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    • 1. 发明公开
    • 룸브리쿠스 루벨루스로부터 분리된엑소-베타-1,4-글루카네이즈
    • 从LUMBRICUS RUBELLUS分离的外 - 1,4-GLUCANASE
    • KR1020020029604A
    • 2002-04-19
    • KR1020010059724
    • 2001-09-26
    • 이미영주식회사 차백신연구소
    • 이미영
    • C12N9/24
    • C12N9/244C12Y302/01006
    • PURPOSE: An exo-beta-1,4-glucanase isolated from Lumbricus rubellus is provided, which exoglucanase can effectively decompose a fiber of the paper waste, so that the fibrous wastes can be effectively reused. CONSTITUTION: The exo-beta-1,4-glucanase is isolated from Lumbricus rubellus by precipitating Lumbricus rubellus with 0 to 50% of ammonium sulfate to prepare precipitate; and sequentially subjecting the precipitate to DEAE-Sephacel ion exchange chromatography, CM-cellulose chromatography and Sephacryl S-100 gel filtration chromatography, in which the molecular weight of the exoglucanase is 71.5 kDa, the amino acid sequence of the amino terminal of the exoglucanase is Tyr-Asp-Trp-Asp-Val-Ile-Thr-Asp-Leu-Asp-Thr-Tyr-Val-Gln-Ser, and the Km value of the exoglucanase is 0.0029 mM.
    • 目的:提供从红曲霉分离的外β-1,4-葡聚糖酶,外切葡聚糖酶可有效分解造纸废纸纤维,从而可有效地再利用纤维废物。 构成:通过用0〜50%的硫酸铵沉淀红曲霉来分离来自红曲霉的外β-1,4-葡聚糖酶,制备沉淀物; 并依次进行DEAE- Sephacel离子交换层析,CM-纤维素层析和Sephacryl S-100凝胶过滤层析,其中外切葡聚糖酶的分子量为71.5kDa,外切葡聚糖酶的氨基末端的氨基酸序列为 Tyr-Asp-Trp-Asp-Val-Ile-Thr-Asp-Leu-Asp-Thr-Tyr-Val-Gln-Ser,外切葡聚糖酶的Km值为0.0029mM。
    • 4. 发明授权
    • 모자반 또는 톳의 효소분해물 및 이의 제조방법
    • 聚合物和粘合剂的合成树脂及其制备方法
    • KR100810134B1
    • 2008-03-06
    • KR1020060086913
    • 2006-09-08
    • 에스티바이오스 주식회사
    • 배태진김기웅최옥수김지만
    • C12P21/06
    • C12P1/00A23L17/60A23V2002/00A23V2200/318A61K8/97A61Q19/00C12Y302/01004C12Y302/01006C12Y302/01091C12Y302/01099C12Y402/02003
    • A method for preparing an enzymatic lysate of gulfweed and bundle is provided to liquefy more than 80% of the gulfweed and bundle and obtain the lysate which is not gelated even if it is concentrated into 40% and is usefully used as a food composition and a cosmetic composition due to high fiber content. A method for preparing an enzymatic lysate of gulfweed and bundle comprises the steps of: (a) washing and desalting the gulfweed or bundle; (b) adding a first enzyme consisting of endo-beta1,4-glucanase, alginic acid lyase and exocellulose to the gulfweed or bundle to decompose firstly the gulfweed or bundle under pH of 4-7.5 at a temperature of 30-60 deg.C for 10-13 hours; and (c) adding a second enzyme consisting of arabanase, cellulase and beta1,4-glucanase to the material obtained from the step(b) to decompose secondly it under pH of 4-7.5 at a temperature of 30-60 deg.C for 4-6 hours. The method further comprises a step of heat-treating the material obtained from the step(c) and then filtering the heat-treated material.
    • 提供了一种制备草甘膦和束的酶裂解物的​​方法,用于液化80%以上的草甘膦和束,并获得即使将其浓缩成40%并且可用作食品组合物也不凝胶化的裂解物, 化妆品组成由于纤维含量高。 制备草甘膦和束的酶裂解物的​​方法包括以下步骤:(a)洗涤和脱盐草甘膦或束; (b)在棉花籽粒或束中加入由内切-β1,4-葡聚糖酶,藻酸裂解酶和外切纤维素组成的第一酶,首先在温度为30-60℃的4-7.5 pH值下首先分解草甘膦 10-13小时; 和(c)将由阿拉伯糖酶,纤维素酶和β1,4-葡聚糖酶组成的第二酶加入到从步骤(b)获得的材料中,在30-60℃的温度下,在4-7.5的pH值下将其分解, 4-6小时。 该方法还包括对从步骤(c)获得的材料进行热处理然后过滤热处理的材料的步骤。
    • 5. 发明公开
    • 바실러스 속 균주 유래의 신규한 β-1,3-글루카나아제 및이를 코드하는 유전자
    • 来自BACILLUS SP的BETA-1,3-GLUCANASE。 菌株和基因编码它们
    • KR1020030056761A
    • 2003-07-04
    • KR1020010087050
    • 2001-12-28
    • 학교법인 인제학원
    • 이동석김동욱김기훈고봉선
    • C12N9/24
    • C12N9/244C12Y302/01006
    • PURPOSE: A beta-1,3-glucanase from a Bacillus sp. strain and a gene encoding the same enzyme are provided, thereby mass-producing the beta-1,3-glucanase. CONSTITUTION: A beta-1,3-glucanase from the Bacillus sp. strain has the amino acid sequence of SEQ ID NO: 2 and decomposes beta-glucan. A gene encoding the beta-1,3-glucanase has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector pLK440 contains the gene of SEQ ID NO: 1. A method for producing the beta-1,3-glucanase of SEQ ID NO: 2 comprises the steps of: (a) inserting the gene encoding the beta-13-glucanase into a vector containing the expression control sequence, so that the expression control sequence can be operatively linked to the beta-13-glucanase gene; (b) transforming a host cell with the recombinant vector; (c) culturing the transformed cell in an appropriate medium under appropriate conditions; and (d) recovering the beta-1,3-glucanase from the cultured medium.
    • 目的:来自芽孢杆菌属的β-1,3-葡聚糖酶 提供了菌株和编码相同酶的基因,从而大量生产β-1,3-葡聚糖酶。 构成:来自芽孢杆菌属的β-1,3-葡聚糖酶 菌株具有SEQ ID NO:2的氨基酸序列并分解β-葡聚糖。 编码β-1,3-葡聚糖酶的基因具有SEQ ID NO:1的核苷酸序列。重组载体pLK440含有SEQ ID NO:1的基因。产生SEQ ID NO:1的β-1,3-葡聚糖酶的方法 SEQ ID NO:2包括以下步骤:(a)将编码β-13-葡聚糖酶的基因插入含有表达控制序列的载体中,使得表达调控序列可操作地连接于β-13-葡聚糖酶基因; (b)用重组载体转化宿主细胞; (c)在合适的条件下在合适的培养基中培养转化的细胞; 和(d)从培养基中回收β-1,3-葡聚糖酶。