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1. 发明申请

US20110011973A1 AIRBAG SYSTEM WITH IMPROVED PERFORMANCE FOR A WIDE RANGE OF LOADS 审中-公开
标题翻译：安全气囊系统具有改进的性能，适用于宽范围的负载
公开(公告)号：US20110011973A1
公开(公告)日：2011-01-20
申请号：US12922854
申请日：2009-03-17
申请人： Rahav Shahar , Mordechai Ben-Soussan , David Albagli , Alon Ben Moshe
发明人： Rahav Shahar , Mordechai Ben-Soussan , David Albagli , Alon Ben Moshe
IPC分类号： B64C25/58 , B60R21/239
CPC分类号： B60R21/239 , B64C27/57 , B64C2025/325 , B64D2201/00
摘要： An airbag system according to any one individual feature or combination of features as described herein.
摘要翻译：根据本文所述的任何一个单独特征或特征的组合的气囊系统。

2. 发明授权

US06177243B1 Nucleic acid sequence detection employing amplification probes 失效
标题翻译：使用扩增探针的核酸序列检测
公开(公告)号：US06177243B1
公开(公告)日：2001-01-23
申请号：US08985700
申请日：1997-12-05
申请人： David Albagli , Reuel VanAtta , Michael Wood
发明人： David Albagli , Reuel VanAtta , Michael Wood
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , B01L7/52 , B01L2300/1883 , Y10S435/81 , C12Q2525/301 , C12Q2523/101 , C12Q2537/119 , C12Q2523/313
摘要： Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Cross-linking of the stems when unbound to complementary DNA is inhibited. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. To inhibit background cross-linking, the side chains may provide for duplex formation, where a portion of the side chain binds to a different portion of the side chain or the portion of the probe homologous to the target. Also provided are kits comprising reagents, as well as automatic devices, for carrying out the subject method.
摘要翻译：提供了用于检测核酸序列的方法和组合物。特别地，使用成对的探针，其中该对在靶核酸上限定基本上连续的序列。每个对具有侧链，其形成两个侧链的茎，其非共价结合，并且当探针和样品核酸是碱基配对时能够在激活时形成交联。不结合互补DNA时，茎的交联被抑制。每个核酸最初作为单链核酸存在以允许碱基配对，使得探针结合同源的靶核酸。活化测定混合物以提供交联，双链核酸被熔化，重复碱基配对，活化和熔化的过程，足够数量的循环，以提供可检测量的交联探针。为了抑制背景交联，侧链可以提供双链体形成，其中侧链的一部分结合到与靶同源的侧链或探针部分的不同部分。还提供了用于实施本发明方法的试剂盒以及自动装置。

3. 发明授权

US06696246B1 Assays using crosslinkable immobilized nucleic acids 失效
标题翻译：使用可交联固定的核酸进行测定
公开(公告)号：US06696246B1
公开(公告)日：2004-02-24
申请号：US09379888
申请日：1999-08-23
申请人： Bingfang Huan , David Albagli , Michael L. Wood , Reuel B. Van Atta , Peter C. Cheng
发明人： Bingfang Huan , David Albagli , Michael L. Wood , Reuel B. Van Atta , Peter C. Cheng
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/6832 , C12Q1/6837 , C12Q1/6841 , C12Q2537/143 , C12Q2565/518 , C12Q2545/114 , C12Q2523/101
摘要： Improved methods for in situ hybridization assays of cellular and subcellular systems and tissue sections, and immobilization-based assay techniques such as Northern blotting, Southern blotting, dot blots, and the like, and assay techniques wherein the probes are bound to substrates are disclosed. The subject invention employs crosslinker-containing hybridization probes capable of forming covalent bonds between the probe and the target nucleic acid. Upon activation, the crosslinker will, if the probe has hybridized with its essentially complementary target, form covalent bonds with the complementary strand to covalently crosslink the probe to the target. Subsequently, stringent wash conditions may be employed to reduce background signals due to non-specific absorption or probes or targets, while retaining all crosslinked probe/target hybrids. Also disclosed are diagnostic kits for use in clinical and diagnostic laboratories.
摘要翻译：公开了用于细胞和亚细胞系统和组织切片的原位杂交测定的改进方法和基于固定化的测定技术，例如Northern印迹，Southern印迹，斑点印迹等，以及其中探针结合到底物的测定技术。本发明使用能够在探针和靶核酸之间形成共价键的含交联剂的杂交探针。激活后，如果探针与其基本上互补的靶杂交，交联剂将与互补链形成共价键，以将探针与靶共价交联。随后，可以使用严格的洗涤条件来减少由于非特异性吸收或探针或靶标引起的背景信号，同时保留所有交联的探针/靶杂交体。还公开了用于临床和诊断实验室的诊断试剂盒。

4. 发明授权

US5767259A Oligonucleotides containing base-free linking groups with photoactivatable side chains 失效
标题翻译：含有无光连接基团和可光活化侧链的寡核苷酸
公开(公告)号：US5767259A
公开(公告)日：1998-06-16
申请号：US487034
申请日：1995-06-07
申请人： David Albagli , Reuel VanAtta , Michael Wood
发明人： David Albagli , Reuel VanAtta , Michael Wood
IPC分类号： G01N33/58 , B01L7/00 , C07H21/04 , C12M1/00 , C12M1/34 , C12N15/09 , C12P19/34 , C12Q1/68 , C07H21/02 , C12N15/00
CPC分类号： C12Q1/6816 , B01L7/52 , B01L2300/1883 , Y10S435/81
摘要： Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. Also provided are kits comprising reagents, as well as automatic devices, for carrying out the subject method.
摘要翻译：提供了用于检测核酸序列的方法和组合物。特别地，使用成对的探针，其中该对在靶核酸上限定基本上连续的序列。每个对具有侧链，其形成两个侧链的茎，其非共价结合，并且当探针和样品核酸是碱基配对时能够在激活时形成交联。每个核酸最初作为单链核酸存在以允许碱基配对，使得探针结合同源的靶核酸。活化测定混合物以提供交联，双链核酸被熔化，重复碱基配对，活化和熔化的过程，足够数量的循环，以提供可检测量的交联探针。还提供了用于实施本发明方法的试剂盒以及自动装置。

5. 发明授权

US06277570B1 Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents 失效
标题翻译：使用包含非核苷酸香豆素衍生物的探针作为多核苷酸交联剂的核酸序列检测
公开(公告)号：US06277570B1
公开(公告)日：2001-08-21
申请号：US09149161
申请日：1998-09-04
申请人： Michael L. Wood , David Albagli , Reuel B. Van Atta , Peter C. Cheng , Bingfang Huan , Douglas Y. Thien
发明人： Michael L. Wood , David Albagli , Reuel B. Van Atta , Peter C. Cheng , Bingfang Huan , Douglas Y. Thien
IPC分类号： C12Q168
CPC分类号： C07D407/12 , B01L7/52 , B01L2300/1883 , C07D311/16 , C07F9/65522 , C07F9/6561 , C07H21/00 , C12Q1/682 , C12Q1/6832 , C12Q1/689 , C12Q2600/156 , C12Q2525/101 , C12Q2523/101 , C12Q2537/125 , C12Q2525/313 , C12Q2525/161
摘要： Methods and compositions are provided for detecting nucleic acid sequences. Probes comprising a crosslinking agent are combined with a sample which may comprise a target sequence which is complementary to the probe. Hybridization is allowed to occur between complementary sequences. The crosslinking agent is activated. Covalent bonds are formed between the probe and the target sequence if they are hybridized to one another. The crosslinked nucleic acids can then be detected to indicate the presence of the target sequence. Also provided are kits comprising reagents.
摘要翻译：提供了用于检测核酸序列的方法和组合物。包含交联剂的探针与可以包含与探针互补的靶序列的样品组合。杂交可以在互补序列之间进行。交联剂被活化。如果它们彼此杂交，则在探针和靶序列之间形成共价键。然后可以检测交联的核酸以指示靶序列的存在。还提供了包含试剂的试剂盒。

6. 发明授权

US06187532B1 Double-stranded conformational polymorphism analysis 失效
公开(公告)号：US06187532B1
公开(公告)日：2001-02-13
申请号：US08742376
申请日：1996-11-01
申请人： Michael Wood , Reuel Van Atta , David Albagli
发明人： Michael Wood , Reuel Van Atta , David Albagli
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , Y10S435/81 , C12Q2565/131 , C12Q2523/101 , C12Q2523/313 , C12Q2565/125
摘要： Double-stranded conformational polymorphism analysis is performed by combining a probe comprising a cross-linking agent and optionally a label with a sample having a target sequence, which may be complementary or have one or a few mismatches with respect to the probe sequence. After sufficient time for hybridization under mild or lesser stringency conditions, hybridized pairs are irradiated to induce cross-link formation by the cross-linking agent. The sample is then analyzed by denaturing gel electrophoresis where the rate of migration depends upon the degree of complementarity between the probe and the target. For corroboration, in a second experiment, the probe may be combined with the sample under high stringency conditions, where it is found that the formation of cross-linked probe/target is substantially lower for pairs having mismatches than for fully matched pairs. After cross-linking, the sample may be separated by gel electrophoresis, and the amount of cross-linked nucleic acid determined.

7. 发明授权

US6005093A Non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents 失效
标题翻译：非核苷酸香豆素衍生物作为多核苷酸交联剂
公开(公告)号：US6005093A
公开(公告)日：1999-12-21
申请号：US401630
申请日：1995-03-09
申请人： Michael L. Wood , Peter C. Cheng , Douglas Y. Thien , David Albagli
发明人： Michael L. Wood , Peter C. Cheng , Douglas Y. Thien , David Albagli
IPC分类号： C12Q1/68 , C07D311/08 , C07D311/16 , C07D407/06 , C07D407/12 , C07F9/655 , C07F9/6561 , C07H21/00 , C07H21/04
CPC分类号： C07D407/12 , C07D311/16 , C07F9/65522 , C07F9/6561 , C07H21/00
摘要： Novel coumarin derivatives comprising a coumarin moiety linked to a non-nucleosidic backbone moiety are disclosed. The resulting molecules are typically used as photoactivate cross-linking groups when incorporated into polynucleotides as replacements for one or more of the complementary nucleoside bases present in probes used in procedures involving nucleic acid hybridization reactions.
摘要翻译：公开了包含与非核苷酸主链部分连接的香豆素部分的新型香豆素衍生物。当结合到多核苷酸中时，所得分子通常用作光活化交联基团，作为存在于涉及核酸杂交反应的程序的探针中的一个或多个互补核苷碱基的替代物。

8. 发明授权

US06303799B1 Polynucleotide crosslinking agents 失效
标题翻译：多核苷酸交联剂
公开(公告)号：US06303799B1
公开(公告)日：2001-10-16
申请号：US09189294
申请日：1998-11-10
申请人： Peter C. Cheng , David Albagli , Michael L. Wood
发明人： Peter C. Cheng , David Albagli , Michael L. Wood
IPC分类号： C07D31744
CPC分类号： C07H21/00 , C07C45/64 , C07C45/71 , C07C45/74 , C07F9/2408 , C07C49/255 , C07C49/84
摘要： Novel aryl derivatives comprising an aryl olefin moiety linked to a non-nucleosidic backbone moiety are disclosed. The resulting molecules are typically used as photoactivated crosslinking groups when incorporated into oligonucleotides to crosslink nucleic acid strands.
摘要翻译：公开了包含与非核苷酸主链部分连接的芳基烯烃部分的新型芳基衍生物。当结合到寡核苷酸中以交联核酸链时，所得分子通常用作光活化交联基团。

9. 发明申请

US20100181421A1 TWO-STAGE AIRBAG INFLATION SYSTEM WITH PYROTECHNIC DELAY 审中-公开
标题翻译：两阶段气象延迟系统
公开(公告)号：US20100181421A1
公开(公告)日：2010-07-22
申请号：US12666757
申请日：2008-06-23
申请人： David Albagli , Nathan Libis
发明人： David Albagli , Nathan Libis
IPC分类号： B64D45/04 , B60R21/26 , F42B3/04 , B64C25/00
CPC分类号： B64D25/00 , B64C25/56 , B64D2201/00
摘要： A two-stage pyrotechnic gas generator for inflating an airbag has a housing with first and second chambers each having gas release apertures. The chambers are separated by a partition having an opening. Running through the gas generator is an essentially contiguous train of combustible material including a train of fast burning material passing through the first chamber to the opening, a pyrotechnic delay element associated with the opening, and a train of fast burning material within the second chamber. The first and second chambers each include a quantity of propellant deployed to be ignited by the corresponding train of fast burning material. An initiator, associated with the first chamber, is configured to initiate combustion of the essentially contiguous train of combustible material. As a result, the second quantity of propellant is ignited at a predefined delay relative to ignition of the first quantity of propellant to achieve precisely timed two-phase inflation with a single electronic initiator.
摘要翻译：用于使气囊充气的两级烟火气体发生器具有壳体，其具有分别具有气体释放孔的第一和第二腔室。室由具有开口的隔板分开。通过气体发生器运行是基本上连续的可燃材料串，包括通过第一室到开口的快速燃烧材料串，与开口相关联的烟火延迟元件以及在第二室内的快速燃烧材料串。第一和第二室各自包括一部分被部署以由相应的快速燃烧材料列车点燃的推进剂。与第一室相关联的引发器被配置为启动基本上相邻的可燃材料串的燃烧。结果，第二量的推进剂以相对于第一量推进剂的点火的预定延迟点燃，以通过单个电子引发器实现精确定时的两相充气。

10. 发明授权

US06737239B2 Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents 失效
标题翻译：使用包含非核苷酸香豆素衍生物的探针作为多核苷酸交联剂的核酸序列检测
公开(公告)号：US06737239B2
公开(公告)日：2004-05-18
申请号：US10272466
申请日：2002-10-15
申请人： Michael L. Wood , David Albagli , Reuel B. Van Atta , Douglas Y. Thien , Peter C. Cheng , Bingfang Huan
发明人： Michael L. Wood , David Albagli , Reuel B. Van Atta , Douglas Y. Thien , Peter C. Cheng , Bingfang Huan
IPC分类号： C12Q168
CPC分类号： C07D407/12 , B01L7/52 , B01L2300/1883 , C07D311/16 , C07F9/65522 , C07F9/6561 , C07H21/00 , C12Q1/682 , Y02A50/53 , Y02A50/54 , Y02A50/57 , C12Q2565/107 , C12Q2525/101 , C12Q2523/101 , C12Q2537/119
摘要： Methods and compositions are provided for detecting nucleic acid sequences. Probes comprising a crosslinking agent are combined with a sample which may comprise a target sequence which is complementary to the probe. Hybridization is allowed to occur between complementary sequences. The crosslinking agent is activated. Covalent bonds are formed between the probe and the target sequence if they are hybridized to one another. The crosslinked nucleic acids can then be detected to indicate the presence of the target sequence. Also provided are kits comprising reagents.
摘要翻译：提供了用于检测核酸序列的方法和组合物。包含交联剂的探针与可以包含与探针互补的靶序列的样品组合。杂交可以在互补序列之间进行。交联剂被活化。如果它们彼此杂交，则在探针和靶序列之间形成共价键。然后可以检测交联的核酸以指示靶序列的存在。还提供了包含试剂的试剂盒。

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