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1. 发明授权

US08927236B2 Process for producing poly-γ-glutamic acid having high optical purity 有权
标题翻译：具有高光学纯度的聚-γ-谷氨酸的制造方法
公开(公告)号：US08927236B2
公开(公告)日：2015-01-06
申请号：US12223067
申请日：2007-01-31
申请人： Makoto Ashiuchi , Kazuki Shimizu
发明人： Makoto Ashiuchi , Kazuki Shimizu
IPC分类号： C12P13/14 , C12R1/11 , C12P21/02
CPC分类号： C12R1/11 , C12P13/14 , C12P21/02
摘要： It is an objective of the present invention to provide a process for efficiently producing poly-γ-glutamic acid having a high L-glutamic acid content and excellent quality. The process for producing poly-γ-glutamic acid according to the present invention is characterized in using a bacterium belonging to species Bacillus megaterium.
摘要翻译：本发明的目的是提供一种高效制备L-谷氨酸含量高，品质优良的聚-γ-谷氨酸的方法。根据本发明的制备聚-γ-谷氨酸的方法的特征在于使用属于芽孢杆菌（Bacillus megaterium）的细菌。

2. 发明申请

US20090203790A1 GAMMA-L-PGA PRODUCING MICROORGANISM, METHOD OF PRODUCING GAMMA-L-PGA USING THE MICROORGANISM, CROSSLINKED SUBSTANCE PRODUCED USING THE MICROORGANISM, AND EXTERNAL DERMAL AGENT PRODUCED USING THE MICROORGANISM 有权
标题翻译：生产微生物的GAMMA-L-PGA，使用微生物生产GAMMA-L-PGA的方法，使用微生物生产的交联物质和使用微生物生产的外源性物质
公开(公告)号：US20090203790A1
公开(公告)日：2009-08-13
申请号：US12301411
申请日：2007-03-12
申请人： Shuhei Yamamoto , Masaru Kitagawa , Michiko Suzuki , Atsushi Sogabe , Makoto Ashiuchi
发明人： Shuhei Yamamoto , Masaru Kitagawa , Michiko Suzuki , Atsushi Sogabe , Makoto Ashiuchi
IPC分类号： A61K31/195 , C12N1/21 , C12P13/14 , C12Q1/04
CPC分类号： A61Q19/00 , A61K8/88 , C08G69/10 , C08L77/04 , C08L2205/02 , C12P21/02 , C08L2666/20
摘要： There are provided a microorganism characterized by producing poly-γ-L-glutamate with a molecular weight of 1,300,000 or greater and uniform optical purity under liquid culture conditions, a method of screening for the microorganism, a method of producing poly-γ-L-glutamate having large molecular weight by using the microorganism, and poly-γ-L-glutamate having an average molecular weight of 1,300,000 or greater. In addition, usages of these are provided.
摘要翻译：提供了一种微生物，其特征在于在液体培养条件下生产分子量为130万或更大，光学纯度均匀的聚-γ-L-谷氨酸，筛选微生物的方法，生产聚-γ- 通过使用微生物具有大分子量的谷氨酸盐，以及平均分子量为130万以上的聚-γ-L-谷氨酸。此外，还提供了这些用途。

3. 发明授权

US07109008B2 L-glutamate oxidase 有权
标题翻译： L-谷氨酸氧化酶
公开(公告)号：US07109008B2
公开(公告)日：2006-09-19
申请号：US10257398
申请日：2001-04-19
申请人： Kenji Inagaki , Jiro Arima , Makoto Ashiuchi , Toshiharu Yagi , Hitoshi Kusakabe
发明人： Kenji Inagaki , Jiro Arima , Makoto Ashiuchi , Toshiharu Yagi , Hitoshi Kusakabe
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12Q1/68 , C12Q1/34 , C12P21/04 , C07H21/04
CPC分类号： C12Y104/03011 , C12N9/0022
摘要： The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique. The novel L-glutamate oxidase has the following physicochemical properties: (A) action: catalyzing the following reaction: L-glutamic acid+O2+H2O→α-ketoglutaric acid+H2O2+NH3; (B) substrate specificity: being specific to L-glutamic acid; (C) molecular weight and subunit structure: molecular weight as determined through SDS-polyacrylamide gel electrophoresis of 70,000±6,000, molecular weight as determined through gel filtration of 140,000±10,000, and being a dimer formed of the same subunits having a molecular weight of 70,000±6,000; (D) optimum pH: around pH 6.0 to 8.5; (E) heat stability: being stable up to 60° C. at a pH of 7.4 for 30 minutes; and (F) coenzyme: flavin adenine dinucleotide (FAD).
摘要翻译：本发明提供了一种新的L-谷氨酸氧化酶，一种编码该酶的基因及其制备方法。通过使用编码该酶的基因，可以通过重组DNA技术以低成本容易地制备L-谷氨酸氧化酶。新型L-谷氨酸氧化酶具有以下物理化学性质：（A）作用：催化以下反应：<？in-line-formula description =“In-line formula”end =“lead”？> L-谷氨酸+ O 2→2 +→2→O-酮戊二酸+ H 2 O 2 + NH 3 （B）底物特异性：特异于L-谷氨酸;（B）底物特异性：（C）分子量和亚基结构：通过SDS-聚丙烯酰胺凝胶电泳测定的分子量为70,000±6,000，通过凝胶过滤测定的分子量为140,000±10,000，并且是由相同亚单位形成的二聚体，其分子量为 70,000±6,000; （D）最适pH：pH 6.0〜8.5左右; （E）热稳定性：在pH 7.4时稳定30分钟，稳定至60℃; 和（F）辅酶：黄素腺嘌呤二核苷酸（FAD）。

4. 发明申请

US20060127447A1 Poly-gamma-glutamate having ultra high molecular weight and method for using the same 审中-公开
标题翻译：具有超高分子量的聚-γ-谷氨酸盐及其使用方法
公开(公告)号：US20060127447A1
公开(公告)日：2006-06-15
申请号：US10520557
申请日：2003-07-10
申请人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Kwang Seok Kim , Jae Jun Song , Eu Gene Rha , Kwang Kim , Kenji Soda , Makoto Ashiuchi , Ha Ryoung Poo , Seung Lee
发明人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Kwang Seok Kim , Jae Jun Song , Eu Gene Rha , Kwang Kim , Kenji Soda , Makoto Ashiuchi , Ha Ryoung Poo , Seung Lee
IPC分类号： A61K38/16 , A61K47/00 , C07K14/32 , C12N1/21 , C12P21/06
CPC分类号： A61K9/0014 , A61K8/88 , A61K9/0095 , A61K47/42 , A61Q19/00 , C08H1/00 , C12P21/02
摘要： The present invention relates to a poly-gamma-glutamate (PGA) having an ultra-high molecular weight greater than 5,000 kDa. The ultra-high molecular weight PGA according to the present invention has a mean molecular weight greater than 13,000 kDa, and more than 95% of its molecules have a molecular weight ranging from 3,000 to 15,000 kDa. Also, it can be produced by the culturing of Bacillus subtilis var. chungkookjang. The ultra-high molecular weight PGA according to the present invention shows very excellent moisture-absorbing, moisture-retaining, sustained release, mineral solubility, and water-absorbing properties, and thus, can be used as a new and high value-added material in various applications.
摘要翻译：本发明涉及超高分子量大于5,000kDa的聚-γ-谷氨酸（PGA）。根据本发明的超高分子量PGA的平均分子量大于13000kDa，其分子的95％以上具有3,000至15,000kDa的分子量。此外，它可以通过培养枯草芽孢杆菌变种产生。 ung ko ang。根据本发明的超高分子量PGA显示出非常优异的吸湿性，保湿性，缓释性，矿物溶解性和吸水性，因此可用作新的高附加值材料在各种应用中。

5. 发明申请

US20100233764A1 Process for Producing Poly-y-Glutamic Acid Having High Optical Purity 有权
标题翻译：生产具有高光学纯度的聚谷氨酸的方法
公开(公告)号：US20100233764A1
公开(公告)日：2010-09-16
申请号：US12223067
申请日：2007-01-31
申请人： Makoto Ashiuchi , Kazuki Shimizu
发明人： Makoto Ashiuchi , Kazuki Shimizu
IPC分类号： C12P13/14
CPC分类号： C12R1/11 , C12P13/14 , C12P21/02
摘要： It is an objective of the present invention to provide a process for efficiently producing poly-γ-glutamic acid having a high L-glutamic acid content and excellent quality. The process for producing poly-γ-glutamic acid according to the present invention is characterized in using a bacterium belonging to species Bacillus megaterium.
摘要翻译：本发明的目的是提供一种高效生产L-谷氨酸含量高，品质优良的聚-γ-谷氨酸的方法。根据本发明的制备聚-γ-谷氨酸的方法的特征在于使用属于芽孢杆菌（Bacillus megaterium）的细菌。

6. 发明授权

US07553636B2 Surface expression vectors having pgsBCA the gene coding poly-gamma-glutamate synthetase, and a method for expression of target protein at the surface of microorganism using the vector 有权
标题翻译：具有编码聚-γ-谷氨酸合成酶的基因的pgsBCA的表达表达载体，以及使用载体在微生物表面表达靶蛋白的方法
公开(公告)号：US07553636B2
公开(公告)日：2009-06-30
申请号：US10484605
申请日：2002-08-09
申请人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Chul-Joong Kim , Kenji Soda , Makoto Ashiuchi
发明人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Chul-Joong Kim , Kenji Soda , Makoto Ashiuchi
IPC分类号： C12N15/09 , C12N1/21 , C12N15/63
CPC分类号： C12N9/1051 , C07K2319/02 , C12N9/1288 , C12Y204/01133 , C12Y207/08005
摘要： The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.
摘要翻译：本发明涉及具有pgsBCA的表达表达载体，编码聚-γ-谷氨酸合成酶的基因和使用该载体在微生物表面表达靶蛋白的方法。插入外来基因的载体转化微生物，使外源蛋白稳定表达在微生物表面。

7. 发明授权

US08703443B2 γ-L-PGA producing microorganism, method of producing γ-L-PGA using the microorganism, crosslinked substance produced using the microorganism, and external dermal agent produced using the microorganism 有权
标题翻译： γ-L-PGA生产微生物，使用微生物生产γ-L-PGA的方法，使用该微生物生产的交联物质和使用该微生物产生的外部皮肤试剂
公开(公告)号：US08703443B2
公开(公告)日：2014-04-22
申请号：US12301411
申请日：2007-03-12
申请人： Shuhei Yamamoto , Masaru Kitagawa , Michiko Suzuki , Atsushi Sogabe , Makoto Ashiuchi
发明人： Shuhei Yamamoto , Masaru Kitagawa , Michiko Suzuki , Atsushi Sogabe , Makoto Ashiuchi
IPC分类号： C12P21/06
CPC分类号： A61Q19/00 , A61K8/88 , C08G69/10 , C08L77/04 , C08L2205/02 , C12P21/02 , C08L2666/20
摘要： There are provided a microorganism characterized by producing poly-γ-L-glutamate with a molecular weight of 1,300,000 or greater and uniform optical purity under liquid culture conditions, a method of screening for the microorganism, a method of producing poly-γ-L-glutamate having large molecular weight by using the microorganism, and poly-γ-L-glutamate having an average molecular weight of 1,300,000 or greater. In addition, usages of these are provided.
摘要翻译：提供一种微生物，其特征在于在液体培养条件下生产分子量为130万以上，光学纯度高的聚-γ-L-谷氨酸，筛选微生物的方法，生产聚-γ- 通过使用微生物具有大分子量的谷氨酸盐以及平均分子量为130万以上的聚-γ-L-谷氨酸。此外，还提供了这些用途。

8. 发明授权

US07582466B2 Bacillus subtilis var. chungkookjang producing high molecular weight poly-gamma-glutamic acid 有权
标题翻译：枯草芽孢杆菌变种 chungkookjang生产高分子量聚-γ-谷氨酸
公开(公告)号：US07582466B2
公开(公告)日：2009-09-01
申请号：US11324675
申请日：2005-12-31
申请人： MoonHee Sung , DaeHeoun Baek , SeungGoo Lee , JaeJun Song , SeungPyo Hong , YoonHo Choi , SunYoung Shin , JaeSuk Han , Makoto Ashiuchi , Kenji Soda
发明人： MoonHee Sung , DaeHeoun Baek , SeungGoo Lee , JaeJun Song , SeungPyo Hong , YoonHo Choi , SunYoung Shin , JaeSuk Han , Makoto Ashiuchi , Kenji Soda
IPC分类号： C12N1/20
CPC分类号： C12R1/125 , C07K14/32
摘要： Bacillus subtilis var. chungkookjang, accession no. KCTC 0697BP, a salt-resistant B. subtilis variant isolated from “chungkookjang,” a traditional Korean fermented soy bean paste. This B. subtilis variant produces poly-gamma-glutamic acid (or poly-gamma-glutamate, γ-PGA), an edible, soluble, anionic, and biodegradable high molecular-weight polymer. Bacillus subtilis var. chungkookjang produces γ-PGA with a higher molecular weight than other extracellular γ-PGAs derived from general Bacillus sp. strains. Accordingly, the γ-PGA produced by Bacillus subtilis var. chungkookjang can be utilized for the development of high value-added products, such as cosmetics, absorption agents, and biodegradable plastic materials.
摘要翻译：枯草芽孢杆菌变种 chungkookjang，加入号 KCTC 0697BP，一种从韩国发酵大豆酱“chungkookjang”分离的耐盐性枯草芽孢杆菌变体。这种枯草芽孢杆菌变体产生聚-γ-谷氨酸（或聚-γ-谷氨酸，γ-PGA），可食用，可溶性，阴离子和可生物降解的高分子量聚合物。枯草芽孢杆菌变种 chungkookjang生产比其他来自一般芽孢杆菌属的细胞外γ-PGA更高的分子量的γ-PGA。菌株。因此，由枯草芽孢杆菌变种产生的γ-PGA chungkookjang可用于开发高附加值产品，如化妆品，吸收剂和可生物降解的塑料材料。

9. 发明申请

US20060228790A1 L-glutamate oxidase 审中-公开
标题翻译： L-谷氨酸氧化酶
公开(公告)号：US20060228790A1
公开(公告)日：2006-10-12
申请号：US11399446
申请日：2006-04-07
申请人： Kenji Inagaki , Jiro Arima , Makoto Ashiuchi , Toshiharu Yagi , Hitoshi Kusakabe
发明人： Kenji Inagaki , Jiro Arima , Makoto Ashiuchi , Toshiharu Yagi , Hitoshi Kusakabe
IPC分类号： C12N9/06 , C07H21/04 , C12P21/06 , C12N1/21
CPC分类号： C12Y104/03011 , C12N9/0022
摘要： The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique. The novel L-glutamate oxidase has the following physicochemical properties: (A) action: catalyzing the following reaction: L-glutamic acid+O2+H2O→α-ketoglutaric acid+H2O2+NH3; (B) substrate specificity: being specific to L-glutamic acid; (C) molecular weight and subunit structure: molecular weight as determined through SDS-polyacrylamide gel electrophoresis of 70,000±6,000, molecular weight as determined through gel filtration of 140,000±10,000, and being a dimer formed of the same subunits having a molecular weight of 70,000±6,000; (D) optimum pH: around pH 6.0 to 8.5; (E) heat stability: being stable up to 60° C. at a pH of 7.4 for 30 minutes; and (F) coenzyme: flavin adenine dinucleotide (FAD).
摘要翻译：本发明提供了一种新的L-谷氨酸氧化酶，一种编码该酶的基因及其制备方法。通过使用编码该酶的基因，可以通过重组DNA技术以低成本容易地制备L-谷氨酸氧化酶。新型L-谷氨酸氧化酶具有以下物理化学性质：（A）作用：催化以下反应：<？in-line-formula description =“In-line formula”end =“lead”？> L-谷氨酸+ O 2→2 +→2→O-酮戊二酸+ H 2 O 2 + NH 3 （B）底物特异性：特异于L-谷氨酸;（B）底物特异性：（C）分子量和亚基结构：通过SDS-聚丙烯酰胺凝胶电泳测定的分子量为70,000±6,000，通过凝胶过滤测定的分子量为140,000±10,000，并且是由相同亚单位形成的二聚体，其分子量为 70,000±6,000; （D）最适pH：pH 6.0〜8.5左右; （E）热稳定性：在pH 7.4时稳定30分钟，稳定至60℃; 和（F）辅酶：黄素腺嘌呤二核苷酸（FAD）。

10. 发明授权

US07091010B2 Bacillus subtilis var. chungkookjang producing high molecular weight poly-gamma-glutamic acid 有权
标题翻译：枯草芽孢杆菌变种 chungkookjang生产高分子量聚-γ-谷氨酸
公开(公告)号：US07091010B2
公开(公告)日：2006-08-15
申请号：US10204696
申请日：2001-08-11
申请人： MoonHee Sung , DaeHeoun Baek , SeungGoo Lee , JaeJun Song , SeungPyo Hong , YoonHo Choi , SunYoung Shin , JaeSuk Han , Makoto Ashiuchi , Kenji Soda
发明人： MoonHee Sung , DaeHeoun Baek , SeungGoo Lee , JaeJun Song , SeungPyo Hong , YoonHo Choi , SunYoung Shin , JaeSuk Han , Makoto Ashiuchi , Kenji Soda
IPC分类号： C12P13/14 , C12P21/02 , A61K38/04
CPC分类号： C12R1/125 , C07K14/32
摘要： Salt-resistant Bacillus subtilis var. chungkookjang, accession no. KCTC 0697BP, separated from “chungkookjang” a traditional Korean fermented soy bean paste and its poly-gamma-glutamic acid (or poly-gamma-glutamate, g-PGA), an edible, soluble, anionic, and biodegradable high molecular-weight polymer. Bacillus subtilis var. chungkookjang produces g-PGA with a higher molecular weight than other extracellular g-PGAs derived from general Bacillus sp. strains and can be utilized for the development of high value-added products, such as cosmetics, absorption agents, and biodegradable plastic materials.
摘要翻译：耐盐枯草芽孢杆菌变种 chungkookjang，加入号 KCTC 0697BP，与“chungkookjang”（韩国发酵大豆酱）及其聚-γ-谷氨酸（或聚-γ-谷氨酸，g-PGA）分开，可食用，可溶性，阴离子和可生物降解的高分子量聚合物。枯草芽孢杆菌变种 chungkookjang生产比其他来自一般芽孢杆菌属的细胞外g-PGA分子量更高的g-PGA。并可用于开发高附加值产品，如化妆品，吸收剂和可生物降解的塑料材料。

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