子序数为59-71的3价镧系元素，条件是所述大环化合物的镧系元素和所述能量转移给体化合物的镧系元素不相同。在其他溶质存在下，将钆（III）添加到原型和双官能化铕，钐和铽大环络合物中，这在我们的美国专利中已被教导。第5,373,093和第5,696,240号，增强了它们的发光。对于单官能化的铕，钐和铽大环复合物，发光的类似增强也得到了结果，这在我们的美国专利中被教导。第5,696,240号。由组合物提供的增强的发光使得能够以低浓度检测和/或定量许多分析物，而不需要使用昂贵，复杂的时间门控检测系统。

10. 发明授权

US4351611A Monitoring of a detection zone utilizing zero order radiation from a concave reflecting grating 失效
标题翻译：使用来自凹反射光栅的零级辐射来监测检测区域
公开(公告)号：US4351611A
公开(公告)日：1982-09-28
申请号：US189505
申请日：1980-09-22
申请人： Robert C. Leif
发明人： Robert C. Leif
IPC分类号： G01N15/14 , G01J3/18
CPC分类号： G01N15/1434 , G01N15/1459
摘要： Disclosed is an optical flow system wherein individual particles, which are sequentially suspended in a fluid flow, are irradiated at a detection zone with an excitation source to generate radiation signals, which in turn are dispersed by a concave reflection grating for subsequent detection and measurement. The radiation signals which are not dispersed from the grating but are reflected from the grating, are further reflected by a conjugate mirror to form a projected image of the detection zone suitable for viewing with a screen or eyepiece.
摘要翻译：公开了一种光流系统，其中顺序悬浮在流体流中的单个颗粒在具有激发源的检测区域处照射以产生辐射信号，辐射信号又由凹面反射光栅分散以用于随后的检测和测量。不从光栅分散但被光栅反射的辐射信号被共轭反射镜进一步反射，以形成适合于用屏幕或目镜观看的检测区域的投影图像。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式