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1. 发明申请

US20090099079A1 Novel Fungal Enzymes 有权
标题翻译：新型真菌酶
公开(公告)号：US20090099079A1
公开(公告)日：2009-04-16
申请号：US12205694
申请日：2008-09-05
申请人： Mark A. Emalfarb , Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wihelmina Agnes Hinz , Mark Gosink , Zhijie Jiang , Jacoba Van der Meij
发明人： Mark A. Emalfarb , Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wihelmina Agnes Hinz , Mark Gosink , Zhijie Jiang , Jacoba Van der Meij
IPC分类号： A61K38/16 , C07H21/00 , C07K14/00 , C07K16/44 , C12N9/42 , C12N15/64 , C12N5/10 , C12N1/15 , C12N1/13 , C12N1/21 , C12P21/00 , C12N9/26 , C12N9/24 , C12N9/20 , C12N9/16 , C12P19/02 , C12P7/04 , C12P7/06 , D06L3/11 , C09B67/42 , C12S11/00 , C11D7/42 , D06Q1/02 , A23K1/165 , A23L1/48 , A61P29/00 , C12N1/06 , C12P13/04 , C12P7/26
CPC分类号： C12N9/248 , C07H21/00 , C12N9/2402 , C12N9/2408 , C12N9/2434 , C12N9/2471 , C12Y301/01072 , C12Y302/01023 , C12Y302/01037 , C12Y302/01055 , C12Y302/01139 , C12Y302/01151 , C12Y302/01155 , Y02E50/16 , Y02E50/17
摘要： This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.
摘要翻译：本发明涉及新型酶及其生产方法。更具体地，本发明涉及各种真菌酶。描述了编码这种酶，组合物，重组和遗传修饰的宿主细胞的核酸分子和使用方法。本发明还涉及将木质纤维素生物质转化成可降解木质纤维素材料的酶和酶的新组合（包括提供来自植物生物质的糖的协同释放的酶）的方法。本发明还涉及通过细胞壁降解释放细胞含量的方法。本发明还涉及在各种其它方法中使用新型酶和这些酶的组合物的方法，包括洗涤衣物，洗涤剂方法，生物精制，纸和纸浆的脱墨和生物漂白以及废物流的处理。

2. 发明授权

US08551751B2 BX11 enzymes having xylosidase activity 有权
标题翻译： BX11酶具有木糖苷酶活性
公开(公告)号：US08551751B2
公开(公告)日：2013-10-08
申请号：US12205694
申请日：2008-09-05
申请人： Mark A. Emalfarb , Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wihelmina Agnes Hinz , Mark Gosink , Zhijie Jiang , Jacoba Van der Meij
发明人： Mark A. Emalfarb , Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wihelmina Agnes Hinz , Mark Gosink , Zhijie Jiang , Jacoba Van der Meij
IPC分类号： C12N9/00 , C12N9/02 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/248 , C07H21/00 , C12N9/2402 , C12N9/2408 , C12N9/2434 , C12N9/2471 , C12Y301/01072 , C12Y302/01023 , C12Y302/01037 , C12Y302/01055 , C12Y302/01139 , C12Y302/01151 , C12Y302/01155 , Y02E50/16 , Y02E50/17
摘要： This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.
摘要翻译：本发明涉及新型酶及其生产方法。更具体地，本发明涉及各种真菌酶。描述了编码这种酶，组合物，重组和遗传修饰的宿主细胞的核酸分子和使用方法。本发明还涉及将木质纤维素生物质转化成可降解木质纤维素材料的酶和酶的新组合（包括提供来自植物生物质的糖的协同释放的酶）的方法。本发明还涉及通过细胞壁降解释放细胞含量的方法。本发明还涉及在各种其它方法中使用新型酶和这些酶的组合物的方法，包括洗涤衣物，洗涤剂方法，生物精制，纸和纸浆的脱墨和生物漂白以及废物流的处理。

3. 发明授权

US07923236B2 Fungal enzymes 有权
标题翻译：真菌酶
公开(公告)号：US07923236B2
公开(公告)日：2011-04-12
申请号：US11833133
申请日：2007-08-02
申请人： Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Jacoba Van der Meij, legal representative , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wihelmina Agnes Hinz , Mark Gosink , Zhijie Jiang
发明人： Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wihelmina Agnes Hinz , Mark Gosink , Zhijie Jiang
IPC分类号： C12N9/42 , C12N1/20 , C12N1/00 , C12N15/00 , C12P21/06 , C12P19/00 , C12P13/00 , C12P7/26 , C12P7/06 , C12P7/08 , C07K14/00 , C07H21/04
CPC分类号： C12Y302/01021 , C11D3/38645 , C12N9/2437 , C12N9/2442 , C12N9/2445 , C12N9/2477 , C12N9/248 , C12P7/10 , C12P19/14 , C12Y302/01003 , C12Y302/01004 , C12Y302/01014 , C12Y302/01032 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： This invention relates to enzymes and methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, deinking and biobleaching of paper and pulp, and treatment of waste streams.
摘要翻译：本发明涉及酶及其制备方法。更具体地，本发明涉及各种真菌酶。描述了编码这种酶，组合物，重组和遗传修饰的宿主细胞的核酸分子和使用方法。本发明还涉及将木质纤维素生物质转化为可发酵糖的方法，该酶用于降解木质纤维素材料和酶的新组合，包括提供来自植物生物质的糖的协同释放的酶。本发明还涉及在各种其它方法中使用新型酶和这些酶的组合物的方法，包括洗涤衣物，洗涤剂方法，脱墨和纸和纸浆的生物漂白以及废物流的处理。

4. 发明申请

US20120036599A1 NOVEL FUNGAL ENZYMES 审中-公开
公开(公告)号：US20120036599A1
公开(公告)日：2012-02-09
申请号：US13080117
申请日：2011-04-05
申请人： Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wilhelmina Agnes Hinz , Mark Gosink , Zhijie Jiang , Jacoba Van der Meij
发明人： Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wilhelmina Agnes Hinz , Mark Gosink , Zhijie Jiang , Jacoba Van der Meij
IPC分类号： A01H5/00 , C12N9/42 , C12N9/24 , C12N9/34 , C12N9/18 , C12N9/88 , C07K16/40 , C12N15/56 , C12N15/60 , C12N15/55 , C12N15/63 , C12N1/15 , C12N1/21 , C12P19/00 , C12P19/20 , C12P1/00 , C12P13/04 , C12P7/26 , C12P7/40 , C12P7/02 , C12P7/14 , C12P19/02 , C12P19/12 , C12S11/00 , C12S3/00 , A61K38/47 , A61K38/46 , A61K38/51 , A01N63/04 , A61P29/00 , A61P11/06 , A01P3/00 , A01P7/04 , D06P1/00 , C11D3/386 , A23K1/06 , A23K1/18 , C07H21/04
CPC分类号： C12Y302/01021 , C11D3/38645 , C12N9/2437 , C12N9/2442 , C12N9/2445 , C12N9/2477 , C12N9/248 , C12P7/10 , C12P19/14 , C12Y302/01003 , C12Y302/01004 , C12Y302/01014 , C12Y302/01032 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, deinking and biobleaching of paper and pulp, and treatment of waste streams.

5. 发明申请

US20120030838A1 NOVEL FUNGAL ENZYMES 审中-公开
标题翻译：新型真菌酶
公开(公告)号：US20120030838A1
公开(公告)日：2012-02-02
申请号：US13080073
申请日：2011-04-05
申请人： Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wilhelmina Agnes Hinz , Mark Gosink , Zhijie Jiang , Jacobs Van der Meij
发明人： Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wilhelmina Agnes Hinz , Mark Gosink , Zhijie Jiang , Jacobs Van der Meij
IPC分类号： A01H5/00 , C12N15/63 , C12N1/15 , C12N1/21 , C12N15/56 , C12N15/60 , C12N15/55 , C12N9/42 , C12N9/24 , C12N9/34 , C12N9/88 , C12N9/18 , C07K16/40 , C12P19/00 , C12P1/00 , C12P13/04 , C12P7/26 , C12P7/40 , C12P7/02 , C12P7/14 , C12P19/02 , C12P19/12 , C12S11/00 , A61K38/47 , A61K38/46 , A61K38/51 , A01N63/04 , A61P29/00 , A61P11/06 , A01P3/00 , A01P7/04 , C12S3/00 , C11D3/386 , C09B67/42 , A23K1/06 , A23K1/18 , C07H21/04
CPC分类号： C12Y302/01021 , C11D3/38645 , C12N9/2437 , C12N9/2442 , C12N9/2445 , C12N9/2477 , C12N9/248 , C12P7/10 , C12P19/14 , C12Y302/01003 , C12Y302/01004 , C12Y302/01014 , C12Y302/01032 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, deinking and biobleaching of paper and pulp, and treatment of waste streams.
摘要翻译：本发明涉及新型酶及其生产方法。更具体地，本发明涉及各种真菌酶。描述了编码这种酶，组合物，重组和遗传修饰的宿主细胞的核酸分子和使用方法。本发明还涉及将木质纤维素生物质转化为可发酵糖的方法，该酶用于降解木质纤维素材料和酶的新组合，包括提供来自植物生物质的糖的协同释放的酶。本发明还涉及在各种其它方法中使用新型酶和这些酶的组合物的方法，包括洗涤衣物，洗涤剂方法，脱墨和纸和纸浆的生物漂白以及废物流的处理。

6. 发明授权

US09175296B2 Fungal production system 有权
标题翻译：真菌生产系统
公开(公告)号：US09175296B2
公开(公告)日：2015-11-03
申请号：US13138661
申请日：2010-03-16
申请人： Peter J. Punt , Richard Paul Burlingame , Christine M. Pynnonen , Phillip T. Olson , Jan Wery , Johannes Heinrich Visser , Mark A. Emalfarb , Jacob Visser , Jan Cornelis Verdoes , Jacoba Verdoes
发明人： Peter J. Punt , Richard Paul Burlingame , Christine M. Pynnonen , Phillip T. Olson , Jan Wery , Johannes Heinrich Visser , Mark A. Emalfarb , Jacob Visser , Jan Cornelis Verdoes
IPC分类号： C12P21/00 , C12N15/80 , C12N1/14 , C12N9/12 , C12N9/34 , C12N15/01 , C12P21/02 , C12R1/645 , C12N9/42 , C12N9/24
CPC分类号： C12N15/80 , C12N1/14 , C12N9/1205 , C12N9/2402 , C12N9/2428 , C12N9/2437 , C12N9/2442 , C12N9/248 , C12N15/01 , C12P21/02 , C12Q1/40 , C12R1/645 , C12Y302/01003 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01014 , C12Y302/01015 , C12Y302/01091 , C12Y304/21062 , C12Y304/23025 , G01N2333/93
摘要： The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous β-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.
摘要翻译：本发明提供了一种新的真菌生产系统，其包含真菌宿主菌株Chrysosporium lucknowense，其中内源性纤维素酶分泌少于Chrysosporium lucknowense菌株UV18-25的内源性纤维素酶分泌的20％。内源性蛋白酶，内源性葡聚糖酶和内源纤维二糖水解酶的分泌优选小于Chrysosporium lucknowense菌株UV 18-25分泌的20％。此外，提供真菌宿主菌株，其中几个基因已被破坏。根据本发明的另一方面，已经描述了用于纯化高于75％的纯蛋白质的同源和/或异源产生方法，其包括在本发明的菌株中表达编码所述蛋白质的基因。此外，已经公开了生产人造蛋白质混合物的方法，其包括在根据本发明的菌株中表达编码每种所述蛋白质的基因。已经提供了通过应用所述菌株来功能性表达所需酶的菌株的简化筛选的方法。

7. 发明授权

US07205123B2 Recombinant materials for carotenoid production 有权
标题翻译：用于类胡萝卜素生产的重组材料
公开(公告)号：US07205123B2
公开(公告)日：2007-04-17
申请号：US10001192
申请日：2001-10-30
申请人： Albert Johannes Joseph Van Ooijen , Jan Cornelis Verdoes , Jan Wery
发明人： Albert Johannes Joseph Van Ooijen , Jan Cornelis Verdoes , Jan Wery
IPC分类号： C12P23/00
CPC分类号： C12P23/00 , C07K14/39 , C12N9/00 , C12N9/0004 , C12N9/0008 , C12N9/1085 , C12N9/90 , C12N15/52 , C12N15/815 , C12Y102/01012
摘要： The present invention provides recombinant DNA comprising a transcription promoter and a downstream sequence to be expressed, in operable linkage therewith, wherein the transcription promoter comprises a region found upstream of the open reading frame of a highly expressed Phaffia gene, preferably a glycolytic pathway gene, more preferably the gene coding for Glyceraldehyde-3-Phosphate Dehydrogenase. Further preferred recombinant DNAs according to the invention contain promoters of ribosomal protein encoding genes, more preferably wherein the transcription promoter comprises a region found upstream of the open reading frame encoding a protein as represented by one of the amino acid sequences depicted in any one of SEQIDNOs: 24 to 50. According to a further aspect of the invention an isolated DNA sequence coding for an enzyme involved in the carotenoid biosynthetic pathway of Phaffia rhodozyma is provided, preferably wherein said enzyme has an activity selected from isopentenyl pyrophosphate isomerase activity, geranylgeranyl pyrophosphate synthase activity, phytoene synthase activity, phytoene desaturase activity and lycopene cyclase activity, still more preferably those coding for an enzyme having an amino acid sequence selected from the one represented by SEQIDNO: 13, SEQIDNO: 15, SEQIDNO: 17, SEQIDNO: 19, SEQIDNO: 21 or SEQIDNO: 23. Further embodiments concern vectors, transformed host organisms, methods for making proteins and/or carotenoids, such as astaxanthin, and methods for isolating highly expressed promoters from Phaffia.
摘要翻译：本发明提供了包含转录启动子和待表达的下游序列的重组DNA，其可操作地连接于其中，其中转录启动子包含在高度表达的菲菲亚基因，优选糖酵解途径基因的开放阅读框的上游发现的区域，更优选为编码甘油醛-3-磷酸脱氢酶的基因。更优选的根据本发明的重组DNA包含核糖体蛋白质编码基因的启动子，更优选地，其中转录启动子包含编码如SEQ ID NO中任一个所示的氨基酸序列之一的开放阅读框上游的区域根据本发明的另一方面，提供了编码参与红发夫酵母的类胡萝卜素生物合成途径的酶的分离的DNA序列，优选其中所述酶具有选自异戊烯基焦磷酸异构酶活性，香叶基香叶基焦磷酸合酶活性，八氢番茄红素合成酶活性，八氢番茄红素去饱和酶活性和番茄红素环化酶活性，还更优选编码具有选自SEQ ID NO：13，SEQ ID NO：15，SEQ ID NO：17，SEQ ID NO：19，SEQ ID NO ：21或SEQ ID NO：23.其他实施方案涉及载体，转化宿主生物，用于制备蛋白质和/或类胡萝卜素的方法，例如虾青素，以及从Phaffia分离高表达启动子的方法。

8. 发明授权

US06329141B1 Methods for transforming Phaffia strains, transformed Phaffia strains so obtained and recombinant DNA in said methods 有权
标题翻译：用于在所述方法中转化Phaffia菌株，如此获得的转化的Phaffia菌株和重组DNA的方法
公开(公告)号：US06329141B1
公开(公告)日：2001-12-11
申请号：US09091725
申请日：1998-11-19
申请人： Albert Johannes Joseph Van Ooijen , Jan Cornelis Verdoes , Jan Wery
发明人： Albert Johannes Joseph Van Ooijen , Jan Cornelis Verdoes , Jan Wery
IPC分类号： C12Q168
CPC分类号： C12P23/00 , C07K14/39 , C12N9/00 , C12N9/0004 , C12N9/0008 , C12N9/1085 , C12N9/90 , C12N15/52 , C12N15/815 , C12Y102/01012
摘要： The present invention provides recombinant DNA comprising a transcription promoter and a downstream sequence to be expressed, in operable linkage therewith, wherein the transcription promoter comprises a region found upstream of the open reading frame of a highly expressed Phaffia gene, preferably a glycolytic pathway gene, more preferably the gene coding for Glyceraldehyde-3-Phosphate Dehydrogenase. Further preferred recombinant DNAs according to the invention contain promoters of ribosomal protein encoding genes, more preferably wherein the transcription promoter comprises a region found upstream of the open reading frame encoding a protein as represented by one of the disclosed amino acid sequences. According to a further aspect of the invention an isolated DNA sequence coding for an enzyme involved in the carotenoid biosynthetic pathway of Phaffia rhodozyma is provided.
摘要翻译：本发明提供了包含转录启动子和待表达的下游序列的重组DNA，其可操作地连接于其中，其中转录启动子包含在高度表达的菲菲亚基因，优选糖酵解途径基因的开放阅读框的上游发现的区域，更优选为编码甘油醛-3-磷酸脱氢酶的基因。更优选的根据本发明的重组DNA包含核糖体蛋白质编码基因的启动子，更优选地，其中转录启动子包含编码由所公开的氨基酸序列之一表示的蛋白质的开放阅读框上游的区域。根据本发明的另一方面，提供了编码参与红发夫酵母的类胡萝卜素生物合成途径的酶的分离的DNA序列。

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