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1. 发明公开

KR1020020081756A 돼지 아미노펩티다제 엔을 이용한 돼지 유행성 설사병바이러스의 배양방법 및 그 생독 백신 有权
标题翻译：使用磷酸氨基转移酶N和其灭活疫苗培养磷酸二乙酯病毒（PEDV）的方法
公开(公告)号：KR1020020081756A
公开(公告)日：2002-10-30
申请号：KR1020010021113
申请日：2001-04-19
申请人： 녹십자수의약품(주) , 박봉균
发明人： 박봉균 , 오진식 , 송대섭
IPC分类号： C12N7/01
CPC分类号： C12N7/00 , A61K39/215 , A61K2039/552 , C12N2770/20034 , C12Y304/11002
摘要： PURPOSE: Provided are a method for culturing porcine epidemic diarrhea virus(PEDV) using porcine aminopeptidase N and an attenuated vaccine thereof, thereby the PEDV can be cultured in higher concentration and the attenuated vaccine having improved immunogenecity can be produced. CONSTITUTION: The method for culturing porcine epidemic diarrhea virus(PEDV) using porcine aminopeptidase N comprises the steps of: culturing animal cells used for proliferation of PEDV; and inoculating PEDV into the cultured animal cell in a medium containing a cell receptor of PEDV and culturing it, wherein the animal cell is a vero cell; the cell receptor of PEDV is a receptor of porcine enterocyte or porcine aminopeptidase N; the medium for proliferation of PEDV contains α-MEM, 3% tryptose, phosphate broth, 0.3% yeast extract and antibiotics-antimycotic mixture, and has pH 7.2; and the attenuated vaccine for PEDV contains PEDV and the cell receptor of PEDV.
摘要翻译：目的：提供使用猪氨基肽酶N及其减毒疫苗培养猪流行性腹泻病毒（PEDV）的方法，从而可以以更高浓度培养PEDV，并且可以产生具有改善的免疫原性的减毒疫苗。构成：使用猪氨基肽酶N培养猪流行性腹泻病毒（PEDV）的方法包括以下步骤：培养用于PEDV增殖的动物细胞; 并在含有PEDV的细胞受体的培养基中将PEDV接种到培养的动物细胞中并进行培养，其中动物细胞是vero细胞; PEDV的细胞受体是猪肠细胞或猪氨基肽酶N的受体; PEDV增殖培养基含有α-MEM，3％柠檬酸，磷酸肉汤，0.3％酵母提取物和抗生素 - 抗真菌混合物，pH值为7.2; 而PEDV的减毒疫苗含有PEDV和PEDV的细胞受体。

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