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1. 发明申请

US20130261027A1 NUCLEIC ACID ADAPTORS AND USES THEREOF 有权
标题翻译：核酸适配器及其用途
公开(公告)号：US20130261027A1
公开(公告)日：2013-10-03
申请号：US13877203
申请日：2011-09-29
申请人： Bin Li , Zhoutao Chen , Tanya Biorac , Melvin Wei
发明人： Bin Li , Zhoutao Chen , Tanya Biorac , Melvin Wei
IPC分类号： C12N15/10
CPC分类号： C12Q1/6876 , C12N9/1252 , C12N9/20 , C12N15/1093 , C12N15/64 , C12Q1/6806 , C12Q1/6832 , C12Y207/07007 , C12Y301/01003 , C12Q2521/131 , C12Q2525/191 , C12Q2527/107 , C12Q2537/163 , C12Q2563/179
摘要： Provided herein are compositions, systems, methods, and kits for joining together the ends of one or more polynucleotides using at least one pair of blocking oligonucleotide adaptors. Blocking oligonucleotide adaptors can be used to reduce the formation of adaptor dimers or trimers (or higher-order concatemers) which can improve the yield of desirable polynucleotide-adaptor products in any recombinant nucleic acid workflow. Blocking oligonucleotide adaptors can comprise a double-stranded oligonucleotide adaptor (duplex) having an overhang cohesive portion that anneals with a blocking oligonucleotide which can be a separate single-stranded oligonucleotide. A blocking oligonucleotide, when annealed to an overhang portion, can prevent undesirable hybridization of the overhang portion to another nucleic acid, such as the overhang portion from another blocking oligonucleotide adaptor or a polynucleotide of interest.
摘要翻译：本文提供了使用至少一对封闭寡核苷酸衔接子将一个或多个多核苷酸的末端连接在一起的组合物，系统，方法和试剂盒。阻断寡核苷酸衔接子可用于减少适配器二聚体或三聚体（或更高级并行物）的形成，其可以改善任何重组核酸工作流程中期望的多核苷酸 - 衔接子产物的产量。阻断寡核苷酸衔接子可以包含具有悬伸粘性部分的双链寡核苷酸衔接子（双链体），其与可以是单独的单链寡核苷酸的封闭寡核苷酸退火。当与突出部分退火时，封闭寡核苷酸可以防止突出部分与另一种核酸（例如来自另一种封闭寡核苷酸衔接子或感兴趣的多核苷酸的突出部分）的不期望的杂交。

2. 发明授权

US09540637B2 Nucleic acid adaptors and uses thereof 有权
标题翻译：核酸适配器及其用途
公开(公告)号：US09540637B2
公开(公告)日：2017-01-10
申请号：US13877203
申请日：2011-09-29
申请人： Bin Li , Zhoutao Chen , Tanya Biorac , Melvin Shiwade Wei
发明人： Bin Li , Zhoutao Chen , Tanya Biorac , Melvin Shiwade Wei
IPC分类号： C12Q1/68 , C12P19/34 , C12N15/10
CPC分类号： C12Q1/6876 , C12N9/1252 , C12N9/20 , C12N15/1093 , C12N15/64 , C12Q1/6806 , C12Q1/6832 , C12Y207/07007 , C12Y301/01003 , C12Q2521/131 , C12Q2525/191 , C12Q2527/107 , C12Q2537/163 , C12Q2563/179
摘要： Provided herein are compositions, systems, methods, and kits for joining together the ends of one or more polynucleotides using at least one pair of blocking oligonucleotide adaptors. Blocking oligonucleotide adaptors can be used to reduce the formation of adaptor dimers or trimers (or higher-order concatemers) which can improve the yield of desirable polynucleotide-adaptor products in any recombinant nucleic acid workflow. Blocking oligonucleotide adaptors can comprise a double-stranded oligonucleotide adaptor (duplex) having an overhang cohesive portion that anneals with a blocking oligonucleotide which can be a separate single-stranded oligonucleotide. A blocking oligonucleotide, when annealed to an overhang portion, can prevent undesirable hybridization of the overhang portion to another nucleic acid, such as the overhang portion from another blocking oligonucleotide adaptor or a polynucleotide of interest.
摘要翻译：本文提供了使用至少一对封闭寡核苷酸衔接子将一个或多个多核苷酸的末端连接在一起的组合物，系统，方法和试剂盒。阻断寡核苷酸衔接子可用于减少适配器二聚体或三聚体（或更高级并行物）的形成，其可以改善任何重组核酸工作流程中期望的多核苷酸 - 衔接子产物的产量。阻断寡核苷酸衔接子可以包含具有悬伸粘性部分的双链寡核苷酸衔接子（双链体），其与可以是单独的单链寡核苷酸的封闭寡核苷酸退火。当与突出部分退火时，封闭寡核苷酸可以防止突出部分与另一种核酸（例如来自另一种封闭寡核苷酸衔接子或感兴趣的多核苷酸的突出部分）的不期望的杂交。

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