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1. 发明申请

US20120295321A1 Pentose Transporters and Uses Thereof 审中-公开
标题翻译：戊糖转运蛋白及其用途
公开(公告)号：US20120295321A1
公开(公告)日：2012-11-22
申请号：US13509299
申请日：2010-11-12
申请人： Patricia Ann vanKuyk , Ronald Peter de Vries , Jacob Visser , Arthur Franciscus Johannes Ram , Cornelis Antonius Maria Jacobus Johannes van den Hondel , Evy Battaglia , Diana Alexandra Vivas Duarte
发明人： Patricia Ann vanKuyk , Ronald Peter de Vries , Jacob Visser , Arthur Franciscus Johannes Ram , Cornelis Antonius Maria Jacobus Johannes van den Hondel , Evy Battaglia , Diana Alexandra Vivas Duarte
IPC分类号： C12P7/10 , C12N1/19 , C12N1/15
CPC分类号： C07K14/38 , Y02E50/16
摘要： The invention relates to the production of biofuels, proteins, peptides and other value-added compounds from crude carbon sources. The inventors identified genes encoding novel pentose transporters, in particular transporters of L-arabinose and/or D-xylose. Regulation of the Aspergillus niger genes by xlnR and araR was instrumental in the identification of these genes and their substrate specificities. Provided are novel pentose transporters and their encoding nucleic acids. Also provided are host cells (over)expressing a transporter, and industrial applications thereof, for instance in biofuel production.
摘要翻译：本发明涉及从粗碳源生产生物燃料，蛋白质，肽和其他增值化合物。发明人确定了编码新戊糖转运蛋白的基因，特别是L-阿拉伯糖和/或D-木糖的转运蛋白。通过xlnR和araR调节黑曲霉基因有助于鉴定这些基因及其底物特异性。提供新的戊糖转运蛋白及其编码核酸。还提供了表达转运蛋白的宿主细胞（以上）及其工业应用，例如在生物燃料生产中。

2. 发明申请

US20110287135A1 NOVEL ARABINOHYDROLASES 审中-公开
标题翻译：新ARABINOHYDROLASES
公开(公告)号：US20110287135A1
公开(公告)日：2011-11-24
申请号：US13024184
申请日：2011-02-09
申请人： Stefan Kuehnel , Henk A. Schols , Yvonne Westphal , Sandra Hinz , Jacob Visser
发明人： Stefan Kuehnel , Henk A. Schols , Yvonne Westphal , Sandra Hinz , Jacob Visser
IPC分类号： C12P19/00 , A23L2/02 , C12N9/24 , C12G1/00 , C12P19/02 , C12N1/15
CPC分类号： C07H21/00 , C12N9/2402 , C12Y302/01055 , C12Y302/01099
摘要： The invention relates to enzymes, compositions, and methods for efficient hydrolysis of arabinans present in plant biomass. More specifically, the invention relates to arabinases and compositions comprising arabinases to improve cell wall degradation to efficiently use plant biomass for bio-energy production. The invention also relates to a method for preparing a prebiotic. The prebiotic may contain branched arabinan oligomers comprise α-(1,5)-linked arabinan backbone, and single substituted α-(1,3)-linked arabinose monomers attached to the backbone, or double substituted α-(1,2,3,5)-linked arabinose monomers attached to the backbone, or both. The invention also relates to a method for preparing fruit juice or wine. The invention also relates to a method for saccharification of a plant biomass. The invention also relates to a recombinant micro-organism genetically modified to express the enzymes of the present invention and optionally additional enzymes to achieve the disclosed methods.
摘要翻译：本发明涉及用于有效水解存在于植物生物质中的阿拉伯糖的酶，组合物和方法。更具体地，本发明涉及含有阿拉伯糖酶的阿拉伯糖酶和组合物，以改善细胞壁降解以有效地利用植物生物质进行生物能量生产。本发明还涉及制备益生元的方法。益生元可以含有包含α-（1,5）连接的阿拉伯聚糖主链的支链阿拉伯聚糖低聚物和连接到骨架上的单取代的α-（1,3）连接的阿拉伯糖单体，或双取代的α-（1,2,3 ，5）连接在骨架上的阿拉伯糖单体，或两者。本发明还涉及一种制备果汁或葡萄酒的方法。本发明还涉及植物生物质糖化的方法。本发明还涉及经遗传修饰以表达本发明的酶的重组微生物和任选的另外的酶以实现所公开的方法。

3. 发明授权

US5681732A Cloning and expression of acetyl xylan esterases from fungal origin 失效
标题翻译：从真菌来源克隆和表达乙酰木聚糖酯酶
公开(公告)号：US5681732A
公开(公告)日：1997-10-28
申请号：US291609
申请日：1994-08-17
申请人： Leendert H. De Graaff , Jacob Visser , Henriette C. Van Den Broeck , Francois Strozyk , Felix J. M. Kormelink , Johannes C. P. Boonman
发明人： Leendert H. De Graaff , Jacob Visser , Henriette C. Van Den Broeck , Francois Strozyk , Felix J. M. Kormelink , Johannes C. P. Boonman
IPC分类号： A23K20/189 , C12C5/00 , C12C5/02 , C12H1/00 , C12N1/15 , C12N1/21 , C12N9/18 , C12N15/09 , C12N15/55 , C12P19/04 , C12P19/14 , C12R1/07 , C12R1/685 , C12S3/02 , C12S3/08 , D21C5/00 , D21C9/10 , C12N15/63
CPC分类号： D21C5/005 , A23K20/189 , C12C5/004 , C12C5/02 , C12H1/003 , C12N9/18 , C12P19/04
摘要： Methods and DNA constructs are provided for the expression of a fungal acetyl xylan esterase gene in microbial hosts. A purified fungal acetyl xylan esterase is obtained which is suited for the use as an accessory enzyme in the degradation of acetylated xylans.
摘要翻译：提供方法和DNA构建体用于在微生物宿主中表达真菌乙酰木聚糖酯酶基因。获得纯化的真菌乙酰木聚糖酯酶，其适用于乙酰化木聚糖降解中的辅助酶。

4. 发明授权

US5624834A Cloning and expression of the exo-polygalacturonase gene from aspergillus 失效
标题翻译：来自曲霉的外切多聚半乳糖醛酸酶基因的克隆和表达
公开(公告)号：US5624834A
公开(公告)日：1997-04-29
申请号：US290978
申请日：1994-10-17
申请人： Margo A. Kusters-Van Someren , Yvonne Muller , Hermanus C. M. Kester , Jacob Visser , Albert J. J. Van Ooyen , Claus Rolin
发明人： Margo A. Kusters-Van Someren , Yvonne Muller , Hermanus C. M. Kester , Jacob Visser , Albert J. J. Van Ooyen , Claus Rolin
IPC分类号： C12N15/09 , C12N9/24 , C12N15/11 , C12N15/56 , C12R1/19 , C12R1/66 , C12R1/685 , C12N9/26
CPC分类号： C12Y302/01015 , C12N9/2402
摘要： The present invention discloses a DNA sequence encoding the exo-polygalacturonase gene form Aspergillus. Specifically the Aspergillus tubigensis exo-polygalacturonase gene is cloned and expressed. The invention relates to vectors comprising the exo-polygalacturonase coding sequence and to host cells transformed with such vectors. The invention further relates to the production of recombinant exo-polygalacturonase and the use of this protein.
摘要翻译： PCT No.PCT / EP93 / 03704 Sec。 371日期：1994年10月17日 102（e）日期1994年10月17日PCT 1993年12月24日PCT公布。第WO94 / 14966号公报日期：1994年7月7日本发明公开了编码曲霉的外多聚半乳糖醛酸酶基因的DNA序列。具体来说，曲霉菌外切多聚半乳糖醛酸酶基因被克隆并表达。本发明涉及包含外多聚半乳糖醛酸酶编码序列的载体和用这些载体转化的宿主细胞。本发明进一步涉及重组外切聚半乳糖醛酸酶的生产和该蛋白质的应用。

5. 发明授权

US5610046A Cloning and expression of xylanase B 失效
标题翻译：木聚糖酶B的克隆和表达
公开(公告)号：US5610046A
公开(公告)日：1997-03-11
申请号：US290979
申请日：1994-10-28
申请人： Albert J. J. van Ooyen , Leendert H. DeGraaff , Henriette C. van den Broeck , Jacob Visser
发明人： Albert J. J. van Ooyen , Leendert H. DeGraaff , Henriette C. van den Broeck , Jacob Visser
IPC分类号： C12N15/09 , C12N9/24 , C12N15/11 , C12N15/56 , C12N15/67 , C12N15/80 , C12R1/19 , C12R1/66 , C12R1/685 , D21C5/00 , D21C9/10 , C07H21/04 , C12N1/19 , C12N15/63
CPC分类号： C12Y302/01032 , C12N15/80 , C12N9/2485 , D21C5/005
摘要： Method and expression constructs are provided for the cloning of genes from fungal origin which encode polypeptides having the activity of the Aspergillus tubigensis XYL B enzyme and their overexpression in a selected microbial host cell. The present invention provides for the high level production of XYL B which may be used in a variety of industrial applications.
摘要翻译： PCT No.PCT / EP93 / 03701 Sec。 371日期：1994年10月28日 102（e）日期1994年10月28日PCT 1993年12月24日PCT公布。公开号WO94 / 14965 日期1994年7月7日提供了用于克隆来自真菌来源的基因的方法和表达构建体，其编码具有曲霉曲霉XYL B酶活性的多肽及其在选定的微生物宿主细胞中的过表达。本发明提供可用于各种工业应用的XYL B的高产量。

6. 发明申请

US20120107856A1 New Fungal Production System 有权
标题翻译：新型真菌生产系统
公开(公告)号：US20120107856A1
公开(公告)日：2012-05-03
申请号：US13138661
申请日：2010-03-16
申请人： Peter J. Punt , Richard Paul Burlingame , Christine M. Pynnonen , Phillip T. Olson , Jan Wery , Johannes Heinrich Visser , Mark A. Emalfarb , Jacob Visser , Jan Cormelis Verdoes , Jacoba Verdoes
发明人： Peter J. Punt , Richard Paul Burlingame , Christine M. Pynnonen , Phillip T. Olson , Jan Wery , Johannes Heinrich Visser , Mark A. Emalfarb , Jacob Visser , Jan Cormelis Verdoes , Jacoba Verdoes
IPC分类号： C12Q1/34 , C12N1/15 , C12N15/113 , C12N1/14 , C12P21/00
CPC分类号： C12N15/80 , C12N1/14 , C12N9/1205 , C12N9/2402 , C12N9/2428 , C12N9/2437 , C12N9/2442 , C12N9/248 , C12N15/01 , C12P21/02 , C12Q1/40 , C12R1/645 , C12Y302/01003 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01014 , C12Y302/01015 , C12Y302/01091 , C12Y304/21062 , C12Y304/23025 , G01N2333/93
摘要： The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous β-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.
摘要翻译：本发明提供了一种新的真菌生产系统，其包含真菌宿主菌株Chrysosporium lucknowense，其中内源性纤维素酶分泌少于Chrysosporium lucknowense菌株UV18-25的内源性纤维素酶分泌的20％。内源性蛋白酶，内源性葡聚糖酶和内源纤维二糖水解酶的分泌优选小于Chrysosporium lucknowense菌株UV 18-25分泌的20％。此外，提供真菌宿主菌株，其中几个基因已被破坏。根据本发明的另一方面，已经描述了用于纯化高于75％的纯蛋白质的同源和/或异源产生方法，其包括在本发明的菌株中表达编码所述蛋白质的基因。此外，已经公开了生产人造蛋白质混合物的方法，其包括在根据本发明的菌株中表达编码每种所述蛋白质的基因。已经提供了通过应用所述菌株来功能性表达所需酶的菌株的简化筛选的方法。

7. 发明授权

US06306635B1 Fungal cellulases 失效
标题翻译：真菌纤维素酶
公开(公告)号：US06306635B1
公开(公告)日：2001-10-23
申请号：US09478816
申请日：2000-01-07
申请人： Henriëtte C. Van Den Broeck , Leendert H. De Graaff , Jacob Visser , Albert J. J. Van Ooijen
发明人： Henriëtte C. Van Den Broeck , Leendert H. De Graaff , Jacob Visser , Albert J. J. Van Ooijen
IPC分类号： C12N942
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： The present invention provides polypeptides with cellulase activity, corresponding DNA sequences, vectors, transformed hosts and a method for the production of these polypeptides, which are obtainable from Aspergillus niger var. niger or Aspergillus niger var. tubigensis.
摘要翻译：本发明提供具有纤维素酶活性的多肽，相应的DNA序列，载体，转化的宿主和用于制备这些多肽的方法，其可从黑曲霉var。黑曲霉或黑曲霉变种 tubigensis。

8. 发明授权

US06190890B1 Fungal cellulases 失效
标题翻译：真菌纤维素酶
公开(公告)号：US06190890B1
公开(公告)日：2001-02-20
申请号：US08849751
申请日：1997-06-12
申请人： Henriëtte C. Van Den Broeck , Leendert H. De Graaff , Jacob Visser , Albert J. J. Van Ooijen
发明人： Henriëtte C. Van Den Broeck , Leendert H. De Graaff , Jacob Visser , Albert J. J. Van Ooijen
IPC分类号： C12P1914
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： The present invention relates to peptides with multiple enzymatic activitives: carboxymethyl cellusase(CMCase), endoglucanase and &bgr;-glucanase, corresponding nucleotides, vectors and transformed hosts, methods of preparation and use. The peptide is used in the food industry for the liquefaction of plant cell material, e.g., in vegetable or fruit juice manufacture, or as processing aids to reduce the fouling of membranes.
摘要翻译：本发明涉及具有多种酶活性的肽：羧甲基纤维素酶（CMCase），内切葡聚糖酶和β-葡聚糖酶，相应的核苷酸，载体和转化宿主，制备和使用方法。该肽在食品工业中用于植物细胞材料的液化，例如在植物或果汁制造中，或作为加工助剂以减少膜的结垢。

9. 发明授权

US6143543A Enzyme system comprising ferulic acid esterase from Aspergillus 失效
标题翻译：包含曲霉属的阿魏酸酯酶的酶体系
公开(公告)号：US6143543A
公开(公告)日：2000-11-07
申请号：US975600
申请日：1997-11-21
申请人： Birgit Michelsen , Ronald Peter De Vries , Jacob Visser , J.o slashed.rn Borch S.o slashed.e , Charlotte Horsmans Poulsen , Masoud R. Zargahi
发明人： Birgit Michelsen , Ronald Peter De Vries , Jacob Visser , J.o slashed.rn Borch S.o slashed.e , Charlotte Horsmans Poulsen , Masoud R. Zargahi
IPC分类号： A21D8/04 , A23K1/165 , C07K16/40 , C12N1/15 , C12N9/18 , C12P7/24 , C12N9/16 , C12N9/98
CPC分类号： C12P7/24 , A21D8/042 , A23K20/189 , C07K16/40 , C12N9/18
摘要： An enzyme system is described that is useful for preparing food and feed. One enzyme of that system is obtainable from Aspergillus. That enzyme has the following characteristics: a MW of from 29 kDa to 36 kDa as measured on a SDS-Phastgel (10-15%) or about 30 kDa; a pI value of about 3-4; ferulic acid esterase activity; a pH optimum of about 5; and a temperature optimum of from about 50 to about 60.degree. C.
摘要翻译：描述了一种可用于制备食物和饲料的酶系统。该系统的一种酶可从曲霉得到。该酶具有以下特征：在SDS-Phastgel（10-15％）或约30kDa下测量的29kDa至36kDa的MW; pI值约3-4; 阿魏酸酯酶活性; 最适pH约5; 温度最佳为约50至60℃。

10. 发明授权

US6066356A Arabinoxylan degrading enzymes 有权
标题翻译：阿拉伯木聚糖降解酶
公开(公告)号：US6066356A
公开(公告)日：2000-05-23
申请号：US170354
申请日：1998-10-13
申请人： Monique Josina Andrea Van Der Wouw , Albert Johannes Joseph Van Ooijen , Marcus Matheus Catharina Gielkens , Leendert Hendrik De Graaff , Jacob Visser
发明人： Monique Josina Andrea Van Der Wouw , Albert Johannes Joseph Van Ooijen , Marcus Matheus Catharina Gielkens , Leendert Hendrik De Graaff , Jacob Visser
IPC分类号： A21D20060101 , A21D8/04 , A21D8/06 , A23G1/00 , A23K20060101 , A23K1/165 , C07H21/04 , C12N20060101 , C12N1/00 , C12N1/14 , C12N1/15 , C12N5/10 , C12N9/00 , C12N9/24 , C12N9/42 , C12N11/00 , C12N15/11 , C12N15/55 , C12R1/55 , C12R1/66 , C12S3/02 , D21C3/00 , A23G2/00
CPC分类号： C12Y302/01055 , C12N9/2402 , C12R1/66 , Y10S435/913 , Y10S435/917
摘要： An arabinoxylan-degrading enzyme from Aspergillus is provided. The enzyme is active in the degradation of water-insoluble solids obtained from maize. The enzyme can be used in the preparation of animal feed compositions, human food or in industrial processes.
摘要翻译：提供来自曲霉的阿糖木聚糖降解酶。该酶在从玉米获得的水不溶性固体的降解中是有活性的。该酶可用于制备动物饲料组合物，人类食物或工业过程。

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