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1. 发明授权

US07776521B1 Coronavirus isolated from humans 有权
标题翻译：从人类分离的冠状病毒
公开(公告)号：US07776521B1
公开(公告)日：2010-08-17
申请号：US11748359
申请日：2007-05-14
申请人： Paul A. Rota , Larry J. Anderson , William J. Bellini , Michael D. Bowen , Cara Carthel Burns , Raymond Campagnoli , Qi Chen , James A. Comer , Byron T. Cook , Shannon L. Emery , Dean D. Erdman , Cynthia S. Goldsmith , Jeanette Guarner , Charles D. Humphrey , Joseph P. Icenogle , Thomas G. Ksiazek , Richard F. Meyer , Stephan S. Monroe , William Allan Nix , M. Steven Oberste , Christopher D. Paddock , Teresa C. T. Peret , Pierre E. Rollin , Mark A. Pallansch , Anthony Sanchez , Wun-Ju Shieh , Suxiang Tong , Sherif R. Zaki
发明人： Paul A. Rota , Larry J. Anderson , William J. Bellini , Michael D. Bowen , Cara Carthel Burns , Raymond Campagnoli , Qi Chen , James A. Comer , Byron T. Cook , Shannon L. Emery , Dean D. Erdman , Cynthia S. Goldsmith , Jeanette Guarner , Charles D. Humphrey , Joseph P. Icenogle , Thomas G. Ksiazek , Richard F. Meyer , Stephan S. Monroe , William Allan Nix , M. Steven Oberste , Christopher D. Paddock , Teresa C. T. Peret , Pierre E. Rollin , Mark A. Pallansch , Anthony Sanchez , Wun-Ju Shieh , Suxiang Tong , Sherif R. Zaki
IPC分类号： C12Q1/70 , C07H21/00 , C12N15/50
CPC分类号： C07K14/005 , A61K39/12 , A61K39/215 , C12N7/00 , C12N2770/20021 , C12N2770/20022 , C12N2770/20034
摘要： Disclosed herein is a newly isolated human coronavirus (SARS-CoV), the causative agent of severe acute respiratory syndrome (SARS). Also provided are the nucleic acid sequence of the SARS-CoV genome and the amino acid sequences of the SARS-CoV open reading frames, as well as methods of using these molecules to detect a SARS-CoV and detect infections therewith. Immune stimulatory compositions are also provided, along with methods of their use.
摘要翻译：本文公开了新分离的人冠状病毒（SARS-CoV），严重急性呼吸综合征（SARS）的致病因子。还提供了SARS-CoV基因组的核酸序列和SARS-CoV开放阅读框的氨基酸序列，以及使用这些分子检测SARS-CoV并检测其感染的方法。还提供免疫刺激组合物及其使用方法。

2. 发明授权

US07220852B1 Coronavirus isolated from humans 有权
标题翻译：从人类分离的冠状病毒
公开(公告)号：US07220852B1
公开(公告)日：2007-05-22
申请号：US10822904
申请日：2004-04-12
申请人： Paul A. Rota , Larry J. Anderson , William J. Bellini , Cara Carthel Burns , Raymond Campagnoli , Qi Chen , James A. Comer , Shannon L. Emery , Dean D. Erdman , Cynthia S. Goldsmith , Charles D. Humphrey , Joseph P. Icenogle , Thomas G. Ksiazek , Stephan S. Monroe , William Allan Nix , M. Steven Oberste , Teresa C. T. Peret , Pierre E. Rollin , Mark A. Pallansch , Anthony Sanchez , Suxiang Tong , Sherif R. Zaki
发明人： Paul A. Rota , Larry J. Anderson , William J. Bellini , Cara Carthel Burns , Raymond Campagnoli , Qi Chen , James A. Comer , Shannon L. Emery , Dean D. Erdman , Cynthia S. Goldsmith , Charles D. Humphrey , Joseph P. Icenogle , Thomas G. Ksiazek , Stephan S. Monroe , William Allan Nix , M. Steven Oberste , Teresa C. T. Peret , Pierre E. Rollin , Mark A. Pallansch , Anthony Sanchez , Suxiang Tong , Sherif R. Zaki
IPC分类号： C12N15/50 , C12N7/00
CPC分类号： C07K14/005 , A61K39/12 , A61K39/215 , C12N7/00 , C12N2770/20021 , C12N2770/20022 , C12N2770/20034
摘要： Disclosed herein is a newly isolated human coronavirus (SARS-CoV), the causative agent of severe acute respiratory syndrome (SARS). Also provided are the nucleic acid sequence of the SARS-CoV genome and the amino acid sequences of the SARS-CoV open reading frames, as well as methods of using these molecules to detect a SARS-CoV and detect infections therewith. Immune stimulatory compositions are also provided, along with methods of their use.
摘要翻译：本文公开了新分离的人冠状病毒（SARS-CoV），严重急性呼吸综合征（SARS）的致病因子。还提供了SARS-CoV基因组的核酸序列和SARS-CoV开放阅读框的氨基酸序列，以及使用这些分子检测SARS-CoV并检测其感染的方法。还提供免疫刺激组合物及其使用方法。

3. 发明授权

US08048630B2 Methods and agents for detecting Parechovirus 有权
标题翻译：检测鱼腥草病的方法和试剂
公开(公告)号：US08048630B2
公开(公告)日：2011-11-01
申请号：US12299097
申请日：2006-05-01
申请人： William Allan Nix , M. Steven Oberste
发明人： William Allan Nix , M. Steven Oberste
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/701 , C12N2770/32511
摘要： The present disclosure provides methods that permit detection of all known species of Parechovirus, including Human parechovirus and Ljungan virus (LV). In particular examples the method includes amplifying at least a portion of the 5′NTR of parechovirus nucleic acid molecules obtained from a sample, and detecting the resulting amplicons, but does not require culturing of the virus. The present disclosure also provides methods for determining which particular species or serotype of parechovirus is present in a biological sample. Also provided are oligonucleotide primers and probes that can be used in the disclosed methods.
摘要翻译：本公开提供了允许检测所有已知种类的Parechovirus，包括人类parechovirus和Ljungan病毒（LV）的方法。在具体实例中，该方法包括扩增从样品获得的鹦鹉病毒核酸分子的5NTR的至少一部分，并检测所得扩增子，但不需要培养病毒。本公开还提供了用于确定生物样品中存在有哪些特定种类或血清型的parechovirus的方法。还提供了可用于所公开的方法中的寡核苷酸引物和探针。

4. 发明授权

US07247457B2 Detection and identification of enteroviruses by semi-nested amplification of the enterovirus VP1 protein 有权
标题翻译：通过肠病毒VP1蛋白的半嵌套扩增检测和鉴定肠道病毒
公开(公告)号：US07247457B2
公开(公告)日：2007-07-24
申请号：US11115860
申请日：2005-04-26
申请人： William Allan Nix , M. Steven Oberste
发明人： William Allan Nix , M. Steven Oberste
IPC分类号： C12P19/34 , C12Q1/70
CPC分类号： C12Q1/701
摘要： Disclosed are methods of using enterovirus-specific primers for the detection and identification of enterovirus infection. Also provided are isolated nucleic acid molecules and kits useful for detection and diagnostic testing of enterovirus infection in a subject.
摘要翻译：公开了使用肠病毒特异性引物来检测和鉴定肠道病毒感染的方法。还提供了分离的核酸分子和试剂盒，其可用于受试者中肠道病毒感染的检测和诊断测试。

5. 发明授权

US07714122B2 Nucleic acid molecules and kits including VP1 and VP3 nucleic acid molecules, useful for detecting and identifying enteroviruses 有权
标题翻译：核酸分子和试剂盒，包括VP1和VP3核酸分子，可用于检测和鉴定肠道病毒
公开(公告)号：US07714122B2
公开(公告)日：2010-05-11
申请号：US11777916
申请日：2007-07-13
申请人： William Allan Nix , M. Steven Oberste
发明人： William Allan Nix , M. Steven Oberste
IPC分类号： C07H21/04
CPC分类号： C12Q1/701
摘要： Disclosed are methods of using enterovirus-specific primers for the detection and identification of enterovirus infection. Also provided are isolated nucleic acid molecules and kits useful for detection and diagnostic testing of enterovirus infection in a subject.
摘要翻译：公开了使用肠病毒特异性引物来检测和鉴定肠道病毒感染的方法。还提供了分离的核酸分子和试剂盒，其可用于受试者中肠道病毒感染的检测和诊断测试。

6. 发明申请

US20100035230A1 METHODS AND AGENTS FOR DETECTING PARECHOVIRUS 有权
标题翻译：检测PARECHOVIRUS的方法和试剂
公开(公告)号：US20100035230A1
公开(公告)日：2010-02-11
申请号：US12299097
申请日：2006-05-01
申请人： William Allan Nix , M. Steven Oberste
发明人： William Allan Nix , M. Steven Oberste
IPC分类号： C12Q1/70 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/701 , C12N2770/32511
摘要： The present disclosure provides methods that permit detection of all known species of Parechovirus, including Human parechovirus and Ljungan virus (LV). In particular examples the method includes amplifying at least a portion of the 5′NTR of parechovirus nucleic acid molecules obtained from a sample, and detecting the resulting amplicons, but does not require culturing of the virus. The present disclosure also provides methods for determining which particular species or serotype of parechovirus is present in a biological sample. Also provided are oligonucleotide primers and probes that can be used in the disclosed methods.
摘要翻译：本公开提供了允许检测所有已知种类的Parechovirus，包括人类parechovirus和Ljungan病毒（LV）的方法。在具体实例中，该方法包括扩增从样品获得的鹦鹉病毒核酸分子的5NTR的至少一部分，并检测所得扩增子，但不需要培养病毒。本公开还提供了用于确定生物样品中存在有哪些特定种类或血清型的parechovirus的方法。还提供了可用于所公开的方法中的寡核苷酸引物和探针。

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