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1. 发明授权

US06737239B2 Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents 失效
标题翻译：使用包含非核苷酸香豆素衍生物的探针作为多核苷酸交联剂的核酸序列检测
公开(公告)号：US06737239B2
公开(公告)日：2004-05-18
申请号：US10272466
申请日：2002-10-15
申请人： Michael L. Wood , David Albagli , Reuel B. Van Atta , Douglas Y. Thien , Peter C. Cheng , Bingfang Huan
发明人： Michael L. Wood , David Albagli , Reuel B. Van Atta , Douglas Y. Thien , Peter C. Cheng , Bingfang Huan
IPC分类号： C12Q168
CPC分类号： C07D407/12 , B01L7/52 , B01L2300/1883 , C07D311/16 , C07F9/65522 , C07F9/6561 , C07H21/00 , C12Q1/682 , Y02A50/53 , Y02A50/54 , Y02A50/57 , C12Q2565/107 , C12Q2525/101 , C12Q2523/101 , C12Q2537/119
摘要： Methods and compositions are provided for detecting nucleic acid sequences. Probes comprising a crosslinking agent are combined with a sample which may comprise a target sequence which is complementary to the probe. Hybridization is allowed to occur between complementary sequences. The crosslinking agent is activated. Covalent bonds are formed between the probe and the target sequence if they are hybridized to one another. The crosslinked nucleic acids can then be detected to indicate the presence of the target sequence. Also provided are kits comprising reagents.
摘要翻译：提供了用于检测核酸序列的方法和组合物。包含交联剂的探针与可以包含与探针互补的靶序列的样品组合。杂交可以在互补序列之间进行。交联剂被活化。如果它们彼此杂交，则在探针和靶序列之间形成共价键。然后可以检测交联的核酸以指示靶序列的存在。还提供了包含试剂的试剂盒。

2. 发明授权

US06495676B1 Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents 失效
标题翻译：使用包含非核苷酸香豆素衍生物的探针作为多核苷酸交联剂的核酸序列检测
公开(公告)号：US06495676B1
公开(公告)日：2002-12-17
申请号：US09390124
申请日：1999-09-03
申请人： Michael L. Wood , David Albagli , Reuel B. Van Atta , Douglas Y. Thien , Peter C. Cheng , Bingfang Huan
发明人： Michael L. Wood , David Albagli , Reuel B. Van Atta , Douglas Y. Thien , Peter C. Cheng , Bingfang Huan
IPC分类号： C07H2100
CPC分类号： C07D407/12 , B01L7/52 , B01L2300/1883 , C07D311/16 , C07F9/65522 , C07F9/6561 , C07H21/00 , C12Q1/682 , Y02A50/53 , Y02A50/54 , Y02A50/57 , C12Q2565/107 , C12Q2525/101 , C12Q2523/101 , C12Q2537/119
摘要： Methods and compositions are provided for detecting nucleic acid sequences. Probes comprising a crosslinking agent are combined with a sample which may comprise a target sequence which is complementary to the probe. Hybridization is allowed to occur between complementary sequences. The crosslinking agent is activated. Covalent bonds are formed between the probe and the target sequence if they are hybridized to one another. The crosslinked nucleic acids can then be detected to indicate the presence of the target sequence. Also provided are kits comprising reagents.
摘要翻译：提供了用于检测核酸序列的方法和组合物。包含交联剂的探针与可以包含与探针互补的靶序列的样品组合。杂交可以在互补序列之间进行。交联剂被活化。如果它们彼此杂交，则在探针和靶序列之间形成共价键。然后可以检测交联的核酸以指示靶序列的存在。还提供了包含试剂的试剂盒。

3. 发明授权

US06277570B1 Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents 失效
标题翻译：使用包含非核苷酸香豆素衍生物的探针作为多核苷酸交联剂的核酸序列检测
公开(公告)号：US06277570B1
公开(公告)日：2001-08-21
申请号：US09149161
申请日：1998-09-04
申请人： Michael L. Wood , David Albagli , Reuel B. Van Atta , Peter C. Cheng , Bingfang Huan , Douglas Y. Thien
发明人： Michael L. Wood , David Albagli , Reuel B. Van Atta , Peter C. Cheng , Bingfang Huan , Douglas Y. Thien
IPC分类号： C12Q168
CPC分类号： C07D407/12 , B01L7/52 , B01L2300/1883 , C07D311/16 , C07F9/65522 , C07F9/6561 , C07H21/00 , C12Q1/682 , C12Q1/6832 , C12Q1/689 , C12Q2600/156 , C12Q2525/101 , C12Q2523/101 , C12Q2537/125 , C12Q2525/313 , C12Q2525/161
摘要： Methods and compositions are provided for detecting nucleic acid sequences. Probes comprising a crosslinking agent are combined with a sample which may comprise a target sequence which is complementary to the probe. Hybridization is allowed to occur between complementary sequences. The crosslinking agent is activated. Covalent bonds are formed between the probe and the target sequence if they are hybridized to one another. The crosslinked nucleic acids can then be detected to indicate the presence of the target sequence. Also provided are kits comprising reagents.
摘要翻译：提供了用于检测核酸序列的方法和组合物。包含交联剂的探针与可以包含与探针互补的靶序列的样品组合。杂交可以在互补序列之间进行。交联剂被活化。如果它们彼此杂交，则在探针和靶序列之间形成共价键。然后可以检测交联的核酸以指示靶序列的存在。还提供了包含试剂的试剂盒。

4. 发明授权

US06696246B1 Assays using crosslinkable immobilized nucleic acids 失效
标题翻译：使用可交联固定的核酸进行测定
公开(公告)号：US06696246B1
公开(公告)日：2004-02-24
申请号：US09379888
申请日：1999-08-23
申请人： Bingfang Huan , David Albagli , Michael L. Wood , Reuel B. Van Atta , Peter C. Cheng
发明人： Bingfang Huan , David Albagli , Michael L. Wood , Reuel B. Van Atta , Peter C. Cheng
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/6832 , C12Q1/6837 , C12Q1/6841 , C12Q2537/143 , C12Q2565/518 , C12Q2545/114 , C12Q2523/101
摘要： Improved methods for in situ hybridization assays of cellular and subcellular systems and tissue sections, and immobilization-based assay techniques such as Northern blotting, Southern blotting, dot blots, and the like, and assay techniques wherein the probes are bound to substrates are disclosed. The subject invention employs crosslinker-containing hybridization probes capable of forming covalent bonds between the probe and the target nucleic acid. Upon activation, the crosslinker will, if the probe has hybridized with its essentially complementary target, form covalent bonds with the complementary strand to covalently crosslink the probe to the target. Subsequently, stringent wash conditions may be employed to reduce background signals due to non-specific absorption or probes or targets, while retaining all crosslinked probe/target hybrids. Also disclosed are diagnostic kits for use in clinical and diagnostic laboratories.
摘要翻译：公开了用于细胞和亚细胞系统和组织切片的原位杂交测定的改进方法和基于固定化的测定技术，例如Northern印迹，Southern印迹，斑点印迹等，以及其中探针结合到底物的测定技术。本发明使用能够在探针和靶核酸之间形成共价键的含交联剂的杂交探针。激活后，如果探针与其基本上互补的靶杂交，交联剂将与互补链形成共价键，以将探针与靶共价交联。随后，可以使用严格的洗涤条件来减少由于非特异性吸收或探针或靶标引起的背景信号，同时保留所有交联的探针/靶杂交体。还公开了用于临床和诊断实验室的诊断试剂盒。

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