专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5767259A Oligonucleotides containing base-free linking groups with photoactivatable side chains 失效
标题翻译：含有无光连接基团和可光活化侧链的寡核苷酸
公开(公告)号：US5767259A
公开(公告)日：1998-06-16
申请号：US487034
申请日：1995-06-07
申请人： David Albagli , Reuel VanAtta , Michael Wood
发明人： David Albagli , Reuel VanAtta , Michael Wood
IPC分类号： G01N33/58 , B01L7/00 , C07H21/04 , C12M1/00 , C12M1/34 , C12N15/09 , C12P19/34 , C12Q1/68 , C07H21/02 , C12N15/00
CPC分类号： C12Q1/6816 , B01L7/52 , B01L2300/1883 , Y10S435/81
摘要： Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. Also provided are kits comprising reagents, as well as automatic devices, for carrying out the subject method.
摘要翻译：提供了用于检测核酸序列的方法和组合物。特别地，使用成对的探针，其中该对在靶核酸上限定基本上连续的序列。每个对具有侧链，其形成两个侧链的茎，其非共价结合，并且当探针和样品核酸是碱基配对时能够在激活时形成交联。每个核酸最初作为单链核酸存在以允许碱基配对，使得探针结合同源的靶核酸。活化测定混合物以提供交联，双链核酸被熔化，重复碱基配对，活化和熔化的过程，足够数量的循环，以提供可检测量的交联探针。还提供了用于实施本发明方法的试剂盒以及自动装置。

2. 发明授权

US06177243B1 Nucleic acid sequence detection employing amplification probes 失效
标题翻译：使用扩增探针的核酸序列检测
公开(公告)号：US06177243B1
公开(公告)日：2001-01-23
申请号：US08985700
申请日：1997-12-05
申请人： David Albagli , Reuel VanAtta , Michael Wood
发明人： David Albagli , Reuel VanAtta , Michael Wood
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , B01L7/52 , B01L2300/1883 , Y10S435/81 , C12Q2525/301 , C12Q2523/101 , C12Q2537/119 , C12Q2523/313
摘要： Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Cross-linking of the stems when unbound to complementary DNA is inhibited. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. To inhibit background cross-linking, the side chains may provide for duplex formation, where a portion of the side chain binds to a different portion of the side chain or the portion of the probe homologous to the target. Also provided are kits comprising reagents, as well as automatic devices, for carrying out the subject method.
摘要翻译：提供了用于检测核酸序列的方法和组合物。特别地，使用成对的探针，其中该对在靶核酸上限定基本上连续的序列。每个对具有侧链，其形成两个侧链的茎，其非共价结合，并且当探针和样品核酸是碱基配对时能够在激活时形成交联。不结合互补DNA时，茎的交联被抑制。每个核酸最初作为单链核酸存在以允许碱基配对，使得探针结合同源的靶核酸。活化测定混合物以提供交联，双链核酸被熔化，重复碱基配对，活化和熔化的过程，足够数量的循环，以提供可检测量的交联探针。为了抑制背景交联，侧链可以提供双链体形成，其中侧链的一部分结合到与靶同源的侧链或探针部分的不同部分。还提供了用于实施本发明方法的试剂盒以及自动装置。

3. 发明授权

US06590091B2 Nucleic acid sequence detection employing amplification probes 失效
公开(公告)号：US06590091B2
公开(公告)日：2003-07-08
申请号：US09757563
申请日：2001-01-09
申请人： David Albagli , Reuel VanAtta , Michael Wood
发明人： David Albagli , Reuel VanAtta , Michael Wood
IPC分类号： C07H2104
CPC分类号： C12Q1/6816 , B01L7/52 , B01L2300/1883 , Y10S435/81 , C12Q2525/301 , C12Q2523/101 , C12Q2537/119 , C12Q2523/313
摘要： Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Cross-linking of the stems when unbound to complementary DNA is inhibited. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. To inhibit background cross-linking, the side chains may provide for duplex formation, where a portion of the side chain binds to a different portion of the side chain or the portion of the probe homologous to the target. Also provided are kits comprising reagents, as well as automatic devices, for carrying out the subject method.

4. 发明授权

US6004513A Automatic device for nucleic acid sequence detection employing amplification probes 失效
标题翻译：使用扩增探针进行核酸序列检测的自动装置
公开(公告)号：US6004513A
公开(公告)日：1999-12-21
申请号：US577121
申请日：1995-12-22
申请人： David Albagli , Reuel VanAtta , Michael Wood
发明人： David Albagli , Reuel VanAtta , Michael Wood
IPC分类号： G01N33/58 , B01L7/00 , C07H21/04 , C12M1/00 , C12M1/34 , C12N15/09 , C12P19/34 , C12Q1/68 , G01N15/06
CPC分类号： C12Q1/6816 , B01L7/52 , B01L2300/1883 , Y10S435/81
摘要： Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Cross-linking of the stems when unbound to complementary DNA is inhibited. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. To inhibit background cross-linking, the side chains may provide for duplex formation, where a portion of the side chain binds to a different portion of the side chain or the portion of the probe homologous to the target. Also provided are kits comprising reagents, as well as automatic devices, for carrying out the subject method.
摘要翻译：提供了用于检测核酸序列的方法和组合物。特别地，使用成对的探针，其中该对在靶核酸上限定基本上连续的序列。每个对具有侧链，其形成两个侧链的茎，其非共价结合，并且当探针和样品核酸是碱基配对时能够在激活时形成交联。不结合互补DNA时，茎的交联被抑制。每个核酸最初作为单链核酸存在以允许碱基配对，使得探针结合同源的靶核酸。活化测定混合物以提供交联，双链核酸被熔化，重复碱基配对，活化和熔化的过程，足够数量的循环，以提供可检测量的交联探针。为了抑制背景交联，侧链可以提供双链体形成，其中侧链的一部分结合到与靶同源的侧链或探针部分的不同部分。还提供了用于实施本发明方法的试剂盒以及自动装置。

5. 发明授权

US5616464A Nucleic acid sequence detection employing amplification probes 失效
标题翻译：使用扩增探针的核酸序列检测
公开(公告)号：US5616464A
公开(公告)日：1997-04-01
申请号：US364339
申请日：1994-12-27
申请人： David Albagli , Reuel VanAtta , Michael Wood
发明人： David Albagli , Reuel VanAtta , Michael Wood
IPC分类号： B01L7/00 , C12Q1/68 , C07H21/04 , C12N15/00 , C12P19/34
CPC分类号： C12Q1/6816 , B01L7/52 , B01L2300/1883 , Y10S436/805 , Y10S436/905 , Y10T436/143333
摘要： Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently bind to is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Each of the nucleic acids is initially present as single stranded to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. Kits are provided with the appropriate reagents for carrying out the subject method.
摘要翻译：提供了用于检测核酸序列的方法和组合物。特别地，使用成对的探针，其中该对在靶核酸上限定基本上连续的序列。当探针和样品核酸是碱基配对时，每对配对具有侧链，其形成非共价结合的两个侧链的茎，当激活时能够形成交联。每个核酸最初作为单链存在以允许碱基配对，使得探针结合同源的靶核酸。活化测定混合物以提供交联，双链核酸被熔化，重复碱基配对，活化和熔化的过程，足够数量的循环，以提供可检测量的交联探针。提供试剂盒用于进行本发明方法的适当试剂。

6. 发明授权

US06573048B1 Degradable nucleic acid probes and nucleic acid detection methods 失效
标题翻译：可降解核酸探针和核酸检测方法
公开(公告)号：US06573048B1
公开(公告)日：2003-06-03
申请号：US09551305
申请日：2000-04-18
申请人： Reuel VanAtta , David Albagli , Michael L. Wood , Peter C. Cheng
发明人： Reuel VanAtta , David Albagli , Michael L. Wood , Peter C. Cheng
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , C12Q2523/313 , C12Q2523/101
摘要： Nucleic acid probes that are susceptible to chemical or enzymatic degradation are described herein. In addition, assays and methods using such probes in the detection of target nucleic acid sequences are disclosed. The target-specific hybridization region or target-complementary region of the degradable probes can be separated via a degradation process from the detectable region. The remaining portion of the degradable probes can be easily detected. The use of the degradable probes described herein improves the signal-to-noise ratio by reducing background specific or non-specific signal generation in assays and methods of nucleic acid detection.
摘要翻译：本文描述了对化学或酶降解敏感的核酸探针。此外，公开了在检测靶核酸序列中使用这种探针的测定和方法。可降解探针的靶特异性杂交区或靶互补区可以通过降解过程与可检测区分离。可以容易地检测剩余部分的可降解探针。本文所述的可降解探针的使用通过降低测定中的背景特异性信号或非特异性信号产生以及核酸检测方法来提高信噪比。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式