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1. 发明申请

US20090186804A1 ANTICOAGULANT AND COMPOSITION FOR PREVENTING THROMBUS CONTAINING POLY-GAMMA-GLUTAMIC ACID 审中-公开
标题翻译：用于预防含有多糖 - 谷氨酸的含沙门氏菌的抗凝血剂和组合物
公开(公告)号：US20090186804A1
公开(公告)日：2009-07-23
申请号：US12159561
申请日：2006-12-22
申请人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Haryoung Poo , Tae-Woo Kim
发明人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Haryoung Poo , Tae-Woo Kim
IPC分类号： A61K38/00 , A61P7/02
CPC分类号： A61K31/74 , A23L33/175 , A61K9/0095
摘要： The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient. The inventive PGA is a water-soluble, anionic, biodegradable and edible amino acid polymer material, which has an anticoagulant effect of preventing thrombi from being accumulated in blood vessels, shows an excellent sustained-release effect and is harmless to the human body. Thus, it is useful as a high-value-added anticoagulant and a food or beverage composition for preventing thrombus formation.
摘要翻译：本发明涉及含有聚-γ-谷氨酸（PGA）作为活性成分的用于预防血栓形成的抗凝血剂和组合物。本发明的PGA是一种水溶性，阴离子，可生物降解和可食用的氨基酸聚合物材料，其具有防止血栓在血管中积聚的抗凝作用，显示出优异的持续释放作用并对人体无害。因此，作为高附加值抗凝剂和用于预防血栓形成的食品或饮料组合物是有用的。

2. 发明授权

US08618057B2 Anticoagulant and composition for preventing thrombus containing poly-gamma-glutamic acid 失效
标题翻译：用于预防含有聚-γ-谷氨酸的血栓的抗凝血剂和组合物
公开(公告)号：US08618057B2
公开(公告)日：2013-12-31
申请号：US13444324
申请日：2012-04-11
申请人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Haryoung Poo , Tae-Woo Kim
发明人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Haryoung Poo , Tae-Woo Kim
IPC分类号： A61K38/02
CPC分类号： A61K31/74 , A23L33/175 , A61K9/0095
摘要： The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient. The inventive PGA is a water-soluble, anionic, biodegradable and edible amino acid polymer material, which has an anticoagulant effect of preventing thrombi from being accumulated in blood vessels, shows an excellent sustained-release effect and is harmless to the human body. Thus, it is useful as a high-value-added anticoagulant and a food or beverage composition for preventing thrombus formation.
摘要翻译：本发明涉及含有聚-γ-谷氨酸（PGA）作为活性成分的用于预防血栓形成的抗凝血剂和组合物。本发明的PGA是一种水溶性，阴离子，可生物降解和可食用的氨基酸聚合物材料，其具有防止血栓在血管中积聚的抗凝作用，显示出优异的持续释放作用并对人体无害。因此，作为高附加值抗凝剂和用于预防血栓形成的食品或饮料组合物是有用的。

3. 发明申请

US20060199185A1 Plasmid having a function of t-vector and expression vector, and expression of the target gene using the same 审中-公开
标题翻译：具有t载体和表达载体的功能的质粒以及使用其的靶基因的表达
公开(公告)号：US20060199185A1
公开(公告)日：2006-09-07
申请号：US10564880
申请日：2003-12-31
申请人： Young Song , Haryoung Poo , Moon-Hee Sung , Seung-Pyo Hong , Yoon Ho Choi , Kwang Kim , Il Han Lee , Je Hyun Park
发明人： Young Song , Haryoung Poo , Moon-Hee Sung , Seung-Pyo Hong , Yoon Ho Choi , Kwang Kim , Il Han Lee , Je Hyun Park
IPC分类号： C40B40/02 , C40B40/08 , C12Q1/68 , C12N15/74
CPC分类号： C12N15/64
摘要： The present invention relates to a plasmid (pHCE-FOREX) functioning as both a T-vector and an expression vector, which is produced by imparting a T-vector function to an HCE promoter derived from a constitutive high-level expression vector and can express a target protein in a simple and rapid manner. Also, the present invention relates to an expression vector having the target gene inserted into the plasmid, and the expression of the target gene using the same. The plasmid of the present invention can be converted into a vector that expresses the target protein by one-step T-vector cloning in a simple and rapid manner. The plasmid converted into the expression vector does not require a re-transformation step and allows the high-level expression of the target protein only by the culturing of transformed E. coli without the addition of an expensive inducer. Thus, according to the present invention, expression plasmids for large amounts of target genes can be produced at the same time, so that the present invention will be very efficient in establishing expression systems for certain genomes and gene groups.
摘要翻译：本发明涉及用作T载体和表达载体两者的质粒（pHCE-FOREX），其通过赋予来自组成型高水平表达载体的HCE启动子产生T载体功能而产生，并可表达目标蛋白以简单快速的方式。此外，本发明涉及将靶基因插入质粒的表达载体，以及使用该表达载体的靶基因的表达。本发明的质粒可通过简单快速的一步T载体克隆转化为表达靶蛋白的载体。转化成表达载体的质粒不需要再转化步骤，并且仅通过培养转化的大肠杆菌而不添加昂贵的诱导物才允许靶蛋白的高水平表达。因此，根据本发明，可以同时生产大量靶基因的表达质粒，从而本发明在建立某些基因组和基因组的表达系统方面将非常有效。

4. 发明授权

US08236940B2 Constitutive strong promoter and use thereof 有权
标题翻译：组成型强启动子及其用途
公开(公告)号：US08236940B2
公开(公告)日：2012-08-07
申请号：US12532605
申请日：2008-03-21
申请人： Moon Hee Sung , Chul Joong Kim , Seung-Pyo Hong , Haryoung Poo , Il Han Lee , Ji Yeon Kim , Kwang Kim
发明人： Moon Hee Sung , Chul Joong Kim , Seung-Pyo Hong , Haryoung Poo , Il Han Lee , Ji Yeon Kim , Kwang Kim
IPC分类号： C07H21/04 , C12N15/63 , C12N1/21 , C12P21/00
CPC分类号： C12N15/746 , C12N9/1276
摘要： Disclosed herein are an aldolase gene promoter and the use thereof, more particularly, disclosed are a promoter of aldolase gene derived from Lactobacillus casei, having a base sequence of SEQ ID NO: 1, an expression vector containing said promoter, and a recombinant microorganism transformed with said expression vector. The recombinant microorganism transformed with the expression vector containing the disclosed promoter can effectively express a target protein on the surface thereof, and thus will be useful as vaccine vehicles and the like.
摘要翻译：本文公开了醛缩酶基因启动子及其用途，更具体地，公开了源自干酪乳杆菌的醛缩酶基因的启动子，其具有SEQ ID NO：1的碱基序列，含有所述启动子的表达载体和转化的重组微生物与所述表达载体。用含有公开的启动子的表达载体转化的重组微生物可以在其表面上有效地表达靶蛋白质，因此可用作疫苗载体等。

5. 发明申请

US20100196956A1 NOVEL CONSTITUTIVE STRONG PROMOTER AND USE THEREOF 有权
标题翻译：新宪法强力促进者及其使用
公开(公告)号：US20100196956A1
公开(公告)日：2010-08-05
申请号：US12532605
申请日：2008-03-21
申请人： Moon Hee Sung , Chul Joong Kim , Seung-Pyo Hong , Haryoung Poo , Il Han Lee , Ji Yeon Kim , Kwang Kim
发明人： Moon Hee Sung , Chul Joong Kim , Seung-Pyo Hong , Haryoung Poo , Il Han Lee , Ji Yeon Kim , Kwang Kim
IPC分类号： C12P21/06 , C07H21/04 , C12N15/63 , C12N1/00 , C12N1/21
CPC分类号： C12N15/746 , C12N9/1276
摘要： Disclosed herein are an aldolase gene promoter and the use thereof, more particularly, disclosed are a promoter of aldolase gene derived from Lactobacillus casei, having a base sequence of SEQ ID NO: 1, an expression vector containing said promoter, and a recombinant microorganism transformed with said expression vector. The recombinant microorganism transformed with the expression vector containing the disclosed promoter can effectively express a target protein on the surface thereof, and thus will be useful as vaccine vehicles and the like.
摘要翻译：本文公开了醛缩酶基因启动子及其用途，更具体地，公开了源自干酪乳杆菌的醛缩酶基因的启动子，其具有SEQ ID NO：1的碱基序列，含有所述启动子的表达载体和转化的重组微生物与所述表达载体。用含有公开的启动子的表达载体转化的重组微生物可以在其表面上有效地表达靶蛋白质，因此可用作疫苗载体等。

6. 发明申请

US20060127447A1 Poly-gamma-glutamate having ultra high molecular weight and method for using the same 审中-公开
标题翻译：具有超高分子量的聚-γ-谷氨酸盐及其使用方法
公开(公告)号：US20060127447A1
公开(公告)日：2006-06-15
申请号：US10520557
申请日：2003-07-10
申请人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Kwang Seok Kim , Jae Jun Song , Eu Gene Rha , Kwang Kim , Kenji Soda , Makoto Ashiuchi , Ha Ryoung Poo , Seung Lee
发明人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Kwang Seok Kim , Jae Jun Song , Eu Gene Rha , Kwang Kim , Kenji Soda , Makoto Ashiuchi , Ha Ryoung Poo , Seung Lee
IPC分类号： A61K38/16 , A61K47/00 , C07K14/32 , C12N1/21 , C12P21/06
CPC分类号： A61K9/0014 , A61K8/88 , A61K9/0095 , A61K47/42 , A61Q19/00 , C08H1/00 , C12P21/02
摘要： The present invention relates to a poly-gamma-glutamate (PGA) having an ultra-high molecular weight greater than 5,000 kDa. The ultra-high molecular weight PGA according to the present invention has a mean molecular weight greater than 13,000 kDa, and more than 95% of its molecules have a molecular weight ranging from 3,000 to 15,000 kDa. Also, it can be produced by the culturing of Bacillus subtilis var. chungkookjang. The ultra-high molecular weight PGA according to the present invention shows very excellent moisture-absorbing, moisture-retaining, sustained release, mineral solubility, and water-absorbing properties, and thus, can be used as a new and high value-added material in various applications.
摘要翻译：本发明涉及超高分子量大于5,000kDa的聚-γ-谷氨酸（PGA）。根据本发明的超高分子量PGA的平均分子量大于13000kDa，其分子的95％以上具有3,000至15,000kDa的分子量。此外，它可以通过培养枯草芽孢杆菌变种产生。 ung ko ang。根据本发明的超高分子量PGA显示出非常优异的吸湿性，保湿性，缓释性，矿物溶解性和吸水性，因此可用作新的高附加值材料在各种应用中。

7. 发明授权

US07501262B2 Promoters and gene expression method by using the promoters 有权
标题翻译：启动子和使用启动子的基因表达方法
公开(公告)号：US07501262B2
公开(公告)日：2009-03-10
申请号：US11599475
申请日：2006-11-15
申请人： Moon-Hee Sung , Seung-Goo Lee , Seung-Pyo Hong , Hwa-Jung Seo
发明人： Moon-Hee Sung , Seung-Goo Lee , Seung-Pyo Hong , Hwa-Jung Seo
IPC分类号： C12P21/00 , C12N1/21 , C12N15/70 , C12N15/74 , C07H21/04
CPC分类号： C07K14/32 , C12N15/70 , C12N15/75
摘要： To provide a promoter, a recombinant DNA, a gene expression vector, an expression vector, and a transformant, which are capable of expressing a gene without inducing gene expression with an inducer; and a method for producing a protein and a kit therefor, which can be operated easily and performed inexpensively by convenient and inexpensive steps. An isolated DNA having the nucleotide sequence of SEQ ID NO: 1 or 2 of Sequence Listing or a fragment thereof, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; an isolated DNA having a nucleotide sequence of a nucleic acid capable of hybridizing to the above DNA, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; a recombinant DNA comprising the above DNA and a foreign gene, wherein the foreign gene is operably located; a gene expression vector, at least comprising the above DNA; an expression vector comprising the recombinant DNA; a transformant having the above recombinant DNA, or the above expression vector; a method for producing a protein, characterized by culturing the above transformant, and collecting a protein from the resulting culture; and a kit for producing a protein, at least comprising the above DNA, or the above gene expression vector.
摘要翻译：提供能够表达基因而不诱导基因表达的启动子，重组DNA，基因表达载体，表达载体和转化体; 以及用于制备蛋白质及其试剂盒的方法，其可以容易地操作并且通过方便和便宜的步骤廉价地进行。具有序列表的SEQ ID NO：1或2的核苷酸序列或其片段的分离的DNA，其中所述分离的DNA在大肠杆菌或属于芽孢杆菌属的细菌中显示组成型启动子活性; 具有能够与上述DNA杂交的核酸的核苷酸序列的分离的DNA，其中所述分离的DNA在大肠杆菌或属于芽孢杆菌属的细菌中显示出组成型启动子活性; 包含上述DNA和外来基因的重组DNA，其中所述外源基因可操作地定位; 至少包含上述DNA的基因表达载体; 包含重组DNA的表达载体; 具有上述重组DNA的转化体或上述表达载体; 一种生产蛋白质的方法，其特征在于培养上述转化体，并从得到的培养物中收集蛋白质; 以及用于生产至少包含上述DNA或上述基因表达载体的蛋白质的试剂盒。

8. 发明申请

US20070253935A1 SURFACE EXPRESSION METHOD OF PEPTIDES P5 AND ANAL3 USING THE GENE ENCODING POLY-GAMMA-GLUTAMATE SYNTHETASE 有权
标题翻译：使用基因编码多糖 - 谷氨酸合成酶的肽P5和ANAL3的表面表达方法
公开(公告)号：US20070253935A1
公开(公告)日：2007-11-01
申请号：US11622158
申请日：2007-01-11
申请人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Kyung-Soo Hahim , Dong-Gun Lee , Yoon Park , Chul-Joong Kim , Ha-Ryoung Poo
发明人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Kyung-Soo Hahim , Dong-Gun Lee , Yoon Park , Chul-Joong Kim , Ha-Ryoung Poo
IPC分类号： A61K39/00 , C12N1/21 , C12N15/00
CPC分类号： C07K14/32
摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal3 35.
摘要翻译：本发明涉及使用载体表达在微生物表面上表现出具有两亲性的肽抗生素P 5 3和Anal 3 35的各种抗细菌，抗真菌和抗癌活性61,63,65,67,69,71的方法，含有源自芽孢杆菌属的外膜蛋白基因（pgsBCA）。菌株并参与了聚-γ-谷氨酸的合成。此外，本发明涉及具有表面表达的肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。因此，本发明的方法具有非常高的工业实用性。此外，除了P 5 3和Anal 3 35之外，本发明可以应用于其他肽抗生素。

9. 发明授权

US07553636B2 Surface expression vectors having pgsBCA the gene coding poly-gamma-glutamate synthetase, and a method for expression of target protein at the surface of microorganism using the vector 有权
标题翻译：具有编码聚-γ-谷氨酸合成酶的基因的pgsBCA的表达表达载体，以及使用载体在微生物表面表达靶蛋白的方法
公开(公告)号：US07553636B2
公开(公告)日：2009-06-30
申请号：US10484605
申请日：2002-08-09
申请人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Chul-Joong Kim , Kenji Soda , Makoto Ashiuchi
发明人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Chul-Joong Kim , Kenji Soda , Makoto Ashiuchi
IPC分类号： C12N15/09 , C12N1/21 , C12N15/63
CPC分类号： C12N9/1051 , C07K2319/02 , C12N9/1288 , C12Y204/01133 , C12Y207/08005
摘要： The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.
摘要翻译：本发明涉及具有pgsBCA的表达表达载体，编码聚-γ-谷氨酸合成酶的基因和使用该载体在微生物表面表达靶蛋白的方法。插入外来基因的载体转化微生物，使外源蛋白稳定表达在微生物表面。

10. 发明授权

US07323328B2 Obligately symbiotic thermophile Symbiobacterium toebii SC-1 producing thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase 有权
标题翻译：共生共生嗜热菌共培养杆菌SC-1产生热稳定性L-酪氨酸苯酚裂解酶和L-色氨酸吲哚裂解酶
公开(公告)号：US07323328B2
公开(公告)日：2008-01-29
申请号：US11186559
申请日：2005-07-21
申请人： Moon-Hee Sung , Seung-Goo Lee , Sung-Keun Rhee , Seung-Pyo Hong , Dae-Joong Kang , Su-Mi Kim , Chul-Joong Kim , Jin-Woo Bae , Che Ok Jeon , Joong-Jae Kim , Kwang Kim , Jae Jun Song
发明人： Moon-Hee Sung , Seung-Goo Lee , Sung-Keun Rhee , Seung-Pyo Hong , Dae-Joong Kang , Su-Mi Kim , Chul-Joong Kim , Jin-Woo Bae , Che Ok Jeon , Joong-Jae Kim , Kwang Kim , Jae Jun Song
IPC分类号： C12N1/20 , C12N9/88 , C12P39/00
CPC分类号： C12R1/01 , C12N9/88 , C12P13/227
摘要： The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1 (Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebii SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.
摘要翻译：本发明涉及一种新型的共生共生嗜热ile ile i i i i i i i i））i i i i i i i i i i i i i i i i i。。。。。。。。。。。。。。。。。。。。本发明还涉及使用硝酸盐呼吸的共生杆菌SC-1的纯培养方法，使用硝酸盐呼吸显示低生长产量的共生杆菌SC-1及其相对共生菌的生长方法，以及相对共生菌株的筛选方法的来自环境的共表达杆菌SC-1使用特异性抗体。因此，在本发明中由共生杆菌（Toibii）SC-1产生的热稳定性L-酪氨酸苯酚裂解酶和L-色氨酸吲哚裂解酶可以在酶生物转化过程中提供更稳定的催化剂，其中酶促产生有价值的羟基 - 氨基酸3,4-脱羟基 - L-苯丙氨酸（L-DOPA）和L-色氨酸。

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