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    • 2. 发明申请
    • NUCLEIC ACID ADAPTORS AND USES THEREOF
    • 核酸适配器及其用途
    • US20130261027A1
    • 2013-10-03
    • US13877203
    • 2011-09-29
    • Bin LiZhoutao ChenTanya BioracMelvin Wei
    • Bin LiZhoutao ChenTanya BioracMelvin Wei
    • C12N15/10
    • C12Q1/6876C12N9/1252C12N9/20C12N15/1093C12N15/64C12Q1/6806C12Q1/6832C12Y207/07007C12Y301/01003C12Q2521/131C12Q2525/191C12Q2527/107C12Q2537/163C12Q2563/179
    • Provided herein are compositions, systems, methods, and kits for joining together the ends of one or more polynucleotides using at least one pair of blocking oligonucleotide adaptors. Blocking oligonucleotide adaptors can be used to reduce the formation of adaptor dimers or trimers (or higher-order concatemers) which can improve the yield of desirable polynucleotide-adaptor products in any recombinant nucleic acid workflow. Blocking oligonucleotide adaptors can comprise a double-stranded oligonucleotide adaptor (duplex) having an overhang cohesive portion that anneals with a blocking oligonucleotide which can be a separate single-stranded oligonucleotide. A blocking oligonucleotide, when annealed to an overhang portion, can prevent undesirable hybridization of the overhang portion to another nucleic acid, such as the overhang portion from another blocking oligonucleotide adaptor or a polynucleotide of interest.
    • 本文提供了使用至少一对封闭寡核苷酸衔接子将一个或多个多核苷酸的末端连接在一起的组合物,系统,方法和试剂盒。 阻断寡核苷酸衔接子可用于减少适配器二聚体或三聚体(或更高级并行物)的形成,其可以改善任何重组核酸工作流程中期望的多核苷酸 - 衔接子产物的产量。 阻断寡核苷酸衔接子可以包含具有悬伸粘性部分的双链寡核苷酸衔接子(双链体),其与可以是单独的单链寡核苷酸的封闭寡核苷酸退火。 当与突出部分退火时,封闭寡核苷酸可以防止突出部分与另一种核酸(例如来自另一种封闭寡核苷酸衔接子或感兴趣的多核苷酸的突出部分)的不期望的杂交。
    • 9. 发明授权
    • Method and a device of service migration in an automatically switched optical network
    • 自动切换光网络中的方法和服务迁移的设备
    • US09380359B2
    • 2016-06-28
    • US13140283
    • 2009-12-18
    • Jianhong WuBin Li
    • Jianhong WuBin Li
    • H04Q11/00
    • H04Q11/00H04Q11/0062H04Q2011/0086H04Q2011/0088
    • A method and a device of service migration in an automatically switched optical network are disclosed in the present invention, and the method includes: when receiving a migration indication message, a migration node sending an cross information checking request to a transmission plane (S101); when receiving a cross information checking success message, the migration node performing a slot resource migration (S102); and the migration node determining whether it is the destination node of the present service migration, if it is determined to be yes, an cross connection migration is performed directly, otherwise, the cross connection migration is performed after the indication signaling, which is sent by a downstream node and carries a cross connection migration scene, is received (S103). The present invention improves the reliability of the migration from PC to SPC, and further ensures the reliability of the migration by a strict state control and state refresh mechanism.
    • 本发明公开了一种自动交换光网络中业务迁移的方法和装置,该方法包括:接收迁移指示消息时,向发送平面发送交叉信息检查请求的移动节点(S101); 当接收到交叉信息检查成功消息时,迁移节点执行时隙资源迁移(S102); 并且迁移节点确定当前服务迁移的目的地节点是否被确定为是,直接执行交叉连接迁移,否则,在指示信令之后执行交叉连接迁移,该指示信令由 接收下游节点并携带交叉连接迁移场景(S103)。 本发明提高了从PC到SPC的迁移的可靠性,并通过严格的状态控制和状态刷新机制进一步保证了迁移的可靠性。