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1. 发明授权

US07842465B2 Immunocytostaining methods for enhanced dye ratio discrimination in rare event detection 有权
标题翻译：在罕见事件检测中增强染料比率鉴别的免疫细胞染色方法
公开(公告)号：US07842465B2
公开(公告)日：2010-11-30
申请号：US11333731
申请日：2006-01-17
申请人： Huangpin Ben Hsieh , Nicole H. Lazarus , Robert T. Krivacic , Douglas N. Curry , Richard H. Bruce
发明人： Huangpin Ben Hsieh , Nicole H. Lazarus , Robert T. Krivacic , Douglas N. Curry , Richard H. Bruce
IPC分类号： G01N33/53
CPC分类号： G01N1/30 , G01N15/1468 , G01N21/6428 , G01N21/6456 , G01N21/6458 , G01N33/56966 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2021/6482 , G01N2021/6484 , G01N2201/0833
摘要： A method is provided for preparing a sample containing potential cells of interest and of using a laser of a laser based system for novel excitation and emission collection, and data usage including use of obtained data for direct and ratio based measurements. The prepared sample is configured to emit signals having spectral characteristics sufficient to permit filtering to differentiate and eliminate most false positives from true positives among acquired imaging events, in an imaging system employing a laser spot having a range of diameters from 1 to 20 μm or greater and which excites the fluorescence in a conventional or novel manner. These filtered events may be subsequently imaged and confirmed with another higher resolution device such as a fluorescent microscope in a short amount of time.
摘要翻译：提供了一种制备包含潜在感兴趣的细胞的样品并使用基于激光的系统的激光进行新颖的激发和发射采集以及数据使用的方法，包括使用获得的数据进行直接和基于比率的测量。所制备的样品被配置为发射具有足够的光谱特性的信号，以在使用具有1至20μm或更大的直径范围的激光斑点的成像系统中进行滤波以区分并消除所获取的成像事件中的真阳性的大多数假阳性并以常规或新颖的方式激发荧光。这些过滤的事件可以随后在较短的时间内用另一种较高分辨率的装置例如荧光显微镜进行成像和证实。

2. 发明授权

US07280261B2 Method of scanning and light collection for a rare cell detector 有权
标题翻译：稀有细胞检测器的扫描和光采集方法
公开(公告)号：US07280261B2
公开(公告)日：2007-10-09
申请号：US11017440
申请日：2004-12-20
申请人： Douglas N. Curry , Richard H. Bruce , Robert T. Krivacic
发明人： Douglas N. Curry , Richard H. Bruce , Robert T. Krivacic
IPC分类号： G02B26/08
CPC分类号： G01N21/6456 , G01N21/6428 , G01N2021/6484 , G01N2201/1085
摘要： An apparatus images a surface. An imager stage has a planar surface for supporting a sample. A fiber optic bundle has a first end of parallel first fiber ends that are arranged to define an input aperture for viewing the sample on the imager stage. A distal bundle end is arranged to define an output aperture disposed away from the imager stage. A scanning radiation source scans a radiation beam along a path that is perpendicular to the sample on the imager stage. The input aperture of the fiber optic bundle receives a light signal that is produced by the radiation source scan of the imager stage sample. The light signal is transmitted to the bundle output aperture. A photodetector detects the light signal at the distal bundle end, and a processor processes the detected light.
摘要翻译：装置对表面进行成像。成像器台具有用于支撑样品的平坦表面。光纤束具有平行的第一光纤端的第一端，其布置成限定用于观察成像器台上的样品的输入孔。远侧束端布置成限定远离成像器台布置的输出孔。扫描辐射源沿着与成像器台上的样品垂直的路径扫描辐射束。光纤束的输入孔径接收由成像器台样品的辐射源扫描产生的光信号。光信号被传输到束输出孔径。光电检测器检测远侧束端处的光信号，并且处理器处理检测到的光。

3. 发明授权

US07277569B2 Apparatus and method for detecting and locating rare cells 有权
标题翻译：用于检测和定位稀有细胞的装置和方法
公开(公告)号：US07277569B2
公开(公告)日：2007-10-02
申请号：US10616366
申请日：2003-07-09
申请人： Richard H. Bruce , Douglas N. Curry , Robert T. Krivacic , Huangpin B. Hsieh
发明人： Richard H. Bruce , Douglas N. Curry , Robert T. Krivacic , Huangpin B. Hsieh
IPC分类号： G06K9/00
CPC分类号： B82Y20/00 , B82Y5/00 , B82Y10/00 , G01N15/1468 , G01N2015/0065
摘要： In accordance with one aspect of the present application, an imager and method for detecting and locating rare cells in a sample is disclosed. An imager stage supports the sample. A fiber optic bundle has a proximate bundle end of first fiber ends arranged to define an input aperture viewing the sample on the translation stage. The fiber optic bundle further has a distal bundle end of second fiber ends arranged to define an output aperture shaped differently from the input aperture and disposed away from the imager stage. A scanning radiation source is arranged in fixed relative position to the input aperture. The scanning radiation source scans a radiation beam on the sample within a viewing area of the input aperture. The radiation beam interacts with the sample to produce a light signal that is reflected, scattered, transmitted, re-emitted, or otherwise collected and received by the input aperture and transmitted via the fiber optic bundle to the output aperture. The scanning radiation source rasters the radiation beam over a selected area of the sample. A photodetector is arranged to detect the light signal at the distal bundle end, and a processor processes the detected light signals.
摘要翻译：根据本申请的一个方面，公开了一种用于检测和定位样品中的稀有细胞的成像器和方法。成像器台支持样品。光纤束具有布置成限定观察平移台上的样品的输入孔的第一光纤端的近端束端。光纤束还具有设置成限定与输入孔不同的形状的输出孔并远离成像器台设置的第二纤维端的远端束端。扫描辐射源被布置在与输入孔相对的固定相对位置。扫描辐射源在输入孔的观察区域内扫描样品上的辐射束。辐射束与样品相互作用以产生被信号反射，散射，透射，再发射或以其它方式收集和接收的光信号，并通过光纤束传输到输出孔。扫描辐射源将辐射束照射在样品的选定区域上。光检测器被设置为检测远端束端处的光信号，并且处理器处理检测到的光信号。

4. 发明授权

US07545498B2 System and method for removing auto-fluorescence through the use of multiple detection channels 有权
标题翻译：通过使用多个检测通道去除自动荧光的系统和方法
公开(公告)号：US07545498B2
公开(公告)日：2009-06-09
申请号：US11612228
申请日：2006-12-18
申请人： Robert T. Krivacic , Huangpin B. Hsieh , Douglas N. Curry , Richard H. Bruce
发明人： Robert T. Krivacic , Huangpin B. Hsieh , Douglas N. Curry , Richard H. Bruce
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , A61B5/0059 , G01N21/6456 , G01N2021/6421 , G01N2021/6482 , G01N2021/6484 , G01N2201/0833
摘要： A system and method permits for the separation of auto-fluorescence from a signal by applying a single probe to a sample, exciting the sample with a single wavelength light source, thereby emitting a light a distance from the light source. The emitted light is split, and the split light is collected into two or more distinct channels. A first channel of the distinct channels is positioned closer to the light source than a second distinct channel of the distinct channels, and the second channel is closer to the emission frequency of the single probe than is the first channel. The light collected in the first channel and the light collected in the second channel are investigated, and an output signal is generated based upon the investigation.
摘要翻译：一种系统和方法允许通过将单个探针施加到样品上来从信号中分离自体荧光，用单个波长光源激发样品，从而从光源发射一定距离的光。发射的光被分裂，并且分离的光被收集到两个或更多个不同的通道中。不同通道的第一通道定位成比不同通道的第二不同通道更靠近光源，并且第二通道比第一通道更接近单个探针的发射频率。研究在第一通道中收集的光和在第二通道中收集的光，并且基于调查产生输出信号。

5. 发明申请

US20080144009A1 SYSTEM AND METHOD FOR REMOVING AUTO-FLUORESCENCE THROUGH THE USE OF MULTIPLE DETECTION CHANNELS 有权
标题翻译：通过使用多个检测通道去除自动荧光的系统和方法
公开(公告)号：US20080144009A1
公开(公告)日：2008-06-19
申请号：US11612228
申请日：2006-12-18
申请人： Robert T. Krivacic , Huangpin B. Hsieh , Douglas N. Curry , Richard H. Bruce
发明人： Robert T. Krivacic , Huangpin B. Hsieh , Douglas N. Curry , Richard H. Bruce
IPC分类号： G01N21/00
CPC分类号： G01N21/6428 , A61B5/0059 , G01N21/6456 , G01N2021/6421 , G01N2021/6482 , G01N2021/6484 , G01N2201/0833
摘要： A system and method permits for the separation of auto-fluorescence from a signal by applying a single probe to a sample, exciting the sample with a single wavelength light source, thereby emitting a light a distance from the light source. The emitted light is split, and the split light is collected into two or more distinct channels. A first channel of the distinct channels is positioned closer to the light source than a second distinct channel of the distinct channels, and the second channel is closer to the emission frequency of the single probe than is the first channel. The light collected in the first channel and the light collected in the second channel are investigated, and an output signal is generated based upon the investigation.
摘要翻译：一种系统和方法允许通过将单个探针施加到样品上来从信号中分离自体荧光，用单个波长光源激发样品，从而从光源发射一定距离的光。发射的光被分裂，并且分离的光被收集到两个或更多个不同的通道中。不同通道的第一通道定位成比不同通道的第二不同通道更靠近光源，并且第二通道比第一通道更接近单个探针的发射频率。研究在第一通道中收集的光和在第二通道中收集的光，并且基于调查产生输出信号。

6. 发明授权

US07305112B2 Method of converting rare cell scanner image coordinates to microscope coordinates using reticle marks on a sample media 有权
标题翻译：将稀有细胞扫描仪图像坐标转换成显微镜坐标的方法，使用样品介质上的标线标记
公开(公告)号：US07305112B2
公开(公告)日：2007-12-04
申请号：US10740972
申请日：2003-12-19
申请人： Douglas N. Curry , Richard H. Bruce , Robert T. Krivacic , Huangpin B. Hsieh , Richard A. Lerner
发明人： Douglas N. Curry , Richard H. Bruce , Robert T. Krivacic , Huangpin B. Hsieh , Richard A. Lerner
IPC分类号： G06K9/00
CPC分类号： G01N15/1475 , G01N15/1468 , G01N2015/0065 , G06K9/00134 , G06K9/32 , G06T7/73 , G06T2207/30024
摘要： Provided is a method for obtaining a position of an object. A slide which carries at least one object and has reticle marks arranged at positions which form substantially a right angle, is positioned in a slide holder of a first imaging system. A first coordinate space of the imaging system is defined, and coordinates of the reticle marks in the first coordinate space are designated. A second coordinate space of a second imaging system is defined, and the coordinates of the reticle marks in the second coordinate space is designated. Using the designated coordinates of the reticle marks of the first coordinate space, the coordinate conversion parameters are computed. Thereafter, coordinates of at least one object in the first coordinate space are designated, and the first coordinate space coordinates of the object are converted into unique coordinates in a second coordinate space, using the coordinate conversion parameters.
摘要翻译：提供了一种用于获得对象的位置的方法。携带至少一个物体并且具有布置在形成基本上直角的位置处的标线标记的滑块位于第一成像系统的滑动架中。定义成像系统的第一坐标空间，并且指定第一坐标空间中的标线标记的坐标。定义第二成像系统的第二坐标空间，并且指定第二坐标空间中的标线标记的坐标。使用第一坐标空间的标线标记的指定坐标，计算坐标转换参数。此后，指定第一坐标空间中的至少一个对象的坐标，并且使用坐标转换参数将对象的第一坐标空间坐标转换为第二坐标空间中的唯一坐标。

7. 发明授权

US07282129B2 Traveling wave algorithms to focus and concentrate proteins in gel electrophoresis 有权
标题翻译：旅行波算法聚焦和浓缩蛋白质在凝胶电泳
公开(公告)号：US07282129B2
公开(公告)日：2007-10-16
申请号：US10460137
申请日：2003-06-12
申请人： Meng H. Lean , Huangpin Ben Hsieh , John S. Fitch , Armin R. Völkel , Bryan Preas , Scott Elrod , Richard H. Bruce , Eric Peeters , Frank Torres , Michael Chabinyc
发明人： Meng H. Lean , Huangpin Ben Hsieh , John S. Fitch , Armin R. Völkel , Bryan Preas , Scott Elrod , Richard H. Bruce , Eric Peeters , Frank Torres , Michael Chabinyc
IPC分类号： G01N27/447
CPC分类号： G01N27/44713
摘要： An electrophoretic cell configuration and related method are disclosed that employ oppositely directed traveling electrical waves. The waves travel across the cell and samples undergoing separation. Various strategies are used to selectively direct the movement and arrangement of the samples and resulting sample patterns.
摘要翻译：公开了采用相反方向的行进电波的电泳池配置和相关方法。波浪穿过细胞并进行分离。使用各种策略来选择性地指导样品的移动和排列以及所得样品图案。

8. 发明授权

US07126134B2 Sample manipulator 有权
公开(公告)号：US07126134B2
公开(公告)日：2006-10-24
申请号：US10921556
申请日：2004-08-19
申请人： Meng H. Lean , Francisco E. Torres , Huangpin Ben Hsieh , Armin R. Völkel , Bryan Preas , Scott A. Elrod , John S. Fitch , Richard H. Bruce
发明人： Meng H. Lean , Francisco E. Torres , Huangpin Ben Hsieh , Armin R. Völkel , Bryan Preas , Scott A. Elrod , John S. Fitch , Richard H. Bruce
IPC分类号： G21K5/10
CPC分类号： B03C1/253
摘要： A sample manipulator that utilizes electrostatic traveling waves to selectively displace one or more samples deposited on its face is disclosed. The sample manipulator enables an operator to perform a wide variety of processes upon the deposited samples. Also disclosed are strategies for separating two or more samples, focusing a sample, and passing a reagent through a sample, all conducted on the face of the sample manipulator.

9. 发明授权

US07156970B2 Distributed multi-segmented reconfigurable traveling wave grids for separation of proteins in gel electrophoresis 有权
标题翻译：分布式多分段可重构行波网格，用于在凝胶电泳中分离蛋白质
公开(公告)号：US07156970B2
公开(公告)日：2007-01-02
申请号：US10459799
申请日：2003-06-12
申请人： Meng H. Lean , Huangpin Ben Hsieh , John S. Fitch , Armin R. Völkel , Bryan Preas , Scott Elrod , Richard H. Bruce , Eric Peeters , Frank Torres , Michael Chabinyc
发明人： Meng H. Lean , Huangpin Ben Hsieh , John S. Fitch , Armin R. Völkel , Bryan Preas , Scott Elrod , Richard H. Bruce , Eric Peeters , Frank Torres , Michael Chabinyc
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44713
摘要： Various traveling wave grids and electrophoretic systems, and electrode assemblies using such grids, are disclosed. A configuration in which a voltage potential is used to load a biomolecule sample against a grid is disclosed. A unique strategy of using multiple, reconfigurable grids in such systems is also described. The strategy involves initially conducting a broad protein separation and then selectively tailoring one or more grids, and conducting one or more secondary processing operations. Related strategies and specific methods are additionally disclosed for separating samples of biomolecules and components thereof using the noted systems, assemblies, and grids.
摘要翻译：公开了各种行波网格和电泳系统以及使用这种格栅的电极组件。公开了一种使用电压电位将生物分子样品装载在电网上的结构。还描述了在这种系统中使用多个可重构网格的独特策略。该策略首先进行广泛的蛋白质分离，然后选择性地定制一个或多个网格，以及进行一个或多个二次加工操作。另外公开了使用所述系统，组件和网格分离生物分子及其组分的样品的相关策略和具体方法。

10. 发明授权

US07150813B2 Isoelectric focusing (IEF) of proteins with sequential and oppositely directed traveling waves in gel electrophoresis 有权
标题翻译：蛋白质的等电聚焦（IEF）在凝胶电泳中具有顺序和相对定向的行波
公开(公告)号：US07150813B2
公开(公告)日：2006-12-19
申请号：US10460724
申请日：2003-06-12
申请人： Meng H. Lean , Huangpin Ben Hsieh , John S. Fitch , Armin R. Völkel , Bryan Preas , Scott Elrod , Richard H. Bruce , Eric Peeters , Frank Torres , Michael Chabinyc
发明人： Meng H. Lean , Huangpin Ben Hsieh , John S. Fitch , Armin R. Völkel , Bryan Preas , Scott Elrod , Richard H. Bruce , Eric Peeters , Frank Torres , Michael Chabinyc
IPC分类号： B01D57/02
CPC分类号： G01N27/44795 , G01N27/44713
摘要： Various gel electrophoretic assemblies and techniques are disclosed for providing unique isoelectric focusing (IEF) strategies. Several particular systems, assemblies and methods are provided that significantly reduce processing time, enable the use of reduced operating voltages, and produce analytical results with improved resolution.
摘要翻译：公开了各种凝胶电泳组件和技术，用于提供独特的等电聚焦（IEF）策略。提供了几个特定的系统，组件和方法，其显着地减少了处理时间，使得能够使用降低的工作电压，并且以改进的分辨率产生分析结果。

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