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1. 发明授权

US06541243B1 Perfusion chamber for electrophysiological testing of oocytes 失效
标题翻译：灌注室用于卵母细胞的电生理检测
公开(公告)号：US06541243B1
公开(公告)日：2003-04-01
申请号：US09586633
申请日：2000-06-05
申请人： Eric W. Harris , Thomas H. Lanthorn , Maurice A. Kildal
发明人： Eric W. Harris , Thomas H. Lanthorn , Maurice A. Kildal
IPC分类号： C12M300
CPC分类号： C12M41/46 , C12M29/10
摘要： A perfusion chamber includes a porous oocyte support structure. A continuously sloped top surface and a receiving well in the support structure entrap the underside of the oocyte, thereby localizing the cell in a predetermined fixed position within the reach of dedicated voltage-clamp microelectrodes. A test solution is delivered continuously at the top of the chamber, above the oocyte, and withdrawn from the bottom of the chamber, below the oocyte. The porosity of the support material enables the continuous perfusion of test solution around the membrane of the oocyte, including its bottom portion that is held firmly in contact with the holding well. The geometry of the holding well is selected such as to ensure the automatic and precise placement of the oocyte by gravity and to optimize the pressure distribution over its membrane, thereby minimizing the probability of rupture or other damage to the cell. The entire top surface of the support structure is sloped inward toward the substantially central well, and the bottom of the well is contoured to conform to the shape of an oocyte of average size.
摘要翻译：灌注室包括多孔卵母细胞支持结构。支撑结构中的连续倾斜的顶表面和接收孔捕获卵母细胞的下侧，从而将细胞定位在专用电压钳微电极范围内的预定固定位置。测试溶液在卵母细胞上方的室顶部连续输送，并从卵母细胞下方的腔室底部排出。支撑材料的孔隙率使得测试溶液能够连续地灌注在卵母细胞的膜周围，包括其保持牢固地与保持阱接触的底部。选择保持孔的几何形状，以确保通过重力自动和精确地放置卵母细胞，并优化其膜上的压力分布，从而最小化破裂或其他细胞损伤的可能性。支撑结构的整个顶表面朝着基本上中心的井向内倾斜，并且孔的底部被轮廓化以符合平均尺寸的卵母细胞的形状。

2. 发明授权

US5177109A 2-amino-4,5-methyleneadipic acid compounds for treatment of CNS disorders 失效
标题翻译：用于治疗中枢神经系统疾病的2-氨基-4,5-亚甲基己二酸化合物
公开(公告)号：US5177109A
公开(公告)日：1993-01-05
申请号：US732881
申请日：1991-07-19
申请人： Roberto Pellicciari , Benedetto Natalini , Maura Marinozzi , Alexis A. Cordi , Joseph B. Monahan , Thomas H. Lanthorn
发明人： Roberto Pellicciari , Benedetto Natalini , Maura Marinozzi , Alexis A. Cordi , Joseph B. Monahan , Thomas H. Lanthorn
IPC分类号： C07C229/46 , C07C233/48 , C07C271/22
CPC分类号： C07C229/46 , C07C233/48 , C07C271/22 , C07C2101/02
摘要： Compounds, compositions and methods are described for treating a CNS disorder such as a cognitive disorder, epilepsy, depression, Parkinson's disease, Alzheimer's disease, a neurodegenerative disease or neurotoxic injury. Compound of interest are 2-amino-4,5-methyleneadipic acid compounds and derivatives defined by the formula I: ##STR1## wherein R.sup.1 is selected from hydrido, alkyl, cycloalkyl, aralkyl, aryloxyalkyl, arylthioalkyl, ##STR2## with each of R.sup.2, R.sup.3, R.sup.4 and R.sup.5 being independently selected from hydrido, alkyl, cycloalkyl, aryl and aralkyl; wherein each of X and Y is independently selected from hydroxyl, alkoxy, alkylthio, amino and ##STR3## with each of R.sup.6 and R.sup.7 being independently selected from hydrido, alkyl, cycloalkyl, aryl and aralkyl; wherein any of R.sup.1 through R.sup.7 groups having a substitutable position may be substituted with one or more substituents selected from hydroxyl, halo, alkyl, haloalkyl, cyano, alkoxy, alkylthio, sulfinyl, sulfonyl, sulfinylalkyl, sulfonylalkyl, amino, acyl, acyloxy, alkoxycarbonyl and aminocarbonyl; or a pharmaceutically-acceptable salt thereof.
摘要翻译：描述了用于治疗中枢神经系统疾病如认知障碍，癫痫，抑郁症，帕金森病，阿尔茨海默病，神经变性疾病或神经毒性损伤的化合物，组合物和方法。化合物是2-氨基-4,5-亚甲基己二酸化合物和由式I定义的衍生物：其中R 1选自氢，烷基，环烷基，芳烷基，芳氧基烷基，芳硫基烷基， R2，R3，R4和R5各自独立地选自氢，烷基，环烷基，芳基和芳烷基; 其中X和Y各自独立地选自羟基，烷氧基，烷硫基，氨基和每个R 6和R 7各自独立地选自氢，烷基，环烷基，芳基和芳烷基; 其中具有可取代位置的R 1至R 7基团中的任何一个可以被一个或多个选自羟基，卤素，烷基，卤代烷基，氰基，烷氧基，烷硫基，亚磺酰基，磺酰基，亚磺酰基烷基，磺酰基烷基，氨基，酰基，酰氧基，烷氧基羰基和氨基羰基; 或其药学上可接受的盐。

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