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1. 发明申请

US20120100534A1 EFFICIENT BASE DETERMINATION IN SEQUENCING REACTIONS 有权
标题翻译：顺序反应中有效的碱基测定
公开(公告)号：US20120100534A1
公开(公告)日：2012-04-26
申请号：US12329365
申请日：2008-12-05
申请人： Radoje Drmanac , Andrew Sparks , Fredrik Dahl , Matthew J. Callow , Clifford Reid
发明人： Radoje Drmanac , Andrew Sparks , Fredrik Dahl , Matthew J. Callow , Clifford Reid
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6855 , C12Q2531/125 , C12Q2521/313 , C12Q2521/125 , C12Q2537/163 , C12Q2525/191 , C12Q2521/531 , C12Q2521/501 , C12Q2525/307 , C12Q2525/151
摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译：本发明涉及用于核酸鉴定和检测的组合物和方法。本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。

2. 发明授权

US08298768B2 Efficient shotgun sequencing methods 有权
标题翻译：高效霰弹枪测序方法
公开(公告)号：US08298768B2
公开(公告)日：2012-10-30
申请号：US12325922
申请日：2008-12-01
申请人： Radoje Drmanac , Clifford Reid
发明人： Radoje Drmanac , Clifford Reid
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2549/119 , C12Q2537/149 , C12Q2565/137
摘要： Methods are provided for efficient shotgun sequencing to allow efficient selection and sequencing of nucleic acids of interest contained in a library. The nucleic acids of interest can be defined any time before or after preparation of the library. One example of nucleic acids of interest is missing or low confidence genome sequences resulting from an initial sequencing procedure. Other nucleic acids of interest include subsets of genomic DNA, RNA or cDNAs (exons, genes, gene sets, transciptomes). By designing an efficient (simple to implement, speedy, high specificity, low cost) selection procedure, a more complete sequence is achieved with less effort than by using highly redundant shotgun sequencing in an initial sequencing procedure.
摘要翻译：提供了有效的霰弹枪测序的方法，以允许文库中包含的感兴趣的核酸的有效选择和测序。感兴趣的核酸可以在文库制备之前或之后的任何时间进行定义。感兴趣的核酸的一个实例是由初始测序程序产生的缺失或低置信基因组序列。感兴趣的其他核酸包括基因组DNA，RNA或cDNAs（外显子，基因，基因组，转录本）的子集。通过设计一种高效（简单易于实施，快速，高特异性，低成本）选择程序，通过在初始测序程序中使用高度冗余的霰弹枪测序，以更少的努力实现更完整的顺序。

3. 发明申请

US20090176234A1 Efficient base determination in sequencing reactions 有权
标题翻译：测序反应中有效的碱基测定
公开(公告)号：US20090176234A1
公开(公告)日：2009-07-09
申请号：US12335188
申请日：2008-12-15
申请人： Radoje Drmanac , Andrew Sparks , Fredrik Dahl , Matthew J. Callow , Clifford Reid
发明人： Radoje Drmanac , Andrew Sparks , Fredrik Dahl , Matthew J. Callow , Clifford Reid
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6855 , C12Q2531/125 , C12Q2521/313 , C12Q2521/125 , C12Q2537/163 , C12Q2525/191 , C12Q2521/531 , C12Q2521/501 , C12Q2525/307 , C12Q2525/151
摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译：本发明涉及用于核酸鉴定和检测的组合物和方法。本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。

4. 发明申请

US20090318304A1 Efficient Shotgun Sequencing Methods 有权
标题翻译：高效霰弹枪测序方法
公开(公告)号：US20090318304A1
公开(公告)日：2009-12-24
申请号：US12325922
申请日：2008-12-01
申请人： Radoje Drmanac , Clifford Reid
发明人： Radoje Drmanac , Clifford Reid
IPC分类号： C40B30/04
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2549/119 , C12Q2537/149 , C12Q2565/137
摘要： Methods are provided for efficient shotgun sequencing to allow efficient selection and sequencing of nucleic acids of interest contained in a library. The nucleic acids of interest can be defined any time before or after preparation of the library. One example of nucleic acids of interest is missing or low confidence genome sequences resulting from an initial sequencing procedure. Other nucleic acids of interest include subsets of genomic DNA, RNA or cDNAs (exons, genes, gene sets, transciptomes). By designing an efficient (simple to implement, speedy, high specificity, low cost) selection procedure, a more complete sequence is achieved with less effort than by using highly redundant shotgun sequencing in an initial sequencing procedure
摘要翻译：提供了有效的霰弹枪测序的方法，以允许文库中包含的感兴趣的核酸的有效选择和测序。感兴趣的核酸可以在文库制备之前或之后的任何时间进行定义。感兴趣的核酸的一个实例是由初始测序程序产生的缺失或低置信基因组序列。感兴趣的其他核酸包括基因组DNA，RNA或cDNAs（外显子，基因，基因组，转录本）的子集。通过设计一种高效（简单实施，快速，高特异性，低成本）选择程序，通过在初始测序程序中使用高度冗余的霰弹枪测序，以更少的努力实现更完整的顺序

5. 发明授权

US09551026B2 Method for nucleic acid detection using voltage enhancement 有权
标题翻译：使用电压增强的核酸检测方法
公开(公告)号：US09551026B2
公开(公告)日：2017-01-24
申请号：US13337968
申请日：2011-12-27
申请人： Andres Fernandez , Bryan Staker , Radoje Drmanac
发明人： Andres Fernandez , Bryan Staker , Radoje Drmanac
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6825 , C12Q2523/307 , C12Q2565/501 , C12Q2565/607
摘要： Methods are provided for carrying out DNA sequencing on a device having upper and lower conductive layers separated by an insulative layer. Holes in the upper conductive layer create discrete attachment sites for DNA fragments. Voltage is applied to the surface to control affinity between the attachment sites and the DNA fragments, and to compact the DNA fragments for discrete optical detection.
摘要翻译：提供了用于在具有由绝缘层隔开的上导电层和下导电层的器件上进行DNA测序的方法。上导电层中的孔为DNA片段产生离散的附着位点。将电压施加到表面以控制附着位点和DNA片段之间的亲和力，并压缩DNA片段以进行离散的光学检测。

6. 发明授权

US09023769B2 cDNA library for nucleic acid sequencing 有权
标题翻译：用于核酸测序的cDNA文库
公开(公告)号：US09023769B2
公开(公告)日：2015-05-05
申请号：US12956802
申请日：2010-11-30
申请人： Radoje Drmanac , Fredrik Dahl , Evan Hurowitz , Fredrie Dahl
发明人： Radoje Drmanac , Fredrik Dahl , Evan Hurowitz , Fredrie Dahl
IPC分类号： C40B50/06 , C12Q1/68 , C12N15/10 , C40B20/00
CPC分类号： C12Q1/6869 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , C12N15/10 , C12Q1/6853 , C40B20/00
摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译：本发明涉及用于核酸鉴定和检测的组合物和方法。本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。

7. 发明授权

US08617811B2 Methods and compositions for efficient base calling in sequencing reactions 有权
公开(公告)号：US08617811B2
公开(公告)日：2013-12-31
申请号：US12361507
申请日：2009-01-28
申请人： Radoje Drmanac
发明人： Radoje Drmanac
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/34 , C12M3/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6874 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6876 , C12Q2565/102 , C12Q2565/1025 , C12Q2565/518
摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences. In particular, the present invention provides methods and compositions for improving the efficiency of sequencing reactions by using fewer labels to distinguish between nucleotides and by detecting nucleotides at multiple detection positions in a target sequence.

8. 发明授权

US08592150B2 Methods and compositions for long fragment read sequencing 有权
标题翻译：用于长片段读取测序的方法和组合物
公开(公告)号：US08592150B2
公开(公告)日：2013-11-26
申请号：US12816365
申请日：2010-06-15
申请人： Radoje Drmanac , Brock A. Peters , Andrei Alexeev , Peter Hong
发明人： Radoje Drmanac , Brock A. Peters , Andrei Alexeev , Peter Hong
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： B01J19/0046 , B01J2219/00722 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q2525/101 , C12Q2521/101
摘要： The present invention is directed to methods and compositions for long fragment read sequencing. The present invention encompasses methods and compositions for preparing long fragments of genomic DNA, for processing genomic DNA for long fragment read sequencing methods, as well as software and algorithms for processing and analyzing sequence data.
摘要翻译：本发明涉及用于长片段读取测序的方法和组合物。本发明包括用于制备基因组DNA长片段的方法和组合物，用于处理长片段读取测序方法的基因组DNA，以及用于处理和分析序列数据的软件和算法。

9. 发明授权

US08440397B2 High throughput genome sequencing on DNA arrays 有权
公开(公告)号：US08440397B2
公开(公告)日：2013-05-14
申请号：US11981761
申请日：2007-10-31
申请人： Radoje Drmanac , Matthew J. Callow , Snezana Drmanac
发明人： Radoje Drmanac , Matthew J. Callow , Snezana Drmanac
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/36
CPC分类号： C12Q1/6874 , C12N15/64 , C12N15/66 , Y10T436/143333 , C12Q2521/313 , C12Q2525/191 , C12Q2525/151 , C12Q2525/131 , C12Q2565/518 , C12Q2533/107 , C12Q2531/125
摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.

10. 发明申请

US20130059740A1 Sequencing Small Amounts of Complex Nucleic Acids 审中-公开
标题翻译：测序小量的复杂核酸
公开(公告)号：US20130059740A1
公开(公告)日：2013-03-07
申请号：US13448279
申请日：2012-04-16
申请人： Radoje Drmanac , Brock A. Peters , Bahram Ghaffarzadeh Kermani
发明人： Radoje Drmanac , Brock A. Peters , Bahram Ghaffarzadeh Kermani
IPC分类号： C40B20/00 , C40B20/04
CPC分类号： G16B30/00
摘要： The present invention provides methods and compositions for sequencing small amounts of complex nucleic acids such as human genomes and for analyzing the resulting sequence information in order to reduce sequencing errors and perform haplotype phasing, for example.
摘要翻译：本发明提供了用于测序少量复合核酸如人类基因组的方法和组合物，并且用于分析所得序列信息以减少测序误差并进行例如单倍型定相。

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